Scrapie in vitro: agent replication and reduced cell yield.

Abstract:

:Exposure of PAM cells, a spontaneously transformed mouse cell line, to brain homogenates from mice infected with scrapie caused a relative decrease in total cell yield, which persisted from passage 2 or 3 to passage 18 after treatment. The effect was elicited by each of the eight independent scrapie isolates tested. Lysates prepared from cultures 16 passages after treatment with scrapie caused the decrease when applied to fresh PAM cultures. Mice inoculated with passage 14 and 18 lysates developed a reduced percentage of polymorphonuclear neutrophils by 5 weeks and scrapie disease by 6 to 9 months after inoculation. Based on the total dilution from treatment of the PAM cultures with scrapie material to the preparation of the lysates, we conclude that the agent(s) responsible for the reduced PAM cell yield, the decreased percentage of polymorphonuclear neutrophils, and the induction of scrapie disease had replicated in the PAM cells. By filtration, the diameter of the agent causing the reduction in cell yield was estimated to be between 25 and 50 nm.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Carp RI,Merz GS,Licursi PC

doi

10.1128/IAI.14.1.163-167.1976

subject

Has Abstract

pub_date

1976-07-01 00:00:00

pages

163-7

issue

1

eissn

0019-9567

issn

1098-5522

journal_volume

14

pub_type

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