Abstract:
:An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric enzyme with a molecular mass of 96 kDa and an isoelectric point of 4.8. Its activity was optimal at pH 7.0 at 40 degrees C. The enzyme was strongly inhibited by EDTA, 1,10-phenathroline, and divalent cations (Zn(2+) and Hg(2+)), suggesting that this protein was a metalloaminopeptidase. The enzyme showed preference for alanine at the N termini of aminoacyl derivatives. The K(m) values for L-alanine-p-nitroanilide (Ala-pNA) and Lys-pNA were 0.35 and 0.18 mM, respectively. The N-terminal sequence of aminopeptidase was used for a homologous search in the genomes of B. melitensis 16M and Brucella suis 1330. The analysis revealed an exact match of the probe sequence (36 bp) with an open reading frame of 2,652 bp encoding a protein predicted to be alanyl aminopeptidase (aminopeptidase N). Collectively, these data suggest designation of the B. melitensis enzyme as an aminopeptidase N. The aminopeptidase was recognized by sera from patients with acute and chronic brucellosis, suggesting that the enzyme may have important diagnostic implications.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Contreras-Rodriguez A,Ramirez-Zavala B,Contreras A,Schurig GG,Sriranganathan N,Lopez-Merino Adoi
10.1128/iai.71.9.5238-5244.2003keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
5238-44issue
9eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::The attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis was examined in vitro. The attachment was inhibited by preincubation of granulosa cells with anti-chicken fibronectin antibody (approximately 70% reduction in attachment) or preincubation with a 14-kDa fimbrial protein isolated fr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5015-5021.1996
更新日期:1996-12-01 00:00:00
abstract::Two distinct monoclonal antibodies, one to pertussis toxin subunit S2, called 9G8, and another to subunits S2 and S3, called 11E6, were generated from the hybridomas of myeloma SP2/0 and spleen cells of BALB/c mice immunized mainly with the subunit S234 complex. Binding ability of 9G8 and 11E6 to the subunits was conf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.4.909-915.1987
更新日期:1987-04-01 00:00:00
abstract::Nucleotide-binding oligomerization domain (NOD) protein 1 (NOD1) and NOD2 are pathogen recognition receptors that sense breakdown products of peptidoglycan (PGN) (muropeptides). It is shown that a number of these muropeptides can induce tumor necrosis factor alpha (TNF-alpha) gene expression without significant TNF-al...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01597-06
更新日期:2007-02-01 00:00:00
abstract::Adherence of blastoconidia to epidermal corneocytes is an early event in Candida colonization and infection of the skin. Pathogenic species adhere more avidly than nonpathogenic species, transform to hyphal growth, and invade the stratum corneum of the skin. Adherence was studied by scanning electron microscopy of exp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1942-1949.1988
更新日期:1988-08-01 00:00:00
abstract::Tissue macrophages are the obligatory host cells for Leishmania donovani, the causative agent of visceral leishmaniasis. In this study we sought to determine whether activated macrophages, as defined by the functional criterion of tumor cell cytotoxicity, were also able to exert a microbicidal effect on ingested L. do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.918-926.1981
更新日期:1981-09-01 00:00:00
abstract::Alpha-2,3-sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to complement-mediated killing. The enzyme is constitutively expressed from a single gene (lst) and does not undergo antigen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2637-2650.2006
更新日期:2006-05-01 00:00:00
abstract::Anaplasma marginale subsp. centrale was the first vaccine used to protect against a rickettsial disease and is still in widespread use a century later. As its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by sequential passage among donor animals, ex...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00199-13
更新日期:2013-05-01 00:00:00
abstract::Five monoclonal antibodies (MAbs) were partially characterized and tested for their ability to inhibit penetration of Madin-Darby bovine kidney (MDBK) cells by sporozoites of Eimeria bovis. By indirect fluorescent-antibody assays, all MAbs reacted with acetone-fixed sporozoites, but only two MAbs, EbS9 (immunoglobulin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2538-2543.1988
更新日期:1988-10-01 00:00:00
abstract::Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-soluble toxin molecules bind to cholesterol-containing target membranes to assemble into supramolecular curved rod structures (25 to 1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.52-60.1985
更新日期:1985-01-01 00:00:00
abstract::Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative sensitivity of the two culture systems for detection of the infection. The strains tested were both yolk sac- and HeLa cell-esta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.1078-1082.1975
更新日期:1975-11-01 00:00:00
abstract::Since the pH of the gingival crevice increases from below neutrality in health to above pH 8 in disease, we decided to investigate the effect of environmental pH on the growth and enzyme activity of Bacteroides gingivalis W50. Cells were grown in a chemostat under hemin-excess conditions over a range of pH values; sta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1096-1100.1988
更新日期:1988-05-01 00:00:00
abstract::Cytokine gene expression in cells migrating in afferent and efferent intestinal lymph was monitored for extended time periods in individual sheep experimentally infected with the nematode Trichostrongylus colubriformis. Animals from stable selection lines with increased levels of either genetic resistance (R) or susce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2175-2183.2005
更新日期:2005-04-01 00:00:00
abstract::The macrophage has previously been implicated in contributing to the renal inflammation associated with hemolytic-uremic syndrome (HUS). However, there is currently no in vivo model detailing the contribution of the renal macrophage to the kidney disease associated with HUS. Therefore, renal macrophage recruitment and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01663-06
更新日期:2007-03-01 00:00:00
abstract::CC chemokine receptor 4 (CCR4) and its two ligands, CCL17 and CCL22, are critically involved in different immune processes. In models of lipopolysaccharide-induced shock, CCR4-deficient (CCR4(-/-)) mice showed improved survival rates associated with attenuated proinflammatory cytokine release. Using CCR4(-/-) mice wit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00310-08
更新日期:2008-11-01 00:00:00
abstract::The effects of temperature and treatment with sodium dodecyl sulfate, Tween 20, dithiothreitol, trypsin, or guanidine on the hemagglutinating capacity of six strains of type A influenza virus (A(0)/PR8/34, A(1)/CAME/46, A(2)/J305/57, A(2)/Bethesda/63, A(2)/HK/Aichi/68, and A(2)/HK/80/68), one strain of swine virus (A/...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.3.324-331.1972
更新日期:1972-03-01 00:00:00
abstract::The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2587-2594.1998
更新日期:1998-06-01 00:00:00
abstract::Pneumolysin (PLY), an important virulence factor of Streptococcus pneumoniae, is known to exert various effects on the host immune cells, including cytokine induction, in addition to its known cytolytic activity as a member of the thiol-activated cytolysins. It is of interest to determine whether cytolytic activity is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.107-113.2002
更新日期:2002-01-01 00:00:00
abstract::Groups of mice were immunized either subcutaneously or intranasally with purified Brucella melitensis lipopolysaccharide (LPS) or with LPS as a noncovalent complex with Neisseria meningitidis group B outer membrane protein (LPS-GBOMP). Control mice were inoculated with sterile saline. Two doses of vaccine were given 4...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00331-06
更新日期:2006-10-01 00:00:00
abstract::Citrate is a ubiquitous compound and can be utilized by many bacterial species, including enteric pathogens, as a carbon and energy source. Genes involved in citrate utilization have been extensively studied in some enteric bacteria, such as Klebsiella pneumoniae; however, their role in pathogenesis is still not clear...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00746-18
更新日期:2019-02-21 00:00:00
abstract::Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser des...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5905-5907.2001
更新日期:2001-09-01 00:00:00
abstract::LT-IIa and LT-IIb, the type II heat-labile enterotoxins of Escherichia coli, are closely related in structure and function to cholera toxin and LT-I, the type I heat-labile enterotoxins of Vibrio cholerae and E. coli, respectively. Recent studies from our group demonstrated that LT-IIa and LT-IIb are potent systemic a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1330-1342.2005
更新日期:2005-03-01 00:00:00
abstract::Attachment of Mycobacterium avium subsp. paratuberculosis to host tissue and penetration of mucosal surfaces are pivotal events in the pathogenesis of Johne's disease. Fibronectin (FN) binding is required for attachment and internalization of several mycobacteria by epithelial cells in vitro. The objective of this stu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2075-2082.2001
更新日期:2001-04-01 00:00:00
abstract::Immunochemical analyses of soluble antigens derived from microaerophilous stationary phase cultures of Babesia bovis demonstrated that at least three parasite antigens were released in vitro. These antigens have molecular weights within the range of 37,000 to 40,000, fast electrophoretic mobility in the albumin and al...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.358-361.1981
更新日期:1981-01-01 00:00:00
abstract::Effector mechanisms resulting from alternative complement pathway (ACP) activation cannot act efficiently against Yersinia enterocolitica serotype O3, as indicated by poor C3 to C9 consumption and by survival in EGTA (ethyleneglycoldiaminetetraacetic acid) Mg-serum. These results were not influenced by the lack or pre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.1984-1989.1989
更新日期:1989-07-01 00:00:00
abstract::The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3' specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.673-679.1994
更新日期:1994-02-01 00:00:00
abstract::The mechanisms that govern the initial interaction between Paracoccidioides brasiliensis, a primary dimorphic fungal pathogen, and cells of the innate immunity need to be clarified. Our previous studies showed that Toll-like receptor 2 (TLR2) and TLR4 regulate the initial interaction of fungal cells with macrophages a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00375-10
更新日期:2011-06-01 00:00:00
abstract::To determine the ability of gut antigens to induce a protective immune response against blood-feeding nematodes, isolated gut antigens were used to immunize goats against Haemonchus contortus. Immunization-induced antibody responses recognized parasite gut antigens which were associated predominantly with the microvil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4412-4417.1991
更新日期:1991-12-01 00:00:00
abstract::Mycoplasma penetrans adhered to cultured human cells, forming clusters that localized to specific areas of the host cell surface. Adherence and cluster formation were inhibited by anti-M. penetrans antibodies, suggesting the involvement of specific adhesin-receptor interactions. Ultrastructural studies showed that aft...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.197-208.1996
更新日期:1996-01-01 00:00:00
abstract::Borrelia burgdorferi undergoes differential gene expression during transmission from its tick vector to a vertebrate host. The addition of blood to a spirochete culture at 35 degrees C for 48 h had a dramatic effect on gene expression of this organism. Utilizing B. burgdorferi whole genome DNA arrays, we compared the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5419-5432.2004
更新日期:2004-09-01 00:00:00
abstract::The antigenic relationship of six different strains of Paracoccidioides brasiliensis was investigated by the agar-gel immunodiffusion test. Soluble antigens prepared from the two morphological phases of the fungus were diffused against human sera obtained from eight patients with paracoccidioidomycosis (South American...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.3.268-273.1970
更新日期:1970-09-01 00:00:00