Abstract:
:Although there is substantial evidence that type IV pili purified from diarrhea-associated Aeromonas species (designated Bfp for bundle-forming pilus) are intestinal colonization factors (S. M. Kirov, L. A. O'Donovan, and K. Sanderson, Infect. Immun. 67:5447-5454, 1999), nothing is known regarding the function of a second family of Aeromonas type IV pili (designated Tap for type IV Aeromonas pilus), identified following the cloning of a pilus biogenesis gene cluster tapABCD. Related pilus gene clusters are widely conserved among gram-negative bacteria, but their significance for virulence has been controversial. To investigate the role of Tap pili in Aeromonas pathogenesis, mutants of Aeromonas strains (a fish isolate of A. hydrophila and a human dysenteric isolate of A. veronii bv. sobria) were prepared by insertional inactivation of the tapA gene which encodes the type IV pilus subunit protein, TapA. Exotoxic activities were unaffected by the mutation in tapA. Inactivation of tapA had no effect on the bacterial adherence of these two isolates to HEp-2 cells. For the A. veronii bv. sobria isolate, adhesion to Henle 407 intestinal cells and to human intestinal tissue was also unaffected. There was no significant effect on the duration of colonization or incidence of diarrhea when the A. veronii bv. sobria strain was tested in the removable intestinal tie adult rabbit diarrhea model or on its ability to colonize infant mice. Evidence was obtained that demonstrated that TapA was expressed by both Aeromonas species and was present on the cell surface, although if assembled into pili this pilus type appears to be an uncommon one under standard bacterial growth conditions. Further studies into factors which may influence Tap expression are required, but the present study suggests that Tap pili may not be as significant as Bfp pili for Aeromonas intestinal colonization.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kirov SM,Barnett TC,Pepe CM,Strom MS,Albert MJdoi
10.1128/iai.68.7.4040-4048.2000keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
4040-8issue
7eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::A superoxide dismutase (SOD) gene from the obligate intracellular bacterium Coxiella burnetii has been cloned, and its DNA sequence has been determined and expressed in Escherichia coli. The gene was identified on pSJR50, a pHC79-derived genomic clone, by using the polymerase chain reaction with degenerate oligonucleo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3814-3823.1992
更新日期:1992-09-01 00:00:00
abstract::Staphylococcus aureus is one of the pathogens most frequently isolated in device-related infections. S. aureus is equipped with surface-associated proteins promoting specific binding to matrix molecules. Clumping factor A (ClfA, encoded by clfA) mediates adhesion to fibrinogen. Whereas the contribution of ClfA to path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2758-2762.2002
更新日期:2002-06-01 00:00:00
abstract::Repeated immunizations of CF1 mice with irradiated noninfectious Trypanosoma cruzi trypomastigotes resulted in partial protection against infection with live parasites. It also induced a limited number of antibody species that were reactive in Western blots with trypomastigote but not with epimastigote or amastigote p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.826-830.1984
更新日期:1984-12-01 00:00:00
abstract::Vibrio vulnificus is an oyster-associated bacterial pathogen that causes life-threatening fulminating septicemia and necrotizing wound infections in humans. The capsular polysaccharide of V. vulnificus (VvPS) is critical for virulence. Previously we showed that active immunization of mice with a VvPS-tetanus toxoid (V...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2220-2224.1996
更新日期:1996-06-01 00:00:00
abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00
abstract::Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The consequences of vaccination were evaluated in terms of the growth of BCG in the lungs and spleen, lung and spleen weigh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Neutralizing antibodies were raised in mice that had been inoculated repeatedly with moderate quantities of human leukocyte interferon highly purified by affinity chromatography on immobilized anti-interferon globulins. Interferon preparations of lesser purity sensitized the mice to subsequent inoculations of interfer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.570-574.1978
更新日期:1978-02-01 00:00:00
abstract::Cell walls from Streptococcus mutans were prepared by conventional technique and subjected to a series of extraction procedures involving classical protein solvents. The extracted walls contained several non-peptidoglycan amino acids and were also amenable to radiolabeling with [125I]sodium iodide and chloramine T. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.118-126.1980
更新日期:1980-04-01 00:00:00
abstract::Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4883-4889.1995
更新日期:1995-12-01 00:00:00
abstract::Despite the autotransporter (AT) moniker, AT secretion appears to involve the function of periplasmic chaperones. We identified four periplasmic proteins that specifically bound to plasmid-encoded toxin (Pet), an AT produced by enteroaggregative Escherichia coli (EAEC). These proteins include the 17-kDa Skp chaperone ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00167-12
更新日期:2012-07-01 00:00:00
abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2591-2597.2002
更新日期:2002-05-01 00:00:00
abstract::The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6744-6749.2000
更新日期:2000-12-01 00:00:00
abstract::In the development of vaccines capable of providing immunity against brucellosis, Cu-Zn superoxide dismutase (SOD) has been demonstrated to be one of the protective immunogens of Brucella abortus. In an earlier study, we provided strong evidence that intramuscular injection with a plasmid DNA carrying the SOD gene (pc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2081-2087.2004
更新日期:2004-04-01 00:00:00
abstract::Erysipelothrix rhusiopathiae is a primary pathogen of swine and turkeys and sporadic cause of disease in a variety of other hosts, including humans. A genomic library of the highly virulent strain of E. rhusiopathiae E1-6P was constructed in the expression-cloning vector lambda gt11 and screened with serum from a pig ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.3116-3121.1990
更新日期:1990-09-01 00:00:00
abstract::We previously identified a cell wall-associated protein from Fusobacterium nucleatum, a Gram-negative bacterium of the oral cavity, that induces human beta defensin 2 (hBD-2) in primary human oral epithelial cells (HOECs) and designated it FAD-I (Fusobacterium-associated defensin inducer). Here, we report differential...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01311-15
更新日期:2016-04-22 00:00:00
abstract::Previous studies have demonstrated that animals exposed to Streptococcus pneumoniae while recovering from influenza A virus infection exhibit exacerbated disease symptoms. However, many of the current animal models exploring dual viral and bacterial synergistic exacerbations of respiratory disease have utilized mouse-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01943-06
更新日期:2007-06-01 00:00:00
abstract::Malaria infection treatment vaccine (ITV) is a promising strategy to induce homologous and heterologous protective immunity against the blood stage of the parasite. However, the underlying mechanism of protection remains largely unknown. Here, we found that a malaria-specific antibody (Ab) could mediate the protective...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02621-14
更新日期:2015-05-01 00:00:00
abstract::In an attempt to clarify the mechanism(s) of increased susceptibility to oral infection in diabetics, we examined the levels of salivary antibacterial factors, including lysozyme, lactoperoxidase, and lactoferrin, in diabetic hamsters whose condition was induced with streptozotocin. Saliva was collected from these ham...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.2.389-394.1985
更新日期:1985-05-01 00:00:00
abstract::Genetic diversity and relationships among 109 isolates of Neisseria meningitidis obtained from throat cultures of healthy individuals in Norway in 1984 were assessed by analyzing electrophoretically demonstrable allelic variation at 15 enzyme-encoding chromosomal genes. Seventy-eight distinctive electrophoretic types ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2060-2068.1988
更新日期:1988-08-01 00:00:00
abstract::Assembly of adherent biofilms is the key mechanism involved in Staphylococcus epidermidis virulence during device-associated infections. Aside from polysaccharide intercellular adhesin (PIA), the accumulation-associated protein Aap and the extracellular matrix binding protein Embp act as intercellular adhesins, mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01142-10
更新日期:2011-06-01 00:00:00
abstract::In the absence of antiserum, rabbit polymorphonuclear leukocytes (PMNs) released lysosomal enzymes in response to Actinomyces viscosus (19246) but not to Streptococcus mutans (6715). Antibodies had a marked modulating influence on these reactions. PMN hydrolase release was significantly enhanced to both organisms when...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.6.1315-1321.1976
更新日期:1976-12-01 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease in cattle. The complex, multifaceted interaction of M. avium subsp. paratuberculosis with its host includes dampening the ability of infected cells to respond to stimuli that promote M. avium subsp. paratuberculosis clearance. By dis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00785-12
更新日期:2013-01-01 00:00:00
abstract::The ScpC protease of Streptococcus pyogenes degrades interleukin-8 (IL-8), a chemokine that mediates neutrophil transmigration and activation. The ability to degrade IL-8 differs dramatically among clinical isolates of S. pyogenes. Bacteria expressing ScpC overcome immune clearance by preventing the recruitment of neu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00128-08
更新日期:2008-09-01 00:00:00
abstract::In bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4005-4014.1994
更新日期:1994-09-01 00:00:00
abstract::The possibility that soluble products of Schistosoma mansoni eggs might participate in the pathogenesis of hepatic fibrosis in schistosomiasis was investigated. Both crude saline extracts of eggs (soluble egg antigen [SEA]) and a partially purified SEA fraction contained activity which stimulated guinea pig and human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.103-108.1982
更新日期:1982-10-01 00:00:00
abstract::Pneumonic plague, caused by inhalation of Yersinia pestis, represents a major bioterrorism threat for which no vaccine is available. Based on the knowledge that genetic delivery of monoclonal antibodies (MAbs) with adenovirus (Ad) gene transfer vectors results in rapid, high-level antibody expression, we evaluated the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00856-08
更新日期:2009-04-01 00:00:00
abstract::In previous studies, we reported that fibronectin (FN) markedly enhances phagocytic uptake of antibody-coated group B streptococci (GBS) by human polymorphonuclear leukocytes. Furthermore, administration of FN along with a GBS type-specific monoclonal or polyclonal antibody to infected neonatal rats significantly enha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2334-2339.1993
更新日期:1993-06-01 00:00:00
abstract::Host control of Coxiella burnetii infections is believed to be mediated primarily by activated monocytes/macrophages. The activation of macrophages by cytokines leads to the production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) that have potent antimicrobial activities. The contri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6666-6675.2004
更新日期:2004-11-01 00:00:00
abstract::Cholera toxin (CT) and the type II heat-labile enterotoxins (HLT) LT-IIa and LT-IIb act as potent systemic and mucosal adjuvants and induce distinct T-helper (Th)-cell cytokine profiles. In the present study, CT and the type II HLT were found to differentially affect cytokine production by anti-CD3-stimulated human pe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4486-4492.2001
更新日期:2001-07-01 00:00:00
abstract::A significant depression in the phagocytic capacity of elicited peritoneal macrophages, pulmonary alveolar macrophages, and elicited peritoneal polymorphonucleated neutrophils was manifested when the cells were incubated in medium containing cadmium chloride. With the exception of the neutrophils, a similar influence ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.378-381.1978
更新日期:1978-11-01 00:00:00