Abstract:
:Changes in periodontal status are associated with shifts in the composition of the bacterial community in the periodontal pocket. The relative abundances of several newly recognized microbial species, including Filifactor alocis, as-yet-unculturable organisms, and other fastidious organisms have raised questions on their impact on disease development. We have previously reported that the virulence attributes of F. alocis are enhanced in coculture with Porphyromonas gingivalis. We have evaluated the proteome of host cells and F. alocis during a polymicrobial infection. Coinfection of epithelial cells with F. alocis and P. gingivalis strains showed approximately 20% to 30% more proteins than a monoinfection. Unlike F. alocis ATCC 35896, the D-62D strain expressed more proteins during coculture with P. gingivalis W83 than with P. gingivalis 33277. Proteins designated microbial surface component-recognizing adhesion matrix molecules (MSCRAMMs) and cell wall anchor proteins were highly upregulated during the polymicrobial infection. Ultrastructural analysis of the epithelial cells showed formation of membrane microdomains only during coinfection. The proteome profile of epithelial cells showed proteins related to cytoskeletal organization and gene expression and epigenetic modification to be in high abundance. Modulation of proteins involved in apoptotic and cell signaling pathways was noted during coinfection. The enhanced virulence potential of F. alocis may be related to the differential expression levels of several putative virulence factors and their effects on specific host cell pathways.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Aruni AW,Zhang K,Dou Y,Fletcher Hdoi
10.1128/IAI.01727-14subject
Has Abstractpub_date
2014-08-01 00:00:00pages
3261-74issue
8eissn
0019-9567issn
1098-5522pii
IAI.01727-14journal_volume
82pub_type
杂志文章abstract::Candida-associated denture stomatitis (DS) is a persistent and chronic oral infection of the denture-bearing palatal mucosa. DS stems from the ability of the fungal opportunistic pathogen Candida albicans to adhere to denture material and invade palatal tissue. Although DS is the most prevalent form of oral candidiasi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00617-19
更新日期:2019-11-18 00:00:00
abstract::Regulation of the synthesis of Vi polysaccharide, a major virulence determinant in Salmonella enterica serotype Typhi, is under the control of two regulatory systems, ompR-envZ and rscB-rscC, which respond to changes in osmolarity. Some serotype Typhi strains exhibit overexpression of Vi polysaccharide, which masks cl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00888-06
更新日期:2007-03-01 00:00:00
abstract::It was found that cells of Nocardia asteroides GUH-2 (virulent) were approximately 10 times more virulent than cells of N. asteroides 14759 (intermediate) and greater that 500 times more virulent than N. asteroides 10905 (avirulent) cells when early-stationary-phase cultures suspended in saline were injected intraven...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.995-1004.1977
更新日期:1977-06-01 00:00:00
abstract::Saccharomyces boulardii is a nonpathogenic yeast that protects against antibiotic-associated diarrhea and recurrent Clostridium difficile colitis. The administration of C. difficile toxoid A by gavage to S. boulardii-fed BALB/c mice caused a 1.8-fold increase in total small intestinal immunoglobulin A levels (P = 0.00...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2762-2765.2001
更新日期:2001-04-01 00:00:00
abstract::The ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent Rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (Microtus pennsylvanicus) was studied by transmission electron microscopy. In sections of epoxy-embedded specimens stained with u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1252-1260.1983
更新日期:1983-09-01 00:00:00
abstract::We have identified a Crp/Fnr-like transcriptional regulator of Streptococcus pyogenes that when inactivated attenuates virulence. The gene, named srv for streptococcal regulator of virulence, encodes a 240-amino-acid protein with 53% amino acid similarity to PrfA, a transcriptional activator of virulence in Listeria m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.3.1799-1803.2004
更新日期:2004-03-01 00:00:00
abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00716-15
更新日期:2015-09-01 00:00:00
abstract::P.69 pertactin (P.69 Prn), an adhesion molecule from the causative agent of pertussis, Bordetella pertussis, is present in cellular and most acellular vaccines that are currently used worldwide. Although both humoral immunity and cellular immunity directed against P.69 Prn have been implicated in protective immune mec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00769-08
更新日期:2009-02-01 00:00:00
abstract::Insertional mutagenesis studies of mrpB, a putative pilin-encoding open reading frame of the mrp gene cluster, which encodes mannose-resistant Proteus-like (MR/P) fimbriae of Proteus mirabilis, indicate that MrpB functions as the terminator for fimbrial assembly. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1759-1763.1998
更新日期:1998-04-01 00:00:00
abstract::We developed a human inhibition monoclonal enzyme-linked immunosorbent assay (HIMELISA) to investigate the human immune response to the lipooligosaccharides (LOS) of Neisseria meningitidis. Monoclonal antibodies (MAb) were used to define seven epitopes on four LOS molecules of a meningococcal strain (126E) previously ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2204-2213.1990
更新日期:1990-07-01 00:00:00
abstract::Iron is limiting in the human host, and bacterial pathogens respond to this environment by regulating gene expression through the ferric uptake regulator protein (Fur). In vitro studies have demonstrated that Neisseria gonorrhoeae controls the expression of several critical genes through an iron- and Fur-mediated mech...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4281-4287.2005
更新日期:2005-07-01 00:00:00
abstract::Urogenital Chlamydia serovars replicating in reproductive epithelium pose a unique challenge to host immunity and vaccine development. Previous studies have shown that CD4 T cells are necessary and sufficient to clear primary Chlamydia muridarum genital tract infections in the mouse model, making a protective CD4 T ce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06339-11
更新日期:2012-06-01 00:00:00
abstract::We studied the influence of staphylococcal toxic shock syndrome toxin 1 and streptococcal erythrogenic (pyrogenic) toxin A (ETA) on intact and digitonin-permeabilized human polymorphonuclear granulocytes (PMNs). As was shown by reversed-phase high-performance liquid chromatography analysis, toxic shock syndrome toxin ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.1055-1061.1993
更新日期:1993-03-01 00:00:00
abstract::Toxic shock syndrome (TSS) toxin 1 (TSST1) is produced by strains of Staphylococcus aureus associated with TSS. Purified TSST1 induces in rabbits a shock-like illness with many features similar to TSS in humans. These symptoms were also induced by TSST1-producing bacteria in diffusion chambers implanted in the rabbit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.304-309.1985
更新日期:1985-10-01 00:00:00
abstract::We examined the association of Herpesvirus saimiri with lymphocyte subsets and the functional integrity and distribution of these populations in owl monkeys with chronic, disease-free infections, in uninoculated, control animals, and in one monkey with H. saimiri-induced lymphoma. The lymphocyte subpopulations examine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.698-706.1981
更新日期:1981-05-01 00:00:00
abstract::The inhibitory effects of cortisol on cellular immunity were studied in vitro by using hamster peritoneal exudate cells. Two obligate, intracellular protozoa--Toxoplasma gondii and Besnoitia jellisoni-- were used to control for specificity of effects. Results indicate that immune lymphocytes specifically confer immuni...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.855-862.1977
更新日期:1977-03-01 00:00:00
abstract::Conditions are described for the production of an extracellular cytotoxin or CHO cell-killing factor by Vibrio fluvialis, a recently recognized enteric pathogen. The cell-killing factor was ammonium sulfate precipitable, heat labile, and inactivated by proteases, and had an isoelectric point (estimated by sucrose dens...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.773-777.1984
更新日期:1984-12-01 00:00:00
abstract::Encapsulated cells of Cryptococcus neoformans are potent activators of the alternative complement pathway. Previous studies found that monoclonal antibodies (MAbs) specific for the major capsular polysaccharide, termed glucuronoxylomannan (GXM), can markedly suppress the ability of the capsule to accumulate C3 from no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1547-1553.1998
更新日期:1998-04-01 00:00:00
abstract::YopE is a virulence factor that is secreted into host cells infected by Yersinia species. The YopE C-terminal domain has GTPase-activating protein (GAP) activity. The YopE N-terminal domain contains an epitope that is an immunodominant CD8(+) T cell antigen during primary infection of C57BL/6 mice with Yersinia pseudo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01687-14
更新日期:2014-07-01 00:00:00
abstract::A genomic library of meningococcal DNA from a clinical isolate of Neisseria meningitidis was constructed in the expression vector lambda gt11. Outer membrane complex was prepared from the same strain and used to immunize rabbits to raise polyclonal anti-outer membrane complex serum. The amplified library was probed wi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.11.2734-2740.1987
更新日期:1987-11-01 00:00:00
abstract::The microbicidal myeloperoxidase (MPO)-H2O2-chloride system strongly inhibits Escherichia coli DNA synthesis. Also, cell envelopes from MPO-treated E. coli cells lose their ability to interact with hemimethylated DNA sequences of oriC, the chromosomal origin of replication, raising the prospect that suppression of DNA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2655-2659.1998
更新日期:1998-06-01 00:00:00
abstract::Immunoglobulin A1 (IgA1) proteases are produced by a number of different species of bacteria which cause infection at human mucosal surfaces. The sole substrate of these proteases is human IgA1. Cleavage is within the hinge region of IgA1, although there is variability in the exact peptide bond within the hinge region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.320-331.1990
更新日期:1990-02-01 00:00:00
abstract::The Th1/Th2 cytokines involved in human periodontitis remain unclear; therefore, we established a humanized mouse model to investigate this issue in Actinobacillus actinomycetemcomitans-mediated periodontal infection. Quantitative-PCR analysis clearly demonstrates a predominantly mixed Th1 and Th2 expression profile a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5269-5273.2002
更新日期:2002-09-01 00:00:00
abstract::Indomethacin, a nonsteroidal anti-inflammatory agent, will abolish salmonella-induced rabbit ileal secretion when given prior to the establishment of infection. In the present study, we assessed whether indomethacin can inhibit salmonella-induced intestinal secretion when administered after infection and net intestina...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.1.136-139.1977
更新日期:1977-07-01 00:00:00
abstract::Human gamma delta T cells are known to respond at high frequencies to pathogenic mycobacteria. Here we show that opportunistic strains of mycobacteria share with pathogenic mycobacteria the ability to trigger at high frequencies human V gamma 9V delta 2 T-cell-receptor-positive T lymphocytes. Stimulating ligands were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2753-2757.1992
更新日期:1992-07-01 00:00:00
abstract::To identify potential vaccine candidates for the prevention of infection with the filarial nematode Onchocerca volvulus, we screened an O. volvulus L3 stage cDNA library with sera from putatively immune (PI) subjects, and a prominent immunogenic clone of 1,184 nucleotides was identified. It contained an open reading f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.851-858.2002
更新日期:2002-02-01 00:00:00
abstract::The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the concl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.262-264.1986
更新日期:1986-10-01 00:00:00
abstract::Enterococcus faecalis is a highly stress resistant opportunistic pathogen. The intrinsic ruggedness of this bacterium is supposed to be the basis of its capacity to colonize the hostile environments of hospitals and to cause several kinds of infections. We show in this work that general resistance to very different en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01299-12
更新日期:2013-08-01 00:00:00
abstract::Listeria monocytogenes is a ubiquitous potentially pathogenic organism requiring iron for growth and virulence. Although it does not produce siderophores, L. monocytogenes is able to obtain iron by using either exogenous siderophores produced by various microorganisms or natural catechol compounds widespread in the en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2778-2785.1997
更新日期:1997-07-01 00:00:00
abstract::Phagocytosis and mechanisms of killing of Aspergillus fumigatus conidia by murine alveolar macrophages (AM), which are the main phagocytic cells of the innate immunity of the lung, were investigated. Engulfment of conidia by murine AM lasts 2 h. Killing of A. fumigatus conidia by AM begins after 6 h of phagocytosis. S...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3034-3042.2003
更新日期:2003-06-01 00:00:00