Abstract:
:LipL32 is the major outer membrane protein in pathogenic Leptospira. It is highly conserved throughout pathogenic species and is expressed in vivo during human infection. While these data suggest a role in pathogenesis, a function for LipL32 has not been defined. Outer membrane proteins of gram-negative bacteria are the first line of molecular interaction with the host, and many have been shown to bind host extracellular matrix (ECM). A search for leptospiral ECM-interacting proteins identified the major outer membrane protein, LipL32. To verify this finding, recombinant LipL32 was expressed in Escherichia coli and was found to bind Matrigel ECM and individual components of ECM, including laminin, collagen I, and collagen V. Likewise, an orthologous protein found in the genome of Pseudoalteromonas tunicata strain D2 was expressed and found to be functionally similar and immunologically cross-reactive. Lastly, binding activity was mapped to the C-terminal 72 amino acids. These studies show that LipL32 and an orthologous protein in P. tunicata are immunologically cross-reactive and function as ECM-interacting proteins via a conserved C-terminal region.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hoke DE,Egan S,Cullen PA,Adler Bdoi
10.1128/IAI.01643-07subject
Has Abstractpub_date
2008-05-01 00:00:00pages
2063-9issue
5eissn
0019-9567issn
1098-5522pii
IAI.01643-07journal_volume
76pub_type
杂志文章abstract::Some interrelationships among age, ambient temperature, intestinal transit, and enterotoxigenic Escherichia coli infection were studied in an infant mouse model. The transit of dye in the small intestine was accelerated during the response to heat-stable E. coli enterotoxin. Transit in the small intestine of normal mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.127-132.1979
更新日期:1979-07-01 00:00:00
abstract::Burkholderia pseudomallei, a causative agent of melioidosis, is a Gram-negative facultative intracellular bacterium that can survive and multiply in macrophages. Previously, we demonstrated that B. pseudomallei failed to activate gene expression downstream of the MyD88-independent pathway, particularly the expression ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01254-10
更新日期:2011-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3604-3608.2004
更新日期:2004-06-01 00:00:00
abstract::To assess the humoral immunological responses at the subclass level in shigellosis, specific antibody responses against Shigella dysenteriae 1 lipopolysaccharide (LPS), S. flexneri Y LPS, invasion plasmid-coded protein antigens (Ipa), and Shiga toxin were analyzed. Antibody responses of 41 patients with S. dysenteriae...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.2054-2061.1995
更新日期:1995-05-01 00:00:00
abstract::Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02790-14
更新日期:2015-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2445-2452.1993
更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1895-1899.1987
更新日期:1987-08-01 00:00:00
abstract::Using a PCR-based strategy and degenerate oligonucleotides, we isolated a Legionella pneumophila gene that showed high sequence similarity to members of the fliI gene family. An insertion mutation that disrupted the fliI open reading frame was recombined onto the L. pneumophila chromosome and analyzed for its effects ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2497-2501.1997
更新日期:1997-06-01 00:00:00
abstract::Most strains of Shigella flexneri 2a and enteroaggregative Escherichia coli carry a highly conserved chromosomal locus which encodes a 109-kDa secreted mucinase (called Pic) and, on the opposite strand in overlapping fashion, an oligomeric enterotoxin called ShET1, encoded by the setA and setB genes. Here, we characte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2915-2925.2002
更新日期:2002-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.303-307.1994
更新日期:1994-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.6618-6624.2001
更新日期:2001-11-01 00:00:00
abstract::Toxic shock syndrome-associated staphylococcal and streptococcal exotoxins were tested for an ability to induce the production of tumor necrosis factor (TNF). Staphylococcal enterotoxins B and C1, along with streptococcal pyrogenic exotoxin A, all induced TNF production in a dose-dependent manner, with production peak...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.291-294.1989
更新日期:1989-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1661-1664.1988
更新日期:1988-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00660-08
更新日期:2008-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2716-2723.1996
更新日期:1996-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1549-1553.1976
更新日期:1976-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4512-4520.2004
更新日期:2004-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3512-3520.2005
更新日期:2005-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1107-1115.1999
更新日期:1999-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00991-07
更新日期:2007-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00374-19
更新日期:2019-10-18 00:00:00
abstract::While gamma/delta T cells are involved in host defense and immunopathology in a variety of infectious diseases, their precise role is not yet clearly defined. In the absence of gamma/delta T cells, mice die after infection with a dose of Listeria monocytogenes that is not lethal in immunologically intact animals. Morb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7213-7223.2001
更新日期:2001-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.526-529.1978
更新日期:1978-05-01 00:00:00
abstract::The possibility that soluble products of Schistosoma mansoni eggs might participate in the pathogenesis of hepatic fibrosis in schistosomiasis was investigated. Both crude saline extracts of eggs (soluble egg antigen [SEA]) and a partially purified SEA fraction contained activity which stimulated guinea pig and human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.103-108.1982
更新日期:1982-10-01 00:00:00
abstract::Oxygen consumption by Treponema pallidum was observed to be glucose dependent. Treponemes consumed O2 to a low dissolved O2 concentration of 0.01 mumol of O2 per ml when respiration ceased. A fermentative degradation of glucose occurred during the maintenance of treponemes at 0.01 mumol of O2 per ml. Although the init...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.992-997.1981
更新日期:1981-03-01 00:00:00
abstract::The binding of cryptococcal polysaccharide to a non-encapsulated strain of Cryptococcus neoformans was studied. Binding of purified polysaccharide to the yeast was determined by inhibition of phagocytosis and by indirect immunofluorescence techniques. The ability of cryptococcal polysaccharide to prevent phagocytosis ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.99-106.1977
更新日期:1977-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.26-33.1977
更新日期:1977-01-01 00:00:00
abstract::Vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. Gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01305-15
更新日期:2016-06-23 00:00:00
abstract::L929 cell populations persistently infected for more than 600 days exhibited cell cycle distributions and generation times similar to those of uninfected cells. The RNA and protein contents of these long-term-infected cells, as determined by flow cytometry and correlated with the cell cycle, were likewise approximatel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1731-1733.1987
更新日期:1987-07-01 00:00:00