Abstract:
:Rabbit endogenous pyrogens were of about the same molecular size, but showed considerable heterogeneity of their isoelectric points. We attempted to show that this heterogeneity was attributable to variable glycosylation of a single polypeptide chain. When peritoneal exudate cells were stimulated to make pyrogens in the presence of 2-deoxy-D-glucose, there was a relatively trivial suppression of pyrogen release, and analysis by isoelectric focusing showed parallel inhibition of secretion of all the forms of endogenous pyrogen. When cells were stimulated in the presence of 3H-labeled amino acids and 14C-labeled glucosamine or glucose, the purified pyrogens were labeled with 3H but not with 14C. Macrophage membrane preparations were made which contained glycosyl transferases and could transfer sugar residues from sugar nucleotides to deglycosylated fetuin. These macrophage membrane preparations did not transfer sugars to the pI 7.3 endogenous pyrogen. Treatment of endogenous pyrogens with neuraminidase or with periodate produced no evidence suggesting that the pyrogens were glycosylated. Last, endogenous pyrogens did not bind to any of four lectins with different carbohydrate specificities. This evidence suggests that the heterogeneity of rabbit endogenous pyrogens is not attributable to glycosylation and must have some other cause.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Murphy PA,Cebula TA,Windle BEdoi
10.1128/IAI.34.1.184-191.1981subject
Has Abstractpub_date
1981-10-01 00:00:00pages
184-91issue
1eissn
0019-9567issn
1098-5522journal_volume
34pub_type
杂志文章abstract::Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.511-517.1984
更新日期:1984-08-01 00:00:00
abstract::Staphylococcus aureus is a pathogen often found in pneumonia and sepsis. In the context of the resistance of this organism to conventional antibiotics, an understanding of the regulation of natural endogenous antimicrobial molecules is of paramount importance. Previous studies have shown that both human and mouse airw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3609-3617.2005
更新日期:2005-06-01 00:00:00
abstract::Although protective immunity in C57BL/6 mice induced by a single dose of the radiation-attenuated schistosome vaccine is believed to be mediated by Th1-type immune responses, we here report that in BALB/c mice protection can also depend upon signaling via the interleukin-4 (IL-4) receptor which conventionally governs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.228-236.2001
更新日期:2001-01-01 00:00:00
abstract::Salmonella enterica serovar Typhi is a human-restricted Gram-negative bacterial pathogen responsible for causing an estimated 27 million cases of typhoid fever annually, leading to 217,000 deaths, and current vaccines do not offer full protection. The O-antigen side chain of the lipopolysaccharide is an immunodominant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01021-16
更新日期:2017-03-23 00:00:00
abstract::Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00692-19
更新日期:2019-11-18 00:00:00
abstract::Protective immunity against Babesia bigemina is hypothesized to involve antibodies directed against merozoite surface-exposed epitopes. Levels of antibody against a rhoptry-associated protein 1 (RAP-1) B-lymphocyte epitope, defined by surface-reactive and inhibitory monoclonal antibodies, in immune cattle sera were de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5698-5701.1994
更新日期:1994-12-01 00:00:00
abstract::Chemiluminescence of human alveolar macrophages (AM) was evaluated in vitro. Unstimulated AM generated chemiluminescence that remained constant during incubation. Addition of heat-killed Staphylococcus aureus 502A (HKB) or a chemical agent, phorbol myristate acetate, produced high rates of chemiluminescence that were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.1.117-120.1977
更新日期:1977-07-01 00:00:00
abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.1106-1108.1976
更新日期:1976-10-01 00:00:00
abstract::Regulation of the synthesis of Vi polysaccharide, a major virulence determinant in Salmonella enterica serotype Typhi, is under the control of two regulatory systems, ompR-envZ and rscB-rscC, which respond to changes in osmolarity. Some serotype Typhi strains exhibit overexpression of Vi polysaccharide, which masks cl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00888-06
更新日期:2007-03-01 00:00:00
abstract::Mosquitoes are infected with Plasmodium falciparum by taking a blood meal from a gametocyte carrier. Since a mosquito takes a volume of 1 to 2 microl, a blood meal may contain 1 x 10(4) to 3 x 10(4) leukocytes (WBC). The majority of WBC are composed of neutrophils which may phagocytose and kill developing gametes insi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3834-3837.1997
更新日期:1997-09-01 00:00:00
abstract::In nature, mixed Borrelia burgdorferi infections are common and possibly can be acquired by either superinfection or coinfection. Superinfection by heterologous B. burgdorferi strains has been established experimentally, although the ability of homologous B. burgdorferi clones to superinfect a host has not been studie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01817-14
更新日期:2014-11-01 00:00:00
abstract::Depressed Th1 responses are a prominent feature of human tuberculosis, but an enhanced Th2 response has not been detected in peripheral blood T cells stimulated in vitro with Mycobacterium tuberculosis. In disease due to Mycobacterium leprae, Th2 cells predominate in tissue lesions of patients with extensive disease b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1351-1356.1996
更新日期:1996-04-01 00:00:00
abstract::Shigella flexneri 2a is an invasive enteric pathogen that may produce diarrhea when ingested by human beings and subhuman primates. We have previously shown that shigella diarrhea correlates with water and electrolyte transport abnormalities in the jejunum and colon. Dysentery alone is associated only with colonic tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.368-371.1976
更新日期:1976-08-01 00:00:00
abstract::After uptake and retrograde transport pertussis toxin acts by ADP-ribosylating alpha-Gi proteins. We show that uptake via many different receptor proteins followed by retrograde transport and intoxication is not restricted to a particular cell type. The efficiency of cellular intoxication, however, was found to be cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.433-435.1999
更新日期:1999-01-01 00:00:00
abstract::We have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical Vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. Here, we cloned and sequenced the 15,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5742-5748.2000
更新日期:2000-10-01 00:00:00
abstract::The role of macrophages in protecting mice from murine cytomegalovirus (MCMV) was studied in Swiss, CBA/J, and C57BL/6J mice. CBA/J mice were more resistant to virus than were C57BL/6J mice at all ages tested. Prior treatment of adult Swiss mice with 60 mg of silica, a dose selectively toxic to macrophages, increased ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1383-1390.1974
更新日期:1974-12-01 00:00:00
abstract::Comparison of the susceptibility of outbred OF1 and inbred BALB/c, C57BL/6, DBA/2, and CBA mice to heat-stable toxin (STa) of enterotoxigenic Escherichia coli was made at different levels of induced secretion. STa was able to elicit fluid accumulation into the intestine of each strain of mice; however, quantitatively ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3117-3121.1992
更新日期:1992-08-01 00:00:00
abstract::A receptor moiety and blood group substance recognized by the O75X adhesin was studied. Well-defined erythrocytes representing different blood group systems and bacterial derivatives carrying plasmid pBJN406 encoding the adhesin were used in a direct hemagglutination assay. We showed that Dr blood group antigen, a com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1057-1060.1988
更新日期:1988-05-01 00:00:00
abstract::Legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 microgram of cytochalasin D per ml. Attachment was not inhibited by cytochalasin D. No extracellular multiplication occurred when L. pneumophila...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.31-36.1986
更新日期:1986-01-01 00:00:00
abstract::Organ cultures of adult hamster trachea were used to evaluate the cytotoxic potential of cell fractions of Mycoplasma pneumoniae. Cytoplasm was essentially devoid of activity, whereas viable cells and membrane preparations, at a level of 25 mug of protein per ml, induced necrosis. Damage, as revealed by light and elec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1127-1134.1974
更新日期:1974-11-01 00:00:00
abstract::Strain superinfection occurs when a second pathogen strain infects a host already infected with a primary strain. The selective pressures that drive strain divergence, which underlies superinfection, and allow penetration of a new strain into a host population are critical knowledge gaps relevant to shifts in infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02537-14
更新日期:2014-12-01 00:00:00
abstract::Staphylococcus aureus peptidoglycan displayed a marked dose-dependent mitogenic activity for mouse splenocytes and human peripheral blood lymphocytes in vitro, as measured by increased [3H]thymidine incorporation. Similarly it was mitogenic for athymic nude mouse spleen cells, whereas no blastogenic effect was observe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.3.706-710.1979
更新日期:1979-03-01 00:00:00
abstract::Ehrlichia chaffeensis is an obligately intracellular gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces the generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan. Bioinfomatic analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00056-08
更新日期:2008-08-01 00:00:00
abstract::Tissue macrophages are the obligatory host cells for Leishmania donovani, the causative agent of visceral leishmaniasis. In this study we sought to determine whether activated macrophages, as defined by the functional criterion of tumor cell cytotoxicity, were also able to exert a microbicidal effect on ingested L. do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.918-926.1981
更新日期:1981-09-01 00:00:00
abstract::Infection of inbred mouse strains with Borrelia burgdorferi results in the development of experimental Lyme arthritis. The degree of arthritic pathology has been suggested to correlate with the level of spirochete burden within tissues. To investigate this further, we infected resistant DBA/2 (DBA) and susceptible C3H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2065-2071.1998
更新日期:1998-05-01 00:00:00
abstract::Cutaneous leishmaniasis is characterized by vascular remodeling. Following infection with Leishmania parasites, the vascular endothelial growth factor A (VEGF-A)/VEGF receptor 2 (VEGFR-2) signaling pathway mediates lymphangiogenesis, which is critical for lesion resolution. Therefore, we investigated the cellular and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00088-19
更新日期:2019-10-18 00:00:00
abstract::Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01635-07
更新日期:2008-04-01 00:00:00
abstract::Helicobacter pylori mutants deficient in 6-phosphogluconate dehydratase (6PGD) were constructed. Colonization densities were lower and minimum infectious doses were higher for mutant strains than for wild-type strains. In spite of better colonization, however, wild-type strains did not displace the mutant in cocoloniz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2920-2923.2003
更新日期:2003-05-01 00:00:00
abstract::In this study, we investigated a potential requirement of two-component signal transduction systems for acid resistance in Helicobacter pylori. In comparison to a wild-type strain, isogenic strains with null mutations in either HP0165 or HP1364 histidine kinases were impaired in their ability to grow at pH 5.0. The gr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.3052-3059.2006
更新日期:2006-05-01 00:00:00
abstract::Rickettsia rickettsii infection of endothelial cells is manifested in very distinctive changes in cell morphology, consisting of extensive dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope and blebbing of the plasma membrane, as seen by transmission electron microscopy (D. J. Silverma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2290-2299.1998
更新日期:1998-05-01 00:00:00