Abstract:
:After lysing the phagocytic vacuole, Shigella spp. accumulate filaments of polymerized actin on their surface at one pole, leading to the formation of actin tails that enable them to move through the cytoplasm. We have recently demonstrated that the Shigella protein IcsA is located at the pole that is adjacent to the growing end of the actin tail (M. B. Goldberg, O. Barzu, C. Parsot, and P. J. Sansonetti, J. Bacteriol. 175:2189-2196, 1993). Not every bacterium that is observed within the cytoplasm has an actin tail. The factors that determine when a bacterium will form a tail are unknown. Here we demonstrate that at the moment of initiation of movement, Shigella spp. are frequently in the process of division. Furthermore, the expression of IcsA on the surface of the bacteria occurs in a growth phase-dependent fashion, suggesting that the surface expression of IcsA per se determines the observed association of bacterial division with movement.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Goldberg MB,Theriot JA,Sansonetti PJdoi
10.1128/IAI.62.12.5664-5668.1994subject
Has Abstractpub_date
1994-12-01 00:00:00pages
5664-8issue
12eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::The identification of genes with increased expression in vivo may lead to the identification of novel or unrecognized virulence traits and/or recognition of environmental signals involved in modulating gene expression. Our laboratory is studying an extraintestinal isolate of Escherichia coli as a model pathogen. We ha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5306-5314.1999
更新日期:1999-10-01 00:00:00
abstract::Several trivalent cations, including lanthanum (La3+), inhibited the secretion (enterosorption) induced by the enterotoxins of Vibrio cholerae and Escherichia coli in the rabbit ileum in vivo. High concentrations (greater than 10 mM) of La3+ were required to inhibit cholera enterotoxin (CE)-induced enterosorption, pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1038-1044.1975
更新日期:1975-05-01 00:00:00
abstract::A group of insertion mutants was used to define the genes of plasmid pDC5 required for the expression of mannose-resistant fimbriae. Minicell experiments identified four polypeptides (71,000, 45,000, 27,000, and 17,000 daltons) concerned with fimbrial production, the smallest of these being the fimbrial subunit. The a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.900-906.1983
更新日期:1983-12-01 00:00:00
abstract::Fibrosis is a significant complication of intestinal disorders associated with microbial dysbiosis and pathobiont expansion, notably Crohn's disease (CD). Mechanisms that favor fibrosis are not well understood, and therapeutic strategies are limited. Here we demonstrate that colitis-susceptible Il10-deficient mice dev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00587-19
更新日期:2019-10-18 00:00:00
abstract::The primary antibody response of BALB/c splenocytes to sheep erythrocytes in vitro was suppressed by infection with Friend leukemia virus (FLV), with the response capacity decreasing with increasing duration of infection. The acquisition of normal antibody responses was amplified by macrophage-produced antibody respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1260-1264.1983
更新日期:1983-03-01 00:00:00
abstract::Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination of inhaled Cryptococcus neoformans from the lung to the brain. The mechanism(s) by which C. neoformans crosses the blood-brain barrier (BBB) is a key unresolved issue in cryptococcosis. We used both an in vivo mouse model and an in vit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.4985-4995.2004
更新日期:2004-09-01 00:00:00
abstract::Minicells are small, anucleate cells resulting from aberrant cell divisions at the polar ends of bacilli. We have isolated minicell-producing mutant strains of Shigella flexneri 2a (MC-I) and Shigella dysenteriae 1 (MC-V) after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Microscopically, broth cultures of M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.297-302.1980
更新日期:1980-10-01 00:00:00
abstract::In bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4005-4014.1994
更新日期:1994-09-01 00:00:00
abstract::Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1529-1534.1995
更新日期:1995-04-01 00:00:00
abstract::Antiprotein monoclonal antibodies derived from mice inoculated with Rickettsia rickettsii heated at 56 degrees C for 15 min are of two types: one is type specific for epitopes denatured by moderate temperatures, and the other is specific for epitopes resistant to 100 degrees C for 5 min. The heat-resistant epitopes ar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.825-827.1987
更新日期:1987-03-01 00:00:00
abstract::Salmonella pathogenicity island 12 (SPI-12) of Salmonella enterica serovar Typhimurium is a 15-kb region that encompasses genes STM2230 to STM2245 and encodes a remnant phage known to contribute to bacterial virulence. In mouse infection experiments and replication assays in macrophages, we demonstrated a role for fou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00067-13
更新日期:2013-07-01 00:00:00
abstract::Although cryptococcosis is characterized as a chronic central nervous system disease, it is generally accepted that the lungs are the primary portal of entry for the etiological agent. Despite this, there is a distinct lack of evidence that viable airborne particles of Cryptococcus neoformans are small enough to reach...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.3.634-638.1977
更新日期:1977-09-01 00:00:00
abstract::The effect of ingestion of heat-killed Histoplasma capsulatum yeast cells on the metabolism of arachidonic acid (20:4) to prostenoids and leukotrienes was examined in murine peritoneal macrophages (M phi s). H. capsulatum-containing M phi s exhibited a metabolite profile similar to that of zymosan-challenged phagocyte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2559-2564.1992
更新日期:1992-07-01 00:00:00
abstract::Male ICR Swiss mice (2 to 3 months old) were fed Candida albicans in their drinking water for 3 days, followed by no treatment, antibiotics in their drinking water (daily), or immunosuppressants given by intraperitoneal injection (two to three times weekly) over a 3- to 4-week period. The organs of animals were proces...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1558-1563.1987
更新日期:1987-07-01 00:00:00
abstract::Phagocytosis of unopsonized Salmonella typhimurium 395, MR-10, opsonized Salmonella typhimurium 395 MS, and Staphylococcus epidermidis by rabbit polymorphonuclear leukocytes was inhibited by heparin at concentrations as low as 0.5 U/ml. Inhibition was dose dependent and nearly complete at 20 U/ml. Provided that hepari...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.295-299.1981
更新日期:1981-04-01 00:00:00
abstract::Only listeriolysin O (LLO)-producing strains of Listeria monocytogenes generate protective immunity in mice. Based on the findings that endogenous gamma interferon (IFN-gamma) production was induced only by such strains and that purified LLO could induce IFN-gamma from NK cells, we have postulated that LLO may play a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.568-575.1999
更新日期:1999-02-01 00:00:00
abstract::A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.439-444.1977
更新日期:1977-05-01 00:00:00
abstract::Strain superinfection occurs when a second pathogen strain infects a host already infected with a primary strain. The selective pressures that drive strain divergence, which underlies superinfection, and allow penetration of a new strain into a host population are critical knowledge gaps relevant to shifts in infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02537-14
更新日期:2014-12-01 00:00:00
abstract::Gardnerella vaginalis, the bacterial species most frequently isolated from women with bacterial vaginosis (BV), produces a cholesterol-dependent cytolysin (CDC), vaginolysin (VLY). At sublytic concentrations, CDCs may initiate complex signaling cascades crucial to target cell survival. Using live-cell imaging, we obse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00627-13
更新日期:2013-12-01 00:00:00
abstract::The relationship between the selective abilities of bacteria to adhere and their predilections for colonizing different mammalian hosts was investigated by using bacteria indigenous to the tongue dorsum of humans and rats as models. Streptococcus salivarius and S. sanguis averaged 22.6 and 2.8%, respectively, of the c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.1.238-246.1976
更新日期:1976-01-01 00:00:00
abstract::The ability of various strains of rats to produce interferon in response to polyriboinosinic-polyribocytodilic acid was investigated. ACI and DA (RT-1a), BN (RT-1n), Buffalo (RT-1b), August (RT-1c), and GH (RT-1l) strains produced low levels of interferon in response to intraperitoneal administration of polyriboinosin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.580-583.1984
更新日期:1984-02-01 00:00:00
abstract::Species-specific antibody epitopes within several major immunoreactive protein orthologs of Ehrlichia species have recently been identified and molecularly characterized. In this study, dominant B-cell epitopes within the acidic (pI 5.35) ankyrin repeat-containing 200-kDa major immunoreactive protein (gp200) of Ehrlic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00041-07
更新日期:2007-10-01 00:00:00
abstract::Vero cytotoxin-producing Escherichia coli (VTEC) strains have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. Since adherence of enteric pathogens to epithelial surfaces is often a prerequisite for the subsequent delivery of bacterial enterotox...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
abstract::Both interleukin-17A (IL-17A) and IL-17F are proinflammatory cytokines that have an important role in intestinal homeostasis via receptor signaling. These cytokines have been characterized in chickens, but very little is known about their receptors and their functional activity. We provide here the first description o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02141-14
更新日期:2014-09-01 00:00:00
abstract::Toll-like receptor (TLR)-based signaling pathways in the host may be modulated by pathogens during the course of infection. We describe a novel immunomodulatory mechanism in which Aspergillus fumigatus conidia induce attenuation of TLR2- and TLR4-mediated interleukin (IL)-6 and IL-1beta proinflammatory responses in hu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01455-08
更新日期:2009-05-01 00:00:00
abstract::To study the immunological activity of proteins secreted by Mycobacterium tuberculosis, we carried out comparative studies in guinea pigs infected intravenously with 2.5 x 10(3) CFU of this organism or with 2.5 x 10(4) CFU of Mycobacterium bovis BCG. Groups of infected guinea pigs were skin tested with fractions of se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.804-810.1995
更新日期:1995-03-01 00:00:00
abstract::Thymidine kinase (TK) from herpes simplex virus type 1 (HSV-1) biochemically transformed HeLa cells, purified by affinity chromatography, has been characterized with respect to its electrophoretic mobility, molecular weight, activation energy, substrate specificity, and immunological specificity. TK purified from HSV-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.486-492.1977
更新日期:1977-05-01 00:00:00
abstract::Akabane virus (a Bunyavirus) has been associated with epizootics of congenital deformities in cattle, sheep, and goats. Experimental studies using mouse-adapted virus inoculated intravenously into pregnant sheep gave an inapparent infection. Neutralizing antibodies were detected on day 5, and peaks in the titer were s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.254-262.1977
更新日期:1977-01-01 00:00:00
abstract::An "immunogenic" ribonucleic acid (Im-RNA) has been extracted from peritoneal exudate (PE) cells of rats that were immunized with sheep erythrocytes (SRBC). Following multiple phenol extractions and deoxyribonuclease treatment, the material obtained from PE cells was eluted from diethylaminoethyl-cellulose at 0.55 M N...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.2.215-221.1973
更新日期:1973-08-01 00:00:00
abstract::By using the powerful separation technique of capillary gas chromatography combined with the selectivity of mass spectrometric detection, muramic acid was not detectable in purified elementary bodies of Chlamydia psittaci Cal 10 (less than or equal to 0.006%) or C. trachomatis serovar E (less than or equal to 0.02%). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.835-837.1990
更新日期:1990-03-01 00:00:00