Abstract:
:Catheter-associated urinary tract infections (CAUTIs) constitute the majority of nosocomial UTIs and pose significant clinical challenges. Enterococcal species are among the predominant causative agents of CAUTIs. However, very little is known about the pathophysiology of Enterococcus-mediated UTIs. We optimized a murine model of foreign body-associated UTI in order to mimic conditions of indwelling catheters in patients. In this model, the presence of a foreign body elicits major histological changes and induces the expression of several proinflammatory cytokines in the bladder. In addition, in contrast to naïve mice, infection of catheter-implanted mice with Enterococcus faecalis induced the specific expression of interleukin 1β (IL-1β) and macrophage inflammatory protein 1α (MIP-1α) in the bladder. These responses resulted in a favorable niche for the development of persistent E. faecalis infections in the murine bladders and kidneys. Furthermore, biofilm formation on the catheter implant in vivo correlated with persistent infections. However, the enterococcal autolytic factors GelE and Atn (also known as AtlA), which are important in biofilm formation in vitro, are dispensable in vivo. In contrast, the housekeeping sortase A (SrtA) is critical for biofilm formation and virulence in CAUTIs. Overall, this murine model represents a significant advance in the understanding of CAUTIs and underscores the importance of urinary catheterization during E. faecalis uropathogenesis. This model is also a valuable tool for the identification of virulence determinants that can serve as potential antimicrobial targets for the treatment of enterococcal infections.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Guiton PS,Hung CS,Hancock LE,Caparon MG,Hultgren SJdoi
10.1128/IAI.00711-10subject
Has Abstractpub_date
2010-10-01 00:00:00pages
4166-75issue
10eissn
0019-9567issn
1098-5522pii
IAI.00711-10journal_volume
78pub_type
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abstract::After oral feeding of mice with avirulent Salmonella, Escherichia coli, or hybrid strains, only certain bacterial strains were able to multiply and persist within the small intestinal Peyer's patches. After oral vaccination alone, or oral priming and subsequent parenteral boosting, antibody class and titers were detec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.27-33.1979
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1081-1085.1983
更新日期:1983-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1983-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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更新日期:2015-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.15-18.1979
更新日期:1979-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.887-892.1990
更新日期:1990-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.838-843.1979
更新日期:1979-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00050-20
更新日期:2020-04-20 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1992-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Infection and immunity
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更新日期:2015-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.378-381.1977
更新日期:1977-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.3034-3035.2000
更新日期:2000-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1383-1390.1974
更新日期:1974-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.798-803.1980
更新日期:1980-03-01 00:00:00
abstract::To elucidate the mechanism of the high incidence of lower respiratory tract infections in patients with diabetes mellitus, we investigated the kinetics of production of macrophage inflammatory protein 2 (MIP-2), an important mediator of lung neutrophil recruitment, using mice with streptozotocin-induced diabetes. Intr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2925-2929.2000
更新日期:2000-05-01 00:00:00