Expression of the cloned toxic shock syndrome toxin 1 gene (tst) in vivo with a rabbit uterine model.

Abstract:

:Toxic shock syndrome (TSS) toxin 1 (TSST1) is produced by strains of Staphylococcus aureus associated with TSS. Purified TSST1 induces in rabbits a shock-like illness with many features similar to TSS in humans. These symptoms were also induced by TSST1-producing bacteria in diffusion chambers implanted in the rabbit uterus. Naturally occurring TSST1+ strains and a TSST1- strain harboring a pE194-derived plasmid carrying the cloned TSST1 determinant tst gave the same symptoms. TSST1- strains and a TSST1- strain carrying a pE194-tst plasmid with a deletion of the tst gene had no effect in rabbits. The results with the plasmid-carrying TSST1+ and TSST1- strains, which were isogenic apart from tst, show that the toxin is responsible for the illness in rabbits and suggest that it is a major factor in the pathogenesis of TSS.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

de Azavedo JC,Foster TJ,Hartigan PJ,Arbuthnott JP,O'Reilly M,Kreiswirth BN,Novick RP

doi

10.1128/IAI.50.1.304-309.1985

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

304-9

issue

1

eissn

0019-9567

issn

1098-5522

journal_volume

50

pub_type

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