Abstract:
:Salmonella pathogenicity island 12 (SPI-12) of Salmonella enterica serovar Typhimurium is a 15-kb region that encompasses genes STM2230 to STM2245 and encodes a remnant phage known to contribute to bacterial virulence. In mouse infection experiments and replication assays in macrophages, we demonstrated a role for four genes in SPI-12 for bacterial survival in the host. STM2239, a potential Q antiterminator, showed a prominent contribution to bacterial fitness. Transcriptional reporter experiments, quantitative reverse transcription-PCR (RT-PCR), and immunoblotting demonstrated that the virulence regulator SsrB and STM2239 contribute to transcriptional activation of genes in SPI-12. SsrB was found to indirectly regulate this locus by transcriptional read-through from the sspH2 (STM2241) promoter. Chromatin immunoprecipitation showed that STM2239 copurified with the promoter regulating STM2237, suggesting that STM2239 may function as an antiterminator to activate adjacent genes. These results demonstrate that bacteriophage genes may be adapted by pathogenic bacteria to improve fitness in the host.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tomljenovic-Berube AM,Henriksbo B,Porwollik S,Cooper CA,Tuinema BR,McClelland M,Coombes BKdoi
10.1128/IAI.00067-13subject
Has Abstractpub_date
2013-07-01 00:00:00pages
2394-404issue
7eissn
0019-9567issn
1098-5522pii
IAI.00067-13journal_volume
81pub_type
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