Abstract:
:We compared a cryptococcal culture filtrate antigen referred to as CneF with chemically defined cryptococcal antigen fractions isolated by Cherniak and co-workers by using double immunodiffusion gels, polyacrylamide gel electrophoresis, immunoblots, and footpad reactivity of immunized mice. The three previously described components of cryptococcal culture filtrates are a high-molecular-weight glucuronoxylo-mannan (GXM), which is the major constituent, a galactoxylomannan (GaIXM), and a mannoprotein (MP). In this study we demonstrated that CneF contained components which were serologically and electrophoretically similar to the three previously described cryptococcal culture filtrate fractions. The MP fraction elicited significantly stronger delayed-type hypersensitivity responses than did the GXM or GaIXM fraction when used in mice immunized either with the CneF in complete Freund adjuvant or whole heat-killed Cryptococcus neoformans yeast cells. These findings were confirmed when the footpads of immunized mice were challenged with GaIXM and MP preparations from a culture filtrate of a C. neoformans acapsular mutant that does not produce GXM. Thus, we concluded that the MP was the primary component recognized by the anticryptococcal cell-mediated immune response in mice.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Murphy JW,Mosley RL,Cherniak R,Reyes GH,Kozel TR,Reiss Edoi
10.1128/IAI.56.2.424-431.1988subject
Has Abstractpub_date
1988-02-01 00:00:00pages
424-31issue
2eissn
0019-9567issn
1098-5522journal_volume
56pub_type
杂志文章abstract::Leishmania-infected macrophages are potential antigen-presenting cells for CD4+ T lymphocytes, which recognize parasite antigens bound to major histocompatibility complex class II molecules (Ia). However, the intracellular sites where Ia and antigens may interact are far from clear, since parasites grow within the mod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.764-775.1991
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journal_title:Infection and immunity
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doi:10.1128/IAI.14.1.163-167.1976
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journal_title:Infection and immunity
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doi:10.1128/IAI.19.2.457-461.1978
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.701-708.1985
更新日期:1985-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.236-239.1974
更新日期:1974-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.269-271.1990
更新日期:1990-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2016-07-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.158-160.1978
更新日期:1978-04-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1984-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.00711-10
更新日期:2010-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6148-6154.2003
更新日期:2003-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1897-1900.1988
更新日期:1988-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.897-898.1986
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journal_title:Infection and immunity
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更新日期:2005-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2020-07-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4818-4824.1994
更新日期:1994-11-01 00:00:00
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更新日期:2002-06-01 00:00:00