Abstract:
:The viaB locus coding for the Vi antigen of Salmonella typhi Ty2 was cloned on a 40.6-kilobase fragment into the cosmid vector pHC79. The live, oral, attenuated Vi-negative S. typhi Ty21a vaccine strain was transformed with the recombinant cosmid encoding the viaB locus. Homologous recombination of the viaB locus into the chromosome of S. typhi Ty21a was induced by UV irradiation, and Vi-positive recombinants were selected in the presence of D-cycloserine. One such isolate, termed WR4103, contained no plasmids or the attendant antibiotic resistance markers and expressed the Vi antigen stably. Vi antigen extracted from WR4103 was immunologically indistinguishable from Vi antigen purified from S. typhi Ty2. The only detectable difference between Ty21a and WR4103 was in the production of Vi antigen. The mean lethal doses of Ty21a and WR4103 for mice were nearly identical. Immunization of mice with WR4103 engendered a Vi antibody response and afforded complete protection against fatal infection with virulent S. typhi Ty2. Thus, S. typhi WR4103 may serve as an improved oral vaccine for protection against typhoid fever.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cryz SJ Jr,Fürer E,Baron LS,Noon KF,Rubin FA,Kopecko DJdoi
10.1128/IAI.57.12.3863-3868.1989subject
Has Abstractpub_date
1989-12-01 00:00:00pages
3863-8issue
12eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::The identification of genes with increased expression in vivo may lead to the identification of novel or unrecognized virulence traits and/or recognition of environmental signals involved in modulating gene expression. Our laboratory is studying an extraintestinal isolate of Escherichia coli as a model pathogen. We ha...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.60.6.2493-2499.1992
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.63.9.3550-3554.1995
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journal_title:Infection and immunity
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doi:10.1128/IAI.54.1.262-264.1986
更新日期:1986-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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journal_title:Infection and immunity
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doi:10.1128/IAI.66.3.1167-1173.1998
更新日期:1998-03-01 00:00:00
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doi:10.1128/iai.72.2.1029-1035.2004
更新日期:2004-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1246-1252.1995
更新日期:1995-04-01 00:00:00
abstract::The purpose of these experiments was to study the early response of mouse foot pads to Mycobacterium leprae. To accomplish this, mice were inoculated in both foot pads with large and small numbers of organisms. The animals were sacrificed at intervals from 2 hr to 27 days after inoculation. The microscopical results, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.1.76-85.1973
更新日期:1973-01-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.55.5.1051-1057.1987
更新日期:1987-05-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3705-3710.1995
更新日期:1995-09-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.32.2.681-689.1981
更新日期:1981-05-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/iai.72.2.949-957.2004
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更新日期:2017-08-18 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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