Abstract:
:We report here the identification and characterization of two zinc uptake systems, ZurAM and ZinABC, in the intracellular pathogen Listeria monocytogenes. Transcription of both operons was zinc responsive and regulated by the zinc-sensing repressor Zur. Deletion of either zurAM or zinA had no detectable effect on growth in defined media, but a double zurAM zinA mutant was unable to grow in the absence of zinc supplementation. Deletion of zinA had no detectable effect on intracellular growth in HeLa epithelial cells. In contrast, growth of the zurAM mutant was significantly impaired in these cells, indicating the importance of the ZurAM system during intracellular growth. Notably, the deletion of both zinA and zurAM severely attenuated intracellular growth, with the double mutant being defective in actin-based motility and unable to spread from cell to cell. Deletion of either zurAM or zinA had a significant effect on virulence in an oral mouse model, indicating that both zinc uptake systems are important in vivo and establishing the importance of zinc acquisition during infection by L. monocytogenes. The presence of two zinc uptake systems may offer a mechanism by which L. monocytogenes can respond to zinc deficiency within a variety of environments and during different stages of infection, with each system making distinct contributions under different stress conditions.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Corbett D,Wang J,Schuler S,Lopez-Castejon G,Glenn S,Brough D,Andrew PW,Cavet JS,Roberts ISdoi
10.1128/IAI.05904-11subject
Has Abstractpub_date
2012-01-01 00:00:00pages
14-21issue
1eissn
0019-9567issn
1098-5522pii
IAI.05904-11journal_volume
80pub_type
杂志文章abstract::When rabbit peritoneal exudate cells were incubated for 24 and 48 h with phytohemagglutinin-activated lymphocytes or their culture supernatants, two times as many cells remained adherent to culture slides as in the controls. More spreading cells were found among the adherent cells in the stimulated cultures. Eighty pe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.5.1442-1448.1976
更新日期:1976-05-01 00:00:00
abstract::Recent data suggest that fibronectin may favor Staphylococcus aureus infection by promoting attachment to either injured tissues or implanted foreign bodies. We studied the quantitative adsorption of fibronectin onto polymethylmethacrylate (PMMA) cover slips by using a 125I-labeled preparation of the purified plasma g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.768-774.1984
更新日期:1984-09-01 00:00:00
abstract::Murine monoclonal antibodies were generated against streptolysin O. One out of 10 tested immunoglobulin clones exhibited strong neutralizing activity; in solution, the presence of approximately two to four antibody molecules per toxin monomer effected 50% neutralization of hemolytic toxin activity. An enzyme-linked im...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.641-645.1986
更新日期:1986-12-01 00:00:00
abstract::Comparative studies on the virulence of two strains of Pseudomonas aeruginosa isolated from otic pus and mink pneumonic lesion, respectively, were made by means of intranasal inoculation in mink. The minimal lethal dose of the former (strain NC-5, highly virulent for mice) was 10(10), and that of the latter (strain No...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.16-20.1974
更新日期:1974-07-01 00:00:00
abstract::Borrelia burgdorferi, a tick-borne bacterial pathogen, causes a disseminated infection involving multiple organs known as Lyme disease. Surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interaction with host factors. We show here that a fraction of the B. burgd...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05671-11
更新日期:2012-01-01 00:00:00
abstract::We tested the hypothesis that the intensity of specific antichlamydial T cell-mediated immunity in the genital tract of female guinea pigs infected intravaginally with the chlamydial agent of guinea pig inclusion conjunctivitis would determine the resistance or susceptibility to reinfection after a primary chlamydial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1346-1351.1991
更新日期:1991-04-01 00:00:00
abstract::The serotype-specific polysaccharide of Streptococcus mutans AHT (serotype a) was shown to be loosely associated with the cell surface of this organism. The antigen was extracted from whole cells by boiling in sodium acetate buffer, pH 4.0, for 10 min. The purified product was found to be a diheteroglycan of galactose...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1177-1185.1979
更新日期:1979-12-01 00:00:00
abstract::The fliA gene of Legionella pneumophila encoding the alternative sigma(28) factor was inactivated by introducing a kanamycin resistance cassette. Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin. Reporter gene assays indicated that the flaA pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1604-1608.2002
更新日期:2002-03-01 00:00:00
abstract::Broth culture supernatants from Tox+ Helicobacter pylori strains induce vacuolation of HeLa cells in vitro and contain VacA in concentrations that are higher than those found in supernatants from Tox- H. pylori strains. To investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cyto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3088-3094.1998
更新日期:1998-07-01 00:00:00
abstract::The Legionella pneumophila major secretory protein (Msp) is a Zn2+ metalloprotease whose function in pathogenesis is unknown. The structural gene for the Msp protease, mspA, was isolated from an L. pneumophila genomic library. In Escherichia coli which contain plasmids with the mspA gene, Msp protein and activity are ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2585-2592.1990
更新日期:1990-08-01 00:00:00
abstract::A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard err...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.12-19.1977
更新日期:1977-04-01 00:00:00
abstract::It has been previously reported that although inducible nitric oxide synthase (iNOS) gene knockout (NOS2(-/-)) mice resolve Chlamydia trachomatis genital infection, the production of reactive nitrogen species (RNS) via iNOS protects a significant proportion of mice from hydrosalpinx formation and infertility. We now r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7374-7379.2001
更新日期:2001-12-01 00:00:00
abstract::Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of bubonic and pneumonic plague. We identified a homolog of the Salmonella enterica serovar Typhimurium lipoprotein (lpp) gene in Yersinia species and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01529-07
更新日期:2008-04-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, is introduced into human hosts via tick bites. Among the cell types present in the skin which may initially contact spirochetes are mast cells. Since spirochetes are known to activate a variety of cell types in vitro, we tested whether B. burgdorferi spirochetes could...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1107-1115.1999
更新日期:1999-03-01 00:00:00
abstract::Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.516-522.1978
更新日期:1978-11-01 00:00:00
abstract::The pathogenicities of three proteases from Serratia marcescens, two proteases from Pseudomonas aeruginosa, and one thermolysin from Bacillus stearothermophilus were examined. All proteases tested caused acute liquefactive necrosis of the cornea and descemetocele formation in guinea pig eyes after intrastromal injecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2509-2517.1987
更新日期:1987-10-01 00:00:00
abstract::The adherence protein (P1 protein) of Mycoplasma pneumoniae was purified by electroelution and cleaved with cyanogen bromide. The resulting peptides were separated by two-dimensional electrophoresis. Spots reacting in Western immunoblots with two attachment-inhibiting monoclonal antibodies were isolated, and the amino...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.685-688.1989
更新日期:1989-03-01 00:00:00
abstract::A better knowledge on how immune responses are initiated in mucosal tissues would facilitate the design of new mucosal vaccines, as well as improve our understanding on host defense against infection. We investigated the mechanisms of adjuvanticity of the Mycoplasma-derived macrophage-activating 2-kDa lipopeptide (MAL...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.6978-6986.2004
更新日期:2004-12-01 00:00:00
abstract::Group A streptococcal pyrogenic exotoxin (SPE) type C, produced by strain T18P grown in the presence of 32P, was separated from culture supernatant fluids by using alcohol precipitation. The resulting toxin (EtOH-1) contained 3 X 10(6) to 5 X 10(6) cpm of 32P per milligram of protein. The radiolabel migrated with SPE ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.585-589.1979
更新日期:1979-11-01 00:00:00
abstract::The chlamydial inclusion occupies a unique niche within the eukaryotic cell that does not interact with endocytic compartments but instead is fusogenic with a subset of sphingomyelin-containing exocytic vesicles. The Chlamydia trachomatis inclusion acquires these distinctive properties by as early as 2 h postinfection...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.973-984.2003
更新日期:2003-02-01 00:00:00
abstract::The appearance of lymphotoxin in the culture fluid of phytohemagglutinin (PHA)-stimulated mouse spleen cells required two cell fractions that were separated by adherence to plastic. Upon stimulation with PHA, neither cell fraction alone produced significant amounts of lymphotoxin; however, when the cell fractions were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.1028-1035.1982
更新日期:1982-06-01 00:00:00
abstract::We previously reported that infection by Mycobacterium tuberculosis, the causative agent of tuberculosis, leads to secretion of alpha/beta interferon (IFN-alpha/beta). While IFN-alpha/beta ordinarily stimulates formation of signal transducer and stimulator of transcription-1 (STAT-1) homodimers and IFN-stimulated gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2487-2497.2003
更新日期:2003-05-01 00:00:00
abstract::The effects of pathologically relevant concentrations (0.38 to 12.5 microM) of the proinflammatory, Pseudomonas aeruginosa-derived pigment 1-hydroxyphenazine (1-hp) on Ca2+ metabolism and intracellular cyclic AMP (cAMP) in N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP; 1 microM)-activated human neutrophils, as w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5157-5162.1999
更新日期:1999-10-01 00:00:00
abstract::Neisseria meningitidis is a major cause of sepsis and meningitis but is also a common commensal, present in the nasopharynx of between 8 and 20% of healthy individuals. During carriage, the bacterium is found on the surface of the nasopharyngeal epithelium and in deeper tissues, while to develop disease the meningococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01377-09
更新日期:2010-09-01 00:00:00
abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1055-1060.1992
更新日期:1992-03-01 00:00:00
abstract::The neurological syndrome caused by Plasmodium berghei ANKA in rodents partially mimics the human disease. Several rodent models of cerebral malaria (CM) exist for the study of the mechanisms that cause the disease. However, since common laboratory mouse strains have limited gene pools, the role of their phenotypic va...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2049-2056.2002
更新日期:2002-04-01 00:00:00
abstract::Bordetella bronchiseptica is closely related to Bordetella pertussis, which produces respiratory disease primarily in mammals other than humans. However, its importance as a human pathogen is being increasingly recognized. Although a large amount of research on Bordetella has been generated regarding protein virulence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.1791-1798.2002
更新日期:2002-04-01 00:00:00
abstract::Antiserum to a synthetic peptide, CTP3, consisting of residues 50 to 64 of the B subunit of cholera enterotoxin (CT), a sequence which is conserved in heat-labile enterotoxins from Escherichia coli strains of human (H-LT) or porcine (P-LT) origin, cross-reacted with and neutralized all three enterotoxins. It also cros...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.2.562-567.1986
更新日期:1986-05-01 00:00:00
abstract::This study determined the role of crl in the production of curli by, the hemagglutination activity of, and fibronectin binding by avian pathogenic Escherichia coli chi 7122. Curli, an extracellular structure that binds fibronectin, was recently described (A. Olsén, A. Jonsson, and S. Normark, Nature [London] 338:652-6...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4460-4467.1992
更新日期:1992-11-01 00:00:00
abstract::Carbon storage regulator A (CsrA) is an RNA binding protein that has been characterized in many bacterial species to play a central regulatory role by modulating several metabolic processes. We recently showed that a homolog of CsrA in Borrelia burgdorferi (CsrA(Bb), BB0184) was upregulated in response to propagation ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00882-10
更新日期:2011-02-01 00:00:00