Quantification of the adenylate cyclase toxin of Bordetella pertussis in vitro and during respiratory infection.

abstract：:Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated i...

journal_title：Infection and immunity

authors： Galgiani JN,Hewlett EL,Friedman RL

更新日期：1988-04-01 00:00:00

abstract：:Cholera toxin has been widely used to obtain insight into the cellular dynamics of the antigen-specific mucosal immune response. The present study was undertaken to clarify the influence of the organized intestinal lymphoid tissue (Peyer's patches [PP]) on the distribution of anti-cholera-toxin-containing cells (ACC) ...

journal_title：Infection and immunity

authors： Enders GA,Delius M,Ballhaus S,Brendel W

更新日期：1987-09-01 00:00:00

abstract：:The purpose of the present study was to determine the extent of immunologic responses, particularly immunopathologic responses, within the upper and lower respiratory tracts after intranasal immunization using the mucosal adjuvant cholera toxin (CT). BALB/c mice were nasally immunized with influenza virus vaccine comb...

journal_title：Infection and immunity

authors： Hodge LM,Marinaro M,Jones HP,McGhee JR,Kiyono H,Simecka JW

更新日期：2001-04-01 00:00:00

abstract：:FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...

journal_title：Infection and immunity

authors： Brumshagen C,Maus R,Bischof A,Ueberberg B,Bohling J,Osterholzer JJ,Ogunniyi AD,Paton JC,Welte T,Maus UA

更新日期：2012-12-01 00:00:00

abstract：:We have determined the amounts of specific anti-class 1 outer membrane protein antibodies in sera from 25 patients during the course of systemic meningococcal disease, using purified class 1 protein as the sensitizing antigen in an enzyme-linked immunosorbent assay. The class 1 protein was obtained from a variant of s...

journal_title：Infection and immunity

authors： Guttormsen HK,Wetzler LM,Solberg CO

更新日期：1994-04-01 00:00:00

abstract：:Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...

journal_title：Infection and immunity

authors： Runnels PL,Moon HW

更新日期：1984-09-01 00:00:00

abstract：:A concentrated bacterial culture supernatant from the hemolytic Moraxella bovis strain UQV 148NF was used to immunize mice and generate monoclonal antibodies (MAbs). One, MAb G3/D7, neutralized the hemolytic activity of M. bovis and recognized a 94-kDa protein by Western blot analysis in hemolytic M. bovis strains rep...

journal_title：Infection and immunity

authors： Billson FM,Harbour C,Michalski WP,Tennent JM,Egerton JR,Hodgson JL

更新日期：2000-06-01 00:00:00

abstract：:The intravenous administration of hydrogen peroxide has been reported to benefit patients with pneumonia and to reduce Plasmodium parasitemia in experimentally infected mice. We assessed the antibacterial activity of intravenously infused hydrogen peroxide against hydrogen peroxide-susceptible Escherichia coli (MBC of...

journal_title：Infection and immunity

authors： Shenep JL,Stokes DC,Hughes WT

更新日期：1985-06-01 00:00:00

abstract：:We have developed a rapid, continuous method for real-time monitoring of biofilms, both in vitro and in a mouse infection model, through noninvasive imaging of bioluminescent bacteria colonized on Teflon catheters. Two important biofilm-forming bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, wer...

journal_title：Infection and immunity

authors： Kadurugamuwa JL,Sin L,Albert E,Yu J,Francis K,DeBoer M,Rubin M,Bellinger-Kawahara C,Parr TR Jr,Contag PR

更新日期：2003-02-01 00:00:00

abstract：:In order to construct an effector strain for the replacement therapy of dental caries, we wished to combine the properties of low-level acid production and high-level colonization potential in a strain of Streptococcus mutans. To this end, we made a deletion in the lactate dehydrogenase (LDH) gene cloned from the bact...

journal_title：Infection and immunity

authors： Hillman JD,Chen A,Duncan M,Lee SW

更新日期：1994-01-01 00:00:00

abstract：:Ehrlichia chaffeensis is a recently isolated minute gram-negative obligatory intracellular bacterium of monocytes/macrophages and is the etiologic agent of human monocytic ehrlichiosis. It is not known how macrophages respond when they encounter ehrlichiae in terms of cytokine production. In this study, we examined cy...

journal_title：Infection and immunity

authors： Lee EH,Rikihisa Y

更新日期：1996-10-01 00:00:00

abstract：:The capacity of the intracellular pathogen Listeria monocytogenes to activate the alternative pathway of human complement was examined. Incubation of L. monocytogenes with human serum in optimal conditions (20% Mg2+EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid]-chelated serum) consumed (3...

journal_title：Infection and immunity

authors： Croize J,Arvieux J,Berche P,Colomb MG

更新日期：1993-12-01 00:00:00

abstract：:An established cell line (Vero) defective in interferon production was used to evaluate the role of interferon in chronic rubella virus infections of cell cultures. Inoculation of Vero cells with a low multiplicity of virus resulted in the development of carrier cultures which had the characteristics of a regulated in...

journal_title：Infection and immunity

authors： Mifune K,Desmyter J,Rawls WE

更新日期：1970-08-01 00:00:00

abstract：:Burkholderia pseudomallei is a Gram-negative rod and the causative agent of melioidosis, an emerging infectious disease of tropical and subtropical areas worldwide. B. pseudomallei harbors a remarkable number of virulence factors, including six type VI secretion systems (T6SS). Using our previously described plaque as...

journal_title：Infection and immunity

authors： Hopf V,Göhler A,Eske-Pogodda K,Bast A,Steinmetz I,Breitbach K

更新日期：2014-05-01 00:00:00

abstract：:Attempts were made to isolate an antigen(s) from Candida albicans suitable for detecting hypersensitivity in a murine model of candidiasis. Using footpad reactivity in cutaneously infected animals as the assay, comparisons were made of two commercial extracts and cell wall and cytoplasmic preparations made in the labo...

journal_title：Infection and immunity

authors： Domer JE,Moser SA

更新日期：1978-04-01 00:00:00

abstract：:Tuberculous pleurisy allows the study of human cells at the site of active Mycobacterium tuberculosis infection. In this study, we found that among pleural fluid (PF) lymphocytes, natural killer (NK) cells are a major source of early gamma interferon (IFN-gamma) upon M. tuberculosis stimulation, leading us to investig...

journal_title：Infection and immunity

authors： Schierloh P,Yokobori N,Alemán M,Landoni V,Geffner L,Musella RM,Castagnino J,Baldini M,Abbate E,de la Barrera SS,Sasiain MC

更新日期：2007-11-01 00:00:00

abstract：:Burkholderia cepacia is an opportunistic pathogen that causes severe systemic infections in patients with chronic granulomatous disease (CGD) or with cystic fibrosis (CF), but its mechanisms of virulence are poorly understood. We developed a murine model of systemic infection in wild-type (WT) and gamma interferon kno...

journal_title：Infection and immunity

authors： Speert DP,Steen B,Halsey K,Kwan E

更新日期：1999-08-01 00:00:00

abstract：:Subcutaneous infection chambers in rabbits were infected with a strain of Staphylococcus aureus isolated from a patient with toxic shock syndrome. Estrogens (mestranol and 17-beta-estradiol) protected male rabbits and prolonged survival. Neither androgens (testosterone and dihydrotestosterone) nor progesterone affecte...

journal_title：Infection and immunity

authors： Best GK,Abney TO,Kling JM,Kirkland JJ,Scott DF

更新日期：1986-04-01 00:00:00

abstract：:The ability of some typical enteropathogenic Escherichia coli (EPEC) strains to adhere to, invade, and increase interleukin-8 (IL-8) production in intestinal epithelial cells in vitro has been demonstrated. However, few studies regarding these aspects have been performed with atypical EPEC (aEPEC) strains, which are e...

journal_title：Infection and immunity

authors： Sampaio SC,Gomes TA,Pichon C,du Merle L,Guadagnini S,Abe CM,Sampaio JL,Le Bouguénec C

更新日期：2009-10-01 00:00:00

abstract：:Nontypeable Haemophilus influenzae (NTHI) is an important etiological agent of otitis media (OM) and of exacerbated chronic obstructive pulmonary diseases (COPD). Inflammation is a hallmark of both diseases. Interleukin-8 (IL-8), one of the important inflammatory mediators, is induced by NTHI and may play a significan...

journal_title：Infection and immunity

authors： Wang B,Cleary PP,Xu H,Li JD

更新日期：2003-10-01 00:00:00

abstract：:Piglets suckled by dams that had been vaccinated with K88 antigen were significantly more resistant to deaths caused by neonatal diarrhea after challenge with a large dose of a K88-positive enteropathogenic strain of Escherichia coli than piglets suckled by control dams. The factors most likely to be involved in prote...

journal_title：Infection and immunity

authors： Rutter JM,Jones GW,Brown GT,Burrows MR,Luther PD

更新日期：1976-03-01 00:00:00

abstract：:Studies on the innate immune response against microbial infections in Drosophila melanogaster involve mutant strains and their reference strains that act as experimental controls. We used five standard D. melanogaster laboratory reference strains (Oregon R, w1118, Canton-S, Cinnabar Brown, and Yellow White [YW]) and i...

journal_title：Infection and immunity

authors： Eleftherianos I,More K,Spivack S,Paulin E,Khojandi A,Shukla S

更新日期：2014-10-01 00:00:00

abstract：:The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial ...

journal_title：Infection and immunity

authors： Fu Y,Baumann M,Kosma P,Brade L,Brade H

更新日期：1992-04-01 00:00:00

abstract：:T cells are important in systemic anticryptococcal defenses, but a role in controlling an initial pulmonary infection has not been demonstrated. A murine model with intratracheal inoculation was developed to study the acquisition and expression of pulmonary T cell-mediated immunity against Cryptococcus neoformans. Inf...

journal_title：Infection and immunity

authors： Huffnagle GB,Yates JL,Lipscomb MF

更新日期：1991-04-01 00:00:00

abstract：:Length and restriction site polymorphism within a 2.8-kb threonine-rich open reading frame from Cryptosporidium parvum was identified and used to determine the genotypes of isolates from calves and humans. In agreement with observations of other genetic loci, all calf isolates were identical at this locus. In contrast...

journal_title：Infection and immunity

authors： Carraway M,Tzipori S,Widmer G

更新日期：1997-09-01 00:00:00

abstract：:In nature, mixed Borrelia burgdorferi infections are common and possibly can be acquired by either superinfection or coinfection. Superinfection by heterologous B. burgdorferi strains has been established experimentally, although the ability of homologous B. burgdorferi clones to superinfect a host has not been studie...

journal_title：Infection and immunity

authors： Rogovskyy AS,Bankhead T

更新日期：2014-11-01 00:00:00

abstract：:To study the immunological activity of proteins secreted by Mycobacterium tuberculosis, we carried out comparative studies in guinea pigs infected intravenously with 2.5 x 10(3) CFU of this organism or with 2.5 x 10(4) CFU of Mycobacterium bovis BCG. Groups of infected guinea pigs were skin tested with fractions of se...

journal_title：Infection and immunity

authors： Hasløv K,Andersen A,Nagai S,Gottschau A,Sørensen T,Andersen P

更新日期：1995-03-01 00:00:00

abstract：:G protein-coupled receptor kinase 5 (GRK5) is a serine/threonine kinase previously shown to mediate polymicrobial sepsis-induced inflammation. The goal of the present study was to examine the role of GRK5 in monomicrobial pulmonary infection by using an intratracheal Escherichia coli infection model of pneumonia. We u...

journal_title：Infection and immunity

authors： Packiriswamy N,Steury M,McCabe IC,Fitzgerald SD,Parameswaran N

更新日期：2016-04-22 00:00:00

abstract：:We show here that Helicobacter pylori broth culture supernatants disrupt the actin cytoskeleton of epithelial cell lines, leading to cell rounding and apoptosis through anoikis. We demonstrate that there are marked quantitative differences between strains and that there are different cell line sensitivities. By constr...

journal_title：Infection and immunity

authors： Bebb JR,Letley DP,Rhead JL,Atherton JC

更新日期：2003-06-01 00:00:00

abstract：:Borrelia burgdorferi, the causative agent of Lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. These plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown....

journal_title：Infection and immunity

authors： Bestor A,Stewart PE,Jewett MW,Sarkar A,Tilly K,Rosa PA

更新日期：2010-06-01 00:00:00