Abstract:
:The literature on chronic mucocutaneous candidiasis contains multiple reports which suggest that loss of cell-mediated immunity in this disease may be related in part to the presence of an inhibitory factor(s) present in patient plasma. One such inhibitory factor has been suggested to be mannan polysaccharide released from the cell wall of the pathogen. The present report describes results of experiments to consider mechanisms by which yeast mannan influences proliferative responses of human lymphocytes. Mannan for these experiments was isolated from Saccharomyces cerevisiae. We observed that mannan-mediated inhibition of proliferative responses to a battery of stimuli (phytohemagglutinin, pokeweed mitogen, and Candida, mumps, streptococcus, cytomegalovirus, and herpes simplex virus antigens) was related in part to an effect of copper associated with the mannan and possibly to the superoxide dismutase activity of the mannan-copper complex. Mannan made deficient in copper by use of a copper-chelating resin appeared to inhibit only lymphoproliferation stimulated by the Candida antigen. These results suggest that inhibitory effects of yeast mannans on lymphoproliferative responses may involve at least two mechanisms, one related to hydrogen peroxide production augmented by mannan-copper complexes and another related to still unknown effects independent of the metal ligand. We propose that our results represent a significant novel observation which may be useful in understanding mechanisms of immunoinhibitory effects of C. albicans mannan.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nelson RD,Herron MJ,McCormack RT,Gehrz RCdoi
10.1128/IAI.43.3.1041-1046.1984subject
Has Abstractpub_date
1984-03-01 00:00:00pages
1041-6issue
3eissn
0019-9567issn
1098-5522journal_volume
43pub_type
杂志文章abstract::The adherence mechanism of Aeromonas caviae Sch3N to HEp-2 cells was initially investigated through four mini-Tn5 mutants that showed a 10-fold decrease in adherence. These mutants lost motility, flagella, and their lipopolysaccharide (LPS) O antigen (O-Ag). Three genes, flmB-neuA-flmD, were found to be interrupted by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.65-74.2001
更新日期:2001-01-01 00:00:00
abstract::Acquired cellular immunity to infection with Mycobacterium tuberculosis is believed to reside in the capacity of mononuclear phagocytes of immunized animals to inhibit intracellular multiplication of the parasite. However, in macrophage tissue culture systems, it has been customary to employ streptomycin in the medium...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-01-01 00:00:00
abstract::Iron starvation conditions limited the growth of Salmonella typhi, as evidenced by an increase in the lag phase of a culture and a decrease in the number of bacteria reached in the stationary phase. The analysis of the outer membrane of bacteria grown under these conditions identified new protein components with appar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1271-1275.1989
更新日期:1989-04-01 00:00:00
abstract::Immunity to infection with Eimeria vermiformis was transferred in NIH mice by both the nylon wool-adherent (B-cell-enriched) and nonadherent (T-cell-enriched) fractions of lymphocytes (spleen and mesenteric lymph node) taken from infected donors. Transfer was more variable with the adherent fraction, and when contamin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1760-1765.1988
更新日期:1988-07-01 00:00:00
abstract::Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2416-2419.1987
更新日期:1987-10-01 00:00:00
abstract::While several diseases associated with Chlamydia psittaci infection have been reported in Phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. In this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1897-1900.1988
更新日期:1988-08-01 00:00:00
abstract::Bovine tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) cDNAs were generated by reverse transcription and then by PCR amplification from lipopolysaccharide (LPS)-stimulated alveolar macrophage RNA. The amplified cDNAs were cloned into pPow and expressed in Escherichia coli DH5 alpha. The expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.381-388.1995
更新日期:1995-02-01 00:00:00
abstract::Both interleukin-17A (IL-17A) and IL-17F are proinflammatory cytokines that have an important role in intestinal homeostasis via receptor signaling. These cytokines have been characterized in chickens, but very little is known about their receptors and their functional activity. We provide here the first description o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02141-14
更新日期:2014-09-01 00:00:00
abstract::The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T-cell and B-cell epitopes as the constituents of a single immunogen. Here we report on the design, chemical synthesis, and immunogenicity of three Plasmodium falciparum MAP vaccines that incorporated antig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-10
更新日期:2010-11-01 00:00:00
abstract::Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and chronic lung infections in cystic fibrosis patients. Iron is essential for bacterial growth, and P. aeruginosa expresses multiple iron uptake systems, whose role in lung infection deserves further investigation. P. aeruginosa Fe(3+) uptake sy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00098-16
更新日期:2016-07-21 00:00:00
abstract::The derivative toxins purified from cultures of proteolytic strains of Clostridium botulinum types A and F were found to have been only partially nicked but were fully activated. Trypsinization of C. botulinum type B derivative toxin at pH 6.0 resulted in simultaneous activation and nicking, whereas at pH 4.5, activat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.402-407.1977
更新日期:1977-08-01 00:00:00
abstract::Intestinal epithelial cells (IECs), including secretory goblet cells, form essential physiochemical barriers that separate luminal bacteria from underlying immune cells in the intestinal mucosa. IECs are common targets for enteric bacterial pathogens, with hosts responding to these microbes through innate toll-like re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02045-14
更新日期:2014-09-01 00:00:00
abstract::Helicobacter pylori mutants deficient in 6-phosphogluconate dehydratase (6PGD) were constructed. Colonization densities were lower and minimum infectious doses were higher for mutant strains than for wild-type strains. In spite of better colonization, however, wild-type strains did not displace the mutant in cocoloniz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2920-2923.2003
更新日期:2003-05-01 00:00:00
abstract::Mice immunized with a killed vaccine of phase I Bordetella bronchiseptica were challenged with various numbers of virulent B. bronchiseptica by intraperitoneal, intracerebral, or intranasal routes. The course of infection was compared among these routes, and the protective effect of vaccination was quantitatively anal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.2.598-603.1983
更新日期:1983-08-01 00:00:00
abstract::Inactivation of sortase gene srtA in Streptococcus pneumoniae strain R6 caused the release of beta-galactosidase and neuraminidase A (NanA) from the cell wall into the surrounding medium. Both of these surface proteins contain the LPXTG motif in the C-terminal domain. Complementation with plasmid-borne srtA reversed p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2758-2765.2003
更新日期:2003-05-01 00:00:00
abstract::A gene bank of group A Streptococcus strain Manfredo (M protein serotype 5) was constructed with a bacteriophage lambda vector-Escherichia coli K-12 host system and screened by immunoblotting hybrid phage plaques with antisera raised to purified pep M5 (serotype 5 M protein fragment released from the streptococcal cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.190-197.1985
更新日期:1985-04-01 00:00:00
abstract::Filifactor alocis is a recently recognized periodontal pathogen; however, little is known regarding its interactions with the immune system. As the first-responder phagocytic cells, neutrophils are recruited in large numbers to the periodontal pocket, where they play a crucial role in the innate defense of the periodo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00496-16
更新日期:2016-11-18 00:00:00
abstract::Two virulence factors of Listeria monocytogenes, listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC), mediate escape of this pathogen from the phagocytic vacuole of macrophages, thereby allowing the bacterium access to the host cell cytosol for growth and spread to neighboring cells. We ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6639-6646.2005
更新日期:2005-10-01 00:00:00
abstract::Oral inoculation of calves and steers with 10(10) CFU of Escherichia coli O157:H7 induced prompt and sustained increases in serum antibodies to O157 lipopolysaccharide. Neutralizing antibodies to verotoxin 1 also increased rapidly in most steers but more gradually in calves. None of the animals developed neutralizing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1879-1883.1996
更新日期:1996-05-01 00:00:00
abstract::Tuberculous pleurisy allows the study of human cells at the site of active Mycobacterium tuberculosis infection. In this study, we found that among pleural fluid (PF) lymphocytes, natural killer (NK) cells are a major source of early gamma interferon (IFN-gamma) upon M. tuberculosis stimulation, leading us to investig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00381-07
更新日期:2007-11-01 00:00:00
abstract::Modification of three or four of the five histidine residues in the toxic shock syndrome toxin 1 (TSST-1) with diethylpyrocarbonate did not inhibit the precipitin reaction of the modified TSST-1 with polyvalent antisera to the toxin. Monoclonal antibody 7T did not react with the modified TSST-1, but monoclonal antibod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.1901-1905.1989
更新日期:1989-07-01 00:00:00
abstract::We used an in vitro opsonophagocytic killing assay to measure the functional activity of antibody directed at the mucoid exopolysaccharide (MEP) antigen expressed by Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Rabbit antibodies raised to purified MEP were able to mediate phagocytic killing i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.2.281-285.1985
更新日期:1985-08-01 00:00:00
abstract::Toxicity of lipopolysaccharide (LPS) (endotoxin) is, to a large extent, mediated by the activation of monocytes/macrophages and subsequent release of monokines, such as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha). It is known that LPS binds readily to serum lipoproteins and that LPS-lipoprotein co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.2237-2245.1989
更新日期:1989-07-01 00:00:00
abstract::The pathogenicities of three proteases from Serratia marcescens, two proteases from Pseudomonas aeruginosa, and one thermolysin from Bacillus stearothermophilus were examined. All proteases tested caused acute liquefactive necrosis of the cornea and descemetocele formation in guinea pig eyes after intrastromal injecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2509-2517.1987
更新日期:1987-10-01 00:00:00
abstract::In Pseudomonas aeruginosa, the second messenger cyclic-di-GMP and Gac/Rsm signaling pathways are associated with the transition from acute to chronic infection. Therefore, identification of the molecular mechanisms that govern lifestyle choice in bacteria is very important. Here, we identified a novel cyclic-di-GMP mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00602-20
更新日期:2021-01-19 00:00:00
abstract::Brucella abortus is recognized by several Toll-like receptor (TLR)-associated pathways triggering proinflammatory responses that affect both the nature and intensity of the immune response. Previously, we demonstrated that B. abortus-mediated dendritic cell (DC) maturation and control of infection are dependent on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01356-12
更新日期:2013-05-01 00:00:00
abstract::Toxin preparations were obtained by growing Clostridium difficile VPI strain 10463 in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM-100 membrane filter. Two toxic activities, designated toxins A and B, were se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1032-1040.1982
更新日期:1982-03-01 00:00:00
abstract::Early-onset periodontitis (EOP) is characterized by rapidly progressive alveolar bone loss, chemotactic defects of neutrophils, and significant familial aggregation. We found immature myeloid lineage cells, defined as promyelocytes, in the peripheral blood in patients with EOP. A hematological examination of periphera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3906-3912.1997
更新日期:1997-09-01 00:00:00
abstract::Eight chlamydial isolates of ovine origin were tested in a plaque reduction system using homologous and heterologous rooster antisera. The eight isolates could be separated into two separate immunotypes. Type 1 included isolates associated with ovine abortion and one agent recovered from the feces of an apparently nor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.92-94.1974
更新日期:1974-01-01 00:00:00
abstract::The application of dissociation techniques, involving gel filtration in the presence of guanidine, to a semipurified preparation of Campylobacter jejuni heat-labile enterotoxin yielded a material whose functional and immunological properties resemble those of the B subunits of cholera toxin and Escherichia coli heat-l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.629-633.1985
更新日期:1985-06-01 00:00:00