Abstract:
:Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the locus for enterocyte effacement (LEE) pathogenicity island, but a proportion of isolates from patients with severe disease do not carry LEE and very little is known about virulence factors in these organisms. LEE-negative strains of EHEC typically express Shiga toxin 2 and carry a large plasmid that encodes the production of EHEC hemolysin. In this study, we determined the nucleotide sequence of the transfer region of pO113, the large hemolysin plasmid from LEE-negative EHEC O113:H21 (EH41). This 63.9-kb region showed a high degree of similarity with the transfer region of R64, and pO113 was capable of self-transmission at low frequencies. Unlike R64 and the related dot/icm system of Legionella pneumophila, however, pO113 was unable to mobilize RSF1010. In addition, the pO113 transfer region encoded a novel high-molecular-weight serine protease autotransporter of Enterobacteriaceae (SPATE) protein, termed EpeA. Like other SPATEs, EpeA exhibited protease activity and mucinase activity, but expression was not associated with a cytopathic effect on epithelial cells. Analysis of a second high-molecular-weight secreted protein revealed that pO113 also encodes EspP, a cytopathic SPATE identified previously in EHEC O157:H7. The nucleotide sequences encoding the predicted beta-domains of espP and epeA were identical and also shared significant homology with a third SPATE protein, EspI. Both espP and epeA were detected in several LEE-negative clinical isolates of EHEC and thus may contribute to the pathogenesis of this subset of EHEC.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Leyton DL,Sloan J,Hill RE,Doughty S,Hartland ELdoi
10.1128/iai.71.11.6307-6319.2003keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
6307-19issue
11eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::Antiserum and purified immunoglobulin directed against Mycoplasma pneumoniae membrane protein P1 were examined for their influence on mycoplasma viability, metabolism, and cytadsorption. Anti-P1 immunoglobulin inhibited adherence of M. pneumoniae to hamster tracheal rings in vitro by up to 80% and inhibited hemadsorpt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1180-1186.1983
更新日期:1983-03-01 00:00:00
abstract::Alpha-toxin, the major cytolysin of Staphylococcus aureus, preferentially attacks human platelets and cultured monocytes, thereby promoting coagulation and the release of interleukin-1 and tumor necrosis factor. Titers of naturally occurring antibodies in human blood are not high enough to substantially inhibit these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3214-3220.1989
更新日期:1989-10-01 00:00:00
abstract::Scrub typhus is a potentially lethal infection that is caused by the obligate intracellular bacterium Orientia tsutsugamushi The roles of Toll-like receptor 2 (TLR2) and TLR4 in innate recognition of O. tsutsugamushi have not been elucidated. By overexpression of TLR2 or TLR4 in HEK293 cells, we demonstrated that TLR2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00185-16
更新日期:2016-11-18 00:00:00
abstract::Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4883-4889.1995
更新日期:1995-12-01 00:00:00
abstract::An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5238-5244.2003
更新日期:2003-09-01 00:00:00
abstract::Gram-negative bacterial cells exposed to a complement source may carry membrane attack complexes containing variable numbers of C9 molecules per C5b-8 site. In order to investigate the assembly of this complex, the ability of C9 molecules to bind to C5b-8 complexes was compared with the binding characteristics of C9 f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2800-2805.1994
更新日期:1994-07-01 00:00:00
abstract::To assess the relationship between the within-host diversity of malaria infections and the susceptibility of the host to subsequent infection, we genotyped 60 children's successive infections from birth through 3 years of life. MSP-1 Block2 genotypes were used to estimate the complexity of infection (COI). Malaria tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.12.7783-7792.2001
更新日期:2001-12-01 00:00:00
abstract::Chlamydial inclusions were demonstrated by indirect immunofluorescence (IF) with antiserum to the chlamydial group antigen when McCoy cell monolayers infected with either Chlamydia trachomatis or Chlamydia psittaci were fixed in formaldehyde or paraformaldehyde, provided the monolayer was not allowed to dry. If these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.561-570.1981
更新日期:1981-11-01 00:00:00
abstract::3-O-methylmannose isolated from Coccidioides immitis was characterized by gas chromatographic comparison of derivatives with those of synthetic 3-O-methylmannose. Gas chromatographic behavior of three derivatives of the natural and synthetic sugars was identical. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.660-661.1971
更新日期:1971-11-01 00:00:00
abstract::A dextranase preparation (AD17) partially purified from a culture liquor of Spicaria violacea strain IFO 6120 significantly inhibited the formation of artifcial dental plaque on a steel wire or on an extracted tooth surface. Changes in the surface morphology of Streptococcus mutans cells due to AD17 action were studie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.6.1415-1425.1975
更新日期:1975-12-01 00:00:00
abstract::Understanding the extent to which genetic factors influence the immune response is important in the development of subunit vaccines. Associations with HLA gene polymorphisms appear insufficient to explain the range of variation in immune responses to vaccines and to infections by major pathogens. In this study of Gamb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.872-876.1997
更新日期:1997-03-01 00:00:00
abstract::Strains Inaba 569B and Ogawa B1307 of Vibrio cholerae were grown in shaken Casamino Acids-yeast extract-glucose medium at 30 and 37 C for 4 days. Time courses of production of neuraminidase (NM), mucinase (MC), proteinase (PR), lytic factor (LF), and vascular permeability factor (PF) were compared by examining culture...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-01-01 00:00:00
abstract::Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1391-1396.1974
更新日期:1974-12-01 00:00:00
abstract::The antigens of Haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a protein A-peroxidase conjugate. The same two 76K and 40K antigens were predominant in whole-bacterium preparations and in outer-membrane-enric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1381-1386.1987
更新日期:1987-06-01 00:00:00
abstract::Binding of Escherichia coli strain 431 heat-stable enterotoxin (STa) and activation of intestinal particulate guanylate cyclase by E. coli STa were studied with rat intestinal epithelial cells and brush border membranes (BBMs). The rates of guanylate cyclase stimulation by 431 STa in cells and BBMs were rapid, with ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.537-543.1984
更新日期:1984-11-01 00:00:00
abstract::Streptococcus pneumoniae is a leading cause of bacterial pneumonia, meningitis, and sepsis in children. Human immunity to pneumococcal infections has been assumed to depend on anticapsular antibodies. However, recent findings from murine models suggest that alternative mechanisms, dependent on T helper cells, are also...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05286-11
更新日期:2011-10-01 00:00:00
abstract::Pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. P. multocida strains simultaneously express two lipopolysaccharide (LPS) glycoforms (glycoforms A and B) that differ only in their inner core structure. Glycoform A contains a single 3-deoxy-d-manno-octulosonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00457-10
更新日期:2010-09-01 00:00:00
abstract::In this study we demonstrate the capability of Chlamydia trachomatis to infect cultured human mesothelial cell (MC) monolayers and to induce the production of the proinflammatory cytokines interleukin 1beta (IL-1beta) and IL-8. Seventy-two hours after initial infection, both the procoagulant activity of MC and the act...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2352-2355.1998
更新日期:1998-05-01 00:00:00
abstract::We have constructed an improved recombination-based in vivo expression technology (RIVET) and used it as a screening method to identify Vibrio cholerae genes that are transcriptionally induced during infection of infant mice. The improvements include the introduction of modified substrate cassettes for resolvase that ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.972-980.2005
更新日期:2005-02-01 00:00:00
abstract::The role of the spleen in hepatic amoebiasis in hamsters was studied. In hamsters receiving an intrahepatic inoculation of 10(5) trophozoites of axenic Entamoeba histolytica at 7 or 14 days postsplenectomy, the mean weight of metastatic foci increased significantly when compared with sham-splenectomized or intact cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.571-573.1981
更新日期:1981-02-01 00:00:00
abstract::Borrelias that cause Lyme disease lose the ability to infect and cause disease in laboratory animals following 10 to 16 passages of in vitro culture. In this study, clonal populations of the Sh-2-82 (Sh2) and B31 strains of Borrelia burgdorferi were isolated by subsurface plating on BSK-II agar plates and examined for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2206-2212.1995
更新日期:1995-06-01 00:00:00
abstract::Salivary gland lysates of the sand fly Lutzomyia longipalpis markedly enhance the course of infection with Leishmania major in mice. Here we examine various parameters of this phenomenon. The exacerbative effect of L. longipalpis salivary gland lysates occurred in five different mouse strains; however, the character o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1592-1598.1991
更新日期:1991-05-01 00:00:00
abstract::By using the powerful separation technique of capillary gas chromatography combined with the selectivity of mass spectrometric detection, muramic acid was not detectable in purified elementary bodies of Chlamydia psittaci Cal 10 (less than or equal to 0.006%) or C. trachomatis serovar E (less than or equal to 0.02%). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.835-837.1990
更新日期:1990-03-01 00:00:00
abstract::Two distinct monoclonal antibodies, one to pertussis toxin subunit S2, called 9G8, and another to subunits S2 and S3, called 11E6, were generated from the hybridomas of myeloma SP2/0 and spleen cells of BALB/c mice immunized mainly with the subunit S234 complex. Binding ability of 9G8 and 11E6 to the subunits was conf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.4.909-915.1987
更新日期:1987-04-01 00:00:00
abstract::Most vaccines are still given parenterally. Mucosal vaccination would offer different advantages over parenteral immunization, including blocking of the pathogens at the portal of entry. In this paper, nontoxic Escherichia coli heat-labile enterotoxin (LT) mutants and Supramolecular Biovector systems (SMBV) were evalu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.4785-4790.2002
更新日期:2002-09-01 00:00:00
abstract::Actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). The phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal DNA. A genomic fragment encoding the cytolysin and its flanking sequences was cloned ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4110-4116.1991
更新日期:1991-11-01 00:00:00
abstract::A toxin associated with Toxoplasma gondii infection was obtained from the trophozoites and culture medium used to propagate the parasite in cell cultures. The toxin, named Toxofactor (TF), administered parenterally or nonparenterally in adult mice, produces transient symptoms of lethargy, ruffled fur, and body weight ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1126-1135.1983
更新日期:1983-12-01 00:00:00
abstract::In this study, cytokine patterns produced by CD4(+) T cells isolated from antrum or corpus gastral biopsy specimens of 10 patients with Helicobacter pylori-positive gastritis were compared. To this end, expression of intracellular cytokines (interleukin-4 [IL-4] and gamma interferon) and of CD4 was assessed by flow cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5543-5546.1998
更新日期:1998-11-01 00:00:00
abstract::In vivo expression of pili by Escherichia coli in the urine of 41 adults with lower urinary tract infections was analyzed by immunostaining with polyclonal antiserum to type 1 and P pili. Type 1 pili were detected in 31 of 41 urine specimens, while P pili were detected in 6 of 18 specimens. The piliation status of bac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1656-1662.1989
更新日期:1989-06-01 00:00:00
abstract::Although the suckling mouse assay is widely used for the detection of heat-stable Escherichia coli enterotoxin (ST), few data have been published concerning the reproducibility, optimal growth, and test conditions of this assay. Four strains of toxigenic E. coli known to elaborate both heat-labile enterotoxin and ST o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.95-99.1976
更新日期:1976-07-01 00:00:00