Abstract:
:Intact tissues are relatively resistant to Pseudomonas aeruginosa-induced disease, and injury predisposes tissue to infection. Intact epithelia contain polarized cells that have distinct apical and basolateral membranes with unique lipids and proteins. In this study, the role of cell polarity in epithelial cell susceptibility to P. aeruginosa virulence mechanisms was tested. Madin-Darby canine kidney (MDCK) cells, human corneal epithelial cells, and primary cultures of two different types of airway epithelial cells were grown on Transwell filters or in plastic tissue culture wells. P. aeruginosa invasion of cells was quantified by gentamicin survival assays with two isolates that invade epithelial cells (6294 and PAO1). Cytotoxic activity was assessed by trypan blue exclusion assays with two cytotoxic strains (6206 and PA103). Basolateral surfaces of cells were exposed by one of two methods: EGTA pretreatment of epithelial cells or growth of cells in low-calcium medium. Both methods of exposing basolateral membranes increased epithelial cell susceptibility to P. aeruginosa invasion and cytotoxicity. Migrating cells were also found to be more susceptible to P. aeruginosa invasion than confluent monolayers that had established membrane polarity. Monolayers of MDCK cells that had been selected for resistance to killing by concanavalin A were resistant to both cytotoxicity and invasion by P. aeruginosa because they were more efficiently polarized for their susceptibility to P. aeruginosa virulence factors than regular MDCK cells and not because they were defective in glycosylation. These results suggest that there are factors on the basolateral surfaces of epithelial cells that promote interaction with P. aeruginosa or that there are inhibitory factors on the apical cell surface. Thus, cell polarity of intact epithelia is likely to contribute to defense against P. aeruginosa infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Fleiszig SM,Evans DJ,Do N,Vallas V,Shin S,Mostov KEdoi
10.1128/IAI.65.7.2861-2867.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
2861-7issue
7eissn
0019-9567issn
1098-5522journal_volume
65pub_type
杂志文章abstract::Group B streptococci (GBS) adhere to surface receptors present on epithelial cells; these receptors include fibronectin and laminin. To identify other possible receptors, plasma membranes from A549 cells, a respiratory tract epithelial cell line, were prepared. These plasma membranes were tested in a protein blot anal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2129-2134.2000
更新日期:2000-04-01 00:00:00
abstract::Theileria parasites infect and transform bovine leukocytes. We have analyzed laboratory-established Theileria sp.-infected leukocyte lines and observed that transformed macrophages express CD5. Low-level expression of CD5 by macrophages was further confirmed on three independent Theileria annulata clinical isolates fr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Tuberculosis continues to ravage humanity, killing 2 million people yearly. Most cases occur in areas of the world to which the disease is endemic, where almost everyone is vaccinated early in life with Mycobacterium bovis BCG, the currently available vaccine against tuberculosis. Thus, while more-potent vaccines are ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4676-4683.2005
更新日期:2005-08-01 00:00:00
abstract::Once Candida albicans comes in contact with endothelial cells, it induces cellular injury. This endothelial cell injury may be a mechanism by which blood-borne organisms escape from the intravascular compartment and invade the tissue parenchyma during hematogenous infection. We have been investigating the ability of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4714-4718.1996
更新日期:1996-11-01 00:00:00
abstract::Continuous virus production is a characteristic of chicken embryo fibroblasts (CEF) infected and transformed by a nondefective Schmidt-Ruppin subgroup A Rous sarcoma virus. This virus production has been examined with particular attention to the amount of newly budded virus which remained cell-associated, and to the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.4.834-843.1974
更新日期:1974-10-01 00:00:00
abstract::Vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. Gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01305-15
更新日期:2016-06-23 00:00:00
abstract::Brucella abortus and polyacrylic acid, two agents whose interferon-inducing capacity in the mouse has been well established, failed to stimulate circulating interferon production in rabbits, yet protected rabbits against local vaccinia virus infection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.669-673.1973
更新日期:1973-10-01 00:00:00
abstract::Curli are thin, coiled fibers expressed on the surface of Escherichia coli that bind several matrix and plasma proteins such as fibronectin, laminin, plasminogen, tissue plasminogen activator, and H-kininogen. In this work, we examined the interactions between curli-expressing E. coli and human major histocompatibilit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.3.944-949.1998
更新日期:1998-03-01 00:00:00
abstract::A hemolysin that appears to be responsible for the zones of beta-hemolysis surrounding colonies of group B streptococci (Streptococcus agalactiae) on blood agar plates has been isolated and partially purified. No soluble hemolysin was detectable in the supernatants of streptococcal cultures grown in several types of m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The outer membrane (OM) of Gram-negative bacteria is an asymmetric lipid bilayer that consists of inner leaflet phospholipids and outer leaflet lipopolysaccharides (LPS). The asymmetric character and unique biochemistry of LPS molecules contribute to the OM's ability to function as a molecular permeability barrier tha...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00920-19
更新日期:2020-06-22 00:00:00
abstract::Mice with disrupted beta 2-microglobulin (beta 2m-/-), I-A (class II-/-), or CD4 (CD4-/-) genes were examined for their capacity to resolve Chlamydia trachomatis genital tract infection. C57BL/6 and beta 2m-/- mice resolved infection similarly and were culture negative by 4 to 5 weeks following infection. Conversely, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4661-4668.1995
更新日期:1995-12-01 00:00:00
abstract::Incubation of large granular lymphocytes (LGL) with glutaraldehyde-fixed bacteria stimulated in the supernatant the production of interferon (IFN), which proved to be mainly IFN-gamma. Even though IFN-gamma was produced upon exposure of LGL to bacteria, anti-IFN-gamma antibodies failed to interfere with induction of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2767-2773.1991
更新日期:1991-08-01 00:00:00
abstract::Using a murine respiratory infection model, we have demonstrated previously that infection with Bordetella pertussis or immunization with a whole-cell pertussis vaccine induced antigen-specific Th1 cells, which conferred a high level of protection against aerosol challenge. In contrast, immunization with an acellular ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5295-5301.1996
更新日期:1996-12-01 00:00:00
abstract::Factors in the queous humor capable of increasing the random migration and chemotaxis of monocytes were demonstrated during experimental uveitis in the rabbit. Injection of endotoxin-free human serum albumin into the vitreous produced a severe inflammation detectable after 7 days and maximal between 12 to 21 days. Aqu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1110-1116.1976
更新日期:1976-04-01 00:00:00
abstract::Oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with Campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. Disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. Orally infected animals developed agglutinin titers of 8 to 256 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1848-1852.1990
更新日期:1990-06-01 00:00:00
abstract::We have previously shown that a pneumococcal surface protein A (PspA)-based vaccine containing DNA plasmid encoding the Flt3 ligand (FL) gene (pFL) as a nasal adjuvant prevented nasal carriage of Streptococcus pneumoniae. In this study, we further investigated the safety and efficacy of this nasal vaccine for the indu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01360-10
更新日期:2011-07-01 00:00:00
abstract::Natural killer (NK) cells are critically involved in the early immune response against various intracellular pathogens, including Coxiella burnetii and Chlamydia psittaciChlamydia-infected NK cells functionally mature, induce cellular immunity, and protect themselves by killing the bacteria in secreted granules. Here,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00172-20
更新日期:2020-10-19 00:00:00
abstract::The ability of human clinical isolates of Yersinia enterocolitica to interact with HEp-2 cells was found to vary considerably between the serotypes O:3, O:8, and O:9. Although all three serotypes adhered initially to the cell surface, regardless of incubation temperature of the bacterial inoculum or presence of the 40...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.85-89.1986
更新日期:1986-04-01 00:00:00
abstract::Invasive fungal infections represent an increasing problem associated with high mortality. The present study was undertaken to identify leukocyte subsets that are activated by hyphal fragments in a whole-human-blood model, as well as to examine the involvement of CD14 and Toll-like receptors (TLRs) in activation of mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2402-2406.2001
更新日期:2001-04-01 00:00:00
abstract::Sequestration of Plasmodium falciparum-infected erythrocytes to the capillary endothelium can cause obstruction and localized tissue damage. Occlusion of vessels in falciparum malaria infection has been related to two properties of the parasite: adhesion to endothelial cells and rosette formation. Our study on P. falc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4483-4490.1992
更新日期:1992-11-01 00:00:00
abstract::Borrelia burgdorferi, a causative agent of Lyme borreliosis, is a zoonotic pathogen that survives in nutrient-limited environments within a tick, prior to transmission to its mammalian host. Survival under these prolonged nutrient-limited conditions is thought to be similar to survival during stationary phase, which i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00925-15
更新日期:2015-12-01 00:00:00
abstract::The mechanism by which recombinant murine gamma interferon (rMuIFN-gamma) and bacterial lipopolysaccharide (LPS) activate mouse resident splenic macrophages to inhibit the intracellular growth of the fungus Histoplasma capsulatum was examined. Growth inhibition depended on L-arginine metabolism. The growth inhibitory ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1940-1945.1994
更新日期:1994-05-01 00:00:00
abstract::In this study, cytokine patterns produced by CD4(+) T cells isolated from antrum or corpus gastral biopsy specimens of 10 patients with Helicobacter pylori-positive gastritis were compared. To this end, expression of intracellular cytokines (interleukin-4 [IL-4] and gamma interferon) and of CD4 was assessed by flow cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5543-5546.1998
更新日期:1998-11-01 00:00:00
abstract::Investigating the innate immune response mediators released in milk has manifold implications, spanning from elucidation of the role played by mammary epithelial cells (MECs) in fighting microbial infections to the discovery of novel diagnostic markers for monitoring udder health in dairy animals. Here, we investigate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-13
更新日期:2013-09-01 00:00:00
abstract::Three antigenic determinants were revealed in H3 hemagglutinin of influenza A viruses isolated from 1968 to 1975. One of them was common for all viruses, and two others specified differences between the viruses possessing H3 hemagglutinin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.804-807.1979
更新日期:1979-06-01 00:00:00
abstract::Escherichia coli heat-labile enterotoxin (LT) is an extensively studied adjuvant of mucosal responses. Nevertheless, its mode of action as an adjuvant remains incompletely understood. In this study, we describe a simplified in vitro model with which to look at some aspects of immunoregulation by LT. The interaction of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6891-6895.2000
更新日期:2000-12-01 00:00:00
abstract::Cryptosporidial infections in severe combined immune deficient (SCID) mice produce a chronic disease state which in the later stages leads to extraintestinal involvement and hepatic dysfunction. To further characterize the infection dynamics in this model and monitor the changes in the hepatic system, a dose titration...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1691-1695.1994
更新日期:1994-05-01 00:00:00
abstract::Endotoxin-resistant C3H/HeJ mice were used to test the hypothesis that a macrophage product, possibly interleukin-1, might mediate the depression of liver cytochrome P-450-dependent drug metabolism in endotoxin-treated mice. Depression of liver drug metabolism by endotoxin was observed in normal mice (C3H/HeN) but not...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.837-840.1986
更新日期:1986-12-01 00:00:00
abstract::Lipopolysaccharide (LPS) has recently been shown to facilitate macrophage foam cell formation and has been suggested to be a proatherogenic factor. The mechanism of LPS induced cholesterol accumulation, however, is unclear. In this report, using the macrophage-like RAW 264.7 cell line, we provide experimental evidence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2995-3003.2002
更新日期:2002-06-01 00:00:00
abstract::Antibodies against the Plasmodium falciparum P0 ribosomal phosphoprotein (PfP0) have been detected exclusively but extensively in malaria-immune persons. Polyclonal rabbit and mice sera were raised against two recombinant polypeptides of P. falciparum P0 protein, PfP0N and PfP0C, covering amino acids 17 to 61 and the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00