Abstract:
:Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser desorption ionization and nano-electrospray mass spectrometry.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Jungblut PR,Müller EC,Mattow J,Kaufmann SHdoi
10.1128/iai.69.9.5905-5907.2001keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
5905-7issue
9eissn
0019-9567issn
1098-5522journal_volume
69pub_type
杂志文章abstract::The gene products from an 8-kb region adjacent to the 3' end of the ptx operon are required by Bordetella pertussis for the export of pertussis holotoxin. At least one of these gene products (PtlC) is specifically required for the export of assembled holotoxin from the periplasmic space. ptlC mutants exhibit a 20-fold...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3920-3926.1995
更新日期:1995-10-01 00:00:00
abstract::Helicobacter pylori infection causes severe gastroduodenal diseases in humans. Its virulence is strongly increased by the presence of the cag pathogenicity island (cag PAI). It has been shown that CagA, a major antigen in humans, is translocated to the host cell via a secretion system encoded by the cag PAI. The roles...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6468-6470.2002
更新日期:2002-11-01 00:00:00
abstract::Purified beta-toxin from Clostridium perfringens type C was found to be a single polypeptide chain protein with a molecular weight of approximately 30,000. The toxin was heat labile, with 75% of its activity being inactivated by incubation at 50 degrees C for 5 min. Biological activity of the purified toxin was comple...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.678-680.1978
更新日期:1978-08-01 00:00:00
abstract::The effects of infection with Rowson-Parr virus (RPV), an associated virus present in Friend virus (FV) preparations, on the splenic plaque-forming cell response to sheep red cells in adult mice and on the plaque-forming cell response of peritoneal cells in vitro have been investigated. The results support the hypothe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.2.160-166.1973
更新日期:1973-02-01 00:00:00
abstract::The Plasmodium falciparum liver-stage antigen 3 (LSA3), a recently identified preerythrocytic antigen, induces protection against malaria in chimpanzees. Using antibodies from individuals with hyperimmunity to malaria affinity purified on recombinant or synthetic polypeptides of LSA3, we identified four non-cross-reac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3845-3952.2001
更新日期:2001-06-01 00:00:00
abstract::Recognition of microbial patterns by host receptors is the first step in a multistep sequence leading to neutrophil-dependent host resistance. Although the role of membrane-bound sensors in bacterial recognition has been examined in detail, the importance of cytosolic sensors in the lungs is largely unexplored. In thi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05641-11
更新日期:2011-11-01 00:00:00
abstract::El Tor and non-O1 strains of Vibrio cholerae were analyzed to determine whether synthesis of secreted hemolysin was influenced by the concentration of iron in the medium. Synthesis of hemolysin was found to be iron regulated in both El Tor and non-O1 isolates. Increased levels of hemolytic activity were detected in su...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.11.2891-2895.1988
更新日期:1988-11-01 00:00:00
abstract::We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We mea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.971-977.1981
更新日期:1981-03-01 00:00:00
abstract::Bordetella pertussis was grown in iron (Fe)-free defined medium to limit the growth of the organism. Doubling times of the Fe-starved organism increased by approximately 1 h, and a 40% reduction in the final extent of growth in Fe-depleted medium was observed. Under these conditions, a hydroxamate siderophore named bo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.117-123.1992
更新日期:1992-01-01 00:00:00
abstract::Indoleamine 2,3-dioxygenase (IDO), a tryptophan-degrading enzyme, is inducible by various interferons (IFNs). IDO-mediated tryptophan degradation, but not the formation of IDO-catalyzed tryptophan metabolites, has been suggested as a mechanism for the antiparasitic action of IFN-gamma. To determine whether the IFN-gam...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.1810-1813.1993
更新日期:1993-05-01 00:00:00
abstract::Interaction of bacteria with mucosal surfaces can modulate the production of proinflammatory cytokines and adhesion molecules produced by epithelial cells. Previously, we showed that expression of interleukin-8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) by gingival epithelial cells increases following inter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1364-1372.2001
更新日期:2001-03-01 00:00:00
abstract::Ambient pH signaling involves a cascade of conserved Rim or Pal products in ascomycetous yeasts or filamentous fungi, respectively. Recent evidences in the fungi Aspergillus nidulans, Saccharomyces cerevisiae, Yarrowia lipolytica, and Candida albicans suggested that components of endosomal sorting complexes required f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.7977-7987.2005
更新日期:2005-12-01 00:00:00
abstract::Mycoplasma arthritidis induces an acute to chronic arthritis in rodents. Arthritis induced in mice histologically resembles human rheumatoid arthritis and can be associated with lethal toxicity following systemic injection. The M. arthritidis mitogen (MAM) superantigen has long been implicated as having a role in path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00667-08
更新日期:2008-11-01 00:00:00
abstract::Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate effectors called Yersinia outer proteins (Yops) into infected host cells. Previous studies demonstrated a role for effector Yops in the inhibition of caspase-1-mediated cell death and secretion of interleukin-1beta (IL-1beta) in naïv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01695-07
更新日期:2008-09-01 00:00:00
abstract::Humoral and cellular immune responses to several antigens were compared in control and hypercholesterolemic groups of monkeys. Chronic hypercholesterolemia, with concomitant hyperphospholipidemia and hypotriglyceridemia, was produced experimentally by feeding monkeys a high-fat, high-cholesterol diet. When studied pri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.1.105-109.1973
更新日期:1973-07-01 00:00:00
abstract::Only 20 to 40% of human blood monocytes were capable of responding to chemotaxins in vitro. This limit is not due to restrictions of the in vitro system, but is due to the existence of a migrating subpopulation. Over a wide range, the number of cells migrating toward a given concentration of chemotaxin was directly pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::The American opossum is the only experimental animal that regularly develops bacterial endocarditis spontaneously. There was no relation between the ability of opossums to clear bacteria from the bloodstream and the subsequent development of endocarditis. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.6.1126-1128.1974
更新日期:1974-06-01 00:00:00
abstract::We examined the reactivities of Clostridium butyricum neurotoxin to nine monoclonal antibodies against Clostridium botulinum type E neurotoxin which recognize the light chain or the amino-terminal half (H-1 fragment) or the carboxyl-terminal half (H-2 fragment) of the heavy chain of botulinum neurotoxin. Butyricum neu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.457-459.1991
更新日期:1991-01-01 00:00:00
abstract::The adsorption of (3)H-labeled Streptococcus mutans 6715 cells to disks of hydroxyapatite (HA) was studied. The number of streptococci that adsorbed was logarithmically related to the concentration of cells available up to at least 2 x 10(8) per ml; equilibrium occurred within 45 min. Assay reliability was verified by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.514-523.1977
更新日期:1977-11-01 00:00:00
abstract::Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacterial proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tube...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5628-5635.2005
更新日期:2005-09-01 00:00:00
abstract::A novel immunobiologically active fraction was prepared from a phenol-water extract of Prevotella intermedia ATCC 25611 by Sephadex G-100 column chromatography. The fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. The fraction showed high-molecular-weight bands (10,000 to 12,000) on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4531-4538.1997
更新日期:1997-11-01 00:00:00
abstract::The in vitro effects of Pseudomonas aeruginosa elastase (P. aeruginosa E) and of human leukocyte elastase on human airway lysozyme (HAL) were investigated. P. aeruginosa E inactivated and cleaved HAL, whereas human leukocyte elastase had no effect. Total inactivation of HAL by P. aeruginosa E was observed after 120 mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.2.555-560.1985
更新日期:1985-02-01 00:00:00
abstract::The inactivation of Coxiella burnetii in suspension or in cultures of guinea pig peritoneal macrophages by ultraviolet (UV) light was studied. The effect of UV treatment on the activity of macrophage organelle marker enzymes and their subsequent equilibration in linear sucrose gradients was also determined. It was sho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.837-841.1980
更新日期:1980-03-01 00:00:00
abstract::Increased resistance to infection with Brucella abortus 2308 resulted when recombinant murine gamma interferon (rMuIFN-gamma) was given to mice both before and during infection but not when given only before infection. Mice given rMuIFN-gamma had enhanced peritoneal and splenic macrophage bactericidal activity against...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4407-4409.1992
更新日期:1992-10-01 00:00:00
abstract::Alpha-2,3-sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to complement-mediated killing. The enzyme is constitutively expressed from a single gene (lst) and does not undergo antigen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2637-2650.2006
更新日期:2006-05-01 00:00:00
abstract::Thirteen Habs-serotype strains could be classified into 11 groups depending on the characteristic patterns of 2-amino sugar composition; strains of serotypes 2, 5, and 7 had the same pattern, and each other serotype strain had its own distinctive pattern. In our classification, fucosamine and quinovosamine were of imp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.692-697.1977
更新日期:1977-03-01 00:00:00
abstract::Enterotoxigenic Escherichia coli (ETEC) is a major cause of traveler's diarrhea as well as of endemic diarrhea and stunting in children in developing areas. However, a small-mammal model has been badly needed to better understand and assess mechanisms, vaccines, and interventions. We report a murine model of ETEC diar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00183-18
更新日期:2018-06-21 00:00:00
abstract::In this study we used Escherichia coli strain F11(P155) of porcine origin. The heat-stable enterotoxin (ST) was produced with a batch fermentor under agitation (500 rpm) and forced aeration (5 liters/min) in Casamino Acids yeast extract medium containing 0.2% glucose. The pH varied from 7.2 to 7.8. The maximum amount ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Monolayer cultures of LLC-MK2 rhesus monkey kidney cells become persistently infected with simian virus 40 after infection at input multiplicities of 100, 10, or 1 plaque-forming unit per cell. After 3 weeks, all cells of the cultures infected at a multiplicity of 1 plaque-forming unit per cell produced the simian vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.868-871.1977
更新日期:1977-12-01 00:00:00
abstract::Antimicrobial fatty acids (AFAs) protect the human epidermis against invasion by pathogenic bacteria. In this study, we questioned whether human skin fatty acids (FAs) can be incorporated into the lipid moiety of lipoproteins and whether such incorporation would have an impact on innate immune stimulation in the model...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00822-15
更新日期:2015-10-26 00:00:00