Invasion profiles of Brazilian field isolates of Plasmodium falciparum: phenotypic and genotypic analyses.


:The invasion of red blood cells (RBCs) by Plasmodium falciparum is dependent on multiple molecular interactions between erythrocyte receptors and parasite ligands. Invasion studies using culture-adapted parasite strains have indicated significant receptor heterogeneity. It is not known whether this heterogeneity reflects the parasite invasion arsenal in the field. We have studied the invasion phenotypes of 14 distinct field isolates from the Legal Amazon areas of Brazil by using erythrocyte invasion assays to investigate invasion into normal, enzyme-treated, and clinical-mutant RBCs. Analysis of these isolates revealed four distinct invasion profiles. Using En(a-) cells to get an unequivocal estimate of the use of glycophorin A (GPA) as a receptor, we found that the 175-kDa erythrocyte-binding antigen (EBA-175)/GPA pathway was used by a minority of the parasite isolates studied. Although polymorphism of region II domains at specific amino acid positions in both EBA-140 and EBA-181 was found in these field isolates, this did not correlate with invasion profiles and thus receptor selectivity. These studies have further confirmed the existence of a significant diversity of invasion pathways in nature and suggest that additional parasite ligands will have to be targeted to devise global vaccines that will work in the field.


Infect Immun


Infection and immunity


Lobo CA,de Frazao K,Rodriguez M,Reid M,Zalis M,Lustigman S





Has Abstract


2004-10-01 00:00:00














  • Activity and specificity of a mouse monoclonal antibody to ferric aerobactin.

    abstract::We isolated a monoclonal antibody directed against the ferric complex of aerobactin purified from Escherichia coli KH576. This antibody, which we designated MAb AERO1, was identified as an immunoglobulin G, subtype 2. A competitive enzyme-linked immunosorbent assay with MAb AERO1 had a limit of 10 nM for the detection...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: le Roy D,Expert D,Razafindratsita A,Deroussent A,Cosme J,Bohuon C,Andremont A

    更新日期:1992-03-01 00:00:00

  • Role of cytokines and major histocompatibility complex restriction in mouse resistance to infection with a natural recombinant strain (type I-III) of Toxoplasma gondii.

    abstract::Herein we characterized various genetic markers and the biological behavior of a natural recombinant strain of Toxoplasma gondii (P-Br). From nine genetic markers analyzed, three (B1, ROP1, and SAG1) and three (cS10-A6, GRA6, and SAG3) markers belong to parasites from the type I and type III lineages, respectively. Th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fux B,Rodrigues CV,Portela RW,Silva NM,Su C,Sibley D,Vitor RW,Gazzinelli RT

    更新日期:2003-11-01 00:00:00

  • Saccharomyces boulardii stimulates intestinal immunoglobulin A immune response to Clostridium difficile toxin A in mice.

    abstract::Saccharomyces boulardii is a nonpathogenic yeast that protects against antibiotic-associated diarrhea and recurrent Clostridium difficile colitis. The administration of C. difficile toxoid A by gavage to S. boulardii-fed BALB/c mice caused a 1.8-fold increase in total small intestinal immunoglobulin A levels (P = 0.00...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Qamar A,Aboudola S,Warny M,Michetti P,Pothoulakis C,LaMont JT,Kelly CP

    更新日期:2001-04-01 00:00:00

  • Phagocytosis of microbial L-forms.

    abstract::Little data are available regarding L-form phagocyte interactions. Due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. Intracellular localization of L-f...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Harwick HJ,Barajas L,Montgomerie JZ,Kalmanson GM,Guze LB

    更新日期:1972-06-01 00:00:00

  • Role of the surface lipoprotein BBA07 in the enzootic cycle of Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the Lyme disease pathogen, dramatically alters its protein profile when it is transmitted between ticks and mammals. Several differentially expressed proteins have been shown to be critical for the enzootic cycle of B. burgdorferi. In this study, we demonstrated that expression of the surface lip...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Xu H,He M,He JJ,Yang XF

    更新日期:2010-07-01 00:00:00

  • Respiratory infection with attenuated Mycobacterium tuberculosis H37Ra in malnourished guinea pigs.

    abstract::Specific pathogen-free guinea pigs were infected via the respiratory route with viable, attenuated Mycobacterium tuberculosis H37Ra and maintained on purified isocaloric diets. The control diet contained 30% protein (ovalbumin) and 50 ppm of added zinc (50 micrograms/g), the low protein diet contained 10% protein and ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McMurray DN,Carlomagno MA,Cumberland PA

    更新日期:1983-02-01 00:00:00

  • Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis.

    abstract::To assess the humoral immunological responses at the subclass level in shigellosis, specific antibody responses against Shigella dysenteriae 1 lipopolysaccharide (LPS), S. flexneri Y LPS, invasion plasmid-coded protein antigens (Ipa), and Shiga toxin were analyzed. Antibody responses of 41 patients with S. dysenteriae...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Islam D,Wretlind B,Ryd M,Lindberg AA,Christensson B

    更新日期:1995-05-01 00:00:00

  • Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens.

    abstract::Three strains of Lactobacillus, identified as Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus jensenii, were selected from among 70 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization or antagonism. All three self-aggregated and adhered to epithe...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Boris S,Suárez JE,Vázquez F,Barbés C

    更新日期:1998-05-01 00:00:00

  • Essential role for estrogen in protection against Vibrio vulnificus-induced endotoxic shock.

    abstract::Little is known about the underlying mechanisms that result in a sexually dimorphic response to Vibrio vulnificus endotoxic shock. V. vulnificus is a gram-negative bacterium, considered one of the most invasive and rapidly fatal human pathogens known. However, 85% of individuals that develop endotoxic shock from V. vu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Merkel SM,Alexander S,Zufall E,Oliver JD,Huet-Hudson YM

    更新日期:2001-10-01 00:00:00

  • Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates.

    abstract::Detailed structural studies were undertaken on five isolates of bovine rotavirus which showed variability in the migration patterns of their genome segments on electrophoresis in polyacrylamide gels. The individual genome segments of each isolate were characterized by partial digestion of terminally radiolabeled RNA w...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Clarke IN,McCrae MA

    更新日期:1982-05-01 00:00:00

  • Localization of high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae by immunoelectron microscopy.

    abstract::A family of high-molecular-weight (HMW) surface-exposed proteins important in the attachment of nontypeable Haemophilus influenzae (NTHi) to human epithelial cells was previously identified (J. W. St. Geme III, S. Falkow, and S. J. Barenkamp, Proc. Natl. Acad. Sci. USA 90:2875-2879, 1993). In the present investigation...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bakaletz LO,Barenkamp SJ

    更新日期:1994-10-01 00:00:00

  • Unexpected Role of CD8 T Cells in Accelerated Clearance of Salmonella enterica Serovar Typhimurium from H-2 Congenic mice.

    abstract::Salmonella infection can cause gastroenteritis in healthy individuals or a serious, systemic infection in immunocompromised patients and has a global impact. CD4 Th1 cells represent the main lymphocyte population that participates in bacterial clearance during both primary and secondary infections in mice of the H-2b ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Labuda JC,Depew CE,Pham OH,Benoun JM,Ramirez NA,McSorley SJ

    更新日期:2019-10-18 00:00:00

  • Role of the bacterial cell wall in middle ear inflammation caused by Streptococcus pneumoniae.

    abstract::The pathogenesis of middle ear inflammation caused by Streptococcus pneumoniae was explored in the chinchilla model with different pneumococcal cell wall (CW) preparations, including isolated native CW, M1 muramidase CW (M1-CW) digest, amidase CW digest, and M1 peptidoglycan (M1-PG) digest. Inflammatory cell and lysoz...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Carlsen BD,Kawana M,Kawana C,Tomasz A,Giebink GS

    更新日期:1992-07-01 00:00:00

  • Demonstration of a new type of immunoglobulin G receptor in Streptococcus zooepidemicus strains.

    abstract::Forty-seven bacterial strains representing four different group C streptococcal species were tested for binding of human and bovine immunoglobulin G (IgG) subclasses. Specific binding sites for IgG were found in all bacterial species studied. The four species included differed, however, in their capacities to interact...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Myhre EB,Kronvall G

    更新日期:1980-03-01 00:00:00

  • Contribution of each of four Superantigens to Streptococcus equi-induced mitogenicity, gamma interferon synthesis, and immunity.

    abstract::Streptococcus equi is the causative agent of strangles, the most frequently diagnosed infectious disease of horses worldwide. The disease is characterized by abscessation and swelling of the lymph nodes of the head and neck, which can literally strangle the horse to death. S. equi produces four recently acquired phage...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Paillot R,Robinson C,Steward K,Wright N,Jourdan T,Butcher N,Heather Z,Waller AS

    更新日期:2010-04-01 00:00:00

  • Spectrum of Trained Innate Immunity Induced by Low-Virulence Candida Species against Lethal Polymicrobial Intra-abdominal Infection.

    abstract::Polymicrobial intra-abdominal infections (IAI) are clinically prevalent and cause significant morbidity and mortality, especially those involving fungi. Our laboratory developed a mouse model of polymicrobial IAI and demonstrated that coinfection with Candida albicans and Staphylococcus aureus (C. albicans/S. aureus) ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lilly EA,Yano J,Esher SK,Hardie E,Fidel PL Jr,Noverr MC

    更新日期:2019-07-23 00:00:00

  • Detection of established virulence genes and plasmids to differentiate Borrelia burgdorferi strains.

    abstract::Borrelia burgdorferi sensu stricto is the major causative agent of Lyme disease in the United States, while B. garinii and B. afzelii are more prevalent in Europe. The highly complex genome of B. burgdorferi is comprised of a linear chromosome and a large number of variably sized linear and circular plasmids. Many pla...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chan K,Casjens S,Parveen N

    更新日期:2012-04-01 00:00:00

  • Immunologic and structural relationships of the minor pilus subunits among Haemophilus influenzae isolates.

    abstract::Two proteins, HifD and HifE, have been identified as structural components of Haemophilus influenzae pili. Both are localized at the pilus tip, and HifE appears to mediate pilus adherence to host cells. In this study we examined the immunologic and structural diversity of these pilus subunits among type b H. influenza...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McCrea KW,Sauver JL,Marrs CF,Clemans D,Gilsdorf JR

    更新日期:1998-10-01 00:00:00

  • Survival of Salmonella enterica serovar Typhimurium within late endosomal-lysosomal compartments of B lymphocytes is associated with the inability to use the vacuolar alternative major histocompatibility complex class I antigen-processing pathway.

    abstract::Gamma interferon (IFN-gamma)-activated macrophages use an alternative processing mechanism to present Salmonella antigens to CD8(+) T lymphocytes. This pathway involves processing of antigen in a vacuolar compartment followed by secretion and loading of antigenic peptides to major histocompatibility complex class I (M...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rosales-Reyes R,Alpuche-Aranda C,Ramírez-Aguilar Mde L,Castro-Eguiluz AD,Ortiz-Navarrete V

    更新日期:2005-07-01 00:00:00

  • Further analysis of the sequence of the S1 subunit of pertussis toxin.

    abstract::The two published sequences of the pertussis toxin operon differ in 3 bp in the S1 subunit gene. In this report, we provide evidence that Bordetella pertussis strains are able to produce active pertussis toxin only when they contain one of the two possible nucleotide sequences. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pizza M,Bugnoli M,Pucci P,Siciliano R,Marino G,Rappuoli R

    更新日期:1991-03-01 00:00:00

  • Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrheas.

    abstract::We used the Salmonella typhi galactose epimerase (galE) mutant strain Ty21a, shown to be a safe, effective, living, attenuated oral typhoid vaccine, as a recipient for a recombinant plasmid containing the gene for production of the nontoxic B subunit of the heat-labile enterotoxin of Escherichia coli. The S. typhi der...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Clements JD,El-Morshidy S

    更新日期:1984-11-01 00:00:00

  • Exposure to low oxygen tension and increased osmolarity enhance the ability of Mycobacterium avium to enter intestinal epithelial (HT-29) cells.

    abstract::Current evidence indicates that Mycobacterium avium infection in patients with AIDS is acquired mostly through the gastrointestinal (GI) tract and that M. avium binds to and invades GI mucosal cells in vitro. Since M. avium is exposed to specific environmental conditions in the GI tract such as changes in pH, low oxyg...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bermudez LE,Petrofsky M,Goodman J

    更新日期:1997-09-01 00:00:00

  • A model of sustained gastrointestinal colonization by Candida albicans in healthy adult mice.

    abstract::Three-month-old male Crl:CD1(ICR)BR and C3H/HeJ mice were fed chow containing Candida albicans for 14 days, while similar control mice were fed regular food. Stool cultures were done for all mice before and after administration of the special diet. Stool cultures were repeated 48 h, 1 week, and 1 and 3 months after st...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Samonis G,Anaissie EJ,Rosenbaum B,Bodey GP

    更新日期:1990-06-01 00:00:00

  • Sensing of interleukin-1 cytokines during Streptococcus pneumoniae colonization contributes to macrophage recruitment and bacterial clearance.

    abstract::Streptococcus pneumoniae (the pneumococcus), a leading cause of bacterial disease, is most commonly carried in the human nasopharynx. Colonization induces inflammation that promotes the organism's growth and transmission. This inflammatory response is dependent on intracellular sensing of bacterial components that acc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lemon JK,Miller MR,Weiser JN

    更新日期:2015-08-01 00:00:00

  • Staphylococcus aureus Colonization Induces Strain-Specific Suppression of Interleukin-17.

    abstract::Staphylococcus aureus is a pathogen that causes significant morbidity and mortality. Nasal carriage is a major source of transmission and of endogenous infection. Persistent carriage is detected in ∼30% of healthy individuals. While Th17 cells have been shown to play a role in S. aureus infection and clearance, the im...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Reiss-Mandel A,Rubin C,Zayoud M,Rahav G,Regev-Yochay G

    更新日期:2018-02-20 00:00:00

  • A peptide permease mutant of Mycobacterium bovis BCG resistant to the toxic peptides glutathione and S-nitrosoglutathione.

    abstract::Oligopeptides play important roles in bacterial nutrition and signaling. Using sequences from the available genome database for Mycobacterium tuberculosis H37Rv, the oligopeptide permease operon (oppBCDA) of Mycobacterium bovis BCG was cloned from a cosmid library. An opp mutant strain was constructed by homologous re...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Green RM,Seth A,Connell ND

    更新日期:2000-02-01 00:00:00

  • Involvement of sortase anchoring of cell wall proteins in biofilm formation by Streptococcus mutans.

    abstract::Streptococcus mutans is one of the best-known biofilm-forming organisms associated with humans. We investigated the role of the sortase gene (srtA) in monospecies biofilm formation and observed that inactivation of srtA caused a decrease in biofilm formation. Genes encoding three putative sortase-dependent proteins we...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lévesque CM,Voronejskaia E,Huang YC,Mair RW,Ellen RP,Cvitkovitch DG

    更新日期:2005-06-01 00:00:00

  • Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.

    abstract::Escherichia coli is the most common cause of urinary tract infection. Elevated blood and urine interleukin-6 (IL-6) levels have been shown in inflammatory urinary tract diseases. The role of IL-6 in mediating the urodynamic dysfunction in response to E. coli-induced urinary tract infection has not yet been fully eluci...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weng TI,Wu HY,Lin PY,Liu SH

    更新日期:2009-08-01 00:00:00

  • Experimental model for dermal granulomatous hypersensitivity in Q fever.

    abstract::Q fever has been associated with granulomatous changes in clinical biopsy material obtained from liver and bone marrow. Local reactions to skin testing have been described in previously sensitized humans, but histological studies of such reactions have not been reported. We note that delayed hypersensitivity reactions...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ascher MS,Berman MA,Parker D,Turk JL

    更新日期:1983-01-01 00:00:00

  • Platelet receptors for the Streptococcus sanguis adhesin and aggregation-associated antigens are distinguished by anti-idiotypical monoclonal antibodies.

    abstract::Platelets aggregate in response to an adhesin and the platelet aggregation-associated protein (PAAP) expressed on the cell surfaces of certain strains of Streptococcus sanguis. We sought to identify the corresponding PAAP receptor and accessory adhesin binding sites on platelets. Since the adhesion(s) of S. sanguis fo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gong K,Wen DY,Ouyang T,Rao AT,Herzberg MC

    更新日期:1995-09-01 00:00:00