Scanning electron microscopic study of virulent and avirulent colonies of Neisseria gonorrhoeae.

Abstract:

:A preparation procedure for scanning electron microscopy was developed by which gononcoccal colonies can be studied directly on the agar surface. After glutaraldehyde fixation directly in petri dishes, small agar pieces were cut out and dehydrated stepwise in increasing concentrations of ethanol. The blocks were thereafter transferred to a critical-point drying apparatus, via steps of increased gradients up to 100% amyl acetate. By this method five different gonococcal colony types could be distinguished analogous to light microscopic observations made by others. At higher magnifications an abundance of intercellular strands was found between the cells in virulent type 1 and 2 colonies, but not in the avirulent types 3 through 5. These strands seemed to anchor the cells to each other and to the agar surface. The presence of such structures probably explains the highly convex surface of virulent colonies and explains why colonies of avirulent strains exhibit a radial extension and a flat upper surface. The nature of these filamentous intercellular strands is discussed.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Elmros T,Hörstedt P,Winblad B

doi

10.1128/IAI.12.3.630-637.1975

subject

Has Abstract

pub_date

1975-09-01 00:00:00

pages

630-7

issue

3

eissn

0019-9567

issn

1098-5522

journal_volume

12

pub_type

杂志文章
  • Biological and chemical characterization of toxic substances from Candida albicans.

    abstract::Whole cells, morphological components, and various extracts of Candida albicans were tested for toxicity by methods involving biological activities ordinarily used to characterize bacterial endotoxin. Fungal preparations exerted several of these activities, but only at much higher dose levels than those required for b...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.6.4.616-627.1972

    authors: Cutler JE,Friedman L,Milner KC

    更新日期:1972-10-01 00:00:00

  • Suppression of the intestinal immune response to cholera toxin by specific serum antibody.

    abstract::The possibility that preexisting specific serum antibody could suppress a defined mucosal immune response to a topically applied antigen was studied in rats. Hyperimmune serum antibody induced by parenteral immunization of rats with cholera toxoid markedly suppressed the mucosal immune response to enterically applied ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.30.1.62-68.1980

    authors: Pierce NF

    更新日期:1980-10-01 00:00:00

  • Local Induction of B Cell Interleukin-10 Competency Alleviates Inflammation and Bone Loss in Ligature-Induced Experimental Periodontitis in Mice.

    abstract::Interleukin-10 (IL-10)-producing B cells (B10 cells) play a critical role in the immune system balance by negatively regulating inflammatory responses. This study was conducted to determine the effect of local B10 cell induction on periodontal inflammation and bone loss in ligature-induced experimental periodontitis i...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00645-16

    authors: Yu P,Hu Y,Liu Z,Kawai T,Taubman MA,Li W,Han X

    更新日期:2016-12-29 00:00:00

  • Identification and characterization of a surface-associated protein (Ssp) of Staphylococcus saprophyticus.

    abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.3.1055-1060.1992

    authors: Gatermann S,Kreft B,Marre R,Wanner G

    更新日期:1992-03-01 00:00:00

  • Molecular chimerization of Pasteurella haemolytica leukotoxin to interleukin-2: effects on cytokine and antigen function.

    abstract::A chimeric recombinant protein composed of the lktA gene product from Pasteurella haemolytica fused to bovine interleukin-2 (IL-2) was made. The LKT-IL-2 chimera was compared with recombinant bovine IL-2 with regard to the ability to induce proliferative responses and LAK cell activity in bovine peripheral blood monon...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.2.565-570.1992

    authors: Hughes HP,Campos M,Potter AA,Babiuk LA

    更新日期:1992-02-01 00:00:00

  • espC pathogenicity island of enteropathogenic Escherichia coli encodes an enterotoxin.

    abstract::At least five proteins are secreted extracellularly by enteropathogenic Escherichia coli (EPEC), a leading cause of infant diarrhea in developing countries. However only one, EspC, is known to be secreted independently of the type III secretion apparatus encoded by genes located within the 35.6-kb locus of enterocyte ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.1.315-324.2001

    authors: Mellies JL,Navarro-Garcia F,Okeke I,Frederickson J,Nataro JP,Kaper JB

    更新日期:2001-01-01 00:00:00

  • Transmembrane tumor necrosis factor alpha is required for enteropathy and is sufficient to promote parasite expulsion in gastrointestinal helminth infection.

    abstract::To study the specific role of transmembrane tumor necrosis factor (tmTNF) in protective and pathological responses against the gastrointestinal helminth Trichinella spiralis, we compared the immune responses of TNF-alpha/lymphotoxin alpha (LTalpha)(-/-) mice expressing noncleavable transgenic tmTNF to those of TNF-alp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01461-08

    authors: Ierna MX,Scales HE,Mueller C,Lawrence CE

    更新日期:2009-09-01 00:00:00

  • Toll-Like Receptors 2 and 4 Modulate Pulmonary Inflammation and Host Factors Mediated by Outer Membrane Vesicles Derived from Acinetobacter baumannii.

    abstract::Pneumonia due to Gram-negative bacteria is associated with high mortality. Acinetobacter baumannii is a Gram-negative bacterium that is associated with hospital-acquired and ventilator-associated pneumonia. Bacteria have been described to release outer membrane vesicles (OMVs) that are capable of mediating systemic in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00243-19

    authors: Marion CR,Lee J,Sharma L,Park KS,Lee C,Liu W,Liu P,Feng J,Gho YS,Dela Cruz CS

    更新日期:2019-08-21 00:00:00

  • Paracoccidioides brasiliensis-stimulated human gamma/delta T cells support antibody production by B cells.

    abstract::Paracoccidioidomycosis patients show hyperactive humoral immune responses. Consequently, we investigated whether cytokines in supernatants from Paracoccidioides brasiliensis-stimulated gamma/delta T cells support B-cell activation. We detected proliferation of B cells and increased immunoglobulin M (IgM) and IgG produ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.4.1608-1610.1995

    authors: Munk ME,Fazioli RA,Calich VL,Kaufmann SH

    更新日期:1995-04-01 00:00:00

  • Safety and shedding of an attenuated strain of Listeria monocytogenes with a deletion of actA/plcB in adult volunteers: a dose escalation study of oral inoculation.

    abstract::Listeria monocytogenes is an intracellular bacterial pathogen which causes bacteremia and has a tropism for the central nervous system and a propensity to cause maternofetal infection. L. monocytogenes has been shown to be an effective prophylactic and a therapeutic vaccine vector for viral and tumor antigens in anima...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.7.3592-3601.2002

    authors: Angelakopoulos H,Loock K,Sisul DM,Jensen ER,Miller JF,Hohmann EL

    更新日期:2002-07-01 00:00:00

  • Effect of oral immunization with recombinant urease on murine Helicobacter felis gastritis.

    abstract::The ability of oral immunization to interfere with the establishment of infection with Helicobacter felis was examined. Groups of Swiss Webster mice were immunized orally with 250 micrograms of Helicobacter pylori recombinant urease (rUrease) and 10 micrograms of cholera toxin (CT) adjuvant, 1 mg of H. felis sonicate ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.4.1246-1252.1995

    authors: Pappo J,Thomas WD Jr,Kabok Z,Taylor NS,Murphy JC,Fox JG

    更新日期:1995-04-01 00:00:00

  • The major subunit, CfaB, of colonization factor antigen i from enterotoxigenic Escherichia coli is a glycosphingolipid binding protein.

    abstract::Bacterial adherence to mucosal surfaces is an important virulence trait of pathogenic bacteria. Adhesion of enterotoxigenic Escherichia coli (ETEC) to the intestine is mediated by a number of antigenically distinct colonization factors (CFs). One of the most common CFs is CFA/I. This has a fimbrial structure composed ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02006-05

    authors: Jansson L,Tobias J,Lebens M,Svennerholm AM,Teneberg S

    更新日期:2006-06-01 00:00:00

  • Experimental otitis media after nasal inoculation of Streptococcus pneumoniae and influenza A virus in chinchillas.

    abstract::Otitis media developed in 67% of chinchillas inoculated intranasally with type 7 Streptococcus pneumoniae and influenza A virus. Only 4% of chinchillas inoculated with influenza alone and 21% of chinchillas inoculated with S. pneumoniae alone developed otitis media. Among the chinchillas that developed otitis media af...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:

    authors: Giebink GS,Berzins IK,Marker SC,Schiffman G

    更新日期:1980-11-01 00:00:00

  • Identification of larval cross-reactive and egg-specific antigens involved in granuloma formation in murine schistosomiasis mansoni.

    abstract::Cross-reactive humoral immune responses between antigens of different developmental stages of the worm Schistosoma mansoni have previously been demonstrated. In contrast, information on antigenic cross-reactivity at the T-cell level is still very sparse. The present study examined the cross-reactive T-cell responses t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.9.3237-3242.1991

    authors: Lukacs NW,Boros DL

    更新日期:1991-09-01 00:00:00

  • Rapid protection of gnotobiotic pigs against experimental salmonellosis following induction of polymorphonuclear leukocytes by avirulent Salmonella enterica.

    abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.4.2182-2191.2003

    authors: Foster N,Lovell MA,Marston KL,Hulme SD,Frost AJ,Bland P,Barrow PA

    更新日期:2003-04-01 00:00:00

  • A new restriction fragment length polymorphism from Cryptosporidium parvum identifies genetically heterogeneous parasite populations and genotypic changes following transmission from bovine to human hosts.

    abstract::Length and restriction site polymorphism within a 2.8-kb threonine-rich open reading frame from Cryptosporidium parvum was identified and used to determine the genotypes of isolates from calves and humans. In agreement with observations of other genetic loci, all calf isolates were identical at this locus. In contrast...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.9.3958-3960.1997

    authors: Carraway M,Tzipori S,Widmer G

    更新日期:1997-09-01 00:00:00

  • In vivo expression and variation of Escherichia coli type 1 and P pili in the urine of adults with acute urinary tract infections.

    abstract::In vivo expression of pili by Escherichia coli in the urine of 41 adults with lower urinary tract infections was analyzed by immunostaining with polyclonal antiserum to type 1 and P pili. Type 1 pili were detected in 31 of 41 urine specimens, while P pili were detected in 6 of 18 specimens. The piliation status of bac...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.6.1656-1662.1989

    authors: Kisielius PV,Schwan WR,Amundsen SK,Duncan JL,Schaeffer AJ

    更新日期:1989-06-01 00:00:00

  • Effect of Trichinella spiralis infection on passive cutaneous anaphylaxis in mice.

    abstract::Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies, but was more pronou...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.15.1.84-90.1977

    authors: Munoz JJ,Cole RL

    更新日期:1977-01-01 00:00:00

  • Human antibodies against a purified glucosylceramide from Cryptococcus neoformans inhibit cell budding and fungal growth.

    abstract::A major ceramide monohexoside (CMH) was purified from lipidic extracts of Cryptococcus neoformans. This molecule was analyzed by high-performance thin-layer chromatography (HPTLC), gas chromatography coupled with mass spectrometry, and fast atom bombardment-mass spectrometry. The cryptococcal CMH is a beta-glucosylcer...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.12.7049-7060.2000

    authors: Rodrigues ML,Travassos LR,Miranda KR,Franzen AJ,Rozental S,de Souza W,Alviano CS,Barreto-Bergter E

    更新日期:2000-12-01 00:00:00

  • Inflammatory response following intranasal infection with Mycobacterium avium complex: role of T-cell subsets and gamma interferon.

    abstract::The role of CD4+ and CD8+ T cells in the response to intranasal infection with a Mycobacterium avium complex isolate (MAC) was investigated. Depletion of CD4+ T cells by injected antibody exacerbated infection in the lung, spleen, and liver. There were decreased numbers of inflammatory cells in the lungs of CD4-deplet...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.6.2282-2287.1995

    authors: Saunders BM,Cheers C

    更新日期:1995-06-01 00:00:00

  • Helicobacter pylori induces interleukin-8 secretion by Toll-like receptor 2- and Toll-like receptor 5-dependent and -independent pathways.

    abstract::Helicobacter pylori is an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. In response to H. pylori, interleukin-8 (IL-8) is secreted from host cells to attract components of the innate and adaptive ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.3.1523-1531.2005

    authors: Torok AM,Bouton AH,Goldberg JB

    更新日期:2005-03-01 00:00:00

  • Biochemical and biological characterization of the protective Leishmania pifanoi amastigote antigen P-8.

    abstract::The Leishmania pifanoi amastigote antigen P-8 has been previously shown to induce protective immunity in a murine model of cutaneous leishmaniasis (L. Soong, S. M. Duboise, P. Kima, and D. McMahon-Pratt, Infect. Immun. 63:3559-3566, 1995). As this antigen is of interest for further vaccine studies, the biochemical cha...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.11.6776-6784.2001

    authors: Colmenares M,Tiemeyer M,Kima P,McMahon-Pratt D

    更新日期:2001-11-01 00:00:00

  • Direct continuous method for monitoring biofilm infection in a mouse model.

    abstract::We have developed a rapid, continuous method for real-time monitoring of biofilms, both in vitro and in a mouse infection model, through noninvasive imaging of bioluminescent bacteria colonized on Teflon catheters. Two important biofilm-forming bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, wer...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.2.882-890.2003

    authors: Kadurugamuwa JL,Sin L,Albert E,Yu J,Francis K,DeBoer M,Rubin M,Bellinger-Kawahara C,Parr TR Jr,Contag PR

    更新日期:2003-02-01 00:00:00

  • Demonstration of lipopolysaccharide with O-polysaccharide chains among different heat-stable serotypes of Campylobacter jejuni by silver staining of polyacrylamide gels.

    abstract::Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family E...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.12.5384-5387.1993

    authors: Blake DC Jr,Russell RG

    更新日期:1993-12-01 00:00:00

  • Experimental Escherichia coli ascending pyelonephritis in rats: active peroral immunization with live Escherichia coli.

    abstract::Peroral immunization with a live strain of Escherichia coli O6K13H1 against experimental ascending pyelonephritis caused by the same strain was studied in rats, and the effect of immunization on antibody titers against the O and K antigens and lipid A was determined. Peroral immunization with live bacteria protected s...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.35.2.647-653.1982

    authors: Mattsby-Baltzer I,Hanson LA,Olling S,Kaijser B

    更新日期:1982-02-01 00:00:00

  • Staphylococcus aureus serotype 5 capsular polysaccharide is antiphagocytic and enhances bacterial virulence in a murine bacteremia model.

    abstract::Controversy persists over the role that the capsular polysaccharide plays in the pathogenesis of Staphylococcus aureus infections. To address this issue, we compared the mouse virulence of S. aureus Reynolds and capsule-defective mutant strains cultivated under conditions of high or low capsule expression. Strain Reyn...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.11.5183-5189.1998

    authors: Thakker M,Park JS,Carey V,Lee JC

    更新日期:1998-11-01 00:00:00

  • Plasmodium falciparum line-dependent association of in vitro growth-inhibitory activity and risk of malaria.

    abstract::Plasmodium falciparum's ability to invade erythrocytes is essential for its survival within the human host. Immune mechanisms that impair this ability are therefore expected to contribute to immunity against the parasite. Plasma of humans who are naturally exposed to malaria has been shown to have growth-inhibitory ac...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.06190-11

    authors: Rono J,Färnert A,Olsson D,Osier F,Rooth I,Persson KE

    更新日期:2012-05-01 00:00:00

  • In vitro sensitivity of oral, gram-negative, facultative bacteria to the bactericidal activity of human neutrophil defensins.

    abstract::Neutrophils play a major role in defending the periodontium against infection by oral, gram-negative, facultative bacteria, such as Actinobacillus actinomycetemcomitans, Eikenella corrodens, and Capnocytophaga spp. We examined the sensitivity of these bacteria to a mixture of low-molecular-weight peptides and highly p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.12.3934-3940.1990

    authors: Miyasaki KT,Bodeau AL,Ganz T,Selsted ME,Lehrer RI

    更新日期:1990-12-01 00:00:00

  • Levels of inhibitors of tumor necrosis factor alpha and interleukin 1beta in urine and sera of patients with urosepsis.

    abstract::The antiinflammatory cytokine response during urosepsis was determined by measurement of concentrations of soluble tumor necrosis factor receptor (sTNFR) types I and II, interleukin 1 receptor antagonist (IL-1ra), soluble IL-1 receptor type II (sIL-1RII), and interleukin 10 in sera and urine of 30 patients with cultur...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.8.3527-3534.1998

    authors: Olszyna DP,Prins JM,Buis B,van Deventer SJ,Speelman P,van der Poll T

    更新日期:1998-08-01 00:00:00

  • Crossed immunoelectrophoretic analysis of anti-Salmonella typhi antibodies in sera of typhoid patients and carriers: demonstration of the presence of typhoid-specific antibodies to a non-O, non-H, non-Vi antigen.

    abstract::A veronal buffer extract of Salmonella typhi was used as the reference antigen and its corresponding rabbit antiserum as the reference antibody in crossed immunoelectrophoresis to analyze antibodies in sera obtained from typhoid patients and carriers. Four precipitating antibodies were regularly detected. Three were a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.43.3.1110-1113.1984

    authors: Chau PY,Wan KC,Tsang RS

    更新日期:1984-03-01 00:00:00