Abstract:
:The efficacy of the innate immune system depends on its ability to mount an appropriate response to diverse infections and damaging agents. Key components of this system are pattern recognition receptors that detect pathogen-associated and damage-associated molecular patterns (PAMPs and DAMPs). Nlrp1b is a pattern recognition receptor that forms a caspase-1 activation platform, known as an inflammasome, upon sensing the proteolytic activity of anthrax lethal toxin. The activation of caspase-1 leads to the release of proinflammatory cytokines that aid in the clearance of the anthrax infection. Here, we demonstrate that Nlrp1b also becomes activated in cells that are subjected to energy stress caused by metabolic inhibitors or by nutrient deprivation. Glucose starvation and hypoxia were used to correlate the level of cytosolic ATP to the degree of inflammasome activation. Because lowering the ratio of cytosolic ATP to AMP activates the main cellular energy sensor, AMP-activated protein kinase (AMPK), we assessed whether AMPK promoted inflammasome activity by using a combination of small interfering RNA (siRNA) and transfection of a dominant negative AMPK subunit. We found that AMPK promoted inflammasome activity, but activation of AMPK in the absence of ATP depletion was not sufficient for caspase-1-mediated pro-interleukin 1β (pro-IL-1β) processing. Finally, we found that mutation of the ATP-binding motif of Nlrp1b caused constitutive activation, suggesting that ATP might inhibit the Nlrp1b inflammasome instead of being required for its assembly.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Liao KC,Mogridge Jdoi
10.1128/IAI.01003-12subject
Has Abstractpub_date
2013-02-01 00:00:00pages
570-9issue
2eissn
0019-9567issn
1098-5522pii
IAI.01003-12journal_volume
81pub_type
杂志文章abstract::An "immunogenic" ribonucleic acid (Im-RNA) has been extracted from peritoneal exudate (PE) cells of rats that were immunized with sheep erythrocytes (SRBC). Following multiple phenol extractions and deoxyribonuclease treatment, the material obtained from PE cells was eluted from diethylaminoethyl-cellulose at 0.55 M N...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.2.215-221.1973
更新日期:1973-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01369-07
更新日期:2008-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
更新日期:1982-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05904-11
更新日期:2012-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3720-3726.1989
更新日期:1989-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2718-2722.2001
更新日期:2001-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.332-342.1996
更新日期:1996-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00407-08
更新日期:2008-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.92-94.1974
更新日期:1974-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1821-1831.2001
更新日期:2001-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.5.642-647.1971
更新日期:1971-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.28-32.1983
更新日期:1983-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3155-3163.1998
更新日期:1998-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.583-588.1978
更新日期:1978-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01020-10
更新日期:2011-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00
abstract::Kinetoplastid membrane protein 11 (KMP-11) from Leishmania donovani is an abundant 11-kDa surface membrane glycoprotein. Lymph node cells from mice of six different H-2 haplotypes immunized with KMP-11 or with L. donovani promastigotes were stimulated to proliferate in vitro KMP-11. Primed purified T cells required an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4893-4899.1994
更新日期:1994-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.774-781.2004
更新日期:2004-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.753-757.1987
更新日期:1987-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2010-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.3.553-559.1986
更新日期:1986-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3799-3806.1992
更新日期:1992-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.14-22.1975
更新日期:1975-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.1082-1090.1993
更新日期:1993-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2688-2693.1992
更新日期:1992-07-01 00:00:00
abstract::As an intracellular protozoan parasite, Toxoplasma gondii is likely to exploit proteases for host cell invasion, acquisition of nutrients, avoidance of host protective responses, escape from the parasitophorous vacuole, differentiation, and other activities. T. gondii serine protease inhibitor 1 (TgPI1) is the most ab...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06167-11
更新日期:2012-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.757-765.1981
更新日期:1981-12-01 00:00:00
abstract::Immunoglobulin class-specific antibodies were measured by a solid-phase radioimmunoassay in serum, bronchoalveolar washings (BAW), lung cell lysates, and spleen cell lysates in germfree mice after intranasal (i.n.) and intraperitoneal (i.p.) primary and secondary 10(5), 10(4), and 10(3) mean tissue culture infective d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.3.521-527.1977
更新日期:1977-09-01 00:00:00
abstract::A murine immunoglobulin G monoclonal antibody (MAb) raised against outer membrane vesicles of Moraxella catarrhalis 035E was shown to bind to a surface-exposed epitope of a major outer membrane protein of this organism. This outer membrane protein, which had an apparent molecular weight of approximately 80,000 in sodi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2003-2010.1993
更新日期:1993-05-01 00:00:00
abstract::Borrelia burgdorferi undergoes differential gene expression during transmission from its tick vector to a vertebrate host. The addition of blood to a spirochete culture at 35 degrees C for 48 h had a dramatic effect on gene expression of this organism. Utilizing B. burgdorferi whole genome DNA arrays, we compared the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5419-5432.2004
更新日期:2004-09-01 00:00:00