Mitotic block and delayed lethality in HeLa epithelial cells exposed to Escherichia coli BM2-1 producing cytotoxic necrotizing factor type 1.


:The cytopathic effect (CPE) of Escherichia coli producing cytotoxic necrotizing factor type 1 (CNF1) was investigated by using a human epithelial cell (HeLa) model of infection with CNF1-producing E. coli BM2-1. This strain was shown to bind loosely, but massively, to HeLa cells. A 4-h interaction between bacteria and eukaryotic cells triggered the delayed appearance of a progressive dose-dependent CPE characterized by (i) intense swelling of cells accompanied by the formation of a dense network of actin stress fibers, (ii) inhibition of cell division due to a complete block in the G2 phase of the cell cycle, and (iii) nucleus swelling and chromatin fragmentation. These alterations resulted in cell death starting about 5 days after interaction. The absence of multinucleation clearly distinguished the CPE from the effect produced by cell-free culture supernatants of infected cells nor prevented by a CNF1-neutralizing antiserum. Pathogenicity was completely abolished after Tn5::phoA insertion mutagenesis in the cnf-1 structural gene but not restored by trans complementation with a recombinant plasmid containing intact cnf-1 and its promoter. These results suggest that a gene downstream of cnf-1, essential to the induction of the CPE, was affected by the mutation. On the other hand, transformation of the wild-type strain BM2-1 with the same recombinant plasmid leads to a significant increase in both CNF1 activity and CPE, demonstrating the direct contribution of CNF1 to the CPE. In conclusion, the pathogenicity of E. coli BM2-1 for HeLa cells results from a complex interaction involving cnf-1 and associated genes and possibly requiring a preliminary step of binding of bacterial organisms to target cells.


Infect Immun


Infection and immunity


De Rycke J,Mazars P,Nougayrede JP,Tasca C,Boury M,Herault F,Valette A,Oswald E




Has Abstract


1996-05-01 00:00:00












  • Role of anti-pilus antibodies in host defense against gonococcal infection studied with monoclonal anti-pilus antibodies.

    abstract::Several monoclonal antibodies directed against gonococcal pili have been used to investigate the potential contribution of anti-pilus antibodies to host defense against gonococcal infection. Included were two antibodies (SM1 and SM2) which reacted with conserved determinants present on pili from all strains tested and...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Virji M,Heckels JE

    更新日期:1985-09-01 00:00:00

  • Restricted replication of Listeria monocytogenes in a gamma interferon-activated murine hepatocyte line.

    abstract::The intracellular pathogen Listeria monocytogenes replicates mainly in resting macrophages and hepatocytes residing in infected tissues. Both innate and acquired resistance strongly depend on activation of listericidal capacities of macrophages by gamma interferon (IFN-gamma) produced by natural killer cells and T lym...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Szalay G,Hess J,Kaufmann SH

    更新日期:1995-08-01 00:00:00

  • Bradyzoite-specific surface antigen SRS9 plays a role in maintaining Toxoplasma gondii persistence in the brain and in host control of parasite replication in the intestine.

    abstract::Toxoplasma gondii is a ubiquitous parasite that persists for the life of a healthy mammalian host. A latent, chronic infection can reactivate upon immunosuppression and cause life-threatening diseases, such as encephalitis. A key to the pathogenesis is the parasite's interconversion between the tachyzoite (in acute in...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kim SK,Karasov A,Boothroyd JC

    更新日期:2007-04-01 00:00:00

  • Experimental elimination of tumor necrosis factor in low-dose endotoxin models has variable effects on survival.

    abstract::Tumor necrosis factor alpha (TNF) is thought to play a major role in the pathogenesis of septic shock. Anti-TNF antibody was preadministered in low-dose endotoxin lethality models in which BALB/c mice were challenged with small amounts of lipopolysaccharide following their sensitization with either carrageenan (CAR) o...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Franks AK,Kujawa KI,Yaffe LJ

    更新日期:1991-08-01 00:00:00

  • Chemotaxigenic activity of extracts from the mycelial and spherule phases of Coccidioides immitis for human polymorphonuclear leukocytes.

    abstract::In order to further characterize human host defenses against Coccidioides immitis, extracts of this fungus were tested in vitro for their ability to attract polymorphonuclear leukocytes derived from peripheral blood of uninfected subjects. Soluble substances prepared from the mycelial (saprophytic) and spherule (tissu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Galgiani JN,Isenberg RA,Stevens DA

    更新日期:1978-09-01 00:00:00

  • Effect of dosage on immunogenicity of a Vi conjugate vaccine injected twice into 2- to 5-year-old Vietnamese children.

    abstract::In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 microg of Vi and induced a greater-than-eightfold rise in immunoglobulin G (IgG) anti-Vi in all of the vaccinees tested. In this investigati...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Canh DG,Lin FY,Thiem VD,Trach DD,Trong ND,Mao ND,Hunt S,Schneerson R,Robbins JB,Chu C,Shiloach J,Bryla DA,Bonnet MC,Schulz D,Szu SC

    更新日期:2004-11-01 00:00:00

  • Recombinant V antigen protects mice against pneumonic and bubonic plague caused by F1-capsule-positive and -negative strains of Yersinia pestis.

    abstract::The purified recombinant V antigen from Yersinia pestis, expressed in Escherichia coli and adsorbed to aluminum hydroxide, an adjuvant approved for human use, was used to immunize outbred Hsd:ND4 mice subcutaneously. Immunization protected mice from lethal bubonic and pneumonic plague caused by CO92, a wild-type F1+ s...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Anderson GW Jr,Leary SE,Williamson ED,Titball RW,Welkos SL,Worsham PL,Friedlander AM

    更新日期:1996-11-01 00:00:00

  • Immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms.

    abstract::Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous ba...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Berg RD,Savage DC

    更新日期:1975-02-01 00:00:00

  • Humoral immune response to plasmid protein pgp3 in patients with Chlamydia trachomatis infection.

    abstract::We identified, by two-dimensional electrophoretic analysis and microsequencing, a protein of Chlamydia trachomatis elementary bodies which corresponds to the polypeptide (pgp3) encoded by open reading frame 3 (ORF3). Amino acid analysis showed that the first residue (Gly) found in the native protein is the one encoded...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Comanducci M,Manetti R,Bini L,Santucci A,Pallini V,Cevenini R,Sueur JM,Orfila J,Ratti G

    更新日期:1994-12-01 00:00:00

  • Coxiella burnetii superoxide dismutase gene: cloning, sequencing, and expression in Escherichia coli.

    abstract::A superoxide dismutase (SOD) gene from the obligate intracellular bacterium Coxiella burnetii has been cloned, and its DNA sequence has been determined and expressed in Escherichia coli. The gene was identified on pSJR50, a pHC79-derived genomic clone, by using the polymerase chain reaction with degenerate oligonucleo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Heinzen RA,Frazier ME,Mallavia LP

    更新日期:1992-09-01 00:00:00

  • Scrapie in vitro: agent replication and reduced cell yield.

    abstract::Exposure of PAM cells, a spontaneously transformed mouse cell line, to brain homogenates from mice infected with scrapie caused a relative decrease in total cell yield, which persisted from passage 2 or 3 to passage 18 after treatment. The effect was elicited by each of the eight independent scrapie isolates tested. L...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Carp RI,Merz GS,Licursi PC

    更新日期:1976-07-01 00:00:00

  • Role of fimbriae in Porphyromonas gingivalis invasion of gingival epithelial cells.

    abstract::Porphyromonas gingivalis is a periodontal pathogen capable of invading primary cultures of normal human gingival epithelial cells (NHGEC). Involvement of P. gingivalis fimbriae in the invasion process was examined. Purified P. gingivalis 33277 fimbriae blocked invasion of this organism into NHGEC in a dose-dependent m...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weinberg A,Belton CM,Park Y,Lamont RJ

    更新日期:1997-01-01 00:00:00

  • Comparison of guinea pig and protozoan models for determining virulence of Legionella species.

    abstract::Legionella pneumophila organisms are able to infect and multiply within the ciliated protozoan Tetrahymena pyriformis. This ability may be associated with virulence, because an attenuated strain of L. pneumophila fails to multiply within this protozoan, whereas a virulent strain increases 10,000-fold in number when co...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fields BS,Barbaree JM,Shotts EB Jr,Feeley JC,Morrill WE,Sanden GN,Dykstra MJ

    更新日期:1986-09-01 00:00:00

  • Microarray-based detection of Salmonella enterica serovar Typhimurium transposon mutants that cannot survive in macrophages and mice.

    abstract::DNA microarrays provide an opportunity to combine the principles of signature-tagged mutagenesis (STM) with microarray technology to identify potentially important bacterial virulence genes. The scope of DNA microarrays allows for less laborious screening on a much larger scale than possible by STM alone. We have adap...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chan K,Kim CC,Falkow S

    更新日期:2005-09-01 00:00:00

  • Stimulation of immunoglobulin-containing cells and isotype-specific antibody response in experimental Mycoplasma hyopneumoniae infection in specific-pathogen-free pigs.

    abstract::The development of immunoglobulin-containing cells and antigen-specific plasma cells in retropharyngeal and bronchial lymph nodes, in lung tissue, and in nasal mucous membrane was followed during an experimental infection of pigs with Mycoplasma hyopneumoniae. An enzyme-linked immunosorbent assay was used to follow st...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Suter M,Kobisch M,Nicolet J

    更新日期:1985-09-01 00:00:00

  • The cell wall mediates pneumococcal attachment to and cytopathology in human endothelial cells.

    abstract::Streptococcus pneumoniae interacts with vascular endothelial cells during the course of bacteremia. In this study, we characterized the initial attachment of pneumococci to human endothelial cells (EC) and the response of the endothelium to this interaction. Pneumococci adhered to EC in a dose-dependent fashion. Attac...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Geelen S,Bhattacharyya C,Tuomanen E

    更新日期:1993-04-01 00:00:00

  • Experimental Fasciola hepatica infection alters responses to tests used for diagnosis of bovine tuberculosis.

    abstract::Fasciola hepatica is a prevalent helminth parasite of livestock. Infection results in polarization of the host's immune response and generation of type 2 helper (Th2) immune responses, which are known to be inhibitory to Th1 responses. Bovine tuberculosis (BTB) is a bacterial disease of economic and zoonotic importanc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Flynn RJ,Mannion C,Golden O,Hacariz O,Mulcahy G

    更新日期:2007-03-01 00:00:00

  • Purification and analysis with monoclonal antibodies of P2, the major outer membrane protein of nontypable Haemophilus influenzae.

    abstract::The protein P2 comprises a large proportion of the outer membrane of nontypable Haemophilus influenzae and functions as a porin. In view of the importance of the protein as a surface antigen, the present study was designed to purify and analyze P2 with particular emphasis on detection of antigenic determinants express...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Murphy TF,Bartos LC

    更新日期:1988-05-01 00:00:00

  • Regulation of mycobacterial growth by the hypothalamus-pituitary-adrenal axis: differential responses of Mycobacterium bovis BCG-resistant and -susceptible mice.

    abstract::The role of the hypothalamus-pituitary-adrenal (HPA) axis in regulating the growth of Mycobacterium avium in Mycobacterium bovis BCG-resistant and -susceptible congenic mice was evaluated. Restraint was used to activate the HPA axis, which resulted in an increase in the level of corticosterone in the plasma. Activatio...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Brown DH,Sheridan J,Pearl D,Zwilling BS

    更新日期:1993-11-01 00:00:00

  • Designing therapies against experimental visceral leishmaniasis by modulating the membrane fluidity of antigen-presenting cells.

    abstract::The membrane fluidity of antigen-presenting cells (APCs) has a significant bearing on T-cell-stimulating ability and is dependent on the cholesterol content of the membrane. The relationship, if any, between membrane fluidity and defective cell-mediated immunity in visceral leishmaniasis has been investigated. Systemi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Banerjee S,Ghosh J,Sen S,Guha R,Dhar R,Ghosh M,Datta S,Raychaudhury B,Naskar K,Haldar AK,Lal CS,Pandey K,Das VN,Das P,Roy S

    更新日期:2009-06-01 00:00:00

  • Role of endogenous interleukin-12 in immune response to staphylococcal enterotoxin B in mice.

    abstract::In the present study, the roles of interleukin 12 (IL-12) and IL-18 and their possible interaction during superantigen-induced responses were studied by injection of staphylococcal enterotoxin B (SEB) into mice. These data suggest that the role of IL-12 in SEB-induced responses is limited to sustaining gamma interfero...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lauw FN,Florquin S,Speelman P,van Deventer SJ,van der Poll T

    更新日期:2001-09-01 00:00:00

  • The cellular source of interleukin-6 during Listeria infection.

    abstract::The cellular source of interleukin-6 (IL-6) during infection of mice with Listeria monocytogenes was investigated both in vitro and in vivo. Peritoneal cells taken at intervals from infected mice and cultured in vitro without added stimulus produced high titers of IL-6 peaking 2 days postinfection in a time course sim...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Liu Z,Cheers C

    更新日期:1993-06-01 00:00:00

  • Partial characterization of an external polysaccharide of Helicobacter pylori by using an immunoglobulin M monoclonal antibody.

    abstract::A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant wa...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Drouet EB,De Montclos HP,Andujar M,Boude M,Grimaud JA,Denoyel GA

    更新日期:1993-06-01 00:00:00

  • Circulating CD4 and CD8 T cells have little impact on host defense against experimental vaginal candidiasis.

    abstract::The etiology of recurrent vulvovaginal candidiasis in otherwise healthy women of child-bearing age remains an enigma. To date, results from both clinical studies and a murine model of vaginal candidiasis indicate that Candida vaginitis can occur in the presence of Candida-specific Th1-type cell-mediated immunity expre...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fidel PL Jr,Lynch ME,Sobel JD

    更新日期:1995-07-01 00:00:00

  • Solubilization and partial characterization of the intestinal receptor for Escherichia coli heat-stable enterotoxin.

    abstract::Binding of Escherichia coli strain 431 heat-stable enterotoxin (STa) and activation of intestinal particulate guanylate cyclase by E. coli STa were studied with rat intestinal epithelial cells and brush border membranes (BBMs). The rates of guanylate cyclase stimulation by 431 STa in cells and BBMs were rapid, with ma...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Dreyfus LA,Robertson DC

    更新日期:1984-11-01 00:00:00

  • Early gamma interferon production by natural killer cells is important in defense against murine listeriosis.

    abstract::A spot enzyme-linked immunosorbent assay was used to show that subcutaneous inoculation of a sublethal number of Listeria monocytogenes resulted in the early appearance of gamma interferon (IFN-gamma)-producing cells in the draining lymph nodes. In contrast, inoculation of UV-killed L. monocytogenes failed to cause th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Dunn PL,North RJ

    更新日期:1991-09-01 00:00:00

  • Neither motility nor chemotaxis plays a role in the ability of Escherichia coli F-18 to colonize the streptomycin-treated mouse large intestine.

    abstract::Escherichia coli F-18, isolated from the feces of a healthy human in 1977, is an excellent colonizer of the streptomycin-treated mouse large intestine and displays normal motility and chemotaxis ability. A chemotaxis-defective derivative of E. coli F-18, E, coli F-18 CheA-, and a nonflagellated derivative, E. coli F-1...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McCormick BA,Laux DC,Cohen PS

    更新日期:1990-09-01 00:00:00

  • Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli.

    abstract::The selC tRNA gene is a common site for the insertion of pathogenicity islands in a variety of bacterial enteric pathogens. We demonstrate here that Escherichia coli that produces Shiga toxin 2d and does not harbor the locus of enterocyte effacement (LEE) contains, instead, a novel genomic island. In one representativ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Schmidt H,Zhang WL,Hemmrich U,Jelacic S,Brunder W,Tarr PI,Dobrindt U,Hacker J,Karch H

    更新日期:2001-11-01 00:00:00

  • Cell-associated hemagglutinin-deficient mutant of Vibrio cholerae.

    abstract::Cell-associated hemagglutinin-negative mutants were derived from cholera enterotoxin-negative Vibrio cholerae JBK70 by Tn5 mutagenesis. One of the mutants identified, SB001, was characterized in greater detail. Its ability to colonize ilea of adult rabbits was determined by feeding approximately 10(8) V. cholerae to e...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Finn TM,Reiser J,Germanier R,Cryz SJ Jr

    更新日期:1987-04-01 00:00:00

  • Increased virulence of a fibronectin-binding protein mutant of Staphylococcus aureus in a rat model of pneumonia.

    abstract::Fibronectin-binding proteins mediate Staphylococcus aureus internalization into nonphagocytic cells in vitro. We have investigated whether fibronectin-binding proteins are virulence factors in the pathogenesis of pneumonia by using S. aureus strain 8325-4 and isogenic mutants in which fibronectin-binding proteins were...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McElroy MC,Cain DJ,Tyrrell C,Foster TJ,Haslett C

    更新日期:2002-07-01 00:00:00