Abstract:
:The HT29MTXE12 (E12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterized the interaction of Campylobacter jejuni with the E12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate C. jejuni virulence properties. C. jejuni 81-176 colonized and reproduced in E12 mucus. Adhesion to and internalization of C. jejuni were enhanced in E12 cells harboring mucus compared to parental cells without mucus. Translocation of C. jejuni occurred at early time points following infection. C. jejuni aligned with tight junctions and colocalized with the tight junction protein occludin, suggesting a paracellular route of translocation. Probiotic strains Lactobacillus rhamnosus R0011, Lactobacillus helveticus R0052, Lactobacillus salivarius AH102, Bifidobacterium longum AH1205, a commercial combination of L. rhamnosus R0011 and L. helveticus R0052 (Lacidofil), and a cocktail consisting of L. rhamnosus, L. helveticus, and L. salivarius (RhHeSa) colonized E12 mucus and bound to underlying cells. Probiotics attenuated C. jejuni association with and internalization into E12 cells and translocation to the basolateral medium of transwells. Live bacteria and prolonged precolonization of E12 cells with probiotics were necessary for probiotic action. These results demonstrate the potential for E12 cells as a model of mucosal pathogenesis and provide a rationale for the further investigation of probiotics as prophylaxis against human campylobacteriosis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Alemka A,Clyne M,Shanahan F,Tompkins T,Corcionivoschi N,Bourke Bdoi
10.1128/IAI.01249-09subject
Has Abstractpub_date
2010-06-01 00:00:00pages
2812-22issue
6eissn
0019-9567issn
1098-5522pii
IAI.01249-09journal_volume
78pub_type
杂志文章abstract::Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from hors...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2776-2782.1999
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journal_title:Infection and immunity
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doi:10.1128/IAI.26.1.15-18.1979
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.554-559.1981
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.41.2.563-569.1983
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3451-3462.1991
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.3006-3011.2006
更新日期:2006-05-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.10.2538-2543.1988
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.757-765.1981
更新日期:1981-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.4.834-843.1974
更新日期:1974-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2003-2010.1993
更新日期:1993-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.728-731.1978
更新日期:1978-06-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.45.2.518-521.1984
更新日期:1984-08-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.64.4.1351-1356.1996
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1860-1866.1994
更新日期:1994-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.229-239.1982
更新日期:1982-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1658-1660.1988
更新日期:1988-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.34-40.1974
更新日期:1974-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.764-775.1991
更新日期:1991-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.827-835.1978
更新日期:1978-06-01 00:00:00