Abstract:
:Pseudomonas aeruginosa can invade corneal epithelial cells and translocates multilayered corneal epithelia in vitro, but it does not penetrate the intact corneal epithelium in vivo. In healthy corneas, the epithelium is separated from the underlying stroma by a basement membrane containing extracellular matrix proteins and pores smaller than bacteria. Here we used in vivo and in vitro models to investigate the potential of the basement membrane to defend against P. aeruginosa. Transmission electron microscopy of infected mouse corneas in vivo showed penetration of the stroma by P. aeruginosa only where the basement membrane was visibly disrupted by scratch injury, suggesting that the intact basement membrane prevented penetration. This hypothesis was explored using an in vitro Matrigel Transwell model to mimic the corneal basement membrane. P. aeruginosa translocation of multilayered corneal epithelia grown on Matrigel was approximately 100-fold lower than that of cells grown without Matrigel (P < 0.005, t test). Matrigel did not increase transepithelial resistance. Matrigel-grown cells blocked translocation by a P. aeruginosa protease mutant. Without cells, Matrigel also reduced traversal of P. aeruginosa and the protease mutant. Fluorescence microscopy revealed a relative accumulation of bacteria at the superficial epithelium of cells grown on Matrigel at 3 h compared to cells grown on uncoated filters. By 5 h, bacteria accumulated beneath the cells, suggesting direct trapping by the Matrigel. These findings suggest that the basement membrane helps defend the cornea against infection via physical barrier effects and influences on the epithelium and that these roles could be compromised by P. aeruginosa proteases.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Alarcon I,Kwan L,Yu C,Evans DJ,Fleiszig SMdoi
10.1128/IAI.00111-09subject
Has Abstractpub_date
2009-08-01 00:00:00pages
3264-71issue
8eissn
0019-9567issn
1098-5522pii
IAI.00111-09journal_volume
77pub_type
杂志文章abstract::The initial encounter of Leishmania cells and cells from the immune system is fundamentally important in the outcome of infection and determines disease development or resistance. We evaluated the anti-Leishmania amazonensis response of naive volunteers by using an in vitro priming (IVP) system and comparing the respo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7453-7460.2001
更新日期:2001-12-01 00:00:00
abstract::Exposure of PAM cells, a spontaneously transformed mouse cell line, to brain homogenates from mice infected with scrapie caused a relative decrease in total cell yield, which persisted from passage 2 or 3 to passage 18 after treatment. The effect was elicited by each of the eight independent scrapie isolates tested. L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.163-167.1976
更新日期:1976-07-01 00:00:00
abstract::Escherichia coli hemolysin is considered an important virulence factor in extraintestinal E. coli infections. The present study demonstrates that cultured pulmonary artery endothelial cells are susceptible to attack by low concentrations of E. coli hemolysin (greater than or equal to 0.05 hemolytic units/ml; greater t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3796-3801.1990
更新日期:1990-11-01 00:00:00
abstract::Salmonella serovars are associated with human diseases that range from mild gastroenteritis to host-disseminated enteric fever. Human infections by Salmonella enterica serovar Typhi can lead to typhoid fever, but this serovar does not typically cause disease in mice or other animals. In contrast, S. enterica serovar T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1005-1013.2000
更新日期:2000-03-01 00:00:00
abstract::We have investigated the possibility that nitric oxide (NO) synthesis may affect the course of a trypanosome infection via T-cell responses using mice deficient in inducible NO synthase (iNOS). Parasitemia levels increased at the same rate in both iNOS-deficient homozygous and control heterozygous mice, and peak paras...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3334-3338.1999
更新日期:1999-07-01 00:00:00
abstract::To explore the mechanisms underlying the eosinophil-mediated inflammation of tropical pulmonary eosinophilia (TPE), bronchoalveolar lavage (BAL) fluid, serum, and supernatants from pulmonary and blood leukocytes (WBC) from patients with acute TPE (n = 6) were compared with those obtained from healthy uninfected indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1337-1342.2003
更新日期:2003-03-01 00:00:00
abstract::Light and electron microscopy with histochemical staining were used to estimate exopolysaccharide production by strains of viridans streptococci recovered from patients with endocarditis. Six strains were selected for study because they represented a wide range of in vitro polysaccharide production. By light microscop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.359-367.1984
更新日期:1984-01-01 00:00:00
abstract::The hallmarks of pulmonary Mycobacterium tuberculosis infection are lung granulomas. These organized structures are composed of host immune cells whose purpose is to contain or clear infection, creating a complex hub of immune and bacterial cell activity, as well as limiting pathology in the lungs. Yet, given cellular...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00426-18
更新日期:2018-08-22 00:00:00
abstract::Hybridoma cell lines which produced monoclonal antibodies against cholera toxin were isolated. These cell lines were detected with an enzyme-linked immunosorbent assay screening procedure with purified cholera toxin or subunit A of cholera toxin. Seven cell lines were characterized with respect to their reactivity wit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.267-272.1982
更新日期:1982-10-01 00:00:00
abstract::The facultative intracellular bacterial pathogen Listeria monocytogenes induces severe fetal infection during pregnancy. Little is known about the molecular mechanisms allowing the maternofetal transmission of bacteria. In this work, we studied fetoplacental invasion by infecting mice with various mutants lacking viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01570-06
更新日期:2007-02-01 00:00:00
abstract::Inoculation of neonatal CD-1 mice by multiple routes with an amyocarditic temperature-sensitive (ts) mutant (ts 1) derived from a myocarditic parent variant of coxsackievirus B3 (CVB3(m)) resulted in approximately half of the neonates surviving to adolescence. Challenge of the ts 1 survivors with CVB3(m) did not induc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.851-864.1983
更新日期:1983-02-01 00:00:00
abstract::Our previous animal studies showed that maternal Porphyromonas gingivalis infection in a subcutaneous chamber is associated with hepatic and uterine translocation, as well as systemic induction of maternal inflammatory responses, both of which were associated with fetal growth restriction (FGR). However, P. gingivalis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5163-5168.2003
更新日期:2003-09-01 00:00:00
abstract::Previous studies have noted that the prevalence of certain gonococcal colony types is influenced by the physiological state of the human host. Consequently eight different gonococcal strains were passed on clear typing medium, and opaque (Op) and transparent (Tr) variants of heavily pilated (P++) bacteria were selecte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.359-364.1978
更新日期:1978-11-01 00:00:00
abstract::A large body of evidence has convincingly shown that Toll-like receptors are necessary sensors for infections with pathogens, but their activation was also suggested to generate autoimmunity. During experimental infections, the lack of these sensors or of their signaling molecules should lead to a deficient immune res...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00951-07
更新日期:2008-04-01 00:00:00
abstract::Bordetella bronchiseptica is closely related to Bordetella pertussis, which produces respiratory disease primarily in mammals other than humans. However, its importance as a human pathogen is being increasingly recognized. Although a large amount of research on Bordetella has been generated regarding protein virulence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.1791-1798.2002
更新日期:2002-04-01 00:00:00
abstract::Hamster (Mesocricetus auratus) neutrophil granules contain at least four microbicidal peptides belonging to the defensin family. These compounds were purified from granule acid extracts by reverse-phase chromatography and termed HaNP-1 to -4 (hamster neutrophil peptide). HaNP-1 and HaNP-3 revealed the most bactericida...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4444-4449.1996
更新日期:1996-11-01 00:00:00
abstract::We have focused on regions of staphylococcal enterotoxin C1 (SEC1) causing immunomodulation. N-terminal deletion mutants lacking residues 6 through 13 induced T-cell proliferation similar to that induced by native toxin. However, mutants with residues deleted between positions 19 and 33, although nonmitogenic themselv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3396-3407.1994
更新日期:1994-08-01 00:00:00
abstract::Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The bacterium Neisseria meningitidis is the causative agent of meningitis and sepsis. A generally effective vaccine against N. meningitidis serogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion of N. meningitidis wild-ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00846-07
更新日期:2007-12-01 00:00:00
abstract::A family of high-molecular-weight (HMW) surface-exposed proteins important in the attachment of nontypeable Haemophilus influenzae (NTHi) to human epithelial cells was previously identified (J. W. St. Geme III, S. Falkow, and S. J. Barenkamp, Proc. Natl. Acad. Sci. USA 90:2875-2879, 1993). In the present investigation...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4460-4468.1994
更新日期:1994-10-01 00:00:00
abstract::Viral hepatitis caused by infection with murine hepatitis virus strain A59 has been shown to affect the mitotic response after partial hepatectomy in mice. Normal mice showed a delayed response, whereas nude mice did not show any response, even 5 days after partial hepatectomy. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.641-642.1981
更新日期:1981-08-01 00:00:00
abstract::The yield of infectious Chlamydia trachomatis was analyzed in human (HeLa) and mouse (McCoy) cell lines treated with the human interferon (IFN) subtypes IFN-alpha A and IFN-alpha D, with their hybrids [IFN-alpha AD (BglII), IFN-alpha AD (PvuII), and IFN-alpha DA (BglII)] constructed in vitro from their expression plas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.719-722.1985
更新日期:1985-03-01 00:00:00
abstract::The American Heart Association supports an association between periodontal diseases and atherosclerosis but not a causal association. This study explores the use of the integrin β6(-/-) mouse model to study the causality. We investigated the ability of a polymicrobial consortium of Porphyromonas gingivalis, Treponema ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01077-15
更新日期:2015-12-01 00:00:00
abstract::Forty-seven bacterial strains representing four different group C streptococcal species were tested for binding of human and bovine immunoglobulin G (IgG) subclasses. Specific binding sites for IgG were found in all bacterial species studied. The four species included differed, however, in their capacities to interact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.808-816.1980
更新日期:1980-03-01 00:00:00
abstract::Osteoarticular brucellosis is the most common complication in Brucella-infected humans regardless of age, sex, or immune status. The mechanism of bone destruction caused by Brucella species remained partially unknown due to the lack of a suitable animal model. Here, to study this complication, we explored the suitabil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00901-18
更新日期:2019-05-21 00:00:00
abstract::Macrophages can undergo apoptosis after infection with Mycobacterium tuberculosis. This macrophage response deprives the bacillus of its niche cell and supports the host response through better antigen presentation. The intracellular pathways of apoptosis that elaborate this macrophage response are not well understood...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01107-06
更新日期:2007-04-01 00:00:00
abstract::Saliva-binding molecules of Streptococcus sanguis and their receptors were investigated. Streptococcal cell surfaces were extracted with a barbital buffer and examined immunochemically. Strains G9B and Blackburn, which adhere specifically to saliva-coated hydroxyapatite via immunologically related adhesins, possess 80...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.64-70.1988
更新日期:1988-01-01 00:00:00
abstract::Listeria monocytogenes is an intracellular bacterial pathogen which causes bacteremia and has a tropism for the central nervous system and a propensity to cause maternofetal infection. L. monocytogenes has been shown to be an effective prophylactic and a therapeutic vaccine vector for viral and tumor antigens in anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3592-3601.2002
更新日期:2002-07-01 00:00:00
abstract::Streptococcus sanguis 804 (NCTC 10904) was grown ih batch culture at constant pH. and the glucosyltransferase activity of the supernatant was assayed over a 40-h growth period. The optimum pH for enzyme production was 7.0 to 7.2. During growth of the culture, three reproducible phases of enzyme activity were observed....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.50-58.1977
更新日期:1977-01-01 00:00:00
abstract::Intact tissues are relatively resistant to Pseudomonas aeruginosa-induced disease, and injury predisposes tissue to infection. Intact epithelia contain polarized cells that have distinct apical and basolateral membranes with unique lipids and proteins. In this study, the role of cell polarity in epithelial cell suscep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2861-2867.1997
更新日期:1997-07-01 00:00:00