Abstract:
:In vivo expression of pili by Escherichia coli in the urine of 41 adults with lower urinary tract infections was analyzed by immunostaining with polyclonal antiserum to type 1 and P pili. Type 1 pili were detected in 31 of 41 urine specimens, while P pili were detected in 6 of 18 specimens. The piliation status of bacterial populations in urine was heterogeneous, varying from predominantly piliated to a mixture of piliated and nonpiliated cells. Bacteria frequently adhered to exfoliated uroepithelial cells and leukocytes in urine. Expression of pili in vivo did not always correlate with the hemagglutination phenotype after growth in vitro. Strains isolated from different sites in the urogenital tract of two individuals showed phenotypic variation in the state of piliation. The results demonstrate that E. coli type 1 and P pili are expressed and are subject to variation in vivo during acute urinary tract infections in adults.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kisielius PV,Schwan WR,Amundsen SK,Duncan JL,Schaeffer AJdoi
10.1128/IAI.57.6.1656-1662.1989subject
Has Abstractpub_date
1989-06-01 00:00:00pages
1656-62issue
6eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::Parasite antigens which bind to host cell molecules of approximately 32 and 34 kilodaltons (kDa) were identified in supernatant fluids obtained from axenic cultures of Trypanosoma cruzi. These parasite components were first detected in culture supernatants obtained after 2 weeks in culture. Immunoblot analysis of cult...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1812-1816.1990
更新日期:1990-06-01 00:00:00
abstract::Development of hemolytic uremic syndrome (HUS) after infection by Shigella dysenteriae 1 or enterohemorrhagic Escherichia coli has been associated with the production of Shiga toxins (verotoxins). The putative target of Shiga toxins in HUS is the renal microvascular endothelium. This report shows that preincubation of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3886-3891.1993
更新日期:1993-09-01 00:00:00
abstract::An antigen recognized by monoclonal antibody specific for Bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. The purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.366-371.1986
更新日期:1986-08-01 00:00:00
abstract::To determine optimal strategies to induce specific-antibody-secreting cells (specific ASC) in the rectal and vaginal mucosae, we immunized monkeys with a prototype mucosal immunogen, cholera toxin (CT), given locally or via gastric or parenteral administration. Repeated rectal or vaginal CT immunizations induced stron...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5889-5896.1998
更新日期:1998-12-01 00:00:00
abstract::Colostrum was collected and pooled from each of five cows in three experimental groups: group I cows received intramuscular and intramammary inoculations of adjuvanted modified live Ohio Agricultural Research and Development Center rotavirus vaccine; group II cows were injected intramuscularly with a commercial modifi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1118-1131.1983
更新日期:1983-09-01 00:00:00
abstract::Following intranasal inoculation, Vibrio cholerae KFV101 (DeltactxAB DeltahapA DeltahlyA DeltartxA) colonizes and stimulates tumor necrosis factor alpha and interleukin 1beta (IL-1beta) in mice, similar to what occurs with isogenic strain P4 (DeltactxAB), but is less virulent and stimulates reduced levels of IL-6, dem...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.6157-6164.2005
更新日期:2005-09-01 00:00:00
abstract::The ability of some typical enteropathogenic Escherichia coli (EPEC) strains to adhere to, invade, and increase interleukin-8 (IL-8) production in intestinal epithelial cells in vitro has been demonstrated. However, few studies regarding these aspects have been performed with atypical EPEC (aEPEC) strains, which are e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00177-09
更新日期:2009-10-01 00:00:00
abstract::We report here invasin-dependent and invasin-independent mechanisms in which the enteropathogen Yersinia enterocolitica is able to disseminate from the lumen of the small intestine to the spleen. The invasin-dependent route is clearly discernible in mice devoid of intestinal Peyer's patches and mesenteric lymph nodes....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8453-8455.2005
更新日期:2005-12-01 00:00:00
abstract::The viaB locus coding for the Vi antigen of Salmonella typhi Ty2 was cloned on a 40.6-kilobase fragment into the cosmid vector pHC79. The live, oral, attenuated Vi-negative S. typhi Ty21a vaccine strain was transformed with the recombinant cosmid encoding the viaB locus. Homologous recombination of the viaB locus into...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3863-3868.1989
更新日期:1989-12-01 00:00:00
abstract::The surface coat of Trypanosoma brucei, formed by about 10(7) molecules of the membrane-form variant surface glycoprotein (mfVSG) per cell, is generally considered to constitute a barrier against the access of antibodies directed to invariant surface proteins. The recent characterization of two invariant surface glyco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4540-4545.1993
更新日期:1993-11-01 00:00:00
abstract::This study was carried out to determine the role of nitric oxide (NO) in Staphylococcus aureus infection in mice. NO production in spleen cell cultures was induced by heat-killed S. aureus. Expression of mRNA of the inducible isoform of NO synthase (iNOS) was induced in the spleens and kidneys of S. aureus-infected mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.3.1017-1022.1998
更新日期:1998-03-01 00:00:00
abstract::A procedure was developed to purify dermonecrotic toxin (DNT) from a sonic extract of a serotype D strain of Pasteurella multocida. Sonic extract containing DNT was applied to a DEAE-Sephacel column and eluted by a linear gradient of NaCl. Upon rechromatographing, fractions with dermonecrotic activity for guinea pigs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.429-434.1984
更新日期:1984-11-01 00:00:00
abstract::Cytomegalovirus (CMV) infections are common in renal transplant recipients. We studied 23 recipients prospectively to determine whether infections by other herpes-group and non-herpes-group viruses were also present. Sera, obtained at the time of surgery and periodically thereafter, were tested for antibody to CMV, he...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.970-975.1976
更新日期:1976-10-01 00:00:00
abstract::Recombinant major surface antigen (P30), which was produced as a glutathione S-transferase (EC 2.5.1.18) fusion protein of Toxoplasma gondii, was found to be able to activate macrophages to kill T. gondii in vitro. The macrophage activation was due to P30 in the fusion protein, not to glutathione S-transferase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2851-2852.1991
更新日期:1991-08-01 00:00:00
abstract::Trypanosome-activated mouse peritoneal macrophages phagocytized and digested Trypanosoma brucei in vitro and in vivo, but in the absence of specific antiserum and complement the degree of phagocytosis was minimal. Ultrastructurally, the parasites attached to the macrophage by their flagella, and ingestion proceeded fl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.972-982.1978
更新日期:1978-03-01 00:00:00
abstract::Cytokine gene expression in cells migrating in afferent and efferent intestinal lymph was monitored for extended time periods in individual sheep experimentally infected with the nematode Trichostrongylus colubriformis. Animals from stable selection lines with increased levels of either genetic resistance (R) or susce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2175-2183.2005
更新日期:2005-04-01 00:00:00
abstract::In this communication, we examine the specificity of anti-herpes simplex virus (HSV) cytotoxic T lymphocytes (CTL). Serological studies of the two related HSV serotypes (HSV-1 and HSV-2) have revealed both type-specific and cross-reactive antigenic determinants in the viral envelope and on the surface of infected cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.795-803.1981
更新日期:1981-12-01 00:00:00
abstract::Serum was necessary for optimal phagocytosis of Aspergillus fumigatus spores by human leukocytes, and its opsonic capacity was greatly diminished by heat inactivation (56 C, 30 min). A germination assay, described in this report, was developed to study the fate of phagocytized spores. After incubation for 3 hr with no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.1.4.345-350.1970
更新日期:1970-04-01 00:00:00
abstract::Purified and concentrated Venezuelan equine encephalitis (VEE) virus derived from tissue cultures, rendered noninfectious by ionizing radiation with retention of in vitro serological activity, also retained a high level of immunogenicity. In mice, fluid vaccines afforded excellent protection against lethal challenge w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.4.574-579.1971
更新日期:1971-04-01 00:00:00
abstract::Porphyromonas gingivalis, a periodontopathic bacterium, is known to invade oral epithelial cells in periodontal lesions, although the mechanism is unclear. In the present study, goat polyclonal anti-intercellular adhesion molecule 1 (anti-ICAM-1) antibody inhibited the invasion of P. gingivalis into KB cells (human or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6290-6298.2005
更新日期:2005-10-01 00:00:00
abstract::Yersinia pseudotuberculosis inv mutant strains cured of the virulence plasmid exhibit thermoinducible adhesion to cultured mammalian cells. To identify the genes responsible for this phenotype, Y. pseudotuberculosis homologs of the Y. enterocolitica ail and the Y. pestis psa loci were identified. Mutations in the Y. p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2483-2489.1996
更新日期:1996-07-01 00:00:00
abstract::Localized juvenile periodontitis (LJP) runs in families, and a predisposition to develop disease appears to be inherited in an autosomal dominant fashion. Patients with LJP have elevated levels of serum immunoglobulin G2 (IgG2), and this is most striking in black LJP patients. We hypothesized that the markedly elevate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2004-2009.1996
更新日期:1996-06-01 00:00:00
abstract::The in vitro effects of Pseudomonas aeruginosa elastase (P. aeruginosa E) and of human leukocyte elastase on human airway lysozyme (HAL) were investigated. P. aeruginosa E inactivated and cleaved HAL, whereas human leukocyte elastase had no effect. Total inactivation of HAL by P. aeruginosa E was observed after 120 mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.2.555-560.1985
更新日期:1985-02-01 00:00:00
abstract::The acute phase of Chagas' disease is accompanied by immunosuppression. To explore the underlying mechanism(s), we used an in vitro culture system in which the capacities of activated human peripheral blood mononuclear cells to express interleukin-2 receptors (IL-2R) and proliferate are markedly inhibited in the prese...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.8.2301-2305.1989
更新日期:1989-08-01 00:00:00
abstract::A water-soluble acylpolygalactosyl (APG) of 34 kDa was obtained from the Klebsiella pneumoniae membrane by alkaline hydrolysis and delipidation. APG comprises a poly(1,3)galactose chain, a core, and a lipid moiety made of a glucosamine disaccharide with two N-linked beta OH-myristates. The monocyte binding sites for A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1520-1527.1994
更新日期:1994-05-01 00:00:00
abstract::Infection with mycobacterial species, including Mycobacterium tuberculosis, has long been implicated in the etiopathology of rheumatoid arthritis (RA) on the basis of clinical and pathological similarities between tuberculosis and RA. Despite evidence of immune responses to mycobacterial antigens in RA patient synovia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1821-1831.2001
更新日期:2001-03-01 00:00:00
abstract::Staphylococcal enterotoxins A through D (SEA through SED) and toxic shock syndrome toxin-1 display superantigen properties, i.e., they stimulate a great fraction of T cells expressing certain T-cell receptor V beta sequences. Using a newly established rat model of septic Staphylococcus aureus arthritis, we have recent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.4185-4187.1995
更新日期:1995-10-01 00:00:00
abstract::A murine model system was established to study immune responses to the Pnu-Imune vaccine, which is made up of 23 different pneumococcal capsular polysaccharides. In this animal model, antibody-forming cell responses to 21 of 23 individual polysaccharides in the vaccine were detected. The Pnu-Imune vaccine elicited goo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2329-2336.1992
更新日期:1992-06-01 00:00:00
abstract::Gram-negative organisms incorporate hydroxy fatty acids into the lipid A moiety of lipopolysaccharide (LPS), and in the case of some members of the family Enterobacteriaceae, hydroxy fatty acids are incorporated exclusively into lipid A. However, a limited number of Bacteroides species have been shown to incorporate s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3753-3760.1994
更新日期:1994-09-01 00:00:00
abstract::Ocular exposure of mice to live elementary bodies of Chlamydia trachomatis serovar A results in immunological sensitization of the mice. This reactivity is manifested by the development of early (5 h) and delayed-type (24 h) dermal reactivity and serovar-specific antibody formation against either live or irradiated (1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.9-12.1986
更新日期:1986-10-01 00:00:00