Abstract:
:This study examined apoptotic cell death associated with Shiga-like toxin (Stx)-producing Escherichia coli. Renal cortices from three children with postenteropathic hemolytic-uremic syndrome (HUS) and from mice infected with E. coli O157:H7 and pediatric renal tubular epithelial cells stimulated with Stx and E. coli O157:H7 extracts were examined for apoptotic changes. Apoptotic cells were detected by terminal dUTP nick end labeling of tubuli and glomeruli from HUS patients and from mice inoculated with Stx-2-positive and Stx-negative strains. Apoptosis was more extensive and severe ultramorphological nuclear and cytoplasmic changes were seen in the Stx-2-positive group. Stx caused DNA fragmentation and ultramorphological changes indicating apoptosis in cultured pediatric tubular cells. DNA fragmentation increased when cells were pre-stimulated with tumor necrosis factor alpha. Polymyxin extracts from Stx-2-positive and Stx-negative strains induced DNA fragmentation, but only extracts from Stx-2-positive strains caused ultramorphological changes and extensive DNA fragmentation. The results indicate that HUS is accompanied by increased apoptosis of kidney cells and that bacterial factors, possibly together with host cytokines in vivo, may activate apoptotic tissue injury.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Karpman D,Håkansson A,Perez MT,Isaksson C,Carlemalm E,Caprioli A,Svanborg Cdoi
10.1128/IAI.66.2.636-644.1998subject
Has Abstractpub_date
1998-02-01 00:00:00pages
636-44issue
2eissn
0019-9567issn
1098-5522journal_volume
66pub_type
杂志文章abstract::The localization and population levels of the indigenous bacterial flora of conventional rats were investigated by cultural and histological techniques. Lactobacilli predominate in the stomach and upper part of the small intestine and associate with keratinized cells of the nonglandular portion of stomach. Mixtures of...
journal_title:Infection and immunity
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doi:10.1128/IAI.11.5.962-968.1975
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.59.12.4505-4510.1991
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.23.2.432-437.1979
更新日期:1979-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.565-570.1992
更新日期:1992-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.1997-1999.1987
更新日期:1987-09-01 00:00:00
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更新日期:2011-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1980-08-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.15.3.692-697.1977
更新日期:1977-03-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1980-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.599-608.1984
更新日期:1984-06-01 00:00:00
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pub_type: 杂志文章
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