Abstract:
:Increased protection against a virulent challenge with Mycobacterium tuberculosis is induced mainly by a previous immunization with living avirulent mycobacteria, usually Mycobacterium bovis BCG. Only a transient and marginal protection is obtained after immunization with bacterial extracts or dead bacteria. Both living and heat-killed bacteria share a number of common antigens. In order to identify mycobacterial molecules which are dominant antigens during immunization with living bacteria, a two-step selection method was used. Two groups of guinea pigs were immunized either with living or with heat-killed BCG. Sera were then collected and used to select and counterselect antigens present in BCG culture filtrates. Each major fraction eluted from a series of high-pressure liquid chromatography columns (gel filtration, DEAE, and reverse-phase chromatography) was run on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred on polyvinylidene difluoride sheets. The molecules present on twin immunoblots were stained with antibodies raised in guinea pigs immunized either with living or with heat-killed BCG. Cross-reactive antigens stained in twin immunoblots were eliminated. Major antigens interacting with antibodies raised after immunization only with living bacteria were further purified. A complex of 45- and 47-kDa major molecules (45/47-kDa complex) was thus identified and further purified. The complex was found to interact only with antibodies present in sera of guinea pigs immunized with living bacteria and not at all with antibodies raised after immunization with dead bacteria. The 45/47-kDa antigen complex molecules were resolved on two-dimensional electrophoresis in three major and seven minor proteins detected with silver staining. All the molecules interacted with the antibodies present in sera of guinea pigs immunized with living BCG. The three major proteins (two at 47 kDa and one at 45 kDa) were amino-terminal sequenced. The sequence A-P-E-P-A-P-P-V-P-P-A-A-A-A-P-P-A, which was not previously reported, was the same for these three molecules. By using a competitive enzyme-linked immunosorbent assay, the concentrations of the 45/47-kDa antigen complex were measured in BCG culture filtrates, freeze-dried BCG, and dried heat-killed BCG; they were, respectively, 2, 0.01, and 0.001% of the total mass. The low or very low values compared with the high antibody concentration emphasized the ability of the 45/47-kDa complex delivered through live BCG to trigger an antibody response.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Romain F,Laqueyrerie A,Militzer P,Pescher P,Chavarot P,Lagranderie M,Auregan G,Gheorghiu M,Marchal Gdoi
10.1128/IAI.61.2.742-750.1993subject
Has Abstractpub_date
1993-02-01 00:00:00pages
742-50issue
2eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Cryptosporidial infections were established in five young foals with severe combined immunodeficiency following oral administration of 10(8) Cryptosporidium parvum oocysts. All foals shed oocysts (average of 8 x 10(6) to 2 x 10(8)/g of feces) until death. Inflammation and C. parvum organisms were observed in the commo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3823-3826.1991
更新日期:1991-10-01 00:00:00
abstract::Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate effectors called Yersinia outer proteins (Yops) into infected host cells. Previous studies demonstrated a role for effector Yops in the inhibition of caspase-1-mediated cell death and secretion of interleukin-1beta (IL-1beta) in naïv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01695-07
更新日期:2008-09-01 00:00:00
abstract::A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into a bacteriophage lambda ZAP Express vector which contains both prokaryotic (P(lac)) and eukaryotic (P(CMV)) promoters upstream of the insertion site. The phage library is plated on Escher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.167-174.2006
更新日期:2006-01-01 00:00:00
abstract::Pertussis toxin is secreted from Bordetella pertussis with the assistance of the Ptl transport system, a member of the type IV family of macromolecular transporters. The S1 subunit and the B oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. To b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1193-1201.2002
更新日期:2002-03-01 00:00:00
abstract::The in vitro effects of pneumolysin, a sulfhydryl-activated toxin produced by Streptococcus pneumoniae, on various functions of human polymorphonuclear leukocytes (PMNLs) was investigated. Treatment of PMNLs with highly purified toxin significantly inhibited respiratory burst (in response to stimulation), ability to k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1212-1216.1983
更新日期:1983-09-01 00:00:00
abstract::Gram-negative bacterial pathogens utilize virulence-associated secretion systems to inject, or translocate, effector proteins into host cells to manipulate cellular processes and promote bacterial replication. However, translocated bacterial products are sensed by nucleotide binding domain and leucine-rich repeat-cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00778-16
更新日期:2017-09-20 00:00:00
abstract::Indomethacin, a nonsteroidal anti-inflammatory agent, will abolish salmonella-induced rabbit ileal secretion when given prior to the establishment of infection. In the present study, we assessed whether indomethacin can inhibit salmonella-induced intestinal secretion when administered after infection and net intestina...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.1.136-139.1977
更新日期:1977-07-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis is a major cause of food-borne diseases associated with consumption of shell eggs. Clinical isolates of S. enterica serovar Enteritidis exhibit a wide spectrum of virulence in mice. A highly virulent isolate (SE2472) was previously shown to be more resistant in vitro than other ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.451-461.2002
更新日期:2002-02-01 00:00:00
abstract::The properties of two candidate Salmonella typhi-based live oral typhoid vaccine strains, BRD691 (S. typhi Ty2 harboring mutations in aroA and aroC) and BRD1116 (S. typhi Ty2 harboring mutations in aroA, aroC, and htrA), were compared in a number of in vitro and in vivo assays. BRD1116 exhibited an increased susceptib...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.700-707.1999
更新日期:1999-02-01 00:00:00
abstract::Hepatitis, proliferative typhlitis, and colitis were characterized in young adult and older SCID/NCr mice naturally infected with Helicobacter hepaticus. Liver lesions consisted of Kupffer, Ito, and oval cell hyperplasia along with multifocal to coalescing coagulative hepatocyte necrosis. Numerous Warthin-Starry-posit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5477-5484.1998
更新日期:1998-11-01 00:00:00
abstract::The gene encoding Clostridium sordellii phospholipase C (Csp) was cloned and expressed as a histidine-tagged (His-tag) protein, and the protein was purified to compare its enzymatic and biological activities with those of Clostridium perfringens phospholipase C (Cpa) and Clostridium bifermentans phospholipase C (Cbp)....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.641-646.2003
更新日期:2003-02-01 00:00:00
abstract::In this study, cytokine patterns produced by CD4(+) T cells isolated from antrum or corpus gastral biopsy specimens of 10 patients with Helicobacter pylori-positive gastritis were compared. To this end, expression of intracellular cytokines (interleukin-4 [IL-4] and gamma interferon) and of CD4 was assessed by flow cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5543-5546.1998
更新日期:1998-11-01 00:00:00
abstract::This study examined apoptotic cell death associated with Shiga-like toxin (Stx)-producing Escherichia coli. Renal cortices from three children with postenteropathic hemolytic-uremic syndrome (HUS) and from mice infected with E. coli O157:H7 and pediatric renal tubular epithelial cells stimulated with Stx and E. coli O...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.636-644.1998
更新日期:1998-02-01 00:00:00
abstract::The purpose of our experiment was to evaluate the destructive potential of a strain of Actinomyces viscosus on the periodontium of sensitized rodents, describing the induced lesions on the basis of quantitative cytology. The experimental design comprised in principle the following procedures. Young germfree rats were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.3.565-577.1974
更新日期:1974-09-01 00:00:00
abstract::Group A streptococci of M protein type 5 have been epidemiologically related to acute rheumatic fever in a number of reported outbreaks. Preliminary bacteriological evidence suggests that M5 may be an important "rheumatogenic" type in Santiago, Chile. To assess further the relationship of this streptococcal serotype t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.573-579.1982
更新日期:1982-11-01 00:00:00
abstract::The application of dissociation techniques, involving gel filtration in the presence of guanidine, to a semipurified preparation of Campylobacter jejuni heat-labile enterotoxin yielded a material whose functional and immunological properties resemble those of the B subunits of cholera toxin and Escherichia coli heat-l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.629-633.1985
更新日期:1985-06-01 00:00:00
abstract::Infection with Cryptosporidium parvum, a ubiquitous protozoan parasite of virtually all mammals, can cause mild to severe diarrhea in immunocompetent hosts and life-threatening diarrhea in immunocompromised hosts. Passive immunotherapy of experimentally infected animals and naturally infected humans with hyperimmune b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2962-2965.1990
更新日期:1990-09-01 00:00:00
abstract::Bordetella bronchiseptica secreted a hydroxamate siderophore when grown in Fe-depleted medium. A Tn5lac insertion mutant of B. bronchiseptica, DBB22, did not produce this hydroxamate siderophore and was incapable of using lactoferrin as an Fe source. Our data suggest that B. bronchiseptica uses a siderophore for remov...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2698-2702.1993
更新日期:1993-06-01 00:00:00
abstract::Various factors affecting the aggregation of Actinomyces naeslundii strain 12104 were studied. When the pH of glucose-supplemented growth medium fell below 5.5, the cells aggregated and formed microbial masses which tenaciously adhered to the culture vessels. When the organism was cultured in the same medium in the ab...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.1003-1009.1978
更新日期:1978-09-01 00:00:00
abstract::Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1436-1441.2006
更新日期:2006-02-01 00:00:00
abstract::Onchocerca volvulus, a human parasitic nematode, is the third leading cause of preventable blindness worldwide. This study describes the molecular cloning of a novel superoxide dismutase (SOD) from the parasite. This putative O. volvulus extracellular SOD (OvEcSOD) is 628 nucleotides (nt) long, including a 22-nt 5' sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.713-716.1994
更新日期:1994-02-01 00:00:00
abstract::The effect of indomethacin on the net intestinal accumulation of fluid induced by Escherichia coli heat-stable (ST) enterotoxin in the infant mouse model was examined. Indomethacin, when administered with ST enterotoxin, caused a striking decrease in net intestinal fluid accumulation. This inhibition of ST activity wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.1.143-147.1978
更新日期:1978-10-01 00:00:00
abstract::The purpose of the present study was to analyze Chlamydia pneumoniae-induced, antigen-specific, cell-mediated immunity. Peripheral blood mononuclear cells of four persons infected with C. pneumoniae Kajaani 6 and 17 healthy volunteers were stimulated with antigen composed of whole elementary bodies of C. pneumoniae Ka...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2196-2199.1993
更新日期:1993-05-01 00:00:00
abstract::Pro- and anti-inflammatory cytokines and their signaling pathways play key roles in protection from and pathogenesis of mycobacterial infection, and their balance and dynamic changes may control or predict clinical outcome. Peripheral blood cells' capacity to produce proinflammatory (tumor necrosis factor alpha [TNF-a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00602-06
更新日期:2007-02-01 00:00:00
abstract::The M1inv+ subclone of M1 group A streptococci that spread globally in the late 1980s and early 1990s was previously identified by restriction fragment length polymorphism (RFLP), M protein, and SpeA exotoxin sequence analyses. Strains representing this subclone were characterized with regard to carriage of bacterioph...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5592-5597.1998
更新日期:1998-11-01 00:00:00
abstract::Bacillus cereus causes exotoxin-mediated diarrheal food poisoning. Hemolysin BL (HBL) is a well-characterized B. cereus toxin composed of three components (B, L1, and L2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. Here, we show that HBL causes fluid accumulation in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4423-4428.1995
更新日期:1995-11-01 00:00:00
abstract::Despite the autotransporter (AT) moniker, AT secretion appears to involve the function of periplasmic chaperones. We identified four periplasmic proteins that specifically bound to plasmid-encoded toxin (Pet), an AT produced by enteroaggregative Escherichia coli (EAEC). These proteins include the 17-kDa Skp chaperone ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00167-12
更新日期:2012-07-01 00:00:00
abstract::Yersinia pestis KIM5 was found to be cytotoxic for the IC21 and P388D1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from C57BL/6 mice. Affected cells phagocytosed KIM5 inefficiently, became spherical, detached readily from culture dishes, and retained 51Cr poorly. The cytotoxic effect wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.788-794.1986
更新日期:1986-03-01 00:00:00
abstract::Biofilm formation by the periodontal pathogen Aggregatibacter actinomycetemcomitans is dependent upon autoinducer-2 (AI-2)-mediated quorum sensing. However, the components that link the detection of the AI-2 signal to downstream gene expression have not been determined. One potential regulator is the QseBC two-compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01376-09
更新日期:2010-07-01 00:00:00
abstract::The initial encounter of Leishmania cells and cells from the immune system is fundamentally important in the outcome of infection and determines disease development or resistance. We evaluated the anti-Leishmania amazonensis response of naive volunteers by using an in vitro priming (IVP) system and comparing the respo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7453-7460.2001
更新日期:2001-12-01 00:00:00