Abstract:
:Vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. Gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medicine. In this study, we have characterized the endothelial cell response to the common bacterium Fusobacterium nucleatum, an important bridging species that facilitates the activity of late colonizers of the dental biofilm. Endothelial cells were infected with Fusobacterium nucleatum (strain 25586) for periods of 4, 12, 24, or 48 h. Cell proliferation and tube formation were analyzed, and expression of adhesion molecules (CD31 and CD34) and vascular endothelial growth factor (VEGF) receptors 1 and 2 was measured by fluorescence-activated cell sorter (FACS) analysis. Data indicate that F. nucleatum impaired endothelial cell proliferation and tube formation. The findings suggest that the modified endothelial cell response acts as a mechanism promoting the pathogenic progression of periodontal diseases and may potentially suggest the involvement of periodontopathogens in systemic diseases associated with periodontal inflammation.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Mendes RT,Nguyen D,Stephens D,Pamuk F,Fernandes D,Van Dyke TE,Kantarci Adoi
10.1128/IAI.01305-15subject
Has Abstractpub_date
2016-06-23 00:00:00pages
2141-2148issue
7eissn
0019-9567issn
1098-5522pii
IAI.01305-15journal_volume
84pub_type
杂志文章abstract::Three monoclonal antibodies (MAbs), all of the immunoglobulin G1 subclass, were raised against Cryptococcus neoformans by using the technique of cyclophosphamide ablation of B-cell responses against shared epitopes of the cross-reactive fungus Trichosporon beigelii. MAb 3C2 was reactive against the encapsulated and no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.143-149.1992
更新日期:1992-01-01 00:00:00
abstract::Trichinella spiralis creates a unique intracellular habitat in striated muscle. We report that a proteoglycan, syndecan-1, is induced early in infection yet is not essential for habitat development and exerts a modest influence on the immune response. This report is the first to address the requirement for a specific ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1941-1943.2006
更新日期:2006-03-01 00:00:00
abstract::A Clostridium difficile isolate was found to produce an actin-specific ADP-ribosyltransferase (CDT) homologous to the enzymatic components of Clostridium perfringens iota toxin and Clostridium spiroforme toxin (M. R. Popoff, E. J. Rubin, D. M. Gill, and P. Boquet, Infect. Immun. 56:2299-2306, 1988). The CDT locus from...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1402-1407.1997
更新日期:1997-04-01 00:00:00
abstract::The immunogenicity of the two major group 19 pneumococcal polysaccharides, types 19F and 19A, was studied in human volunteers. Antibody responses after vaccination with either 50 micrograms of type 19F polysaccharide, 50 micrograms of type 19A polysaccharide, or 25 micrograms of both polysaccharides premixed in a sing...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/IAI.36.3.1261-1262.1982
更新日期:1982-06-01 00:00:00
abstract::The affinity of uropathogenic Escherichia coli to kidneys and bladders of experimentally infected mice was shown to be determined in part by the adhesive properties of the infecting bacteria. Mice were infected with various pairwise combinations of two homogeneic sets of bacteria: (i) mutants derived from a human pyel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.265-272.1983
更新日期:1983-04-01 00:00:00
abstract::In this study we have investigated the antibody and CD4 T-cell responses to the well-characterized malaria vaccine candidate MSP-1 during the course of a primary Plasmodium chabaudi chabaudi (AS) infection. Specific antibody responses can be detected within the first week of infection, and CD4 T cells can be detected ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2245-2251.2001
更新日期:2001-04-01 00:00:00
abstract::Avian pathogenic Escherichia coli (APEC) cause extraintestinal disease in avian species via respiratory tract infection. Virulence factors associated with APEC include type 1 and P fimbriae, curli, aerobactin, lipopolysaccharide (LPS), K1 capsular antigen, temperature-sensitive hemagglutinin (Tsh), and an uncharacteri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.494-503.2003
更新日期:2003-01-01 00:00:00
abstract::Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.243-248.1971
更新日期:1971-02-01 00:00:00
abstract::A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2732-2736.1993
更新日期:1993-06-01 00:00:00
abstract::The innate immune response in human tuberculosis is not completely understood. To improve our knowledge regarding the role of cathelicidin hCAP-18/LL37 in the innate immune response to tuberculosis infection, we used immunohistochemistry, immunoelectron microscopy, and gene expression to study the induction and produc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01218-07
更新日期:2008-03-01 00:00:00
abstract::Culture fluids after growth of Mycobacterium bovis BCG on Sauton medium contain actively secreted proteins and proteins released by bacterial lysis. BCG culture fluids and sonicates of Mycobacterium tuberculosis and Mycobacterium paratuberculosis were tested after separation by gel filtration and sodium dodecyl sulfat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.289-296.1998
更新日期:1998-01-01 00:00:00
abstract::Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1420-1425.1996
更新日期:1996-04-01 00:00:00
abstract::The mechanism of in vitro synergistic lysis of sheep erythrocytes by Corynebacterium ovis and Corynebacterium equi was investigated. Hemolysis required (i) the action of phospholipase D from C. ovis, (ii) the action of an extracellular protein of C. equi, and (iii) Mg2+. Maximum lysis required imposition on the system...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.123-131.1980
更新日期:1980-07-01 00:00:00
abstract::The adenylate cyclase-hemolysin of Bordetella pertussis consists of a cell-invasive N-terminal adenylate cyclase domain linked to a C-terminal RTX hemolysin containing extensive glycine-rich repeats. The toxin is an essential virulence factor required in the initial stages of infection. Adenylate cyclase-hemolysin was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3309-3315.1995
更新日期:1995-09-01 00:00:00
abstract::Enterococcus faecalis is a highly stress resistant opportunistic pathogen. The intrinsic ruggedness of this bacterium is supposed to be the basis of its capacity to colonize the hostile environments of hospitals and to cause several kinds of infections. We show in this work that general resistance to very different en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01299-12
更新日期:2013-08-01 00:00:00
abstract::Campylobacter rectus is a putative periodontopathogen which expresses a proteinaceous surface layer (S-layer) external to the outer membrane. S-layers are considered to play a protective role for the microorganism in hostile environments. The S-layer proteins from six different C. rectus strains (five human isolates a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.478-483.1997
更新日期:1997-02-01 00:00:00
abstract::We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and Palo-Alto(FUP)1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::The ability of the endospore-forming, gram-positive bacterium Bacillus anthracis to survive in activated macrophages is key to its germination and survival. In a previous publication, we discovered that exposure of primary murine macrophages to B. anthracis endospores upregulated NOS 2 concomitant with an .NO-dependen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00283-07
更新日期:2007-08-01 00:00:00
abstract::Peptidoglycans of Leptotrichia buccalis ATCC 14201 and ATCC 19616 were isolated by extraction with sodium dodecyl sulfate and subsequent digestion of the sodium dodecyl sulfate-insoluble residue with proteases and alpha-amylase. Cell wall fractions obtained by sodium dodecyl sulfate extraction and protease digestion w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.132-136.1983
更新日期:1983-01-01 00:00:00
abstract::A dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. Its effect on other human pathogens was investigated. A 10% serum solution increased peak O2 consumption for Escherichia coli and Staphylococcus aureus to 157% (P less than 0.05) and 199% (P less than 0.02), respectively, of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.657-663.1986
更新日期:1986-06-01 00:00:00
abstract::The adipocytokine leptin links nutritional status to immune function. Leptin signaling protects from amebiasis, but the molecular mechanism is not understood. We developed an in vitro model of ameba-host cell interaction to test the hypothesis that leptin prevents ameba-induced apoptosis in host epithelial cells. We d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06140-11
更新日期:2012-05-01 00:00:00
abstract::The type III secretion system (T3SS) plays a crucial role in the pathogenesis of many Gram-negative bacteria, including Edwardsiella tarda, an important fish pathogen. Within the E. tarda T3SS, there are three proteins (EsaB/EsaL/EsaM) that are homologous to proteins present in many other bacteria, including SpiC/SsaL...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00322-17
更新日期:2017-08-18 00:00:00
abstract::Clostridium difficile is recognized as the major cause of antibiotic-associated colitis. C. difficile produces two toxins, A (enterotoxin) and B (cytotoxin), that are implicated in the pathogenesis of the colitis. We examined the dose responses, time course, and synergism of these two toxins in ligated rabbit intestin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.3.582-588.1988
更新日期:1988-03-01 00:00:00
abstract::Monoclonal antibodies to the capsular polysaccharide of Cryptococcus neoformans produce distinct capsular reactions and have biological activities that are determined by serotype specificity. In the present study, polyclonal rabbit anticapsular antibodies were cross-absorbed to produce serotype specificities similar t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.1189-1191.2001
更新日期:2001-02-01 00:00:00
abstract::Purification of the envelope antigen of Yersinia pestis EV with passive hemagglutination activity is described. The purification procedure consisted of pancreatin digestion, chromatography on human erythrocyte stroma set on Celite, and rechromatography on Sephadex G-200. Chemical, physical, and biological properties o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::The chlamydial inclusion occupies a unique niche within the eukaryotic cell that does not interact with endocytic compartments but instead is fusogenic with a subset of sphingomyelin-containing exocytic vesicles. The Chlamydia trachomatis inclusion acquires these distinctive properties by as early as 2 h postinfection...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.973-984.2003
更新日期:2003-02-01 00:00:00
abstract::The indigenous microbial flora of the middle ear cavity of Mongolian gerbils, Meriones unguiculatus, was isolated, characterized, and identified, showing it to be sparse and transitory. Organisms, when found in the middle ear cavity, were most likely to be Staphylococcus epidermidis-like organisms. Cerumen from the ex...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1113-1118.1981
更新日期:1981-06-01 00:00:00
abstract::Epitope mapping of outer surface protein C (OspC) by using sera from patients with neuroborreliosis led to the identification of one single major immunodominant epitope within the C-terminal 10 amino acid residues. Peptide binding studies and alanine replacement scanning of the C-terminal decapeptide, PVVAESPKKP, reve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::To characterize the mechanism by which interleukin 10 (IL-10) inhibits Th1 responses to intracellular pathogens, we evaluated the interaction between IL-10 and Mycobacterium tuberculosis-induced gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells from persons across the spectrum of tuberculou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.913-918.1996
更新日期:1996-03-01 00:00:00
abstract::Newly hatched chicks were vaccinated orally with a genetically defined Salmonella enteritidis aroA candidate, strain CVL30. In chickens immunized with 10(5) or 10(9) CFU and challenged by the intravenous route with 10(8) CFU of S. enteritidis 109 Nalr at 8 weeks old, there were similar reductions in colonization of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4747-4754.1994
更新日期:1994-11-01 00:00:00