Abstract:
:Borrelia burgdorferi, the causative agent of Lyme disease, loses its ability to infect and cause disease in mammalian hosts after repeated in vitro passage. To identify proteins preferentially expressed by the low-passage strain and thus representing potential virulence factors, the polypeptide profiles of virulent, low-passage and nonvirulent, high-passage forms of B. burgdorferi B31 were compared by nonequilibrium pH gradient two-dimensional gel electrophoresis. Four low-passage-associated proteins with relative molecular masses (M(r)s) of 35,000, 28,000, 24,000, and 20,000 were identified. Of these, the 28- and 35-kDa polypeptides were not expressed in detectable quantities in the high-passage B31 strain, whereas the 24- and 20-kDa proteins were present in reduced quantities. All four of these proteins were lipoproteins, as determined by labelling with [3H]palmitate. The abundant 28-kDa component, called outer surface protein D (OspD), is surface exposed on the basis of its proteolysis during treatment of intact organisms with proteinase K. The ospD gene is located on a 38-kb linear plasmid present in seven of nine low-passage strains of B. burgdorferi examined but absent in most high-passage, nonvirulent strains tested. Molecular cloning and sequence analysis of the ospD gene locus revealed an open reading frame encoding a 28,436-Da polypeptide with a putative signal peptidase II leader sequence. An unusual feature of the region upstream of the gene was the presence of seven contiguous, direct repeats of a 17-bp sequence that includes consensus -35 and -10 transcription initiation signals; however, only one transcription initiation site was active as determined by primer extension analysis. Further study of these and other polypeptides associated with low-passage strains may lead to identification of B. burgdorferi gene products required for infection and pathogenesis in mammalian hosts.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Norris SJ,Carter CJ,Howell JK,Barbour AGdoi
10.1128/IAI.60.11.4662-4672.1992keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
4662-72issue
11eissn
0019-9567issn
1098-5522journal_volume
60pub_type
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journal_title:Infection and immunity
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doi:10.1128/IAI.59.6.1991-1996.1991
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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journal_title:Infection and immunity
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doi:10.1128/IAI.66.12.5819-5825.1998
更新日期:1998-12-01 00:00:00
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更新日期:1999-11-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1982-02-01 00:00:00
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