Abstract:
:Listeria monocytogenes is an intracellular bacterial pathogen that invades epithelial cells by subverting two cellular receptors, E-cadherin and Met. We recently identified type II phosphatidylinositol 4-kinases alpha and beta (PI4KIIalpha and PI4KIIbeta) as being required for bacterial entry downstream of Met. In this work, we investigated whether tetraspanins CD9, CD63, and CD81, which figure among the few described molecular partners of PI4KIIalpha, function as molecular adaptors recruiting PI4KIIalpha to the bacterial entry site. We observed by fluorescence microscopy that CD9, CD63, and CD81 are expressed and detected at the cellular surface and also within intracellular compartments, particularly in the case of CD63. In resting cells, colocalization of tetraspanins and PI4KIIalpha is detectable only in restricted areas of the perinuclear region. Upon infection with Listeria, endogenous CD9, CD63, and CD81 were recruited to the bacterial entry site but did not colocalize strictly with endogenous PI4KIIalpha. Live-cell imaging confirmed that tetraspanins and PI4KIIalpha do not follow the same recruitment dynamics to the Listeria entry site. Depletion of CD9, CD63, and CD81 levels by small interfering RNA demonstrated that CD81 is required for bacterial internalization, identifying for the first time a role for a member of the tetraspanin family in the entry of Listeria into target cells. Moreover, depletion of CD81 inhibits the recruitment of PI4KIIalpha but not that of the Met receptor to the bacterial entry site, suggesting that CD81 may act as a membrane organizer required for the integrity of signaling events occurring at Listeria entry sites.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tham TN,Gouin E,Rubinstein E,Boucheix C,Cossart P,Pizarro-Cerda Jdoi
10.1128/IAI.00661-09subject
Has Abstractpub_date
2010-01-01 00:00:00pages
204-9issue
1eissn
0019-9567issn
1098-5522pii
IAI.00661-09journal_volume
78pub_type
杂志文章abstract::Cell-free hemoglobin (Hb) is being developed as an erythrocyte substitute. We have previously demonstrated that cell-free Hb is an endotoxin-binding protein which disaggregates endotoxin and subsequently increases the biological activity of endotoxin in several in vitro assays. Because much of the morbidity and mortal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1258-1266.1997
更新日期:1997-04-01 00:00:00
abstract::Organ cultures of adult hamster trachea were used to evaluate the cytotoxic potential of cell fractions of Mycoplasma pneumoniae. Cytoplasm was essentially devoid of activity, whereas viable cells and membrane preparations, at a level of 25 mug of protein per ml, induced necrosis. Damage, as revealed by light and elec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1127-1134.1974
更新日期:1974-11-01 00:00:00
abstract::Upon infection of a host, the pathogenic fungus Aspergillus fumigatus is attacked by the reactive oxygen species produced by phagocytic cells. Detoxification of hydrogen peroxide by catalases was proposed as a way to overcome this host response. A. fumigatus produces three active catalases; one is produced by conidia,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3551-3562.2003
更新日期:2003-06-01 00:00:00
abstract::Hybridomas secreting monoclonal antibodies (MABs) specific for Clostridium perfringens type A enterotoxin were produced by fusion of P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with purified enterotoxin. Wells containing hybridomas secreting immunoglobulin G (IgG) antibodies against enterot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.442-448.1985
更新日期:1985-11-01 00:00:00
abstract::Burkholderia pseudomallei, a causative agent of melioidosis, is a Gram-negative facultative intracellular bacterium that can survive and multiply in macrophages. Previously, we demonstrated that B. pseudomallei failed to activate gene expression downstream of the MyD88-independent pathway, particularly the expression ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01254-10
更新日期:2011-07-01 00:00:00
abstract::Previous studies have demonstrated antigenic differences among the pili expressed by various strains of Haemophilus influenzae type b (Hib). In order to understand the molecular basis for these differences, the structural gene for pilin was cloned from Hib strain Eagan (p+) and the nucleotide sequence was compared to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1991-1996.1991
更新日期:1991-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.278-280.1980
更新日期:1980-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3428-3432.1992
更新日期:1992-08-01 00:00:00
abstract::Rabbit endogenous pyrogens occurred in two forms. One was an apparently single protein with a pI of 7.3; the other was a family of proteins with pI values of 4.5 to 5.0. We selected two of the latter, with pI values of 4.6 and 4.72, as representative of the group and compared them with the pI 7.3 pyrogen. Antisera rai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.177-183.1981
更新日期:1981-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.647-653.1982
更新日期:1982-02-01 00:00:00
abstract::Transforming growth factor beta (TGF-beta) has been shown to be a central immunomodulator used by leishmaniae to escape effective mechanisms of protection in human and murine infections with these parasites. However, all the information is derived from studies of established infection, while little is known about TGF-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5908-5914.2005
更新日期:2005-09-01 00:00:00
abstract::Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3787-3791.2000
更新日期:2000-06-01 00:00:00
abstract::Cyclic dimeric GMP (c-di-GMP), a bacterial second messenger, is known to regulate bacterial biofilm and sessility. Replication of an obligatory intracellular pathogen, Ehrlichia chaffeensis, is characterized by formation of bacterial aggregates called morulae inside membrane-bound inclusions. When E. chaffeensis matur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05320-11
更新日期:2011-10-01 00:00:00
abstract::Serum from rabbits immunized with either heat-killed or live nonencapsulated Staphylococcus aureus failed further to enhance phagocytosis and intracellular killing of the homologous organism by either normal rabbit polymorphonuclear leukocytes or monocytes, when compared with normal rabbit serum. These immune sera did...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.6.742-749.1970
更新日期:1970-12-01 00:00:00
abstract::Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies, but was more pronou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.84-90.1977
更新日期:1977-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.768-772.1992
更新日期:1992-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05209-11
更新日期:2011-11-01 00:00:00
abstract::Streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. Since one hypothesis of the pathogenesis of S. suis infection is that bacteria enter the bloodstream and invade the meninges and other tissues in close association with mononuclear phagocytes, the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4312-4322.2002
更新日期:2002-08-01 00:00:00
abstract::BCG vaccines are a family of closely related daughter strains of an attenuated isolate of Mycobacterium bovis derived by in vitro passage from 1908 to 1921. During subsequent laboratory propagation of the vaccine strain until its lyophilization in 1961, BCG Pasteur underwent at least seven further genomic mutations. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2803-2809.2004
更新日期:2004-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1857-1864.2006
更新日期:2006-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4135-4144.2000
更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.737-740.1984
更新日期:1984-09-01 00:00:00
abstract::We examined the immunobiological responses to Histoplasma capsulatum in lungs of gamma interferon (IFN-gamma) knockout mice (GKO mice). Naive GKO mice succumbed by day 9 to intranasal challenge with 2.5 x 10(6) yeasts, whereas all wild-type (WT) mice survived for 45 days. Compared to lungs of WT mice, the lungs of acu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2564-2569.1997
更新日期:1997-07-01 00:00:00
abstract::The effect of a standard regimen of cimetidine on the gastric flora of 20 male volunteers was studied in a double-blind manner and compared with the effects of a standard antacid regimen. Postprandial microbial titers in gastric aspirates were significantly higher at 4, 8, and 16 weeks of therapy in subjects taking an...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.36.2.518-524.1982
更新日期:1982-05-01 00:00:00
abstract::The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1, a gene of C. albicans that causes Saccharomyces cerevisiae to flocculate and adhere to endothelial cells. We then characterized multiple heterozygous and homozygous mutants. These nu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3193-3198.1999
更新日期:1999-07-01 00:00:00
abstract::The hagfish, Eptatretus stoutii, has been shown to synthesize bactericidal antibodies after injections of gram-negative organisms. These bactericidins could be detected as early as 2 days after the primary injection. Secondary responses were accelerated, although titers were not significantly higher than those reached...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.2.160-166.1971
更新日期:1971-08-01 00:00:00
abstract::The 260-kDa galactose-inhibitable lectin of Entamoeba histolytica mediates binding of amebic trophozoites to purified colonic mucins and intestinal epithelial cells. Parenteral immunization of Lewis rats with immuno-affinity-purified lectin with Freund's adjuvant and then intra-Peyer's patch inoculation of lectin with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.686-689.1995
更新日期:1995-02-01 00:00:00
abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::Intrauterine infections lead to serious complications for mother and fetus, including preterm birth, maternal and fetal death, and neurological sequelae in the surviving offspring. Improving maternal and child heath is a global priority. Yet, the development of strategies to prevent and treat pregnancy-related disease...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00801-17
更新日期:2018-05-22 00:00:00
abstract::An inhibitory factor from Actinobacillus actinomycetemcomitans Y4 was isolated, and its properties indicated that it was a bacteriocin (actinobacillicin). The bacteriocin was active against Streptococcus sanguis strains, Streptococcus uberis (FDC1), and Actinomyces viscosus T14 as well as other strains of A. actinomyc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.686-691.1987
更新日期:1987-03-01 00:00:00