Solid-phase radioimmunoassay for the detection of rotavirus.

Abstract:

:A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sensitive for detection of the virus in stool and up to 128 times as sensitive for detection of a purified virus antigen. In a field study on stool specimens it was at least as efficient as electron microscopy.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Middleton PJ,Holdaway MD,Petric M,Szymanski MT,Tam JS

doi

10.1128/IAI.16.2.439-444.1977

subject

Has Abstract

pub_date

1977-05-01 00:00:00

pages

439-44

issue

2

eissn

0019-9567

issn

1098-5522

journal_volume

16

pub_type

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