Abstract:
:We studied seven strains of group G streptococci isolated from clinically severe bacteremic infections in six intravenous drug abusers. These group G strains multiplied luxuriantly in fresh human blood. On electron microscopy, they exhibited surface fibrillae similar to those observed in M-protein-rich group A streptococci, but they were not serologically M typable with a battery of 39 M antisera. Rabbit antisera raised against two of the group G strains (1618 and 1750) opsonized the homologous but not the heterologous isolates and exhibited type-specific Ouchterlony immunoprecipitin reactions. Moreover, antisera raised against peptic extracts of strain 1750 also promoted phagocytic killing of that strain. Anti-1750 reacted in high titer in an enzyme-linked immunosorbent assay against peptic extracts of the homologous strain; these antibodies were removed by absorption with 1750 cells but not by absorption with heterologous strains. These studies represent, to our knowledge, the first analysis of virulence factors of group G streptococci isolated from invasive human disease. The seven epidemiologically related blood isolates of group G streptococci possess distinct type-specific, antiphagocytic surface virulence factors analogous to the M proteins of group A streptococci.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Bisno AL,Craven DE,McCabe WRdoi
10.1128/IAI.55.3.753-757.1987subject
Has Abstractpub_date
1987-03-01 00:00:00pages
753-7issue
3eissn
0019-9567issn
1098-5522journal_volume
55pub_type
杂志文章abstract::Knowledge of whether Mycobacterium tuberculosis resides within a relatively impermeable membrane-bound vacuole or is free within the cytoplasm within its host cell is central to an understanding of the immunobiology of this intracellular parasite but is a matter of controversy. To explore this issue, we assessed the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5800-5807.2002
更新日期:2002-10-01 00:00:00
abstract::We compared a cryptococcal culture filtrate antigen referred to as CneF with chemically defined cryptococcal antigen fractions isolated by Cherniak and co-workers by using double immunodiffusion gels, polyacrylamide gel electrophoresis, immunoblots, and footpad reactivity of immunized mice. The three previously descri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.2.424-431.1988
更新日期:1988-02-01 00:00:00
abstract::Vibrio cholerae neuraminidase (NANase) is hypothesized to act synergistically with cholera toxin (CT) and increase the severity of a secretory response by increasing the binding and penetration of CT to enterocytes. To test this hypothesis, the NANase gene (nanH) from V. cholerae Ogawa 395 was first cloned and sequenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.406-415.1992
更新日期:1992-02-01 00:00:00
abstract::Whole blood and plasma from animals in the acute stage of leptospirosis contained a toxic factor which produced a cytopathic effect on fibroblastic L cell monolayers. Firm adsorption of cytotoxic factor (CTF) to L cells occurred within 1 h. The highest titer of CTF in plasma was reached at 24 h and declined after 48 h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.401-405.1973
更新日期:1973-09-01 00:00:00
abstract::Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.158-160.1978
更新日期:1978-04-01 00:00:00
abstract::Strains of type III group B streptococci isolated from patients with neonatal sepsis are generally resistant to complement-mediated phagocytic killing in the absence of specific antibody. It has been suggested that the resistance of type III group B streptococci to phagocytosis results from inhibition of alternative-c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.3986-3993.1992
更新日期:1992-10-01 00:00:00
abstract::We studied interleukin-1 (IL-1) secretion by rat peritoneal exudate macrophages stimulated with purified toxic shock syndrome toxin-1 (TSST-1). TSST-1 was observed to be a more potent inducer of IL-1 than was endotoxin. The induction of IL-1 secretion by TSST-1 was not blocked by polymyxin B but could be blocked by mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.2865-2869.1987
更新日期:1987-12-01 00:00:00
abstract::Analysis of major outer membrane protein (MOMP) profiles of various meningococci by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of 0 to 2 low-molecular-weight, heat-modifiable MOMPs (molecular weight, 25,000 to 32,000) and 1 to 3 high-molecular-weight MOMPs (molecul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Clostridium perfringens type B and type C isolates, which produce beta-toxin (CPB), cause fatal diseases originating in the intestines of humans or livestock. Our previous studies demonstrated that CPB is necessary for type C isolate CN3685 to cause bloody necrotic enteritis in a rabbit ileal loop model and also showe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00547-08
更新日期:2008-10-01 00:00:00
abstract::Anopheles gambiae is the major African vector of Plasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00864-06
更新日期:2007-04-01 00:00:00
abstract::The hallmarks of pulmonary Mycobacterium tuberculosis infection are lung granulomas. These organized structures are composed of host immune cells whose purpose is to contain or clear infection, creating a complex hub of immune and bacterial cell activity, as well as limiting pathology in the lungs. Yet, given cellular...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00426-18
更新日期:2018-08-22 00:00:00
abstract::The outer membrane of pathogenic Leptospira species grown in culture media contains lipopolysaccharide (LPS), a porin (OmpL1), and several lipoproteins, including LipL36 and LipL41. The purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. Ha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.853-861.1999
更新日期:1999-02-01 00:00:00
abstract::Inbred CBA/J mice were used in developing a defined in vivo model for studying host-parasite relationships in cryptococcosis. Mice were infected either intranasally or intraperitoneally with 10(3) viable Cryptococcus neoformans cells. At weekly intervals over a 92-day period, C. neoformans growth profiles in the lungs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::A novel in vivo expression technology (IVET) was performed to identify Klebsiella pneumoniae CG43 genes that are specifically expressed during infection of BALB/c mice. The IVET employed a UDP glucose pyrophosphorylase (galU)-deficient mutant of K. pneumoniae which is incapable of utilizing galactose and synthesizing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.7140-7145.2001
更新日期:2001-11-01 00:00:00
abstract::The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.314-319.1984
更新日期:1984-08-01 00:00:00
abstract::A model of invasive Haemophilus influenzae type b disease in rats was used to compare the net in vivo and in vitro multiplication rates of this bacterium. In both the blood of rats and in vitro cultures (fresh rat blood or enriched broth) there was an exponential increase in the number of CFU per milliliter with calcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.911-913.1986
更新日期:1986-06-01 00:00:00
abstract::Once in the cytoplasm of mammalian cells, Shigella flexneri expresses a motile phenotype caused by polar directional assembly of actin. This process depends on accumulation of IcsA (VirG), a 120-kDa protein with ATPase activity, at the pole of the bacterium opposite to that at which ongoing septation occurs. IcsA is a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.511-517.1996
更新日期:1996-02-01 00:00:00
abstract::In nature, mixed Borrelia burgdorferi infections are common and possibly can be acquired by either superinfection or coinfection. Superinfection by heterologous B. burgdorferi strains has been established experimentally, although the ability of homologous B. burgdorferi clones to superinfect a host has not been studie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01817-14
更新日期:2014-11-01 00:00:00
abstract::Aerobic microorganisms were isolated and identified from 9 of 10 malignant tissues aseptically obtained from surgical patients. The organisms isolated are species commonly associated with the flora of the human body. When these cancer-associated organisms were grown in Trypticase soy broth (BBL Microbiology Systems), ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1211-1215.1981
更新日期:1981-06-01 00:00:00
abstract::The susceptibility of a few strains of mice to a subcutaneous injection of Leishmania tropica major, the causative agent of cutaneous leishmaniasis in humans, was studied. The infection in six strains (CBA, AKR/J, AKR/cu, C57BL/6, A/J, and C3H) remained cutaneous, and the animals recovered within 3 to 4 months. In con...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.611-614.1979
更新日期:1979-11-01 00:00:00
abstract::Relapsing fever (RF) is a spirochetal infection characterized by periods of sickness with fever at time of high bacteremia that alternate with afebrile periods of relative well being during low bacteremia. Patients with epidemic RF who are doing relatively well have extraordinarily high levels of interleukin-10 (IL-10...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00587-08
更新日期:2008-12-01 00:00:00
abstract::Twitching motility is a form of surface-associated bacterial movement mediated by type IV pili of Pseudomonas aeruginosa. Others have shown that pilT and pilU mutants, which are piliated but defective in twitching motility, display reduced cytotoxic capacity towards epithelial cells in vitro. Although these mutants ef...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5389-5393.2003
更新日期:2003-09-01 00:00:00
abstract::Four classes of mutants of type III group B streptococcus were isolated by serial subculture of the wild-type strain in the presence of type III-specific rabbit antiserum. Class I mutants no longer synthesized sialic acid but still elaborated the core antigen. Class II mutants maintained the ability to synthesize sial...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.141-151.1983
更新日期:1983-10-01 00:00:00
abstract::Legionella pneumophila, the gram-negative agent of Legionnaires' disease, possesses type IV pili and a type II protein secretion (Lsp) system, both of which are dependent upon the PilD prepilin peptidase. By analyzing multiple pilD mutants and various types of Lsp mutants as well as performing trans-complementation of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.310-321.2004
更新日期:2004-01-01 00:00:00
abstract::Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.220-226.1974
更新日期:1974-07-01 00:00:00
abstract::During an etiological study of Kawasaki disease (mucocutaneous lymph node syndrome [MCLS]), we found that dominant viridans streptococcal strains on tooth surfaces and in the throat of both MCLS patients and non-MCLS control children formed erythrogenic and biologically active, extracellular products. In this study, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5252-5260.1993
更新日期:1993-12-01 00:00:00
abstract::Pseudomonas aeruginosa invades various epithelial cell types in vitro and in vivo. The P. aeruginosa genome possesses a gene (flhA) which encodes a protein that is believed to be part of the export apparatus for flagellum assembly and which is homologous to invA of Salmonella spp. Because invA is required for invasion...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.4931-4937.2001
更新日期:2001-08-01 00:00:00
abstract::The lipid A portion has been identified as the active center responsible for lipopolysaccharide (LPS)-induced macrophage activation. However, we found that Salmonella (Salmonella enterica serovars Abortusequi, Minnesota, and Typhimurium) lipid A is inactive in human macrophages, despite its LPS being highly active. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6043-6047.2002
更新日期:2002-11-01 00:00:00
abstract::Natural-resistance-associated macrophage protein 1 (Nramp1) is a divalent cation transporter belonging to a family of transporter proteins highly conserved in eukaryotes and prokaryotes. Mammalian and bacterial transporters may compete for essential metal ions during mycobacterial infections. The mycobacterial Nramp h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4124-4131.2002
更新日期:2002-08-01 00:00:00
abstract::Although there is substantial evidence that type IV pili purified from diarrhea-associated Aeromonas species (designated Bfp for bundle-forming pilus) are intestinal colonization factors (S. M. Kirov, L. A. O'Donovan, and K. Sanderson, Infect. Immun. 67:5447-5454, 1999), nothing is known regarding the function of a se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4040-4048.2000
更新日期:2000-07-01 00:00:00