Abstract:
:The Legionella pneumophila major secretory protein (Msp) is a Zn2+ metalloprotease whose function in pathogenesis is unknown. The structural gene for the Msp protease, mspA, was isolated from an L. pneumophila genomic library. In Escherichia coli which contain plasmids with the mspA gene, Msp protein and activity are found in the periplasmic space and the cytoplasm. Transposon mutagenesis with Tn9 of an mspA-containing plasmid in E. coli yielded mutants which no longer expressed protease activity and others with increased protease activity. These results suggested that mspA expression might be regulated. Msp was shown to be produced at a much higher level in L. pneumophila grown in rich compared to semidefined media. A Tn9 insertion which abolishes Msp expression was introduced into the L. pneumophila genome. This mspA::Tn9 L. pneumophila strain showed no detectable production of Msp by immunoblot analysis, and it had less than 0.1% of the protease activity found in the wild-type strain. This mutant was fully capable of growing within and killing human macrophages derived from the HL-60 cell line.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Szeto L,Shuman HAdoi
10.1128/IAI.58.8.2585-2592.1990subject
Has Abstractpub_date
1990-08-01 00:00:00pages
2585-92issue
8eissn
0019-9567issn
1098-5522journal_volume
58pub_type
杂志文章abstract::While several diseases associated with Chlamydia psittaci infection have been reported in Phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. In this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1897-1900.1988
更新日期:1988-08-01 00:00:00
abstract::Murine bone marrow-derived dendritic cells (DC) can phagocytose and process Salmonella enterica serovar Typhimurium for peptide presentation on major histocompatibility complex class I (MHC-I) and MHC-II molecules. To investigate if a serovar Typhimurium encounter with DC induces maturation and downregulates their abi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.11.6311-6320.2000
更新日期:2000-11-01 00:00:00
abstract::The cytopathic effect (CPE) of Escherichia coli producing cytotoxic necrotizing factor type 1 (CNF1) was investigated by using a human epithelial cell (HeLa) model of infection with CNF1-producing E. coli BM2-1. This strain was shown to bind loosely, but massively, to HeLa cells. A 4-h interaction between bacteria and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1694-1705.1996
更新日期:1996-05-01 00:00:00
abstract::In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxing) from the intrac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7657-7668.2005
更新日期:2005-11-01 00:00:00
abstract::Light and electron microscopy with histochemical staining were used to estimate exopolysaccharide production by strains of viridans streptococci recovered from patients with endocarditis. Six strains were selected for study because they represented a wide range of in vitro polysaccharide production. By light microscop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.359-367.1984
更新日期:1984-01-01 00:00:00
abstract::The ability of hemagglutinating and poorly hemagglutinating strains of the gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled laminin was quantitated in a liquid phase assay. Although all strains bound laminin, some hemagglutinating strains were good binders of laminin (maximum of 31% binding), whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3640-3648.1994
更新日期:1994-09-01 00:00:00
abstract::Clostridium perfringens enterotoxin is the major virulence factor involved in the pathogenesis of C. perfringens type A food poisoning and several non-food-borne human gastrointestinal illnesses. The enterotoxin gene, cpe, is located on the chromosome of food-poisoning isolates but is found on a large plasmid in non-f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3483-3487.2001
更新日期:2001-05-01 00:00:00
abstract::A strain of a nutritionally deficient streptococcus was shown to undergo morphological aberrations according to pyridoxal concentrations in the growth medium. Filamentous rod-shaped cells, observed by electron microscopy, predominated in the presence of decreasing concentrations. Multiple invaginations in the outer ce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.414-417.1983
更新日期:1983-10-01 00:00:00
abstract::Hemolytic-uremic syndrome (HUS) is a serious complication predominantly associated with infection by enterohemorrhagic Escherichia coli (EHEC), such as E. coli O157:H7. EHEC can produce Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2), both of which are exotoxins comprised of active (A) and binding (B) subunits. In pi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.612-619.2002
更新日期:2002-02-01 00:00:00
abstract::Malaria in pregnancy is associated with placental accumulation of Plasmodium falciparum-infected erythrocytes (IE) that adhere to chondroitin sulfate A (CSA). Adhesion is mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1), a variant parasite protein expressed on the surface of IE and encoded by var gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2848-2856.2005
更新日期:2005-05-01 00:00:00
abstract::A key function of monocytes/macrophages (Mphi) is to present antigens to T cells. However, upon interaction with bacteria, Mphi lose their ability to effectively present soluble antigens. This functional loss was associated with alterations in the expression of adhesion molecules and CD14 and a reduction in the uptake...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1338-1346.1999
更新日期:1999-03-01 00:00:00
abstract::We report on the colonization of murine trigeminal ganglia after sequential infection of mice by herpes simplex viruses (HSVs). In preliminary studies, we have established that whereas the HSV-1(F) strain efficiently colonizes ganglia when inoculated by either the ear or eye routes, the HSV-1 X HSV-2 recombinant C7D c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.2.702-708.1983
更新日期:1983-08-01 00:00:00
abstract::Vibrio cholerae is the causal organism of the diarrheal disease cholera. The rugose variant of V. cholerae is associated with the secretion of an exopolysaccharide. The rugose polysaccharide has been shown to confer increased resistance to a variety of agents, such as chlorine, bioacids, and oxidative and osmotic stre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1967-1974.2000
更新日期:2000-04-01 00:00:00
abstract::Transplacental infection of mouse fetuses with Venezuelan equine encephalomyelitis was produced by intraperitoneal injection of dams at various stages of gestation with 10(3) suckling mouse intracerebral median lethal doses of an attenuated strain (TC-83). Virus inoculation, at times ranging from 6 days prior to matin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.339-343.1972
更新日期:1972-09-01 00:00:00
abstract::We have previously shown that a Pasteurella multocida cexA mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1943-1946.2001
更新日期:2001-03-01 00:00:00
abstract::A gene bank of group A Streptococcus strain Manfredo (M protein serotype 5) was constructed with a bacteriophage lambda vector-Escherichia coli K-12 host system and screened by immunoblotting hybrid phage plaques with antisera raised to purified pep M5 (serotype 5 M protein fragment released from the streptococcal cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.190-197.1985
更新日期:1985-04-01 00:00:00
abstract::We have constructed an improved recombination-based in vivo expression technology (RIVET) and used it as a screening method to identify Vibrio cholerae genes that are transcriptionally induced during infection of infant mice. The improvements include the introduction of modified substrate cassettes for resolvase that ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.972-980.2005
更新日期:2005-02-01 00:00:00
abstract::Vero cytotoxin-producing Escherichia coli (VTEC) strains have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. Since adherence of enteric pathogens to epithelial surfaces is often a prerequisite for the subsequent delivery of bacterial enterotox...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
abstract::To determine optimal strategies to induce specific-antibody-secreting cells (specific ASC) in the rectal and vaginal mucosae, we immunized monkeys with a prototype mucosal immunogen, cholera toxin (CT), given locally or via gastric or parenteral administration. Repeated rectal or vaginal CT immunizations induced stron...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5889-5896.1998
更新日期:1998-12-01 00:00:00
abstract::Thirty percent of Streptococcus pneumoniae isolates contain pilus islet 1, coding for a pilus composed of the backbone subunit RrgB and two ancillary proteins, RrgA and RrgC. RrgA is the major determinant of in vitro adhesion associated with pilus 1, is protective in vivo in mouse models, and exists in two variants (c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00601-10
更新日期:2010-12-01 00:00:00
abstract::Glucan-binding protein B (GbpB) from Streptococcus mutans has been shown to induce protective immunity to dental caries in experimental models. Having recently sequenced the gbpB gene, our objective in this study was to identify immunogenic regions within the GbpB sequence for use in subunit vaccines. Potential region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1179-1184.2003
更新日期:2003-03-01 00:00:00
abstract::Listeria monocytogenes is an intracellular bacterial pathogen which causes bacteremia and has a tropism for the central nervous system and a propensity to cause maternofetal infection. L. monocytogenes has been shown to be an effective prophylactic and a therapeutic vaccine vector for viral and tumor antigens in anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3592-3601.2002
更新日期:2002-07-01 00:00:00
abstract::Merozoite surface protein 2 (MSP2) is an abundant glycosylphosphatidylinositol (GPI)-anchored protein of Plasmodium falciparum, which is a potential component of a malaria vaccine. As all forms of MSP2 can be categorized into two allelic families, a vaccine containing two representative forms of MSP2 may overcome the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00665-12
更新日期:2012-12-01 00:00:00
abstract::Escherichia coli 536 (O6:K15:H31), which was isolated from a case of urinary tract infection, determines high nephropathogenicity in a rat pyelonephritis system as measured by renal bacterial counts 7 days after infection. The loss of S fimbrial adhesin formation (Sfa-) (mannose-resistant hemagglutination [Mrh-] and f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.761-767.1986
更新日期:1986-12-01 00:00:00
abstract::Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5995-6004.2005
更新日期:2005-09-01 00:00:00
abstract::A progressive increase in infections with multiresistant Enterococcus faecium has been reported, especially in cancer patients and neutropenic patients. Despite its increasing importance as a nosocomial pathogen, knowledge of the pathogenesis of E. faecium infections is highly limited. In this study, we investigated t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00863-08
更新日期:2009-01-01 00:00:00
abstract::Erythrocytic malaria parasites degrade hemoglobin as a principal source of amino acids for parasite protein synthesis. We have previously shown that a Plasmodium falciparum trophozoite cysteine proteinase, now termed falcipain, is required for hemoglobin degradation, and we have hypothesized that this proteinase is re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2120-2125.1995
更新日期:1995-06-01 00:00:00
abstract::When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. bur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2104-2107.1993
更新日期:1993-05-01 00:00:00
abstract::The outcome of coccidioidomycosis depends to a large extent on the effectiveness of the T-cell-mediated immune (CMI) response to the fungal pathogen. For this reason, identification of Coccidioides immitis antigens which stimulate T cells is important for understanding the nature of host defense against the organism a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.424-431.1998
更新日期:1998-02-01 00:00:00
abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00