Abstract:
:For the first time, hepatitis A viral antigen (HAAg) was shown in liver biopsy tissue from a patient in the acute phase of hepatitis type A by light and electron microscopy, using the peroxidase-antibody technique. Under light microscopy, the staining for HAAg appeared as a fine, granular reaction product, scattered throughout the cytoplasm of hepatocytes and sinusoidal lining cells. Standard thin-section electron microscopy revealed virus-like particles, 24 to 27 nm in diameter, in cytoplasmic vesicles of hepatocytes and Kupffer cells. By immunoperoxidase electron microscopy, HAAg was detected on particles aggregated within cytoplasmic vesicles of hepatocytes, thus demonstrating that the virus-like particles (24 to 27 nm) are hepatitis A virus. The surrounding membrane of the vesicles was also positive for HAAg. The distribution patterns of HAAg in human liver were virtually identical to those described for experimentally infected marmosets. It is notable that most HAAg was detected within vesicles of liver cell cytoplasm, suggesting the possibility of vesicle-oriented morphogenesis of hepatitis A virus.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Shimizu YK,Shikata T,Beninger PR,Sata M,Setoyama H,Abe H,Tanikawa Kdoi
10.1128/IAI.36.1.320-324.1982subject
Has Abstractpub_date
1982-04-01 00:00:00pages
320-4issue
1eissn
0019-9567issn
1098-5522journal_volume
36pub_type
杂志文章abstract::Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00139-16
更新日期:2016-06-23 00:00:00
abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4400-4406.1999
更新日期:1999-09-01 00:00:00
abstract::Antibodies against the Plasmodium falciparum P0 ribosomal phosphoprotein (PfP0) have been detected exclusively but extensively in malaria-immune persons. Polyclonal rabbit and mice sera were raised against two recombinant polypeptides of P. falciparum P0 protein, PfP0N and PfP0C, covering amino acids 17 to 61 and the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00
abstract::The glycolipid haptens of Mycoplasma pneumoniae became immunogenic when bound to membrane proteins of Acholeplasma laidlawii by reaggregation. This process consisted of the solubilization of lipid-depleted A. laidlawii membranes and M. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed so...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.420-423.1971
更新日期:1971-03-01 00:00:00
abstract::Bovine septicemic Escherichia coli 31A agglutinates bovine, rabbit, and human erythrocytes and adheres in vitro to the brush border of bovine or ovine intestinal epithelial cells and to the human colon carcinoma Caco-2 cell line. The adhesion and hemagglutination of E. coli 31A are mediated by a chromosome-encoded fim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.332-342.1996
更新日期:1996-01-01 00:00:00
abstract::A significant clinical complication of pulmonary infections with Klebsiella pneumoniae is peripheral blood dissemination, resulting in a systemic infection concurrent with the localized pulmonary infection. In this context, little is known about the role of tumor necrosis factor receptor 1 (TNFR1)-mediated innate immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.4891-4900.2003
更新日期:2003-09-01 00:00:00
abstract::FcRgamma and interleukin-10 (IL-10) are both required for chronic disease in C57BL/6 mice with Leishmania mexicana parasite infection. FcRgamma is a component of several different FcRs and may be a component of some T-cell receptors. The initial antibody response to L. mexicana is an immunoglobulin G1 (IgG1) response,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-07
更新日期:2008-02-01 00:00:00
abstract::During acute infection with bacteria, viruses or parasites, a fraction of macrophages engulf large numbers of red and white blood cells, a process called hemophagocytosis. Hemophagocytes persist into the chronic stage of infection and have an anti-inflammatory phenotype. Salmonella enterica serovar Typhimurium infecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01149-15
更新日期:2015-10-12 00:00:00
abstract::Among a number of laboratory strains of Listeria monocytogenes used in experimental infection, strain LO28 is highly capable of inducing robust beta interferon (IFN-β) production in infected macrophages. In this study, we investigated the molecular mechanism of the IFN-β-inducing ability of LO28 by comparing it with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06162-11
更新日期:2012-07-01 00:00:00
abstract::Akabane virus (a Bunyavirus) has been associated with epizootics of congenital deformities in cattle, sheep, and goats. Experimental studies using mouse-adapted virus inoculated intravenously into pregnant sheep gave an inapparent infection. Neutralizing antibodies were detected on day 5, and peaks in the titer were s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.254-262.1977
更新日期:1977-01-01 00:00:00
abstract::Candida albicans is a major opportunistic pathogen in immunocompromised patients. Production of proinflammatory cytokines by host cells in response to C. albicans plays a critical role in the activation of immune cells and final clearance of the organism. Invasion of host cells and tissues is considered one of the vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4588-4595.2005
更新日期:2005-08-01 00:00:00
abstract::Chlamydia trachomatis is an obligate intracellular pathogen that causes sexually transmitted disease. In women, chlamydial infections may cause pelvic inflammatory disease (PID), ectopic pregnancy, and infertility. The role of antibodies in protection against a primary Chlamydia infection is unclear and was a focus of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00413-20
更新日期:2020-12-15 00:00:00
abstract::A gene encoding the cytotoxic enterotoxin (Act) from Aeromonas hydrophila was hyperexpressed with the pET, pTRX, and pGEX vector systems. Maximum toxin yield was obtained with the pTRX vector. Approximately 40 to 60% of Act was in a soluble form with the pTRX and pET vector systems. The toxin protein was purified to h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4299-4308.1997
更新日期:1997-10-01 00:00:00
abstract::An Escherichia coli oligonucleotide microarray based on three sequenced genomes was validated for comparative genomic microarray hybridization and used to study the diversity of E. coli O157 isolates from human infections and food and animal sources. Among 26 test strains, 24 (including both Shiga toxin [Stx]-positive...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00956-07
更新日期:2008-02-01 00:00:00
abstract::We found that when group A streptococci are cocultured with human pharyngeal cells, they upregulate and secrete a 25-kDa toxin, determined to be the bacteriophage-encoded streptococcal pyrogenic exotoxin C (SpeC). This prompted us to determine if the bacteriophage themselves are induced during coculture conditions. We...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1440-1443.2001
更新日期:2001-03-01 00:00:00
abstract::Leptospirosis can activate inflammatory responses through Toll-like receptors (TLRs) and may cause renal tubulointerstitial fibrosis characterized by the accumulation of extracellular matrix (ECM). We have previously demonstrated that Leptospira santorosai serovar Shermani detergent extract stimulates ECM accumulation...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01287-09
更新日期:2011-03-01 00:00:00
abstract::Burkholderia cenocepacia, a member of the B. cepacia complex, is an opportunistic pathogen that causes serious infections in patients with cystic fibrosis. We identified a six-gene cluster in chromosome 1 encoding a two-component regulatory system (BCAL2831 and BCAL2830) and an HtrA protease (BCAL2829) hypothesized to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01581-06
更新日期:2007-04-01 00:00:00
abstract::Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1281-1289.1990
更新日期:1990-05-01 00:00:00
abstract::We have previously shown that individuals infected with Chlamydia trachomatis can develop a robust antibody response to a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor [CPAF]). We now report that sera collected from these infected individuals neutralized the proteolytic act...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4414-4419.2005
更新日期:2005-07-01 00:00:00
abstract::Poly-N-acetyl-glucosamine (PNAG) is a staphylococcal surface polysaccharide influencing biofilm formation that is also under investigation for its vaccine potential. Antibodies that bind to PNAG with either low (<15%) or high (>90%) levels of acetate are superior at opsonic and protective activity compared with antibo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00078-07
更新日期:2007-07-01 00:00:00
abstract::The intravascular trematode Schistosoma mansoni is a causative agent of schistosomiasis, a disease that constitutes a major health problem globally. In this study we cloned and characterized the schistosome tegumental phosphodiesterase SmNPP-5 and evaluated its role in parasite virulence. SmNPP-5 is a 52.5-kDa protein...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05431-11
更新日期:2011-10-01 00:00:00
abstract::We used an in vitro opsonophagocytic killing assay to measure the functional activity of antibody directed at the mucoid exopolysaccharide (MEP) antigen expressed by Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Rabbit antibodies raised to purified MEP were able to mediate phagocytic killing i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.2.281-285.1985
更新日期:1985-08-01 00:00:00
abstract::Indoleamine 2,3-dioxygenase (IDO), a tryptophan-degrading enzyme, is inducible by various interferons (IFNs). IDO-mediated tryptophan degradation, but not the formation of IDO-catalyzed tryptophan metabolites, has been suggested as a mechanism for the antiparasitic action of IFN-gamma. To determine whether the IFN-gam...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.1810-1813.1993
更新日期:1993-05-01 00:00:00
abstract::Lung dendritic cells were identified by immunohistochemistry in lung tissue sections from C57BL/6 mice. Following isolation from the lungs using CD11c magnetic beads, the flow cytometric analysis of I-A(b+) and CD11c(+) cells indicated a mixed population of dendritic cells at different stages of maturation, with most ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.1127-1133.2001
更新日期:2001-02-01 00:00:00
abstract::A T antigen preparation free of trypsin was obtained by application of CNBr-Sepharose linked to pure trypsin. Purification on an ion exchange chromatography column results in an electrophoretically homogeneous preparation of T protein. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.993-994.1976
更新日期:1976-03-01 00:00:00
abstract::Filarial infection is initiated by mosquito-derived third-stage larvae (L3) deposited on the skin that transit through the epidermis, which contains Langerhans cells (LC) and keratinocytes (KC), among other cells. This earliest interaction between L3 and the LC likely conditions the priming of the immune system to the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01301-12
更新日期:2013-05-01 00:00:00
abstract::Because of the unavailability of a suitable antigen, specific in vitro lymphocyte reactivity has not been investigated in persons who have had systemic blastomycosis. Twelve persons who had recovered from blastomycosis were evaluated, and all were found to exhibit strongly positive and apparently specific in vitro cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.485-490.1981
更新日期:1981-08-01 00:00:00
abstract::To characterize the roles of Porphyromonas gingivalis and its components in disease processes, we investigated the cytokine profiles induced by live P. gingivalis, its lipopolysaccharide (LPS), and its major fimbrial protein, fimbrillin (FimA). A cytokine antibody array revealed that human monocyte-derived macrophages...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1204-1214.2006
更新日期:2006-02-01 00:00:00
abstract::Previous studies demonstrated that Mycoplasma arthritidis strain 158 acquired a high degree of virulence upon lysogenization with bacteriophage MAV1. In the present study, the association between MAV1 and virulence was reexamined by creating new lysogens of 158 and of a relatively avirulent mutant, strain 158-1. In th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7322-7325.2004
更新日期:2004-12-01 00:00:00
abstract::Recognition of microbial patterns by host receptors is the first step in a multistep sequence leading to neutrophil-dependent host resistance. Although the role of membrane-bound sensors in bacterial recognition has been examined in detail, the importance of cytosolic sensors in the lungs is largely unexplored. In thi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05641-11
更新日期:2011-11-01 00:00:00