Abstract:
:Mycoplasma gallisepticum is an avian respiratory and reproductive tract pathogen that has a significant economic impact on the poultry industry worldwide. Although membrane proteins of Mycoplasma spp. are thought to play crucial roles in host interactions, very few have had their biochemical function defined. In this study, we found that the GroEL protein (heat shock protein 60) of Mycoplasma gallisepticum could induce apoptosis in peripheral blood mononuclear cells, and the underlying molecular mechanism was further determined. The GroEL gene from Mycoplasma gallisepticum was cloned and expressed in Escherichia coli to facilitate the functional analysis of recombinant protein. The purified GroEL protein was shown to adhere to peripheral blood mononuclear cells (PBMCs) and DF-1 cells and cause apoptosis in PBMCs. A protein pulldown assay coupled with mass spectrometry identified that annexin A2 possibly interacted with GroEL protein. Coimmunoprecipitation assays confirmed that GroEL proteins could bind to annexin A2, and confocal analysis further demonstrated that GroEL colocolized with annexin A2 in HEK293T cells and PBMCs. Moreover, annexin A2 expression was significantly induced by a recombinant GroEL protein in PBMCs, and knocking down annexin A2 expression resulted in significantly reduced apoptosis. Taken together, these data suggest that GroEL induces apoptosis in host cells by interacting with annexin A2, a novel virulence mechanism in Mycoplasma gallisepticum Our findings lead to a better understanding of molecular pathogenesis in Mycoplasma gallisepticum.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Yu Y,Zhang L,Chen Y,Li Y,Wang Z,Li G,Wang G,Xin Jdoi
10.1128/IAI.00248-19subject
Has Abstractpub_date
2019-08-21 00:00:00issue
9eissn
0019-9567issn
1098-5522pii
IAI.00248-19journal_volume
87pub_type
杂志文章abstract::Listeria monocytogenes is an intracellular bacterial pathogen that invades epithelial cells by subverting two cellular receptors, E-cadherin and Met. We recently identified type II phosphatidylinositol 4-kinases alpha and beta (PI4KIIalpha and PI4KIIbeta) as being required for bacterial entry downstream of Met. In thi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00661-09
更新日期:2010-01-01 00:00:00
abstract::We examined the effect of protein kinase C (PKC) activation on the cyclic GMP response to heat-stable enterotoxin (STa) in a colonic carcinoma intestinal epithelial cell line, T84 cells. Our results demonstrate that the active phorbol ester analog, phorbol dibutyrate, but not the inactive alpha-phorbol dibutyrate, act...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1402-1407.1990
更新日期:1990-05-01 00:00:00
abstract::The infection of HeLa 229 cells in monolayer culture with trachoma (B/TW-5/OT) and lymphogranuloma venereum (LGV) (L2/434/Bu) organism was studied in terms of two parameters: radioactivity counts of cell-associated tritium labeled organisms at the initial stage of inoculation for measurement of attachment, and inclusi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1103-1109.1976
更新日期:1976-04-01 00:00:00
abstract::Acute lethality was induced in rabbits by the sequential injection of Propionibacterium acnes and lipopolysaccharide (LPS). P. acnes induced the infiltration of inflammatory cells into the liver lobules during the early phase, and LPS in the late phase caused death in association with pathological changes mimicking he...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4812-4817.1995
更新日期:1995-12-01 00:00:00
abstract::Hybridomas secreting monoclonal antibodies (MABs) specific for Clostridium perfringens type A enterotoxin were produced by fusion of P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with purified enterotoxin. Wells containing hybridomas secreting immunoglobulin G (IgG) antibodies against enterot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.442-448.1985
更新日期:1985-11-01 00:00:00
abstract::Smoking is associated with increased susceptibility to tuberculosis and influenza. However, little information is available on the mechanisms underlying this increased susceptibility. Mice were left unexposed or were exposed to cigarette smoke and then infected with Mycobacterium tuberculosis by aerosol or influenza A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00709-10
更新日期:2011-01-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::In the absence of antiserum, rabbit polymorphonuclear leukocytes (PMNs) released lysosomal enzymes in response to Actinomyces viscosus (19246) but not to Streptococcus mutans (6715). Antibodies had a marked modulating influence on these reactions. PMN hydrolase release was significantly enhanced to both organisms when...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.6.1315-1321.1976
更新日期:1976-12-01 00:00:00
abstract::In vivo- and in vitro-grown Mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium. Ciliostasis and loss of cilia in tracheal rings were induced by M. hyopneumoniae grown in vivo and with lo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5312-5318.1994
更新日期:1994-12-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) against herpes simplex virus (HSV) were induced in vitro from human peripheral blood lymphocytes by stimulation with HSV antigen. CTL generated by HSV type 1 (HSV-1) antigen stimulation killed not only HSV-1-infected target cells but also HSV type 2 (HSV-2)-infected target cells, though a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.190-197.1983
更新日期:1983-04-01 00:00:00
abstract::Of 202 captive hares studied, many of which had lesions on their external genital organs or testicular atrophy or both, 27% had positive serological tests for syphilis although dark-field examination of extracts of atrophic testes was negative. A total of 12 hares that were nonreactive for the serological test for syp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.231-234.1980
更新日期:1980-01-01 00:00:00
abstract::Chlamydia pneumoniae is a human respiratory pathogen. Unlike the other two Chlamydia species, no species-specific antigen has been defined for C. pneumoniae. An immunoreactive clone containing a 0.8-kb fragment was isolated from a C. pneumoniae (AR-39) genomic library by using anti-C. pneumoniae rabbit immune serum. B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.880-886.1994
更新日期:1994-03-01 00:00:00
abstract::Protozoan parasites of the genus Leishmania exist as obligatory intracellular amastigotes and invade macrophages and Langerhans cells, the dendritic cells of the skin. Langerhans cells are much more efficient in presenting Leishmania major antigen to T cells than macrophages are and have the unique ability to retain p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3444-3450.1997
更新日期:1997-08-01 00:00:00
abstract::Purification of the envelope antigen of Yersinia pestis EV with passive hemagglutination activity is described. The purification procedure consisted of pancreatin digestion, chromatography on human erythrocyte stroma set on Celite, and rechromatography on Sephadex G-200. Chemical, physical, and biological properties o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::Heterogeneic autoantibodies against axonal neurofilament proteins of mature mouse neurons grown in vitro were detected by the indirect immunofluorescence technique in 12.7% (9 of 71) of the sera from nonhuman primates infected with kuru, in 14.5% (17 of 117) and 4% (1 of 25), respectively, of the sera from nonhuman pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.316-324.1982
更新日期:1982-10-01 00:00:00
abstract::Rubella virus was capable of replicating in both unstimulated and phytohemagglutinin-stimulated cultures of human mononuclear blood cells. Monocyte-derived macrophages were the main cell type responsible for viral replication. The susceptibility of macrophages increased during cultivation. Phytohemagglutinin-stimulate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.309-314.1980
更新日期:1980-02-01 00:00:00
abstract::We demonstrate that transcription of the Staphylococcus aureus collagen adhesin gene (cna) is temporally regulated, with expression being highest in exponentially growing cultures and falling to almost undetectable levels as cultures enter the post-exponential-growth phase. The temporal regulation of cna transcription...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1536-1540.1997
更新日期:1997-04-01 00:00:00
abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00
abstract::Cytotoxic necrotizing factor type 1 (CNF1) and CNF2 are toxins of pathogenic Escherichia coli that share 85% identity over 1,014 amino acids. Although both of these toxins modify GTPases, CNF1 is a more potent inducer of multinucleation in HEp-2 cells, binds more efficiently to HEp-2 cells, and, despite the conservati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00943-08
更新日期:2009-01-01 00:00:00
abstract::Urinary tract infection (UTI) is the second most common infection in humans, making it a global health priority. Nearly half of all women will experience a symptomatic UTI, with uropathogenic Escherichia coli (UPEC) being the major causative agent of the infection. Although there has been extensive research on UPEC vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-17
更新日期:2018-02-20 00:00:00
abstract::Parasite antigens which bind to host cell molecules of approximately 32 and 34 kilodaltons (kDa) were identified in supernatant fluids obtained from axenic cultures of Trypanosoma cruzi. These parasite components were first detected in culture supernatants obtained after 2 weeks in culture. Immunoblot analysis of cult...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1812-1816.1990
更新日期:1990-06-01 00:00:00
abstract::The Salmonella dublin virulence plasmid mediates systemic infection in mice and cattle. Here, we analyze the interaction between wild-type and plasmid-cured Salmonella strains with phagocytes in vitro and in vivo. The intracellular recovery of S. dublin from murine peritoneal and bovine alveolar macrophages cultured i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3385-3393.1996
更新日期:1996-08-01 00:00:00
abstract::A nonproteolytic strain of Clostridium botulinum type B produces two toxins of different molecular weight (16S and 12S) that are indistinguishable from the corresponding toxins of a proteolytic strain in molecular weight and construction but differ in potential toxicity, activation ratio, and hemagglutinability. Succe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.987-989.1976
更新日期:1976-03-01 00:00:00
abstract::The purpose of our study was to characterize the properties of the interaction of pertussis toxin with human polymorphonuclear leukocytes for the modulation of leukotriene generation and metabolism. The cells were stimulated with either the Ca ionophore A23187, opsonized zymosan, or the bacterial peptide formyl-methio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3165-3171.1989
更新日期:1989-10-01 00:00:00
abstract::The ability of Klebsiella pneumoniae strains to resist the bactericidal activity of serum was quantitated. The K. pneumoniae strains tested included mutants lacking the capsular polysaccharide and mutants having a modified lipopolysaccharide structure. The last mutants were obtained as phage-resistant mutants, and the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.85-89.1986
更新日期:1986-10-01 00:00:00
abstract::Capsular types 5 and 8, which account for about 70% of Staphylococcus aureus strains isolated from the blood of patients, resisted in vitro phagocytosis by human polymorphonuclear leukocytes (PMN). Antisera and monoclonal antibody to type 5 and 8 capsular polysaccharides (CPS) induced type-specific in vitro phagocytos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1090-1095.1988
更新日期:1988-05-01 00:00:00
abstract::Mannan (MAN) extracted from Candida albicans 20A was investigated for its potential as an antigen in the detection of cell-mediated immunity (CMI) in vivo and in vitro and for its ability to modulate CMI when administered intravenously (i.v.). CBA/J mice were either immunized as adults by the cutaneous inoculation of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.693-700.1989
更新日期:1989-03-01 00:00:00
abstract::The invasion of red blood cells (RBCs) by Plasmodium falciparum is dependent on multiple molecular interactions between erythrocyte receptors and parasite ligands. Invasion studies using culture-adapted parasite strains have indicated significant receptor heterogeneity. It is not known whether this heterogeneity refle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5886-5891.2004
更新日期:2004-10-01 00:00:00
abstract::It has been reported that Neisseria gonorrhoeae possesses a very high level of catalase activity, but the regulation of catalase expression has not been investigated extensively. In Escherichia coli and Salmonella enterica serovar Typhimurium, it has been demonstrated that OxyR is a positive regulator of hydrogen pero...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.550-556.2003
更新日期:2003-01-01 00:00:00
abstract::The effect of a standard regimen of cimetidine on the gastric flora of 20 male volunteers was studied in a double-blind manner and compared with the effects of a standard antacid regimen. Postprandial microbial titers in gastric aspirates were significantly higher at 4, 8, and 16 weeks of therapy in subjects taking an...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.36.2.518-524.1982
更新日期:1982-05-01 00:00:00