Abstract:
:Previous studies have indicated that the ability to bind to fibronectin is a key feature in successful cell invasion by Campylobacter jejuni. Given the spatial distribution of fibronectin and the architecture of the epithelium, this suggests the possibility that C. jejuni cell invasion might preferentially occur at the basolateral cell surface. To test this hypothesis, we examined the interaction of C. jejuni with T84 human colonic cells. When grown under the appropriate conditions, T84 cells form a polarized cell monolayer. C. jejuni translocation of a T84 cell monolayer appeared to occur via a paracellular (extracellular) route as opposed to a transcellular (intracellular) route based on the finding that a C. jejuni noninvasive mutant translocated as efficiently as its isogenic parent. Additional studies revealed that two distinct C. jejuni wild-type isolates could compete with one another for host cell receptors, whereas a C. jejuni fibronectin-binding-deficient mutant could not compete with a wild-type isolate for host cell receptors. Further, C. jejuni adherence and internalization were significantly inhibited by antifibronectin antibodies but only when cells were first treated with EGTA to expose basolateral cell surfaces. Together, these results support the theory that C. jejuni invasion occurs preferentially at the basolateral surface of eukaryotic cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Monteville MR,Konkel MEdoi
10.1128/iai.70.12.6665-6671.2002keywords:
subject
Has Abstractpub_date
2002-12-01 00:00:00pages
6665-71issue
12eissn
0019-9567issn
1098-5522journal_volume
70pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1987-07-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.66.7.3088-3094.1998
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doi:10.1128/iai.68.6.3251-3260.2000
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.88-98.1978
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1260-1265.1974
更新日期:1974-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.739-744.1982
更新日期:1982-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1989-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.592-600.1983
更新日期:1983-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.918-926.1981
更新日期:1981-09-01 00:00:00