Abstract:
:A nonproteolytic strain of Clostridium botulinum type B produces two toxins of different molecular weight (16S and 12S) that are indistinguishable from the corresponding toxins of a proteolytic strain in molecular weight and construction but differ in potential toxicity, activation ratio, and hemagglutinability. Successful hybridization between the toxic and nontoxic components (both7S) of 12S toxins of biologically heterologous type B strains confirmed the physico-chemical similarity between the toxic as well as the nontoxic components.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Miyazaki S,Kozaki S,Sakaguchi S,Sakaguchi Gdoi
10.1128/IAI.13.3.987-989.1976subject
Has Abstractpub_date
1976-03-01 00:00:00pages
987-9issue
3eissn
0019-9567issn
1098-5522journal_volume
13pub_type
杂志文章abstract::A 75-kilodalton major protein (75K protein) was purified to homogeneity from the cell lysate fraction and the envelope of Bacteroides gingivalis 381. The 75K protein was originally present in the outer membrane or the outermost part of this organism as a large, stable complex with an apparent molecular weight of about...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3646-3652.1989
更新日期:1989-11-01 00:00:00
abstract::We tested the hypothesis that the intensity of specific antichlamydial T cell-mediated immunity in the genital tract of female guinea pigs infected intravaginally with the chlamydial agent of guinea pig inclusion conjunctivitis would determine the resistance or susceptibility to reinfection after a primary chlamydial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1346-1351.1991
更新日期:1991-04-01 00:00:00
abstract::In the present study, the roles of interleukin 12 (IL-12) and IL-18 and their possible interaction during superantigen-induced responses were studied by injection of staphylococcal enterotoxin B (SEB) into mice. These data suggest that the role of IL-12 in SEB-induced responses is limited to sustaining gamma interfero...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5949-5952.2001
更新日期:2001-09-01 00:00:00
abstract::The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was identified, and the nucleotide sequence of the gene was determined. cofA consists of 714 nucleotides encoding a 238-amino-aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.724-728.1995
更新日期:1995-02-01 00:00:00
abstract::Tuberculosis is one of the most important infectious diseases worldwide. The susceptibility to this disease depends to a great extent on the innate immune response against mycobacteria. Host defense peptides (HDP) are one of the first barriers to counteract infection. Cathelicidin (LL-37) is an HDP that has many immun...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00936-15
更新日期:2015-12-01 00:00:00
abstract::Hemolytic-uremic syndrome (HUS) results from infection by Shiga toxin (Stx)-producing Escherichia coli and is the most common cause of acute renal failure in children. We have developed a mouse model of HUS by administering endotoxin-free Stx2 in multiple doses over 7 to 8 days. At sacrifice, moribund animals demonstr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00592-08
更新日期:2008-10-01 00:00:00
abstract::The interaction of plasma fibronectin with C1q of the complement system has been demonstrated in the past several years. In addition, the antibody-independent binding of C1q to bacteria, as well as the binding of plasma fibronectin to bacteria, is well documented. This study examines whether the binding of C1q to bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.664-669.1985
更新日期:1985-09-01 00:00:00
abstract::Humans infected with the dimorphic fungus Blastomyces dermatitidis develop strong T-lymphocyte responses to WI-1, an immunodominant antigen that has been shown to elicit protective immunity in mice. In the present study, the T-cell epitopes of WI-1 and human leukocyte antigen (HLA) restricting elements that display th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.502-510.2000
更新日期:2000-02-01 00:00:00
abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.1106-1108.1976
更新日期:1976-10-01 00:00:00
abstract::Strains of Bacteroides fragilis associated with diarrhea in children (termed enterotoxigenic B. fragilis, or ETBF) produce a heat-labile ca. 20-kDa protein toxin (BFT). The purpose of this study was to examine the activity of BFT on polarized monolayers of human intestinal epithelial cells (T84 cells). In Ussing chamb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3561-3570.1997
更新日期:1997-09-01 00:00:00
abstract::Although it has been known for some time that Salmonella typhimurium is able to survive and even replicate in the normally bactericidal environment of the macrophage phagosome, the mechanisms by which this organism accomplishes this feat remain obscure. In this study, a murine macrophage cell line and confocal immunof...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1475-1485.1997
更新日期:1997-04-01 00:00:00
abstract::Previously, in our laboratory, we established a two-chamber system to study translocation of Enterococcus faecalis across monolayers of polarized human colon carcinoma-derived T84 cells. By using the same system in the present study, we now show that disruption of gelE of strain OG1RF, which also has a polar effect on...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1606-1612.2005
更新日期:2005-03-01 00:00:00
abstract::We studied seven strains of group G streptococci isolated from clinically severe bacteremic infections in six intravenous drug abusers. These group G strains multiplied luxuriantly in fresh human blood. On electron microscopy, they exhibited surface fibrillae similar to those observed in M-protein-rich group A strepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.753-757.1987
更新日期:1987-03-01 00:00:00
abstract::Rabbit lymphoid cells from spleen, peripheral blood, and peritoneal cavity lacked killer (K)-cell activity against cell lines of rabbit and human origin, including virus-infected human tumor cells. This lack of activity was not affected by antibody concentration, source of antibodies, effector/target cell ratio, or le...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.489-496.1982
更新日期:1982-02-01 00:00:00
abstract::Ocular inflammation is one of the consequences of infection with the protozoan parasite Toxoplasma gondii. Even if lesions are self-healing in immunocompetent persons, they pose a lifetime risk of reactivation and are a serious threat to vision. As there are virtually no immunological data on reactivating ocular toxop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02985-14
更新日期:2015-05-01 00:00:00
abstract::Lipopolysaccharide (LPS) is cleared from the blood mainly by the liver. The Kupffer cells are primarily responsible for this clearance; liver endothelial and parenchymal cells contribute to a lesser extent. Although several binding sites have been described, only CD14 is known to be involved in LPS signalling. Among t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5107-5112.1998
更新日期:1998-11-01 00:00:00
abstract::A murine immunoglobulin G monoclonal antibody (MAb) raised against outer membrane vesicles of Moraxella catarrhalis 035E was shown to bind to a surface-exposed epitope of a major outer membrane protein of this organism. This outer membrane protein, which had an apparent molecular weight of approximately 80,000 in sodi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2003-2010.1993
更新日期:1993-05-01 00:00:00
abstract::Streptococcus pneumoniae is a major pathogen in humans. The pathogenicity of this organism is related to its many virulence factors, the most important of which is the thick pneumococcal capsule that minimizes phagocytosis. Another virulence-associated trait is the tendency of this bacterium to undergo autolysis in st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00290-09
更新日期:2009-09-01 00:00:00
abstract::To characterize the mechanism by which interleukin 10 (IL-10) inhibits Th1 responses to intracellular pathogens, we evaluated the interaction between IL-10 and Mycobacterium tuberculosis-induced gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells from persons across the spectrum of tuberculou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.913-918.1996
更新日期:1996-03-01 00:00:00
abstract::C-reactive protein (CRP) has long been known to appear in the sera of individuals with inflammatory processes, but its role in host defense against bacterial infection is unclear. We have recently demonstrated that CRP in the presence of the classical complement pathway markedly enhances the opsonization of Streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.987-992.1982
更新日期:1982-09-01 00:00:00
abstract::Mast cell-deficient W/Wv mice and their mast cell-sufficient littermates were given infections of Trichinella spiralis. W/Wv mice were slower than their littermates to expel adult T. spiralis. Repair of the mast cell deficiency of W/Wv mice by bone marrow grafting was accompanied by accelerated expulsion of T. spirali...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.445-447.1983
更新日期:1983-07-01 00:00:00
abstract::We utilized magnetic resonance imaging to visualize lesions in the lungs of guinea pigs infected by low-dose aerosol exposure to Mycobacterium tuberculosis. Lesions were prominent in such images, and colorized three-dimensional reconstructions of images revealed a very uniform distribution in the lungs. Lesion numbers...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5963-5971.2004
更新日期:2004-10-01 00:00:00
abstract::Fimbriae, lipopolysaccharide (LPS), and extracellular polymeric substance (EPS) all contribute to biofilm formation by the periodontopathogen Aggregatibacter actinomycetemcomitans. To understand how individual biofilm determinants respond to changing environmental conditions, the transcription of genes responsible for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00126-09
更新日期:2009-07-01 00:00:00
abstract::Three antigenic determinants were revealed in H3 hemagglutinin of influenza A viruses isolated from 1968 to 1975. One of them was common for all viruses, and two others specified differences between the viruses possessing H3 hemagglutinin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.804-807.1979
更新日期:1979-06-01 00:00:00
abstract::Toxigenic Vibrio cholerae strains are lysogens of CTXPhi, a filamentous phage which encodes cholera toxin. The receptor for CTXPhi for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 146 CTX-negative stra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5819-5825.1998
更新日期:1998-12-01 00:00:00
abstract::A set of four monoclonal antibodies was produced against a highly purified pepsin extract of type 5 streptococcal M protein. Three of the four antibodies cross-reacted with purified M proteins from heterologous serotypes and opsonized the respective heterologous organisms. Our studies suggest that monoclonal antibodie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.267-269.1984
更新日期:1984-10-01 00:00:00
abstract::Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the immunogenicity of three surface-exposed proteins from serogroup B Neisseria meningitidis (App, NhhA, and NadA) identified du...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4052-4060.2004
更新日期:2004-07-01 00:00:00
abstract::A monoclonal antibody was used to characterize a 135-kDa surface-located membrane protein (Lmp1) generally present in Mycoplasma hominis strains. The monoclonal antibody, 552, was applied to identify the corresponding gene in an expression library of M. hominis PG21 DNA. The M. hominis PG21 lmp1 gene was sequenced, an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.212-223.1995
更新日期:1995-01-01 00:00:00
abstract::Lipopolysaccharides obtained from Brucella abortus, B. melitensis, and B. suis, but not B. canis, were found to contain amino sugars identified as glucosamine and quinovosamine by cation exchange and thin-layer cellulose chromatography and ninhydrin degradation. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.4.772-774.1974
更新日期:1974-04-01 00:00:00
abstract::Three-month-old male Crl:CD1(ICR)BR and C3H/HeJ mice were fed chow containing Candida albicans for 14 days, while similar control mice were fed regular food. Stool cultures were done for all mice before and after administration of the special diet. Stool cultures were repeated 48 h, 1 week, and 1 and 3 months after st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1514-1517.1990
更新日期:1990-06-01 00:00:00