Mitotic block and delayed lethality in HeLa epithelial cells exposed to Escherichia coli BM2-1 producing cytotoxic necrotizing factor type 1.


:The cytopathic effect (CPE) of Escherichia coli producing cytotoxic necrotizing factor type 1 (CNF1) was investigated by using a human epithelial cell (HeLa) model of infection with CNF1-producing E. coli BM2-1. This strain was shown to bind loosely, but massively, to HeLa cells. A 4-h interaction between bacteria and eukaryotic cells triggered the delayed appearance of a progressive dose-dependent CPE characterized by (i) intense swelling of cells accompanied by the formation of a dense network of actin stress fibers, (ii) inhibition of cell division due to a complete block in the G2 phase of the cell cycle, and (iii) nucleus swelling and chromatin fragmentation. These alterations resulted in cell death starting about 5 days after interaction. The absence of multinucleation clearly distinguished the CPE from the effect produced by cell-free culture supernatants of infected cells nor prevented by a CNF1-neutralizing antiserum. Pathogenicity was completely abolished after Tn5::phoA insertion mutagenesis in the cnf-1 structural gene but not restored by trans complementation with a recombinant plasmid containing intact cnf-1 and its promoter. These results suggest that a gene downstream of cnf-1, essential to the induction of the CPE, was affected by the mutation. On the other hand, transformation of the wild-type strain BM2-1 with the same recombinant plasmid leads to a significant increase in both CNF1 activity and CPE, demonstrating the direct contribution of CNF1 to the CPE. In conclusion, the pathogenicity of E. coli BM2-1 for HeLa cells results from a complex interaction involving cnf-1 and associated genes and possibly requiring a preliminary step of binding of bacterial organisms to target cells.


Infect Immun


Infection and immunity


De Rycke J,Mazars P,Nougayrede JP,Tasca C,Boury M,Herault F,Valette A,Oswald E




Has Abstract


1996-05-01 00:00:00












  • Antibody responses in adult volunteers to pneumococcal polysaccharide types 19F and 19A administered singly and in combination.

    abstract::The immunogenicity of the two major group 19 pneumococcal polysaccharides, types 19F and 19A, was studied in human volunteers. Antibody responses after vaccination with either 50 micrograms of type 19F polysaccharide, 50 micrograms of type 19A polysaccharide, or 25 micrograms of both polysaccharides premixed in a sing...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章


    authors: Penn RL,Lewin EB,Douglas RG Jr,Schiffman G,Lee CJ,Robbins JB

    更新日期:1982-06-01 00:00:00

  • Growth control of small-colony variants by genetic regulation of the hemin uptake system.

    abstract::Small-colony variants (SCVs) are slow-growing variants of human bacterial pathogens. They are associated with chronic persistent infections, and their biochemical identification and antimicrobial treatment are impaired by altered metabolic properties. To contribute to the understanding of SCV-mediated infections, we a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Roggenkamp A,Hoffmann H,Hornef MW

    更新日期:2004-04-01 00:00:00

  • Immunization with genetic toxoids of the Arcanobacterium pyogenes cholesterol-dependent cytolysin, pyolysin, protects mice against infection.

    abstract::Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F(497), HIS-PLO.Delta P(499), and HI...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jost BH,Trinh HT,Songer JG,Billington SJ

    更新日期:2003-05-01 00:00:00

  • Haemophilus ducreyi targets Src family protein tyrosine kinases to inhibit phagocytic signaling.

    abstract::Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, has been shown to inhibit phagocytosis of both itself and secondary targets in vitro. Immunodepletion of LspA proteins from H. ducreyi culture supernatant fluid abolished this inhibitory effect, indicating that the LspA proteins ar...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mock JR,Vakevainen M,Deng K,Latimer JL,Young JA,van Oers NS,Greenberg S,Hansen EJ

    更新日期:2005-12-01 00:00:00

  • Role of Escherichia coli alpha-hemolysin and bacterial adherence in infection: requirement for release of inflammatory mediators from granulocytes and mast cells.

    abstract::We investigated the role of bacterial mannose-resistant fimbriation of S fimbriae (Fim), mannose-resistant hemagglutination (S-Mrh), and hemolysin (Hly) production by an Escherichia coli parent and genetically cloned strains as regards their effect on histamine release from rat mast cells and generation of the chemilu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: König B,König W,Scheffer J,Hacker J,Goebel W

    更新日期:1986-12-01 00:00:00

  • Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces.

    abstract::Cells of several strains of Streptococcus gordonii attached in much higher numbers to experimental pellicles formed from samples of submandibular or parotid saliva on hydroxyapatite (HA) beads than to buffer controls. The nature of the salivary components responsible were investigated by preparing experimental pellicl...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gibbons RJ,Hay DI,Schlesinger DH

    更新日期:1991-09-01 00:00:00

  • Spectrum of Legionella species whose intracellular multiplication in murine macrophages is genetically controlled by Lgn1.

    abstract::We examined the intracellular growth of 20 strains within six species of Legionella in thioglycolate-elicited peritoneal macrophages from A/J and C57BL/6 mice and a congenic strain derived from them (A.B Lgn1). With the exception of Legionella pneumophila Togus-1 and Bloomington-2, the intracellular growth of the 15 L...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Miyamoto H,Maruta K,Ogawa M,Beckers MC,Gros P,Yoshida S

    更新日期:1996-05-01 00:00:00

  • Role of receptor binding in toxicity, immunogenicity, and adjuvanticity of Escherichia coli heat-labile enterotoxin.

    abstract::The role of receptor binding in the toxicity, immunogenicity, and adjuvanticity of the heat-labile enterotoxin of Escherichia coli (LT) was examined by comparing native LT and LT(G33D), a B-subunit receptor binding mutant, with respect to the ability to bind to galactose and to GM1, toxicity on mouse Y-1 adrenal tumor...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Guidry JJ,Cárdenas L,Cheng E,Clements JD

    更新日期:1997-12-01 00:00:00

  • Cold-adapted variants of influenza A virus: evaluation in adult seronegative volunteers of A/Scotland/840/74 and A/Victoria/3/75 cold-adapted recombinants derived from the cold-adapted A/Ann Arbor/6/60 strain.

    abstract::Influenza A/Scotland/74 (H3N2) and A/Victoria/75 (H3N2) cold-adapted (ca) recombinant viruses, prepared by mating the A/Ann Arbor/6/60 (H2N2) ca donor virus and influenza A wild-type virus, were evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer,

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Murphy BR,Holley HP Jr,Berquist EJ,Levine MM,Spring SB,Maassab HF,Kendal AP,Chanock RM

    更新日期:1979-02-01 00:00:00

  • Risk factors in the pathogenesis of invasive group A streptococcal infections: role of protective humoral immunity.

    abstract::An impressive change in the epidemiology and severity of invasive group A streptococcal infections occurred in the 1980s, and the incidence of streptococcal toxic shock syndrome cases continues to rise. The reason for the resurgence of severe invasive cases remains a mystery-has there been a change in the pathogen or ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Basma H,Norrby-Teglund A,Guedez Y,McGeer A,Low DE,El-Ahmedy O,Schwartz B,Kotb M

    更新日期:1999-04-01 00:00:00

  • Separation of Sendai virus glycoproteins by using glutaraldehyde-treated erythrocytes and preparation of monospecific antisera against the glycoproteins.

    abstract::Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins, F and HN, were separated from Triton X-100- or Nonidet P-40-solubilized envelopes as unadsorbed and eluted fractions, respectively, by using glutaralde-hyde-treated chicken erythrocytes. These separated glycoproteins were biologically active. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hosaka Y

    更新日期:1980-10-01 00:00:00

  • Passive hemolysis test for antibody to Treponema hyodysenteriae.

    abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jenkins EM,Sinha PP,Vance RT,Reese GL

    更新日期:1976-10-01 00:00:00

  • Haemophilus somnus induces apoptosis in bovine endothelial cells in vitro.

    abstract::Haemophilus somnus causes pneumonia, reproductive failure, infectious myocarditis, thrombotic meningoencephalitis, and other diseases in cattle. Although vasculitis is commonly seen as a result of systemic H. somnus infections, the pathogenesis of vascular damage is poorly characterized. In this study, we demonstrated...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sylte MJ,Corbeil LB,Inzana TJ,Czuprynski CJ

    更新日期:2001-03-01 00:00:00

  • Immunoglobulin and complement in tissues of mice infected with Nocardia brasiliensis.

    abstract::Two weeks after mice had been infected in the footpad with cells of Nocardia brasiliensis, immunoglobulin and C3 deposits were observed in tissue from the inoculation site. Both immunoglobulin and C3 were found in the actinomycotic granules that characterize the mycetoma and in the surrounding inflammatory zones, in w...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Conde C,Mancilla R,Fresan M,Ortiz-Ortiz L

    更新日期:1983-06-01 00:00:00

  • Borrelia burgdorferi RNA induces type I and III interferons via Toll-like receptor 7 and contributes to production of NF-κB-dependent cytokines.

    abstract::Borrelia burgdorferi elicits a potent cytokine response through activation of multiple signaling receptors on innate immune cells. Spirochetal lipoproteins initiate expression of NF-κB-dependent cytokines primarily via TLR2, whereas type I interferon (IFN) production is induced through the endosomal receptors TLR7 and...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Love AC,Schwartz I,Petzke MM

    更新日期:2014-06-01 00:00:00

  • Secreted Phosphatase and Deoxyribonuclease Are Required by Pseudomonas aeruginosa To Defend against Neutrophil Extracellular Traps.

    abstract::Neutrophil extracellular traps (NETs) are produced by neutrophils as an innate immune defense mechanism to trap and kill microbial pathogens. NETs are comprised of ejected chromatin that forms a lattice structure enmeshed with numerous antimicrobial proteins. In addition to forming the structural backbone of NETs, ext...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wilton M,Halverson TWR,Charron-Mazenod L,Parkins MD,Lewenza S

    更新日期:2018-08-22 00:00:00

  • Sigma B contributes to Listeria monocytogenes gastrointestinal infection but not to systemic spread in the guinea pig infection model.

    abstract::Contributions of the alternative sigma factor sigmaB to Listeria monocytogenes infection were investigated using strains bearing null mutations in sigB, prfA, or inlA or in selected inlA or prfA promoter regions. The DeltaP4inlA strain, which has a deletion in the sigmaB-dependent P4inlA promoter, and the DeltasigB st...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Garner MR,Njaa BL,Wiedmann M,Boor KJ

    更新日期:2006-02-01 00:00:00

  • Morphological and immunohistochemical studies of the lungs and bronchus-associated lymphoid tissue in a rat model of chronic pulmonary infection with Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is one of the most frequently encountered bacterial pathogens in patients with chronic pulmonary infections, including cystic fibrosis and diffuse panbronchiolitis. Bronchus-associated lymphoid tissue (BALT), noted frequently in patients with cystic fibrosis and diffuse panbronchiolitis, is cons...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Iwata M,Sato A

    更新日期:1991-04-01 00:00:00

  • Monoclonal antibodies against Vibrio vulnificus RtxA1 elicit protective immunity through distinct mechanisms.

    abstract::Vibrio vulnificus causes rapidly progressing septicemia with an extremely high mortality rate (≥50%), even with aggressive antibiotic treatment. The bacteria secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins, which are involved in the pathogenesis of Gram-negative Vibrio species. Recently, we repo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lee TH,Cha SS,Lee CS,Rhee JH,Chung KM

    更新日期:2014-11-01 00:00:00

  • Comparison of plasmid deoxyribonucleic acid contents, culture characteristics, and indices of pathogenicity among selected strains of Vibrio parahaemolyticus.

    abstract::Thirty-one Kanagawa-positive and -negative Vibrio parahaemolyticus isolates from patient stools and seafood were examined for plasmid deoxyribonucleic acid content, culture characteristics, and indices of pathogenicity. No significant correlation was found between plasmid deoxyribonucleic acid contents and indices of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Twedt RM,Brown DF,Zink DL

    更新日期:1981-07-01 00:00:00

  • Roles of OspA, OspB, and flagellin in protective immunity to Lyme borreliosis in laboratory mice.

    abstract::Vaccination with recombinant outer surface protein A (OspA) has been shown to protect mice from infection with Borrelia burgdorferi, the Lyme disease agent. To determine whether antibodies to B. burgdorferi proteins other than OspA are involved in protective immunity, antibodies to OspA were removed from protective an...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fikrig E,Barthold SW,Marcantonio N,Deponte K,Kantor FS,Flavell RA

    更新日期:1992-02-01 00:00:00

  • Immunogenicity in a mouse model of a conjugate vaccine made with a synthetic single repeating unit of type 14 pneumococcal polysaccharide coupled to CRM197.

    abstract::Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide frag...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mawas F,Niggemann J,Jones C,Corbel MJ,Kamerling JP,Vliegenthart JF

    更新日期:2002-09-01 00:00:00

  • Synthesis of a conjugate vaccine composed of pneumococcus type 14 capsular polysaccharide bound to pertussis toxin.

    abstract::Type 14 is one of the common types isolated from patients of all ages with infections caused by Streptococcus pneumoniae. Its capsular polysaccharide (Pn14) is composed of a neutrally charged tetrasaccharide repeat unit. Pn14 does not elicit protective levels of antibodies in infants and children and is a less than op...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Schneerson R,Levi L,Robbins JB,Bryla DM,Schiffman G,Lagergard T

    更新日期:1992-09-01 00:00:00

  • 125I-labeled peptide mapping of some heat-modifiable proteins of the gonococcal outer membrane.

    abstract::Gonococci from opaque colonies have cell wall outer membrane proteins that are lacking from organisms which form transparent colonies. These "colony opacity-associated" proteins are among a group of "minor" proteins that exhibit heat modification of their apparent subunit molecular sizes, are easily extracted by deoxy...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Swanson J

    更新日期:1980-04-01 00:00:00

  • Humoral immunity in leprosy: immunoglobulin G and M antibody responses to Mycobacterium leprae in relation to various disease patterns.

    abstract::A solid-phase radioimmunoassay, applying whole Mycobacterium leprae as antigen and radiolabeled protein A from Staphylococcus aureus as antibody-detecting reagent, was used for the determination of specific immunoglobulin G (IgG) and IgM antibody responses in leprosy patients. High IgG anti-M. leprae antibody levels w...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Touw J,Langendijk EM,Stoner GL,Belehu A

    更新日期:1982-06-01 00:00:00

  • Aromatic compound-dependent Brucella suis is attenuated in both cultured cells and mouse models.

    abstract::The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Foulongne V,Walravens K,Bourg G,Boschiroli ML,Godfroid J,Ramuz M,O'Callaghan D

    更新日期:2001-01-01 00:00:00

  • p38 mitogen-activated protein kinase controls NF-kappaB transcriptional activation and tumor necrosis factor alpha production through RelA phosphorylation mediated by mitogen- and stress-activated protein kinase 1 in response to Borrelia burgdorferi antig

    abstract::The interaction of Borrelia burgdorferi, the causative agent of Lyme borreliosis, with phagocytic cells induces the activation of NF-kappaB and the expression of proinflammatory cytokines including tumor necrosis factor alpha (TNF-alpha). B. burgdorferi-induced TNF-alpha production is also dependent on the activation ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Olson CM,Hedrick MN,Izadi H,Bates TC,Olivera ER,Anguita J

    更新日期:2007-01-01 00:00:00

  • Infection with Mycobacterium avium differentially regulates the expression of iron transport protein mRNA in murine peritoneal macrophages.

    abstract::Iron is an important element for the growth of microorganisms as well as in the defense of the host by serving as a catalyst for the generation of free radicals via the Fenton/Haber-Weiss reactions. The iron transporter natural resistance-associated macrophage protein 1 (Nramp1) confers resistance to the growth of a v...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zhong W,Lafuse WP,Zwilling BS

    更新日期:2001-11-01 00:00:00

  • Immune selection for antigenic drift of major outer membrane protein P2 of Haemophilus influenzae during persistence in subcutaneous tissue cages in rabbits.

    abstract::During persistence of nonencapsulated Haemophilus influenzae in the respiratory tracts of patients with chronic bronchitis, the major outer membrane proteins (MOMPs) P2 and P5 show antigenic drift. The hypothesis that appearance of antigenic variants is the consequence of antibody-dependent selection was tested in a r...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Vogel L,Duim B,Geluk F,Eijk P,Jansen H,Dankert J,vanAlphen L

    更新日期:1996-03-01 00:00:00

  • Association of fibril structure formation with cell surface properties of Yersinia enterocolitica.

    abstract::Electron microscopic examination of virulence plasmid-bearing cells of Yersinia enterocolitica revealed the formation of a fibril structure when grown at 37 degrees C but not at 22 degrees C. Plasmidless cells did not exhibit any surface matrix. The formation of this surface component was associated with increased cel...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lachica RV,Zink DL,Ferris WR

    更新日期:1984-10-01 00:00:00