Abstract:
:Interleukin-10 (IL-10)-producing B cells (B10 cells) play a critical role in the immune system balance by negatively regulating inflammatory responses. This study was conducted to determine the effect of local B10 cell induction on periodontal inflammation and bone loss in ligature-induced experimental periodontitis in vivo Purified spleen B cells from C57BL/6J mice (8 to 10 weeks old) were cultured with CD40 ligand (CD40L) and the Toll-like receptor 9 (TLR9) agonist cytidine-phosphate-guanosine oligodeoxynucleotide (CpG) to determine effective IL-10 induction in vitro Silk ligatures (size 7-0) were tied around the mouse maxillary second molars on day 0, followed by the injection of CD40L and CpG into the palatal gingiva on days 3, 6, and 9. All the mice were sacrificed, and samples were collected on day 14. CD40L and CpG significantly increased the level of IL-10 production by B cells in vitro, although the frequencies of CD1dhi CD5+ and IL-10-producing (IL-10+) CD45+ cells were decreased. IL-10 was predominantly produced by the CD1dhi CD5+ subpopulation of B cells. In vivo, both IL-10 mRNA expression and the number of IL-10+ CD45+ cells were significantly increased after gingival injection of CD40L and CpG. Periodontal bone loss was significantly decreased and the gingival expression of IL-1β, tumor necrosis factor alpha, and RANKL was significantly reduced. The number of multinucleated tartrate-resistant acid phosphatase-positive cells along the alveolar bone surface was significantly decreased after gingival injection of CD40L and CpG. This study indicates for the first time that the local induction of B10 cell activity could inhibit periodontal inflammation and bone loss.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Yu P,Hu Y,Liu Z,Kawai T,Taubman MA,Li W,Han Xdoi
10.1128/IAI.00645-16subject
Has Abstractpub_date
2016-12-29 00:00:00issue
1eissn
0019-9567issn
1098-5522pii
IAI.00645-16journal_volume
85pub_type
杂志文章abstract::The adhesion of gram-positive bacteria to extracellular matrix (ECM) proteins is regarded as an important determinant of pathogenicity. A comparison of the adhesion of Streptococcus agalactiae strain O90R to different ECM proteins showed that the most pronounced binding could be observed for immobilized fibrinogen. To...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2434-2440.2002
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5044-5048.1993
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.800-808.1982
更新日期:1982-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.971-977.1981
更新日期:1981-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1043-1047.1990
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journal_title:Infection and immunity
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pub_type: 杂志文章
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journal_title:Infection and immunity
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doi:10.1128/IAI.13.2.438-447.1976
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00088-19
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.58.12.3988-3995.1990
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.202-210.1979
更新日期:1979-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.00943-08
更新日期:2009-01-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.62.5.1776-1786.1994
更新日期:1994-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.2892-2898.1995
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1038-1044.1975
更新日期:1975-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.320-328.1987
更新日期:1987-02-01 00:00:00
abstract::Human plasma was bactericidal for small numbers of Klebsiella pneumoniae, but larger numbers grew slowly over a period of 24 h. Human polymorphs in a clot of autologous plasma had a bacteriostatic effect on relatively large numbers of bacteria for up to 24 h and were much more effective than plasma alone. The bacteric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.723-730.1986
更新日期:1986-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3588-3593.1990
更新日期:1990-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2535-2541.1990
更新日期:1990-08-01 00:00:00
abstract::The levels of protection found in vaccine studies of murine visceral leishmaniasis are significantly lower than for cutaneous leishmaniasis; whether this is due to the high-challenge murine model employed and/or is a consequence of differences required in tissue-specific local immune responses is not understood. Conse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.401-410.2003
更新日期:2003-01-01 00:00:00
abstract::The production and role of tumor necrosis factor alpha (TNF-alpha) in pneumococcal pneumonia were investigated in a mouse pneumonia model. When approximately 10(6) CFU of Streptococcus pneumoniae TUM19 were used to inoculate CBA/J mice intranasally, TNF-alpha levels in the lungs and serum began to increase from 1 and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.257-260.1997
更新日期:1997-01-01 00:00:00