Abstract:
:Erythrocytic malaria parasites degrade hemoglobin as a principal source of amino acids for parasite protein synthesis. We have previously shown that a Plasmodium falciparum trophozoite cysteine proteinase, now termed falcipain, is required for hemoglobin degradation, and we have hypothesized that this proteinase is responsible for initial cleavages of hemoglobin. To further evaluate the biological role of falcipain, we expressed the enzyme in bacterial and viral expression systems. After expression in the baculovirus system, falcipain was enzymatically active and had biochemical properties very similar to those of the native proteinase. Recombinant falcipain rapidly hydrolyzed both denatured and native hemoglobin. Hemoglobin hydrolysis was blocked by cysteine proteinase inhibitors but not by inhibitors of other classes of proteinases. Our results support our hypothesis that falcipain is a critical malarial hemoglobinase that is responsible for both initial cleavages of hemoglobin and the subsequent hydrolysis of globin into small peptides.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Salas F,Fichmann J,Lee GK,Scott MD,Rosenthal PJdoi
10.1128/IAI.63.6.2120-2125.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
2120-5issue
6eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::Studies with animal models have demonstrated that viral respiratory tract infections suppress bacterial clearance processes in the lung and that this alteration in host defenses appears to explain the excessive mortality from bacterial pneumonia during influenza epidemics. However, since the pathogenesis of postinflue...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1102-1108.1983
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pub_type: 杂志文章
doi:10.1128/IAI.53.3.522-529.1986
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1581-1586.2001
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6665-6671.2002
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journal_title:Infection and immunity
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doi:10.1128/IAI.00287-10
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3767-3774.1998
更新日期:1998-08-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.01199-12
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.4351-4355.1996
更新日期:1996-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.581-586.1984
更新日期:1984-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/iai.68.5.2525-2534.2000
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.2.287-292.1974
更新日期:1974-08-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.3.1364-1373.2004
更新日期:2004-03-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1997-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.664-669.1985
更新日期:1985-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.2.555-560.1985
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.4.1189-1195.1997
更新日期:1997-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.420-423.1971
更新日期:1971-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.493-501.1984
更新日期:1984-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00873-08
更新日期:2009-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4788-4794.1996
更新日期:1996-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3724-3729.1993
更新日期:1993-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.254-262.1977
更新日期:1977-01-01 00:00:00