Abstract:
:The in vitro effects of Pseudomonas aeruginosa elastase (P. aeruginosa E) and of human leukocyte elastase on human airway lysozyme (HAL) were investigated. P. aeruginosa E inactivated and cleaved HAL, whereas human leukocyte elastase had no effect. Total inactivation of HAL by P. aeruginosa E was observed after 120 min of incubation at 37 degrees C, for an elastase-to-lysozyme molar ratio of 1:5. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of reaction mixtures containing HAL and P. aeruginosa E in an elastase-to-lysozyme molar ratio of 1:10 showed a progressive disappearance of the HAL band upon increasing the incubation time with P. aeruginosa E. Gel filtration chromatography indicated that HAL was cleaved into at least three peptide fragments. The cleavage of HAL by P. aeruginosa E was accompanied by parallel losses of its bacteriolytic activity and its immunoreactive property.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Jacquot J,Tournier JM,Puchelle Edoi
10.1128/IAI.47.2.555-560.1985subject
Has Abstractpub_date
1985-02-01 00:00:00pages
555-60issue
2eissn
0019-9567issn
1098-5522journal_volume
47pub_type
杂志文章abstract::The complement system plays an important role in the host defense against infection, and the formation of the terminal complement complex on the bacterial surface has been shown to be particularly important in killing of gram-negative bacteria. The gram-negative periodontal pathogen Porphyromonas gingivalis is resista...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00304-06
更新日期:2006-09-01 00:00:00
abstract::Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacterial proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tube...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5628-5635.2005
更新日期:2005-09-01 00:00:00
abstract::Heterogeneic autoantibodies against axonal neurofilament proteins of mature mouse neurons grown in vitro were detected by the indirect immunofluorescence technique in 12.7% (9 of 71) of the sera from nonhuman primates infected with kuru, in 14.5% (17 of 117) and 4% (1 of 25), respectively, of the sera from nonhuman pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.316-324.1982
更新日期:1982-10-01 00:00:00
abstract::In vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal B-cell activators (PBA). We reasoned that if PBA were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.823-830.1988
更新日期:1988-04-01 00:00:00
abstract::We previously showed that the Uganda Palo Alto line of Plasmodium falciparum propagated in Saimiri monkeys and the line maintained in culture in human erythrocytes for many years in our laboratory are genetically unrelated (T. Fandeur, S. Bonnefoy, and O. Mercereau-Puijalon, Mol. Biochem. Parasitol. 47:167, 1991). Whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1390-1396.1992
更新日期:1992-04-01 00:00:00
abstract::Mycobacterium tuberculosis, the primary etiologic agent of tuberculosis, is the world's leading cause of death from a single infectious agent, and new vaccines and drugs to combat it are urgently needed. The major extracellular proteins of M. tuberculosis, which are released into its phagosome in macrophages, its host...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2321-2328.1997
更新日期:1997-06-01 00:00:00
abstract::A Mycobacterium tuberculosis strain disrupted in the AraC homologue Rv1931c was isolated. The mutant strain exhibited reduced survival both in macrophages and in a mouse infection model, with survival being restored on complementation with the Rv1931c gene. These results suggest that Rv1931c regulates genes important ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5483-5486.2004
更新日期:2004-09-01 00:00:00
abstract::The suspected correlation between cessation of type C virus production and halt in immunoglobulin secretion by murine myeloma cells was studied. Employing two variants of the murine myeloma MOPC-315, immunoglobulin A-producing and nonproducing cells, we demonstrated that the two myelomas release similar levels of type...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.569-573.1979
更新日期:1979-08-01 00:00:00
abstract::The leukotrienes and tumor necrosis factor (TNF) play an important role in the pathophysiology of septic shock, in which hypotension, leukopenia, thrombocytopenia, and hemoconcentration are observed. This study was performed to examine the effects of a 5-lipoxygenase inhibitor (AA-861), a selective leukotriene recepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2432-2437.1992
更新日期:1992-06-01 00:00:00
abstract::Streptococcus pneumoniae (the pneumococcus), a leading cause of bacterial disease, is most commonly carried in the human nasopharynx. Colonization induces inflammation that promotes the organism's growth and transmission. This inflammatory response is dependent on intracellular sensing of bacterial components that acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-15
更新日期:2015-08-01 00:00:00
abstract::To explore the mechanisms underlying the eosinophil-mediated inflammation of tropical pulmonary eosinophilia (TPE), bronchoalveolar lavage (BAL) fluid, serum, and supernatants from pulmonary and blood leukocytes (WBC) from patients with acute TPE (n = 6) were compared with those obtained from healthy uninfected indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1337-1342.2003
更新日期:2003-03-01 00:00:00
abstract::We examined 205 enterotoxigenic strains of Escherichia coli for colonization factor antigens (CFA) I and II, using an immunodiffusion technique with specific antisera. A total of 36 strains of serogroups O63, O78, O114, O128, and O153 and 1 rough strain possessed CFA/I and gave a single precipitin line; 47 strains of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.189-197.1982
更新日期:1982-04-01 00:00:00
abstract::The major outer membrane protein (P2) of Haemophilus influenzae type b (Hib) with an apparent molecular weight of 37,000 to 40,000 has been previously shown to function as a porin and also as a target for antibodies protective against experimental Hib disease. The gene encoding the Hib P2 protein was cloned by using a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2709-2716.1988
更新日期:1988-10-01 00:00:00
abstract::Studies of meningococcal pathogenesis have been severely restricted due to the absence of an adequate animal model. Given the significance of iron in meningococcal pathogenesis, we developed a model of Neisseria meningitidis colonization in outbred adult mice that included daily administration of iron dextran. While r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.1849-1855.2003
更新日期:2003-04-01 00:00:00
abstract::Protective immunity against Toxoplasma gondii is known to be mediated mainly by T lymphocytes and gamma interferon (IFN-gamma). The contribution of CD4(+) and CD8(+) T-lymphocyte subsets to protective immune responses against T. gondii infection, triggered by a GRA1 (p24) DNA vaccine, was assessed in this study. In vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.309-316.2003
更新日期:2003-01-01 00:00:00
abstract::Pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. P. multocida strains simultaneously express two lipopolysaccharide (LPS) glycoforms (glycoforms A and B) that differ only in their inner core structure. Glycoform A contains a single 3-deoxy-d-manno-octulosonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00457-10
更新日期:2010-09-01 00:00:00
abstract::A previously undescribed, immunoreactive, membranous spherule outer wall (SOW) fraction produced by Coccidioides immitis (strains 634 and 735) grown in culture was isolated. Both this fraction and intact spherules were reactive with sera from coccidioidomycosis patients, as demonstrated by immunofluorescence microscop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2686-2694.1988
更新日期:1988-10-01 00:00:00
abstract::The mig gene of Streptococcus dysgalactiae, a major bovine mastitis pathogen, encodes two plasma protein-binding receptors, alpha2-macroglobulin (alpha2-M) and immunoglobulin G (IgG). In this study, the mig gene from one S. dysgalactiae isolate was cloned and expressed in Escherichia coli. The IgG receptor region enco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6030-6037.2001
更新日期:2001-10-01 00:00:00
abstract::Hybridomas secreting monoclonal antibodies (MABs) specific for Clostridium perfringens type A enterotoxin were produced by fusion of P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with purified enterotoxin. Wells containing hybridomas secreting immunoglobulin G (IgG) antibodies against enterot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.442-448.1985
更新日期:1985-11-01 00:00:00
abstract::A total of 224 strains were serogrouped by coagglutination (COA) and serotyped by protein I enzyme-linked immunosorbent assay (ELISA). Of these strains, 61 were from patients with disseminated gonococcal infection, 21 were from patients with pelvic inflammatory disease, and 115 were from patients with uncomplicated go...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.229-239.1982
更新日期:1982-01-01 00:00:00
abstract::The results show that Listeria monocytogenes, Francisella tularensis, and Salmonella typhimurium are facultative intracellular bacteria with a capacity to invade and grow in nonphagocytic cells in vivo. In the liver, all of these pathogens were seen to invade and to multiply extensively in hepatocytes. In all three ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5164-5171.1992
更新日期:1992-12-01 00:00:00
abstract::The response to reinfection with Ethiopian Leishmania donovani was evaluated in genetically determined noncure (H-2d) B10.D2 mice that are able to resolve infection due to sublethal irradiation pretreatment after inoculation with a low parasite dose and in C57BL/10 mice that demonstrate the genetically determined cure...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2892-2899.1989
更新日期:1989-09-01 00:00:00
abstract::Mutation of tryptophan-101 in Clostridium difficile toxin A, a 308-kDa glucosyltransferase, resulted in a 50-fold-reduced cytopathic activity in cell culture experiments. The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00545-06
更新日期:2006-10-01 00:00:00
abstract::Bordetella pertussis, a causative agent of whooping cough, expresses BrkA, which confers serum resistance, but the closely related human pathogen that also causes whooping cough, Bordetella parapertussis, does not. Interestingly, B. parapertussis, but not B. pertussis, produces an O antigen, a factor shown in other mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01629-07
更新日期:2008-04-01 00:00:00
abstract::Attachment of Mycobacterium avium subsp. paratuberculosis to host tissue and penetration of mucosal surfaces are pivotal events in the pathogenesis of Johne's disease. Fibronectin (FN) binding is required for attachment and internalization of several mycobacteria by epithelial cells in vitro. The objective of this stu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2075-2082.2001
更新日期:2001-04-01 00:00:00
abstract::An effective vaccine for Vibrio cholerae is not yet available for use in the developing world, where the burden of cholera disease is highest. Characterizing the proteins that are expressed by V. cholerae in the human host environment may provide insight into the pathogenesis of cholera and assist with the development...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-08
更新日期:2008-09-01 00:00:00
abstract::Corynebacterium renale type I (strain 115), 1.7 X 10(7) to 4.5 X 10(7) organisms, introduced intravenously into mice disappeared from the blood less than 24 h after inoculation and did not produce pyelonephritis. The same strain, 1 X 10(7) to 5 X 10(7) organisms, inoculated into the urinary bladder of mice was not rec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.263-267.1977
更新日期:1977-04-01 00:00:00
abstract::The cell surface of Chlamydia psittaci seems important for establishing infection since (i) UV-treated elementary bodies (EB) attach to and are ingested by L cells and (ii) heat or antibody treatment decreases attachment to L cells and promotes the fusion of chlamydiae-containing phagosomes with lysosomes in macrophag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.741-751.1983
更新日期:1983-05-01 00:00:00
abstract::Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.798-803.1978
更新日期:1978-12-01 00:00:00
abstract::Chronic pulmonary infection with Pseudomonas aeruginosa is common in cystic fibrosis (CF) patients. P. aeruginosa lipopolysaccharide (LPS), phosholipase C (PLC), and exotoxin A (ETA) were evaluated for their ability to induce pulmonary inflammation in mice following intranasal inoculation. Both LPS and PLC induced hig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1352-1358.2002
更新日期:2002-03-01 00:00:00