Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay.

Abstract:

:The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3' specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tissue from 10 children who were clinically infection free but were having their adenoids removed because of nasal obstruction. In some cases, the reticular crypt epithelium was focally infiltrated by H. influenzae. The reservoir for these bacterial colonizations, in all likelihood long standing, seemed to be macrophage-like cells found in the subepithelial layers in all 10 cases. These mononuclear cells contained up to 200 intracellular H. influenzae cells. In the transmission electron macroscope, macrophage-like cells with intracellular bacteria with coccoid morphology, at least some of which were dividing, were seen. Adenoid cell suspensions, enriched for macrophages by use of paramagnetic beads coated with monoclonal antibodies against the CD14 marker, yielded up to 1,100 CFU of nontypeable H. influenzae per 10(5) cells after killing of extracellular bacteria with gentamicin followed by mechanical lysis of the cells.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Forsgren J,Samuelson A,Ahlin A,Jonasson J,Rynnel-Dagöö B,Lindberg A

doi

10.1128/IAI.62.2.673-679.1994

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

673-9

issue

2

eissn

0019-9567

issn

1098-5522

journal_volume

62

pub_type

杂志文章
  • Legionella pneumophila type II protein secretion promotes virulence in the A/J mouse model of Legionnaires' disease pneumonia.

    abstract::Legionella pneumophila, the gram-negative agent of Legionnaires' disease, possesses type IV pili and a type II protein secretion (Lsp) system, both of which are dependent upon the PilD prepilin peptidase. By analyzing multiple pilD mutants and various types of Lsp mutants as well as performing trans-complementation of...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.72.1.310-321.2004

    authors: Rossier O,Starkenburg SR,Cianciotto NP

    更新日期:2004-01-01 00:00:00

  • Viral spread in the presence of neutralizing antibody: mechanisms of persistence in foamy virus infection.

    abstract::Several viruses were categorized on the basis of their ability to spread from cell to contiguous cell and form plaques in the presence of antiviral antibody. Herpes simplex virus, cytomegalovirus, and vaccinia, measles, and foamy viruses were able to spread in the presence of neutralizing antibody, whereas coxsackievi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.14.5.1172-1178.1976

    authors: Hooks JJ,Burns W,Hayashi K,Geis S,Notkins AL

    更新日期:1976-11-01 00:00:00

  • Role of Escherichia coli alpha-hemolysin and bacterial adherence in infection: requirement for release of inflammatory mediators from granulocytes and mast cells.

    abstract::We investigated the role of bacterial mannose-resistant fimbriation of S fimbriae (Fim), mannose-resistant hemagglutination (S-Mrh), and hemolysin (Hly) production by an Escherichia coli parent and genetically cloned strains as regards their effect on histamine release from rat mast cells and generation of the chemilu...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.54.3.886-892.1986

    authors: König B,König W,Scheffer J,Hacker J,Goebel W

    更新日期:1986-12-01 00:00:00

  • Entry of a recombinant, full-length, atoxic tetanus neurotoxin into Neuro-2a cells.

    abstract::Tetanus neurotoxin (TeNT) and botulinum neurotoxin (BoNT) are clostridial neurotoxins (CNTs) responsible for the paralytic diseases tetanus and botulism, respectively. CNTs are AB toxins with an N-terminal zinc-metalloprotease light chain that is linked by a disulfide bond to a C-terminal heavy chain that includes a t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01539-13

    authors: Blum FC,Przedpelski A,Tepp WH,Johnson EA,Barbieri JT

    更新日期:2014-02-01 00:00:00

  • Bradyzoite-specific surface antigen SRS9 plays a role in maintaining Toxoplasma gondii persistence in the brain and in host control of parasite replication in the intestine.

    abstract::Toxoplasma gondii is a ubiquitous parasite that persists for the life of a healthy mammalian host. A latent, chronic infection can reactivate upon immunosuppression and cause life-threatening diseases, such as encephalitis. A key to the pathogenesis is the parasite's interconversion between the tachyzoite (in acute in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01862-06

    authors: Kim SK,Karasov A,Boothroyd JC

    更新日期:2007-04-01 00:00:00

  • Mechanism of impaired iron release by the reticuloendothelial system during the hypoferremic phase of experimental Neisseria meningitidis infection in mice.

    abstract::Hypoferremia, the reduction of plasma transferrin iron levels during infection, has been shown to control Neisseria meningitidis infection in mice. The exact nature of the mechanism that regulates this response has been obscure. We have previously shown that hypoferremia does not result from an accelerated removal of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.44.2.320-325.1984

    authors: Letendre ED,Holbein BE

    更新日期:1984-05-01 00:00:00

  • Paracoccidioides brasiliensis-stimulated human gamma/delta T cells support antibody production by B cells.

    abstract::Paracoccidioidomycosis patients show hyperactive humoral immune responses. Consequently, we investigated whether cytokines in supernatants from Paracoccidioides brasiliensis-stimulated gamma/delta T cells support B-cell activation. We detected proliferation of B cells and increased immunoglobulin M (IgM) and IgG produ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.4.1608-1610.1995

    authors: Munk ME,Fazioli RA,Calich VL,Kaufmann SH

    更新日期:1995-04-01 00:00:00

  • Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin.

    abstract::The in vitro effects of pneumolysin, a sulfhydryl-activated toxin produced by Streptococcus pneumoniae, on various functions of human polymorphonuclear leukocytes (PMNLs) was investigated. Treatment of PMNLs with highly purified toxin significantly inhibited respiratory burst (in response to stimulation), ability to k...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.41.3.1212-1216.1983

    authors: Paton JC,Ferrante A

    更新日期:1983-09-01 00:00:00

  • Effect of antimacrophage serum on dermal tuberculin sensitivity and allergic pulmonary granuloma formation in rabbits.

    abstract::Anti-rabbit alveolar macrophage goat serum (AMS), adsorbed of hemolysins and hemagglutinins, exhibited complement-dependent cytotoxicity for the following rabbit target cells: alveolar macrophages, thymocytes, and kidney (RK-13) cells. The intravenous injection of AMS, sufficient to reduce markedly the dermal tubercul...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.2.6.810-814.1970

    authors: Moore VL,Myrvik QN

    更新日期:1970-12-01 00:00:00

  • Interaction of murine cytomegalovirus with separated populations of spleen cells.

    abstract::Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.26.3.853-860.1979

    authors: Loh L,Hudson JB

    更新日期:1979-12-01 00:00:00

  • Characterization of GP110, a neutrophil surface protein.

    abstract::Localized juvenile periodontosis is associated with a defect of neutrophil chemotaxis that is characterized by selective depletion of a surface protein with a molecular mass of 110 kDa (GP110). Data on partial characterization of GP110 suggest that it is a glycoprotein which is enriched in Lys, Glu, His, Leu, and Ala ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.7.3015-3016.1992

    authors: Tripathi SC,Van Dyke TE

    更新日期:1992-07-01 00:00:00

  • Diversity of the T-cell response to pulmonary Cryptococcus neoformans infection.

    abstract::Cell-mediated immunity plays an important role in immunity to the pathogenic fungus Cryptococcus neoformans. However, the antigen specificity of the T-cell response to C. neoformans remains largely unknown. In this study, we used two approaches to determine the antigen specificity of the T-cell response to C. neoforma...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00080-06

    authors: Lindell DM,Ballinger MN,McDonald RA,Toews GB,Huffnagle GB

    更新日期:2006-08-01 00:00:00

  • Enzymatic degradation of H2O2 by Leptospira.

    abstract::The enzymes responsible for reducing H2O2 were surveyed in 49 strains of Leptospira by using semiquantitative assays for catalase and peroxidase. The survey revealed a differential distribution of catalase and peroxidase activities between the two leptospiral complexes. The pathogenic Leptospira interrogans strains ga...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.22.3.672-675.1978

    authors: Corin RE,Boggs E,Cox CD

    更新日期:1978-12-01 00:00:00

  • Coxiella burnetii superoxide dismutase gene: cloning, sequencing, and expression in Escherichia coli.

    abstract::A superoxide dismutase (SOD) gene from the obligate intracellular bacterium Coxiella burnetii has been cloned, and its DNA sequence has been determined and expressed in Escherichia coli. The gene was identified on pSJR50, a pHC79-derived genomic clone, by using the polymerase chain reaction with degenerate oligonucleo...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.9.3814-3823.1992

    authors: Heinzen RA,Frazier ME,Mallavia LP

    更新日期:1992-09-01 00:00:00

  • Bile acids repress hypoxia-inducible factor 1 signaling and modulate the airway immune response.

    abstract::Gastroesophageal reflux (GER) frequently occurs in patients with respiratory disease and is particularly prevalent in patients with cystic fibrosis. GER is a condition in which the duodenogastric contents of the stomach leak into the esophagus, in many cases resulting in aspiration into the respiratory tract. As such,...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00674-13

    authors: Legendre C,Reen FJ,Woods DF,Mooij MJ,Adams C,O'Gara F

    更新日期:2014-09-01 00:00:00

  • Pseudomonas aeruginosa-mediated damage requires distinct receptors at the apical and basolateral surfaces of the polarized epithelium.

    abstract::Pseudomonas aeruginosa, an important opportunistic pathogen of humans, exploits epithelial damage to establish infection. We have rigorously explored the role of N-glycoproteins and heparan sulfate proteoglycans (HSPGs) in P. aeruginosa-mediated attachment and subsequent downstream events at the apical (AP) and basola...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01215-09

    authors: Bucior I,Mostov K,Engel JN

    更新日期:2010-03-01 00:00:00

  • Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model.

    abstract::Wild-type isolates of Shigella flexneri bind the dye Congo red from solid media, thus producing red (Crb+) colonies. Mutants which fail to bind the dye produce white colonies (Crb-) and are avirulent in a variety of systems. In S. flexneri the ability to bind Congo red correlates with the ability to bind hemin and pro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.6.1393-1398.1987

    authors: Daskaleros PA,Payne SM

    更新日期:1987-06-01 00:00:00

  • Toxicity of recombinant toxic shock syndrome toxin 1 and mutant toxins produced by Staphylococcus aureus in a rabbit infection model of toxic shock syndrome.

    abstract::Menstrually associated toxic shock syndrome (TSS) is attributed primarily to the effects of staphylococcal exotoxin toxic shock syndrome toxin 1 (TSST-1). A region of the 194-amino-acid toxin spanning residues 115 through 144 constitutes a biologically active site. Several point mutations in the TSST-1 gene in that re...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.3.793-799.1993

    authors: Bonventre PF,Heeg H,Cullen C,Lian CJ

    更新日期:1993-03-01 00:00:00

  • X-linked immunodeficient mice as a model for testing the protective efficacy of monoclonal antibodies against Pseudomonas aeruginosa.

    abstract::(DBA/N[female] X CBA/2[male])F1 males have been reported to be deficient in producing antibodies against a number of antigens, including carbohydrates (I. Scher, Adv. Immunol. 35:1-71, 1982). We show that F1 male mice, in contrast to females, made less lipopolysaccharide (LPS)-specific antibodies after immunization wi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.5.1209-1214.1988

    authors: Zweerink HJ,Gammon MC,Hutchison CF,Jackson JJ,Pier GB,Puckett JM,Sewell TJ,Sigal NH

    更新日期:1988-05-01 00:00:00

  • The kinetoplastid membrane protein 11 of Leishmania donovani and African trypanosomes is a potent stimulator of T-lymphocyte proliferation.

    abstract::Kinetoplastid membrane protein 11 (KMP-11) from Leishmania donovani is an abundant 11-kDa surface membrane glycoprotein. Lymph node cells from mice of six different H-2 haplotypes immunized with KMP-11 or with L. donovani promastigotes were stimulated to proliferate in vitro KMP-11. Primed purified T cells required an...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.11.4893-4899.1994

    authors: Tolson DL,Jardim A,Schnur LF,Stebeck C,Tuckey C,Beecroft RP,Teh HS,Olafson RW,Pearson TW

    更新日期:1994-11-01 00:00:00

  • Identification of D motif epitopes in Staphylococcus aureus fibronectin-binding protein for the production of antibody inhibitors of fibronectin binding.

    abstract::A fibronectin-binding protein (FnBP) adhesin of Staphylococcus aureus possesses three 37- or 38-amino-acid motifs (D1, D2, and D3) that can each bind fibronectin (Fn) with low affinity and that in tandem comprise D1-3, a high-affinity Fn-binding domain. To identify epitopes for the generation of adhesion-blocking anti...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.2.537-543.1997

    authors: Sun Q,Smith GM,Zahradka C,McGavin MJ

    更新日期:1997-02-01 00:00:00

  • Pathways for potentiation of immunogenicity during adjuvant-assisted immunizations with Plasmodium falciparum major merozoite surface protein 1.

    abstract::Vaccine adjuvants exert critical and unique influences on the quality of immune responses induced during active immunizations. We investigated the mechanisms of action of immunological adjuvants in terms of their requirements for cytokine-mediated pathways for adjuvanticity. Antibody responses potentiated by several a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.11.5329-5336.1998

    authors: Hui GS,Hashimoto CN

    更新日期:1998-11-01 00:00:00

  • Leishmania pifanoi proteoglycolipid complex P8 induces macrophage cytokine production through Toll-like receptor 4.

    abstract::The P8 proteoglycolipid complex (P8 PGLC) is a glyconjugate expressed by Leishmania mexicana complex parasites. We previously have shown that vaccination with P8 PGLC provides protection against cutaneous leishmaniasis in susceptible BALB/c mice. However, the biological importance of this complex remains unknown. Here...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01528-07

    authors: Whitaker SM,Colmenares M,Pestana KG,McMahon-Pratt D

    更新日期:2008-05-01 00:00:00

  • Contribution of adhesion to bacterial persistence in the mouse urinary tract.

    abstract::The affinity of uropathogenic Escherichia coli to kidneys and bladders of experimentally infected mice was shown to be determined in part by the adhesive properties of the infecting bacteria. Mice were infected with various pairwise combinations of two homogeneic sets of bacteria: (i) mutants derived from a human pyel...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.40.1.265-272.1983

    authors: Hagberg L,Hull R,Hull S,Falkow S,Freter R,Svanborg Edén C

    更新日期:1983-04-01 00:00:00

  • Experimental infection with Chlamydia pneumoniae in nonhuman primates.

    abstract::To serially examine the immunopathogenesis and histopathology of infection with Chlamydia pneumoniae, we inoculated two cynomolgus monkeys in the conjunctival sac, nose, and nasopharynx with C. pneumoniae TWAR. After inoculation, C. pneumoniae was isolated from the inoculation sites and the rectums of both monkeys for...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.3.593-597.1990

    authors: Holland SM,Taylor HR,Gaydos CA,Kappus EW,Quinn TC

    更新日期:1990-03-01 00:00:00

  • Epitope mapping and characterization of antigenic determinants of heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli by using monoclonal antibodies.

    abstract::A panel of monoclonal antibodies (MAbs) specific for the heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli was produced. All four MAbs (8G7, 53-4, 11C, and SH1) bound to native STh in an enzyme-linked immunosorbent assay to various degrees, with clone SH1 showing the best affinity. The MAbs were screen...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.1.289-294.1993

    authors: Takeda T,Nair GB,Suzuki K,Zhe HX,Yokoo Y,De Mol P,Hemelhof W,Butzler JP,Takeda Y,Shimonishi Y

    更新日期:1993-01-01 00:00:00

  • In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity.

    abstract::Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA gene, required for full urease activity, encodes a cytoplasmic membrane nickel transporter that imports nickel ions and le...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.2.685-691.2002

    authors: Nolan KJ,McGee DJ,Mitchell HM,Kolesnikow T,Harro JM,O'Rourke J,Wilson JE,Danon SJ,Moss ND,Mobley HL,Lee A

    更新日期:2002-02-01 00:00:00

  • Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113.

    abstract::Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, eae-positive STEC strains, particularly those belonging to serogroups O157 and O111, appear to have greater virulence for humans. However, in spite of b...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.11.5930-5937.1999

    authors: Paton AW,Paton JC

    更新日期:1999-11-01 00:00:00

  • Interleukin 12 is produced in vivo during endotoxemia and stimulates synthesis of gamma interferon.

    abstract::Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.10.4244-4249.1994

    authors: Heinzel FP,Rerko RM,Ling P,Hakimi J,Schoenhaut DS

    更新日期:1994-10-01 00:00:00

  • Heparin protects human endothelial cells infected by Rickettsia rickettsii.

    abstract::Routine culture of endothelial cells currently includes the use of heparin, which significantly reduces cell doubling time and increases cell population size. Heparin protects cultured arterial endothelial cells from damage by toxic oxygen metabolites produced by the action of xanthine and xanthine oxidase. Because of...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.12.4505-4510.1991

    authors: Silverman DJ,Santucci LA,Sekeyova Z

    更新日期:1991-12-01 00:00:00