Abstract:
:Escherichia coli 536 (O6:K15:H31), which was isolated from a case of urinary tract infection, determines high nephropathogenicity in a rat pyelonephritis system as measured by renal bacterial counts 7 days after infection. The loss of S fimbrial adhesin formation (Sfa-) (mannose-resistant hemagglutination [Mrh-] and fimbria production [Fim-]), serum resistance (Sre-), and hemolysin production (Hly-) in the mutant 536-21 led to a dramatic reduction of bacterial counts from almost 10(5) to only 40 cells per g of kidney. The reintroduction of the cloned S fimbrial adhesin determinant (sfa) increases the virulence of the avirulent mutant strain by a factor of 20; almost the same effect was observed after restoration of serum resistance by integration of an sfa+ recombinant cosmid into the chromosome. Additional reintroduction of the Hly+ phenotype by transformation of two hly determinants increased the virulence of the strains. Hemolysin production determined increased renal elimination of leukocytes and erythrocytes. Thus all three determinants investigated, S fimbriae, serum resistance, and hemolysin, contribute to the multifactorial phenomenon of E. coli nephropathogenicity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Marre R,Hacker J,Henkel W,Goebel Wdoi
10.1128/IAI.54.3.761-767.1986subject
Has Abstractpub_date
1986-12-01 00:00:00pages
761-7issue
3eissn
0019-9567issn
1098-5522journal_volume
54pub_type
杂志文章abstract::Bacterial tyrosine kinases and their cognate protein tyrosine phosphatases are best known for regulating the biosynthesis of polysaccharides. Moreover, their roles in the stress response, DNA metabolism, cell division, and virulence have also been documented. The aim of this study was to investigate the pathogenicity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02713-14
更新日期:2015-02-01 00:00:00
abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1380-1383.1989
更新日期:1989-05-01 00:00:00
abstract::To evaluate the enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides, the rate of isolation of these organisms was compared among individuals with and without diarrhea in Thailand. In two groups of American travelers, A. hydrophila, but not P. shigelloides, was associated with episodes of travelers...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.666-673.1982
更新日期:1982-02-01 00:00:00
abstract::All of the Salmonella enteritidis strains isolated from diseased animals (61 strains) and from beef (2 strains) in Japan and in West Germany (1 strain), except for 2 strains isolated from ducks, harbored either a 36-megadalton (Md) plasmid alone or in combination with several other plasmids of different sizes. It is l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.831-833.1985
更新日期:1985-03-01 00:00:00
abstract::We have developed a novel ileal loop model for use in calves to analyze the contribution of Salmonella enterica serovar Typhimurium type III secretion systems to disease processes in vivo. Our model involves constructing ileal loops with end-to-end anastamoses to restore the patency of the small intestine, thereby all...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7161-7169.2005
更新日期:2005-11-01 00:00:00
abstract::The genes encoding alpha-hemolysin and mannose-resistant hemagglutination were shown to be closely linked in cloned DNA from two Escherichia coli urinary tract isolates of serotypes O4 (J96) and O6 (C1212). DNA hybridization experiments demonstrated that the hly and mrh gene clusters of other E. coli O6 serotypes were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.353-358.1984
更新日期:1984-01-01 00:00:00
abstract::Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, eae-positive STEC strains, particularly those belonging to serogroups O157 and O111, appear to have greater virulence for humans. However, in spite of b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5930-5937.1999
更新日期:1999-11-01 00:00:00
abstract::We have isolated plasma cell hybridomas which secrete monoclonal antibodies directed against Haemophilus ducreyi. Two of these monoclonal antibodies recognize all strains of H. ducreyi tested to date and are capable of detecting the presence of H. ducreyi in skin lesions produced by this pathogen in experimental anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.1.196-198.1984
更新日期:1984-04-01 00:00:00
abstract::Pseudomonas aeruginosa invades various epithelial cell types in vitro and in vivo. The P. aeruginosa genome possesses a gene (flhA) which encodes a protein that is believed to be part of the export apparatus for flagellum assembly and which is homologous to invA of Salmonella spp. Because invA is required for invasion...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.4931-4937.2001
更新日期:2001-08-01 00:00:00
abstract::The etiology of recurrent vulvovaginal candidiasis in otherwise healthy women of child-bearing age remains an enigma. To date, results from both clinical studies and a murine model of vaginal candidiasis indicate that Candida vaginitis can occur in the presence of Candida-specific Th1-type cell-mediated immunity expre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2403-2408.1995
更新日期:1995-07-01 00:00:00
abstract::Shigella flexneri is a gram-negative bacterium that causes bacillary dysentery in humans that is characterized by an acute inflammatory response of the colon. The fate of phagocytes that are infected in vitro with virulent Shigella has been the subject of some investigation and debate. In this study we found that viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.504-513.2005
更新日期:2005-01-01 00:00:00
abstract::Anaplasma phagocytophilum is an obligate intracellular bacterium that infects myeloid cells in the mammalian host. Msp2 (p44) is the major immunodominant outer-membrane protein of these bacteria. We hypothesized that Msp2 acts as an adhesin for A. phagocytophilum entry into granulocytes. This potential role was invest...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4018-4025.2003
更新日期:2003-07-01 00:00:00
abstract::A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3300-3303.2002
更新日期:2002-06-01 00:00:00
abstract::The nonacylated form of protein D (PDm) of Haemophilus influenzae has been shown to induce the production of antibodies that are bactericidal to homologous and heterologous nontypeable H. influenzae (NTHi) strains. In this study, immunization of rats with lipoprotein D (LPD) induced higher levels of anti-protein D imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5010-5016.1997
更新日期:1997-12-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the human nasopharynx, and yet is also an opportunistic pathogen of the upper and lower respiratory tracts. Host microenvironments influence gene expression patterns, likely critical for NTHi persistence. The host sequesters iron as a mechanism ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01227-12
更新日期:2013-04-01 00:00:00
abstract::Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8+ T cells are critical for control of T. cruzi infection, and CD8+ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00275-10
更新日期:2010-07-01 00:00:00
abstract::The Duffy binding protein (DBP) is a vital ligand for Plasmodium vivax blood-stage merozoite invasion, making the molecule an attractive vaccine candidate against vivax malaria. Similar to other blood-stage vaccine candidates, DBP allelic variation eliciting a strain-specific immunity may be a major challenge for deve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05924-11
更新日期:2012-03-01 00:00:00
abstract::Rickettsia rickettsii and R. australis formed infectious antibody complexes when reacted with homologous convalescent serum. The addition of antiglobulin to these complexes showed neutralization of plaques proportional to the concentration of rickettsial immune serum. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.5.966-968.1974
更新日期:1974-05-01 00:00:00
abstract::Streptococcus iniae causes meningoencephalitis and death in commercial fish species and has recently been identified as an emerging human pathogen producing fulminant soft tissue infection. As identified by pulsed-field gel electrophoresis (PFGE), strains causing disease in either fish or humans belong to a single clo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.1994-2000.2001
更新日期:2001-04-01 00:00:00
abstract::Suitability of the rhesus monkey (Macaca mulatta) as an experimental host for evaluation of vaccines against airborne infection with Mycobacterium tuberculosis strain H37Rv was investigated. Nonvaccinated monkeys were exposed to estimated doses of 12, 25, or 49 units of H37Rv in a modified Henderson apparatus, and the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.574-582.1970
更新日期:1970-11-01 00:00:00
abstract::Anopheles gambiae is the major African vector of Plasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00864-06
更新日期:2007-04-01 00:00:00
abstract::Pneumolysin, the cholesterol-dependent cytolysin of Streptococcus pneumoniae, induces inflammatory and apoptotic events in mammalian cells. Toll-like receptor 4 (TLR4) confers resistance to pneumococcal infection via its interaction with pneumolysin, but the underlying mechanisms remain to be identified. In the presen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6479-6487.2005
更新日期:2005-10-01 00:00:00
abstract::The rfb region from Vibrio cholerae O139 strain MO45 was cloned from cosmid gene banks established in Escherichia coli HB101, using an immunoblot assay for screening of the correct clones. Immunoblot analysis of lipopolysaccharide (LPS) preparations revealed the presence of two types of positive clones: (i) those expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3565-3570.1996
更新日期:1996-09-01 00:00:00
abstract::We assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. Paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. This was demons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.2.355-359.1975
更新日期:1975-02-01 00:00:00
abstract::Polymicrobial intra-abdominal infections (IAI) are clinically prevalent and cause significant morbidity and mortality, especially those involving fungi. Our laboratory developed a mouse model of polymicrobial IAI and demonstrated that coinfection with Candida albicans and Staphylococcus aureus (C. albicans/S. aureus) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00348-19
更新日期:2019-07-23 00:00:00
abstract::A gene, dmcA, expressing a methyl-accepting chemotaxis protein (MCP) from the oral spirochete Treponema denticola has been characterized. The gene was initially identified as an open reading frame immediately upstream from the previously characterized prtB protease gene of strain ATCC 35405. Nucleotide sequencing of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4011-4016.1997
更新日期:1997-10-01 00:00:00
abstract::Bacillus cereus causes exotoxin-mediated diarrheal food poisoning. Hemolysin BL (HBL) is a well-characterized B. cereus toxin composed of three components (B, L1, and L2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. Here, we show that HBL causes fluid accumulation in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4423-4428.1995
更新日期:1995-11-01 00:00:00
abstract::Complement-containing immune complexes can be presented to phagocytes by human erythrocytes bearing complement receptor 1 (CR1). Although this has long been assumed to be a mechanism by which humans are able to protect themselves from "extracellular" bacteria such as pneumococci, there is little direct evidence. In th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01263-09
更新日期:2010-07-01 00:00:00
abstract::Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella abortus (BCSP31). Recombinant S. choleraesuis chi 3781 stably maintained the pBA31-R7 plasmid and continued to express t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.610-618.1993
更新日期:1993-02-01 00:00:00
abstract::Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00139-16
更新日期:2016-06-23 00:00:00