Abstract:
:The human pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 has two histidine sensor kinases, QseC and QseE, which respond to the mammalian adrenergic hormones epinephrine and norepinephrine by increasing their autophosphorylation. Although QseC and QseE are present in nonpathogenic strains of E. coli, EHEC exploits these kinases for virulence regulation. To further investigate the full extent of epinephrine and its sensors' impact on EHEC virulence, we performed transcriptomic and phenotypic analyses of single and double deletions of qseC and qseE genes in the absence or presence of epinephrine. We showed that in EHEC, epinephrine sensing seems to occur primarily through QseC and QseE. We also observed that QseC and QseE regulate expression of the locus of enterocyte effacement (LEE) genes positively and negatively, respectively. LEE activation, which is required for the formation of the characteristic attaching and effacing (A/E) lesions by EHEC on epithelial cells, is epinephrine dependent. Regulation of the LEE and the non-LEE-contained virulence factor gene nleA by QseE is indirect, through transcription inhibition of the RcsB response regulator. Finally, we show that coincubation of HeLa cells with epinephrine increases EHEC infectivity in a QseC- and QseE-dependent manner. These results genetically and phenotypically map the contributions of the two adrenergic sensors QseC and QseE to EHEC pathogenesis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Njoroge J,Sperandio Vdoi
10.1128/IAI.05921-11subject
Has Abstractpub_date
2012-02-01 00:00:00pages
688-703issue
2eissn
0019-9567issn
1098-5522pii
IAI.05921-11journal_volume
80pub_type
杂志文章abstract::Immune responses of mice to Streptococcus mutans serotype c were analyzed by means of the enzyme-linked immunospot assay to determine the predominant specificities of the antibodies developed. In general, the numbers of splenic antibody-secreting cells correlated with serum antibody levels. A low dose (10(8) CFU) of k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.317-323.1986
更新日期:1986-08-01 00:00:00
abstract::In this study we tested the in vitro and in vivo anti-Cryptococcus neoformans activity of an antilaminarin (anti-beta-glucan) monoclonal antibody (MAb 2G8) (immunoglobulin G2b) which was previously shown to inhibit the growth of beta-glucan-exposing Candida albicans cells. Here we show that MAb 2G8 binds to the cell w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00278-07
更新日期:2007-11-01 00:00:00
abstract::The bacterium Neisseria meningitidis is the causative agent of meningitis and sepsis. A generally effective vaccine against N. meningitidis serogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion of N. meningitidis wild-ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00846-07
更新日期:2007-12-01 00:00:00
abstract::Heterogeneic autoantibodies against axonal neurofilament proteins of mature mouse neurons grown in vitro were detected by the indirect immunofluorescence technique in 12.7% (9 of 71) of the sera from nonhuman primates infected with kuru, in 14.5% (17 of 117) and 4% (1 of 25), respectively, of the sera from nonhuman pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.316-324.1982
更新日期:1982-10-01 00:00:00
abstract::We investigated the ability of interleukin 10 (IL-10) to protect mice against lethal shock induced by staphylococcal enterotoxin B (SEB). Treatment of mice with IL-10 prevented the death of mice injected with SEB in a dose-dependent manner. IL-10-mediated protection was apparent when administered either prior to or co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4937-4939.1993
更新日期:1993-11-01 00:00:00
abstract::Pertussis is a severe respiratory disease caused by Bordetella pertussis The classic symptoms of pertussis include paroxysmal coughing with an inspiratory whoop, posttussive vomiting, cyanosis, and persistent coryzal symptoms. Infants under 2 months of age experience more severe disease, with most deaths occurring in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00511-18
更新日期:2018-10-25 00:00:00
abstract::Cell wall proteins with sialidase activity are involved in carbohydrate assimilation, adhesion to mucosal surfaces, and biofilm formation. Gardnerella spp. inhabit the human vaginal microbiome and encode up to three sialidase enzymes, two of which are suspected to be cell wall associated. Here we demonstrate that the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00583-20
更新日期:2020-12-23 00:00:00
abstract::The effect of a standard regimen of cimetidine on the gastric flora of 20 male volunteers was studied in a double-blind manner and compared with the effects of a standard antacid regimen. Postprandial microbial titers in gastric aspirates were significantly higher at 4, 8, and 16 weeks of therapy in subjects taking an...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.36.2.518-524.1982
更新日期:1982-05-01 00:00:00
abstract::The literature on chronic mucocutaneous candidiasis contains multiple reports which suggest that loss of cell-mediated immunity in this disease may be related in part to the presence of an inhibitory factor(s) present in patient plasma. One such inhibitory factor has been suggested to be mannan polysaccharide released...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1041-1046.1984
更新日期:1984-03-01 00:00:00
abstract::To evaluate the enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides, the rate of isolation of these organisms was compared among individuals with and without diarrhea in Thailand. In two groups of American travelers, A. hydrophila, but not P. shigelloides, was associated with episodes of travelers...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.666-673.1982
更新日期:1982-02-01 00:00:00
abstract::Antisera were produced by inoculation of mycoplasma cells grown in PPLO broth supplemented with rabbit serum alone or rabbit serum plus Freund complete or incomplete adjuvant. By using an enzyme-linked immunosorbent assay, immunoglobulin G antibodies to cholesterol were detected in antisera to mycoplasmas (Mycoplasma ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.2200-2202.1991
更新日期:1991-06-01 00:00:00
abstract::Citrobacter rodentium, a murine model pathogen for enteropathogenic Escherichia coli, colonizes the surface of intestinal epithelial cells and causes mucosal inflammation. This bacterium is an ideal model for investigating pathogen-host immune interactions in the gut. It is well known that gene transcripts for Th1 cyt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01343-09
更新日期:2010-06-01 00:00:00
abstract::We show in this study that toxin production in Clostridium difficile is altered in cells which can no longer form flagellar filaments. The impact of inactivation of fliC, CD0240, fliF, fliG, fliM, and flhB-fliR flagellar genes upon toxin levels in culture supernatants was assessed using cell-based cytotoxicity assay, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-12
更新日期:2012-10-01 00:00:00
abstract::Trichomonas vaginalis infected with a double-stranded RNA virus undergoes phenotypic variation on the basis of surface versus cytoplasmic expression of the immunogenic protein P270. Examination of batch cultures by flow cytofluorometry with monoclonal antibody (MAb) to P270 yields both fluorescent and nonfluorescent t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4298-4302.1999
更新日期:1999-08-01 00:00:00
abstract::The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.371-376.1994
更新日期:1994-02-01 00:00:00
abstract::Colonization of the large bowel of healthy infants by Clostridium difficile was studied. Feces were collected from five breast-fed aand five formula-fed infants throughout the first year of life, and levels of C. difficile were quantitated. Three breast-fed and five formula-fed infants were colonized for periods of be...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.895-899.1982
更新日期:1982-03-01 00:00:00
abstract::The effects of tolerance to Escherichia coli endotoxin on the phagocytic and bactericidal activity of the hepatic reticuloendothelial system against viable E. coli were examined using ex vivo perfused rat livers. Livers were isolated from control and endotoxin-tolerant rats and perfused with a medium containing 5% hom...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.798-803.1980
更新日期:1980-03-01 00:00:00
abstract::Synthetic lipid A analogs which have two amide-bound and two ester-bound (R)-3-hydroxytetradecanoyl groups at the C-2 and -2' and C-3 and -3' positions of beta(1-6)glucosamine disaccharide mono- or diphosphates showed high activities in most in vitro assays, and the lethality of a diphosphate derivative to galactosami...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.293-296.1984
更新日期:1984-07-01 00:00:00
abstract::Recent reports have suggested that oral vaccination of mice against Helicobacter pylori is dependent on a Th1-mediated immune response. However, oral vaccination in mice neither induces sterilizing immunity nor leads to complete protection from disease. Therefore, in this study we investigated whether a systemic subcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1029-1035.2004
更新日期:2004-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.1.80-86.1971
更新日期:1971-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3033-3041.1992
更新日期:1992-08-01 00:00:00
abstract::Various invasive pathogens attach to host tissues via the extracellular matrix component laminin, the major glycoprotein found within basement membranes. Previous investigations identified the laminin-binding adhesin Tp0751 within the spirochete bacterium Treponema pallidum. In the current study, Tp0751 was shown to a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7485-7494.2005
更新日期:2005-11-01 00:00:00
abstract::Increased protection against a virulent challenge with Mycobacterium tuberculosis is induced mainly by a previous immunization with living avirulent mycobacteria, usually Mycobacterium bovis BCG. Only a transient and marginal protection is obtained after immunization with bacterial extracts or dead bacteria. Both livi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.742-750.1993
更新日期:1993-02-01 00:00:00
abstract::Vibrio cholerae causes human infection through ingestion of contaminated food and water, leading to the devastating diarrheal disease cholera. V. cholerae forms matrix-encased aggregates, known as biofilms, in the native aquatic environment. While the formation of V. cholerae biofilms has been well studied, little is ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02617-14
更新日期:2015-01-01 00:00:00
abstract::Rabbit endogenous pyrogens were of about the same molecular size, but showed considerable heterogeneity of their isoelectric points. We attempted to show that this heterogeneity was attributable to variable glycosylation of a single polypeptide chain. When peritoneal exudate cells were stimulated to make pyrogens in t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.184-191.1981
更新日期:1981-10-01 00:00:00
abstract::Cytokine mRNA expression in biopsies of Mycobacterium ulcerans-infected human tissue was investigated using real-time PCR, and the findings were correlated with the clinical stages of disease and histopathologies. A broad range of cytokine mRNAs were detected in 16 early nodules and 28 late-stage ulcers, including tho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2917-2924.2006
更新日期:2006-05-01 00:00:00
abstract::Peptidoglycan from a Staphylococcus epidermidis strain, isolated from a patient with septicemia, was preincubated with human serum. This mixture was then investigated for its potency to induce tumor necrosis factor (TNF) secretion by human blood monocytes. TNF was measured in the supernatants by using a bioassay and/o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3837-3843.1994
更新日期:1994-09-01 00:00:00
abstract::The protozoan parasite Cryptosporidium parvum is a significant cause of diarrheal disease worldwide. Attachment to and invasion of host intestinal epithelial cells by C. parvum sporozoites are crucial steps in the pathogenesis of cryptosporidiosis. The molecular basis of these initial interactions is unknown. In order...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5167-5175.2000
更新日期:2000-09-01 00:00:00
abstract::Although the suckling mouse assay is widely used for the detection of heat-stable Escherichia coli enterotoxin (ST), few data have been published concerning the reproducibility, optimal growth, and test conditions of this assay. Four strains of toxigenic E. coli known to elaborate both heat-labile enterotoxin and ST o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.95-99.1976
更新日期:1976-07-01 00:00:00
abstract::Anopheles gambiae is the major African vector of Plasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00864-06
更新日期:2007-04-01 00:00:00