Abstract:
:The mechanism by which recombinant murine gamma interferon (rMuIFN-gamma) and bacterial lipopolysaccharide (LPS) activate mouse resident splenic macrophages to inhibit the intracellular growth of the fungus Histoplasma capsulatum was examined. Growth inhibition depended on L-arginine metabolism. The growth inhibitory state normally induced by rMuIFN-gamma and LPS in resident splenic macrophages did not occur when the macrophages were cultured in the presence of NG-monomethyl-L-arginine, a competitive inhibitor of L-arginine metabolism. Resident splenic macrophages treated with rMuIFN-gamma and LPS produced nitrite (NO2-), an end product of L-arginine metabolism. When macrophages were cultured in the presence of NG-monomethyl-L-arginine together with rMuIFN-gamma and LPS, only baseline levels of NO2- were detected. Spleen cells from H. capsulatum-infected mice produced high levels of NO2- in culture. The production of NO2- correlated with in vitro inhibition of the intracellular growth of H. capsulatum. Anti-tumor necrosis factor alpha antibody did not block NO2- production by the immigrant splenic macrophages and did not abolish the antihistoplasma activity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lane TE,Wu-Hsieh BA,Howard DHdoi
10.1128/IAI.62.5.1940-1945.1994subject
Has Abstractpub_date
1994-05-01 00:00:00pages
1940-5issue
5eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Antisera were produced by inoculation of mycoplasma cells grown in PPLO broth supplemented with rabbit serum alone or rabbit serum plus Freund complete or incomplete adjuvant. By using an enzyme-linked immunosorbent assay, immunoglobulin G antibodies to cholesterol were detected in antisera to mycoplasmas (Mycoplasma ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.2200-2202.1991
更新日期:1991-06-01 00:00:00
abstract::Neisseria gonorrhoeae is an obligate human pathogen that causes the common sexually transmitted infection gonorrhea. Gonococcal infections cause significant morbidity, particularly among women, as the organism ascends to the upper reproductive tract, resulting in pelvic inflammatory disease, ectopic pregnancy, and inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05806-11
更新日期:2011-12-01 00:00:00
abstract::Toxin B from Clostridium difficile is cytopathic in vitro for various types of cells, including polymorphonuclear cells, lymphocytes, and monocytes. Since intestine lamina propria is rich in macrophages, we studied the effect of toxin B on human monocytes and on human macrophages generated in vitro by long-term cultur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.1082-1090.1993
更新日期:1993-03-01 00:00:00
abstract::Recent reports have demonstrated that Bordetella pertussis has invasive behavior in vivo and in vitro. In this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants deficient in specific virulence factors to enter and survive intracellularly in human macrophages in vitro. Uptake of vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4578-4585.1992
更新日期:1992-11-01 00:00:00
abstract::Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2285-2288.1994
更新日期:1994-06-01 00:00:00
abstract::Photobacterium damselae subsp. damselae causes infections and fatal disease in marine animals and in humans. Highly hemolytic strains produce damselysin (Dly) and plasmid-encoded HlyA (HlyA(pl)). These hemolysins are encoded by plasmid pPHDD1 and contribute to hemolysis and virulence for fish and mice. In this study, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00155-13
更新日期:2013-09-01 00:00:00
abstract::While investigating the virulence traits of Staphylococcus aureus adhering to the skin of atopic-dermatitis (AD) patients, we identified a novel open reading frame (ORF) with structural similarity to a superantigen from genome sequence data of an isolate from AD skin. Concurrently, the same ORF was identified in a bov...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00360-19
更新日期:2020-01-22 00:00:00
abstract::Pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. P. multocida strains simultaneously express two lipopolysaccharide (LPS) glycoforms (glycoforms A and B) that differ only in their inner core structure. Glycoform A contains a single 3-deoxy-d-manno-octulosonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00457-10
更新日期:2010-09-01 00:00:00
abstract::The mechanism of adsorption of the Streptococcus mutans enzymes responsible for the synthesis of insoluble dextran-levan to the S. mutans cell-wall binding sites has been studied. Certain characteristics of these binding sites are presented. The adsorption of these enzymes to the cell surface occurred rapidly without ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.419-429.1974
更新日期:1974-02-01 00:00:00
abstract::Borrelia burgdorferi, a causative agent of Lyme borreliosis, is a zoonotic pathogen that survives in nutrient-limited environments within a tick, prior to transmission to its mammalian host. Survival under these prolonged nutrient-limited conditions is thought to be similar to survival during stationary phase, which i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00925-15
更新日期:2015-12-01 00:00:00
abstract::Apical membrane antigen 1 (AMA1) is expressed on the surfaces of Plasmodium falciparum merozoites and is thought to play an important role in the invasion of erythrocytes by malaria parasites. To select for peptides that mimic conformational B-cell epitopes on AMA1, we screened a phage display library of >10(8) indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1126-1134.2004
更新日期:2004-02-01 00:00:00
abstract::Several early nonspecific host defense mechanisms were examined in resistant (BALB/c) and susceptible (C3H/He) mice after intraperitoneal inoculation with Rickettsia tsutsugamushi strain Gilliam. Inflammatory exudates were formed in both mouse strains in response to rickettsial inoculation, but the inflammatory respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1239-1250.1981
更新日期:1981-03-01 00:00:00
abstract::The blood-stage development of malaria parasites is initiated by the invasion of merozoites into susceptible erythrocytes. Specific receptor-ligand interactions must occur for the merozoites to first attach to and then invade erythrocytes. Because the invasion process is essential for the parasite's survival and the m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5581-5586.1994
更新日期:1994-12-01 00:00:00
abstract::Vaccine antigen-specific antibody-secreting cell (ASC) responses in peripheral blood of healthy adult volunteers were studied after oral immunization with a prototype enterotoxigenic Escherichia coli (ETEC) vaccine by means of the enzyme-linked immunospot technique. Three doses of vaccine consisting of formalin-killed...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2605-2611.1992
更新日期:1992-07-01 00:00:00
abstract::Dentilisin is a major surface protease and virulence factor of the bacterium Treponema denticola. In this study, we found that T. denticola reduced inflammatory cytokines, including interleukin-1beta (IL-1beta), IL-6, and tumor necrosis factor alpha, in peripheral blood mononuclear cells through degradation by dentili...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2462-2467.2006
更新日期:2006-04-01 00:00:00
abstract::We demonstrate that 2-month-old female B10.T(6R) mice are highly resistant to systemic infection with the KIM5 strain of Yersinia pestis and that B10.T(6R) mice become susceptible to Y. pestis infection by the age of 5 months. In this study, young (2-month-old) and middle-aged (5- to 12-month-old) B10.T(6R) mice were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05267-11
更新日期:2011-11-01 00:00:00
abstract::Some interrelationships among age, ambient temperature, intestinal transit, and enterotoxigenic Escherichia coli infection were studied in an infant mouse model. The transit of dye in the small intestine was accelerated during the response to heat-stable E. coli enterotoxin. Transit in the small intestine of normal mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.127-132.1979
更新日期:1979-07-01 00:00:00
abstract::Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination of inhaled Cryptococcus neoformans from the lung to the brain. The mechanism(s) by which C. neoformans crosses the blood-brain barrier (BBB) is a key unresolved issue in cryptococcosis. We used both an in vivo mouse model and an in vit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.4985-4995.2004
更新日期:2004-09-01 00:00:00
abstract::Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, eae-positive STEC strains, particularly those belonging to serogroups O157 and O111, appear to have greater virulence for humans. However, in spite of b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5930-5937.1999
更新日期:1999-11-01 00:00:00
abstract::Characteristics of four transmissible gastroenteritis (TGE) virus field strains (Miller, Purdue, Bl, and V203) and four cell culture-attenuated strains (Purdue, SH, CKp, and Bl) were studied to find methods of differentiation between the two groups of viruses. TGE field virus strains did not replicate as well as atten...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1642-1646.1976
更新日期:1976-06-01 00:00:00
abstract::Porphyromonas gingivalis forms communities with antecedent oral biofilm constituent streptococci. P. gingivalis major fimbriae bind to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) present on the streptococcal surface, and this interaction plays an important role in P. gingivalis colonization. This study identified...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00439-09
更新日期:2009-11-01 00:00:00
abstract::Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2408-2411.2004
更新日期:2004-04-01 00:00:00
abstract::Black melanin-like pigments are produced by several neurotropic fungi, including Cryptococcus neoformans. Pigment production is associated with virulence. In media containing phenolic substrates such as L-dopa, C. neoformans cells become black as a result of pigment accumulation. Pigmented and nonpigmented C. neoforma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3131-3136.1995
更新日期:1995-08-01 00:00:00
abstract::Ers has been qualified as the PrfA-like transcriptional regulator of Enterococcus faecalis. In a previous study we reported that Ers is important for the survival within macrophages of this opportunist pathogenic bacterium. In the present work we have used proteomic and microarray expression profiling of E. faecalis J...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00161-08
更新日期:2008-07-01 00:00:00
abstract::Naturally occurring antibodies for Vibrio fetus with whole-cell and O antigen specificities were detected in sera of mature cattle by means of the agglutination and indirect immunofluorescence reactions. O antibodies occurred in each of 21 sera examined, and whole-cell antibodies, in lower concentrations, occurred in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.728-733.1972
更新日期:1972-05-01 00:00:00
abstract::Human gingival fibroblasts were exposed in culture to cell extracts of different black-pigmented Bacteroides species, and their growth was monitored by determining thymidine uptake and counting cells. Of the Bacteroides species tested (B. gingivalis, B. asaccharolyticus, and B. intermedius), B. gingivalis gave the ext...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.201-205.1987
更新日期:1987-01-01 00:00:00
abstract::Two virulence factors of Listeria monocytogenes, listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC), mediate escape of this pathogen from the phagocytic vacuole of macrophages, thereby allowing the bacterium access to the host cell cytosol for growth and spread to neighboring cells. We ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6639-6646.2005
更新日期:2005-10-01 00:00:00
abstract::Neisseria meningitidis is a major cause of sepsis and meningitis but is also a common commensal, present in the nasopharynx of between 8 and 20% of healthy individuals. During carriage, the bacterium is found on the surface of the nasopharyngeal epithelium and in deeper tissues, while to develop disease the meningococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01377-09
更新日期:2010-09-01 00:00:00
abstract::Broth culture supernatants from Tox+ Helicobacter pylori strains induce vacuolation of HeLa cells in vitro and contain VacA in concentrations that are higher than those found in supernatants from Tox- H. pylori strains. To investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cyto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3088-3094.1998
更新日期:1998-07-01 00:00:00
abstract::Infection of epithelial cells by two biovars of Chlamydia trachomatis results in the tyrosine phosphorylation of several host proteins. The most prominent change in host protein tyrosine phosphorylation involves a complex of proteins with molecular masses of 75 to 85 kDa (pp75/85) and 100 kDa (pp100). The C. trachomat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5301-5308.1997
更新日期:1997-12-01 00:00:00