Unanticipated heterogeneity in growth rate and virulence among Candida albicans AAF1 null mutants.

Abstract:

:The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1, a gene of C. albicans that causes Saccharomyces cerevisiae to flocculate and adhere to endothelial cells. We then characterized multiple heterozygous and homozygous mutants. These null mutants adhered to endothelial cells to the same extent as did the parent organism. However, mutants with presumably the same genotype revealed significant heterogeneity in their growth rates in vitro. This heterogeneity was not the result of the transformation procedure per se, nor was it caused by differences in the expression or function of URA3, a marker used in the process of gene disruption. The growth rate among the different heterozygous and homozygous null mutants was positively correlated with in vivo virulence in mice. It is possible that the variable phenotypes of C. albicans were due to mutations outside of the AAF1 coding region that were introduced during the gene disruption process. These results indicate that careful phenotypic characterization of mutants of C. albicans generated through targeted gene disruption should be performed to exclude the introduction of unexpected mutations that may influence pathogenicity in mice.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Rieg G,Fu Y,Ibrahim AS,Zhou X,Filler SG,Edwards JE Jr

doi

10.1128/IAI.67.7.3193-3198.1999

keywords:

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

3193-8

issue

7

eissn

0019-9567

issn

1098-5522

journal_volume

67

pub_type

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