Abstract:
:The susceptibility of HeLa cells to Mycoplasma pneumoniae-induced injury was examined. Infections were initiated with relatively low mycoplasma doses, carried out in a culture medium incapable of supporting M. pneumoniae replication in the absence of host cells, and monitored for up to 10 days. Under these conditions, a time- and dose-dependent decline in the number of viable host cells compared with that of uninfected controls was observed. The effect of M. pneumoniae infection on host cell macromolecular synthesis was also evaluated. At high doses of infection, synthesis of both protein and RNA declined rapidly relative to that in control cells. At lower doses there was a biphasic response in protein synthesis, which was substantially lower than that in the uninfected control by day 1 postinfection, returned to control levels by day 4 postinfection, and was again less than that in control cells by day 7 postinfection. In contrast, no transient recovery was observed in RNA synthesis, which declined very gradually over 7 days in infected HeLa cells compared with that in uninfected control cells. The ability of HeLa cells to support the proliferation of M. pneumoniae under these experimental conditions was demonstrated by quantitation of mycoplasma CFU in the nonpermissive medium in the presence or absence of HeLa cells. A negligible increase in the number of M. pneumoniae was observed over 4 days when HeLa cells were absent, while CFU increased by almost 20-fold when M. pneumoniae was cultured in the presence of HeLa cells. The susceptibility and response in macromolecular synthesis in M. pneumoniae-infected HeLa cells differed from that recently described for a nontransformed culture of hamster trachea epithelial cells under the same experimental conditions (Y.-Y. Chen and D.C. Krause, Infect. Immun. 56: 570-576, 1988), underscoring the importance of the choice of host cell for in vitro modeling of M. pneumoniae pathogenesis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Krause DC,Chen YYdoi
10.1128/IAI.56.8.2054-2059.1988subject
Has Abstractpub_date
1988-08-01 00:00:00pages
2054-9issue
8eissn
0019-9567issn
1098-5522journal_volume
56pub_type
杂志文章abstract::Mapping vectors containing antibiotic resistance markers inserted adjacent to or within different cloned genes from Streptococcus mutans were used to determine the relative positions of these genes on the chromosome. The gtfA, ftf, and scrB genes were inserted into streptococcal mapping vector pVA891 adjacent to an Em...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.805-809.1989
更新日期:1989-03-01 00:00:00
abstract::We have developed an animal model for studying mycobacterial pathogenesis using Mycobacterium marinum and the goldfish, Carassius auratus. Goldfish are injected intraperitoneally with doses between 10(2) and 10(9) CFU of M. marinum organisms. Depending on the dose of M. marinum organisms administered, an acute or chro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2938-2942.1998
更新日期:1998-06-01 00:00:00
abstract::Changes in periodontal status are associated with shifts in the composition of the bacterial community in the periodontal pocket. The relative abundances of several newly recognized microbial species, including Filifactor alocis, as-yet-unculturable organisms, and other fastidious organisms have raised questions on th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01727-14
更新日期:2014-08-01 00:00:00
abstract::The presence of lipopolysaccharide (LPS) in gram-negative bacterial repeats-in-toxin (RTX) toxin preparations, as well as the harsh conditions required to remove it, suggests that LPS may complex with RTX toxins. Concentrated culture supernatant (CCS) preparations of the RTX toxin Pasteurella haemolytica leukotoxin (L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2920-2927.1999
更新日期:1999-06-01 00:00:00
abstract::Several strains of group B Streptococcus agalactiae were found to be lethal for young adult rats. When bacteria were heat killed and then injected intraperitoneally into rats, rapid death (14 to 18 h) of the rats occurred, characterized by labored breathing, hemolyzed serum, hemoglobinuria, and subungual hemorrhages. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.2.560-564.1985
更新日期:1985-05-01 00:00:00
abstract::The infiltration of neutrophils which phagocytose and kill microorganisms is an important defense mechanism against infections of the airways. Bordetella pertussis is a human respiratory pathogen which colonizes ciliated epithelium, causing whooping cough. We have investigated the effects of the peptidoglycan fragment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.639-643.1994
更新日期:1994-02-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) infection of beige (bg/bg) mice induced levels of natural killer cytolytic activity comparable to that of uninfected normal bg/+ controls, but considerably less than natural killer activity in VSV-infected bg/+ mice. In contrast, VSV-infected bg/bg and bg/+ mice had essentially equival...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.204-210.1981
更新日期:1981-04-01 00:00:00
abstract::When inoculated orally, Escherichia coli strain 1056 caused acute enteritis in 7 of 22 weanling hamsters. E. coli strain 1056 was isolated from the ileum of a hamster with proliferative ileitis. It was lactose negative, nonmotile, and anaerogenic. By electron microscopy and indirect fluorescent-antibody techniques, E....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1232-1238.1981
更新日期:1981-03-01 00:00:00
abstract::Immunoglobulin A1 (IgA1) proteases are produced by a number of different species of bacteria which cause infection at human mucosal surfaces. The sole substrate of these proteases is human IgA1. Cleavage is within the hinge region of IgA1, although there is variability in the exact peptide bond within the hinge region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.320-331.1990
更新日期:1990-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.243-248.1971
更新日期:1971-02-01 00:00:00
abstract::Encapsulated cells of Cryptococcus neoformans are potent activators of the alternative complement pathway. Previous studies found that monoclonal antibodies (MAbs) specific for the major capsular polysaccharide, termed glucuronoxylomannan (GXM), can markedly suppress the ability of the capsule to accumulate C3 from no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1547-1553.1998
更新日期:1998-04-01 00:00:00
abstract::Hookworm glutathione S-transferases (GSTs) are critical for parasite blood feeding and survival and represent potential targets for vaccination. Three cDNAs, each encoding a full-length GST protein from the human hookworm Necator americanus (and designated Na-GST-1, Na-GST-2, and Na-GST-3, respectively) were isolated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00848-09
更新日期:2010-04-01 00:00:00
abstract::The orotidine-5'-monophosphate decarboxylase (OMPDC) gene, encoding the final enzyme of the de novo pyrimidine biosynthesis pathway, was deleted using Toxoplasma gondii KU80 knockouts to develop an avirulent nonreverting pyrimidine auxotroph strain. Additionally, to functionally address the role of the pyrimidine salv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00287-10
更新日期:2010-09-01 00:00:00
abstract::We demonstrate that a mucoid, alginate-producing strain of Pseudomonas aeruginosa isolated from the lungs of a cystic fibrosis (CF) patient secretes multiple enzymes with nucleoside diphosphate kinase (Ndk), ATPase, adenylate kinase, 5'-nucleotidase, and ATP-modifying enzymatic activities. The secretion is triggered a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5231-5242.1999
更新日期:1999-10-01 00:00:00
abstract::A canine distemper virus (CDV), DESIGNATED R252, originally recovered from a dog with demyelinating encephalomyelitis was shown to reproduce this disease in gnotobiotic dogs with a high incidence as compared to other CDV strains, which produced an acute fatal infection. In this investigation, R252 was propagated for t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.835-844.1975
更新日期:1975-04-01 00:00:00
abstract::The gene encoding the 35-kDa immunogenic Treponema pallidium subsp. pallidum (T. pallidum) membrane protein C, TmpC, was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence carries on N-terminal signal sequence with a four-amino-acid motif, which is characteristic for bacterial lipopr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3536-3546.1991
更新日期:1991-10-01 00:00:00
abstract::We studied seven strains of group G streptococci isolated from clinically severe bacteremic infections in six intravenous drug abusers. These group G strains multiplied luxuriantly in fresh human blood. On electron microscopy, they exhibited surface fibrillae similar to those observed in M-protein-rich group A strepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.753-757.1987
更新日期:1987-03-01 00:00:00
abstract::Cell-mediated immunity to herpes simplex virus type 1 (HSV-1) was assessed by a lymphocytotoxicity 51-Cr-release microassay procedure, using the MA-160 human prostatic adenoma cell line chronically infected with HSV-1 and its parent cell line as control. The specific immune release mean plus or minus standard deviatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.1.76-80.1975
更新日期:1975-07-01 00:00:00
abstract::Gamma interferon (IFN-γ) drives antiparasite responses and immunopathology during infection with Plasmodium species. Immunity-related GTPases (IRGs) are a class of IFN-γ-dependent proteins that are essential for cell autonomous immunity to numerous intracellular pathogens. However, it is currently unknown whether IRGs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02701-14
更新日期:2015-04-01 00:00:00
abstract::Hemin induced germination of Candida albicans blastoconidia when cells grown up to the early exponential phase were shifted from 28 to 37 degrees C (70 to 75% of cells exhibited germ tubes). N-Acetyl-D-glucosamine (GlcNAc), another inducer of myceliation in this fungus, caused a similar effect. The combination of hemi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4360-4364.1997
更新日期:1997-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1352-1358.2002
更新日期:2002-03-01 00:00:00
abstract::In bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4005-4014.1994
更新日期:1994-09-01 00:00:00
abstract::Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.751-755.1988
更新日期:1988-04-01 00:00:00
abstract::Classical and El Tor strains of Vibrio cholerae were examined for production of bacteriophage or bacteriophage-related material by electron microscopy of mitomycin C-induced cultures. The strains were also tested for pathogenicity by using the ligated ileal loops of adult rabbits. Of the 27 strains tested, 25 showed a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.1.4.417-420.1970
更新日期:1970-04-01 00:00:00
abstract::Infection with Mycobacterium avium subsp. paratuberculosis is associated with high levels of morbidity, decreased production, and early culling in dairy cattle. Clinical symptoms of Johne's disease include persistent diarrhea, inappetence, and resultant weight loss due to chronic inflammation of the small intestine. A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6479-6486.2003
更新日期:2003-11-01 00:00:00
abstract::Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.737-740.1984
更新日期:1984-09-01 00:00:00
abstract::A complex of trehalose-6, 6'-dimycolate (cord factor) and methylated bovine serum albumin was prepared, and its effect on the toxicity of cord factor in mice was studied. Either the active immunization with cord factor-methylated bovine serum albumin complex or the passive transfer of sera of rabbits vaccinated with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.2.203-212.1972
更新日期:1972-02-01 00:00:00
abstract::Toxic shock syndrome-associated staphylococcal and streptococcal exotoxins were tested for an ability to induce the production of tumor necrosis factor (TNF). Staphylococcal enterotoxins B and C1, along with streptococcal pyrogenic exotoxin A, all induced TNF production in a dose-dependent manner, with production peak...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.291-294.1989
更新日期:1989-01-01 00:00:00
abstract::Infection with tissue-migrating helminths is frequently associated with intense granulocyte infiltrations. Several host-derived factors are known to mediate granulocyte recruitment to the tissues, but less attention has been paid to how parasite-derived products trigger this process. Parasite-derived chemotactic facto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.4007-4018.2001
更新日期:2001-06-01 00:00:00
abstract::Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins, F and HN, were separated from Triton X-100- or Nonidet P-40-solubilized envelopes as unadsorbed and eluted fractions, respectively, by using glutaralde-hyde-treated chicken erythrocytes. These separated glycoproteins were biologically active. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.212-218.1980
更新日期:1980-10-01 00:00:00