Abstract:
:Ambient pH signaling involves a cascade of conserved Rim or Pal products in ascomycetous yeasts or filamentous fungi, respectively. Recent evidences in the fungi Aspergillus nidulans, Saccharomyces cerevisiae, Yarrowia lipolytica, and Candida albicans suggested that components of endosomal sorting complexes required for transport (ESCRT) involved in endocytic trafficking were needed for signal transduction along the Rim pathway. In this study, we confirm these findings with C. albicans and show that Vps28p (ESCRT-I) and Vps32p/Snf7p (ESCRT-III) are required for the transcriptional regulation of known targets of the Rim pathway, such as the PHR1 and PHR2 genes encoding cell surface proteins, which are expressed at alkaline and acidic pH, respectively. We additionally show that deletion of these two VPS genes, particularly VPS32, has a more drastic effect than a RIM101 deletion on growth at alkaline pH and that this effect is only partially suppressed by expression of a constitutively active form of Rim101p. Finally, in an in vivo mouse model, both vps null mutants were significantly less virulent than a rim101 mutant, suggesting that VPS28 and VPS32 gene products affect virulence both through Rim-dependent and Rim-independent pathways.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cornet M,Bidard F,Schwarz P,Da Costa G,Blanchin-Roland S,Dromer F,Gaillardin Cdoi
10.1128/IAI.73.12.7977-7987.2005keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
7977-87issue
12eissn
0019-9567issn
1098-5522pii
73/12/7977journal_volume
73pub_type
杂志文章abstract::gammaG-globulin from nonimmunized rabbits and from rabbits immunized with various bacteria reacted in the immunofluorescence technique with protein A-containing Staphylococcus aureus. Pepsin digestion of most immunoglobulin preparations eliminated the reaction, thus showing that the Fc fragment is involved and that th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.4.387-391.1970
更新日期:1970-10-01 00:00:00
abstract::Virulent and avirulent clones of Leishmania donovani promastigotes were examined for their acid phosphatase activity. The acid phosphatase activity of whole-cell lysates of virulent clones was 1.5 to 2.0 times higher than that of avirulent clones. Pellet fractions (260,000 x g, 30 min) from sonicated promastigotes of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.11.2856-2860.1988
更新日期:1988-11-01 00:00:00
abstract::Lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age-groups is urgent. Concerns ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-20
更新日期:2020-12-14 00:00:00
abstract::The immune mechanism by which hamsters acquire resistance to infection with Treponema pertenue, the causative agent of frambesia, or yaws, has not been elucidated. Serum or cells (spleen or lymph node) obtained from hamsters resistant to frambesial infection were transferred to normal syngenic recipients, who are subs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.430-435.1978
更新日期:1978-08-01 00:00:00
abstract::Pathogenic leptospires have the ability to survive and disseminate to multiple organs after penetrating the host. Several pathogens, including spirochetes, have been shown to express surface proteins that interact with the extracellular matrix (ECM). This adhesin-mediated binding process seems to be a crucial step in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00460-06
更新日期:2006-11-01 00:00:00
abstract::Individuals in areas of Plasmodium falciparum endemicity develop immunity to malaria after repeated exposure. Knowledge of the acquisition and nature of protective immune responses to P. falciparum is presently limited, particularly for young children. We examined antibodies (IgM, IgG, and IgG subclasses) to merozoite...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02398-14
更新日期:2015-02-01 00:00:00
abstract::Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In chickens, the differential biology of Salmonella serovars (systemic versus gut-restricted localization) correlates with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2344-2350.2005
更新日期:2005-04-01 00:00:00
abstract::Infection of C57BL/6 mice with the third-stage larvae of Brugia pahangi results in a rapid expansion of NK1.1(+) T cells in the spleen and draining lymph nodes. NK T cells produced interleukin-4 in the spleen within 24 h of infection, and these cells were CD4(-). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2215-2219.2002
更新日期:2002-04-01 00:00:00
abstract::Antigen preparations derived from Plasmodium falciparum-infected erythrocytes (but not from uninfected erythrocytes) can stimulate the in vitro proliferation of peripheral blood lymphocytes from malaria-sensitized as well as nonsensitized donors. The possibility that the nonspecific responses might be due to a parasit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.106-110.1979
更新日期:1979-04-01 00:00:00
abstract::Plasmodium falciparum's ability to invade erythrocytes is essential for its survival within the human host. Immune mechanisms that impair this ability are therefore expected to contribute to immunity against the parasite. Plasma of humans who are naturally exposed to malaria has been shown to have growth-inhibitory ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06190-11
更新日期:2012-05-01 00:00:00
abstract::Broth culture supernatants from Tox+ Helicobacter pylori strains induce vacuolation of HeLa cells in vitro and contain VacA in concentrations that are higher than those found in supernatants from Tox- H. pylori strains. To investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cyto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3088-3094.1998
更新日期:1998-07-01 00:00:00
abstract::CS31A is a K88-related capsule-like surface protein that mediates Escherichia coli and Klebsiella pneumoniae adhesion to the human Caco-2 and Intestine-407 cell lines. In this study, we demonstrate that ClpG, the major subunit of CS31A, contains the adhesive domain of the polymerized structure. We mapped this domain w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.609-616.1997
更新日期:1997-02-01 00:00:00
abstract::Macrophage recognition of Salmonella enterica serovar Typhimurium leads to a cascade of signaling events, including the activation of Src family and Syk kinases and the production of reactive oxygen species (ROS), which are critical for host innate defense during early stages of bacterial infection. ROS production dep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00115-08
更新日期:2008-06-01 00:00:00
abstract::Active immunization of mice with high doses of heat-killed and autoclaved vaccine of unencapsulated strains of Staphylococcus aureus, which was grown in brain heart infusion media, protected against challenge with the Smith diffuse strain of Staphylococcus aureus. These organisms were capable of absorbing the protecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.939-942.1975
更新日期:1975-11-01 00:00:00
abstract::The interactions between Capnocytophaga ochracea ATCC 33596 and Streptococcus sanguis H1, Actinomyces naeslundii PK984, or Actinomyces israelii PK16 are dependent on specific recognitions between heat-sensitive adhesins on C. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.5.1198-1202.1987
更新日期:1987-05-01 00:00:00
abstract::Interaction of bacteria with mucosal surfaces can modulate the production of proinflammatory cytokines and adhesion molecules produced by epithelial cells. Previously, we showed that expression of interleukin-8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) by gingival epithelial cells increases following inter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1364-1372.2001
更新日期:2001-03-01 00:00:00
abstract::T cell-independent antibody responses develop rapidly, within 3 to 4 days, and are critical for preventing blood-borne pathogens from evolving into life-threatening infections. The interaction of BAFF, also known as BLyS, with its receptors BAFFR and TACI on B cells is critical for B cell homeostasis and function. Usi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01147-13
更新日期:2014-01-01 00:00:00
abstract::Suitability of the rhesus monkey (Macaca mulatta) as an experimental host for evaluation of vaccines against airborne infection with Mycobacterium tuberculosis strain H37Rv was investigated. Nonvaccinated monkeys were exposed to estimated doses of 12, 25, or 49 units of H37Rv in a modified Henderson apparatus, and the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.574-582.1970
更新日期:1970-11-01 00:00:00
abstract::The polysaccharide antigen preparations from serotype a and serotype d strains of Streptococcus mutans contained both a serotype-specific antigenic determinant and a common a-d antigenic determinant, as demonstrated by agar gel diffusion studies and a quantitative cross-precipitin assay. The chromatographically purifi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.791-798.1975
更新日期:1975-10-01 00:00:00
abstract::Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA gene, required for full urease activity, encodes a cytoplasmic membrane nickel transporter that imports nickel ions and le...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.685-691.2002
更新日期:2002-02-01 00:00:00
abstract::Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The consequences of vaccination were evaluated in terms of the growth of BCG in the lungs and spleen, lung and spleen weigh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Investigating the innate immune response mediators released in milk has manifold implications, spanning from elucidation of the role played by mammary epithelial cells (MECs) in fighting microbial infections to the discovery of novel diagnostic markers for monitoring udder health in dairy animals. Here, we investigate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-13
更新日期:2013-09-01 00:00:00
abstract::Localized juvenile periodontitis (LJP) runs in families, and a predisposition to develop disease appears to be inherited in an autosomal dominant fashion. Patients with LJP have elevated levels of serum immunoglobulin G2 (IgG2), and this is most striking in black LJP patients. We hypothesized that the markedly elevate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2004-2009.1996
更新日期:1996-06-01 00:00:00
abstract::Staphylococcus aureus is one of the pathogens most frequently isolated in device-related infections. S. aureus is equipped with surface-associated proteins promoting specific binding to matrix molecules. Clumping factor A (ClfA, encoded by clfA) mediates adhesion to fibrinogen. Whereas the contribution of ClfA to path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2758-2762.2002
更新日期:2002-06-01 00:00:00
abstract::Vibrio parahaemolyticus is the leading cause of bacterial seafood-borne gastroenteritis worldwide, yet little is known about how this pathogen colonizes the human intestine. The alternative sigma factor RpoN/sigma-54 is a global regulator that controls flagellar synthesis, as well as a wide range of nonflagellar genes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01210-13
更新日期:2014-02-01 00:00:00
abstract::The virulence of Candida albicans in a mouse model of invasive candidiasis is dependent on the phospholipids phosphatidylserine (PS) and phosphatidylethanolamine (PE). Disruption of the PS synthase gene CHO1 (i.e., cho1Δ/Δ) eliminates PS and blocks the de novo pathway for PE biosynthesis. In addition, the cho1Δ/Δ muta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01612-14
更新日期:2014-10-01 00:00:00
abstract::The diarrheagenic pathogen enteropathogenic Escherichia coli (EPEC) is responsible for significant infant mortality and morbidity, particularly in developing countries. EPEC pathogenesis relies on a type III secretion system-mediated transfer of virulence effectors into host cells. EPEC modulates host cell survival an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01690-06
更新日期:2007-05-01 00:00:00
abstract::Survival of macrophage microbicidal activity is a prerequisite for invasive disease caused by the enteric pathogen Salmonella enterica serovar Typhimurium. Flavohemoglobins, such as those of Escherichia coli, Salmonella, and yeast, play vital roles in protection of these microorganisms in vitro from nitric oxide (NO) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4399-4405.2002
更新日期:2002-08-01 00:00:00
abstract::Enteropathogenic Escherichia coli (EPEC) interacts with intestinal epithelial cells, activating host signaling pathways leading to cytoskeletal rearrangements and ultimately diarrhea. In this study, we demonstrate that EPEC interacts with the macrophage-like cell line J774A.1 to inhibit phagocytosis by these cells. An...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.490-495.1999
更新日期:1999-02-01 00:00:00
abstract::Mouse fibroblasts (L cells) infected with the 6BC strain of Chlamydia psittaci released potassium ion (K(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. When the multiplicity of infection was 500 to 1,000 50% infectious units (ID(50)) per L cell, loss of intracell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.827-832.1978
更新日期:1978-03-01 00:00:00