Abstract:
:Trypanosoma cruzi species is categorized into six discrete typing units (TcI to TcVI) of which TcI is most abundantly noted in the sylvatic transmission cycle and considered the major cause of human disease. In our study, the TcI strains Colombiana (COL), SylvioX10/4 (SYL), and a cultured clone (TCC) exhibited different biological behavior in a murine model, ranging from high parasitemia and symptomatic cardiomyopathy (SYL), mild parasitemia and high tissue tropism (COL), to no pathogenicity (TCC). Proteomic profiling of the insect (epimastigote) and infective (trypomastigote) forms by two-dimensional gel electrophoresis/matrix-assisted laser desorption ionization-time of flight mass spectrometry, followed by functional annotation of the differential proteome data sets (≥2-fold change, P < 0.05), showed that several proteins involved in (i) cytoskeletal assembly and remodeling, essential for flagellar wave frequency and amplitude and forward motility of the parasite, and (ii) the parasite-specific antioxidant network were enhanced in COL and SYL (versus TCC) trypomastigotes. Western blotting confirmed the enhanced protein levels of cytosolic and mitochondrial tryparedoxin peroxidases and their substrate (tryparedoxin) and iron superoxide dismutase in COL and SYL (versus TCC) trypomastigotes. Further, COL and SYL (but not TCC) were resistant to exogenous treatment with stable oxidants (H2O2 and peroxynitrite [ONOO(-)]) and dampened the intracellular superoxide and nitric oxide response in macrophages, and thus these isolates escaped from macrophages. Our findings suggest that protein expression conducive to increase in motility and control of macrophage-derived free radicals provides survival and persistence benefits to TcI isolates of T. cruzi.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Zago MP,Hosakote YM,Koo SJ,Dhiman M,Piñeyro MD,Parodi-Talice A,Basombrio MA,Robello C,Garg NJdoi
10.1128/IAI.00193-16subject
Has Abstractpub_date
2016-05-24 00:00:00pages
1842-1856issue
6eissn
0019-9567issn
1098-5522pii
IAI.00193-16journal_volume
84pub_type
杂志文章abstract::Ten cultures of Mycobacterium tuberculosis, one of Mycobacterium kansasii (nonsignificant), and one of Mycobacterium phlei were submitted to starvation. As a result they lost first their acid fastness and then all other staining affinities but, in this chromophobic state, they survived for at least 2 years and, after ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.5.843-850.1974
更新日期:1974-05-01 00:00:00
abstract::We have determined the nucleotide sequence of the 1.4-kilobase DNA fragment containing the plasminogen activator gene (pla) of Yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. The sequence revealed the presence of a 936-base-pair open reading frame that constitutes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1517-1523.1989
更新日期:1989-05-01 00:00:00
abstract::The ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent Rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (Microtus pennsylvanicus) was studied by transmission electron microscopy. In sections of epoxy-embedded specimens stained with u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1252-1260.1983
更新日期:1983-09-01 00:00:00
abstract::To determine whether lipopolysaccharide (LPS) structures of Campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the LPS of these strains with immune rabbit serum raised against six Campylobacter jejuni strains and two Campylobacter f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.204-208.1986
更新日期:1986-01-01 00:00:00
abstract::Specific pathogen-free guinea pigs were infected via the respiratory route with viable, attenuated Mycobacterium tuberculosis H37Ra and maintained on purified isocaloric diets. The control diet contained 30% protein (ovalbumin) and 50 ppm of added zinc (50 micrograms/g), the low protein diet contained 10% protein and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.793-799.1983
更新日期:1983-02-01 00:00:00
abstract::The goal of this study was to determine the roles of eosinophils and neutrophils in innate and adaptive protective immunity to larval Strongyloides stercoralis in mice. The experimental approach used was to treat mice with an anti-CCR3 monoclonal antibody to eliminate eosinophils or to use CXCR2-/- mice, which have a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01958-05
更新日期:2006-10-01 00:00:00
abstract::We compared the susceptibilities of Chlamydia trachomatis elementary bodies (EBs) to human defensin HNP-2 and porcine protegrin PG-1, cysteine-rich beta-sheet antimicrobial peptides produced by mammalian leukocytes. Although both peptides protected McCoy cell monolayers from infection by chlamydial EBs, protegrins wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.709-713.1996
更新日期:1996-03-01 00:00:00
abstract::We have previously reported that a wild strain of Francisella tularensis is much less efficiently killed by human polymorphonuclear leukocytes than is an attenuated strain. In the present study, the killing of the attenuated strain was found to be strictly oxygen dependent. The wild and the attenuated strains both ind...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.730-734.1984
更新日期:1984-02-01 00:00:00
abstract::In attempts to reactivate latent herpes simplex virus, we instilled Diplococcus pneumoniae intratracheally into mice harboring latent infections in sacrosciatic spinal ganglia. All mice developed a severe pneumonia within 24 h and were given penicillin therapy. Representative mice that survived the penumonia were sacr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.635-639.1975
更新日期:1975-04-01 00:00:00
abstract::To analyze the role of interleukin-10 (IL-10) in bacterial cerebral infections, we studied cerebral listeriosis in IL-10-deficient (IL-10(-/-)) and wild-type (WT) mice, the latter of which express high levels of IL-10 in both primary and secondary cerebral listeriosis. IL-10(-/-) mice succumbed to primary as well as s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4561-4571.2001
更新日期:2001-07-01 00:00:00
abstract::Viral hepatitis caused by infection with murine hepatitis virus strain A59 has been shown to affect the mitotic response after partial hepatectomy in mice. Normal mice showed a delayed response, whereas nude mice did not show any response, even 5 days after partial hepatectomy. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.641-642.1981
更新日期:1981-08-01 00:00:00
abstract::Fourteen strains of Escherichia coli were tested for ability to cause infective endocarditis in rabbits prepared by prior placement of an intracardiac catheter. Strains that were resistant to the bactericidal action of serum caused E. coli endocarditis in 91.4% of rabbits, whereas serum-sensitive strains usually faile...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.213-217.1977
更新日期:1977-04-01 00:00:00
abstract::Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00139-16
更新日期:2016-06-23 00:00:00
abstract::The etiology of recurrent vulvovaginal candidiasis in otherwise healthy women of child-bearing age remains an enigma. To date, results from both clinical studies and a murine model of vaginal candidiasis indicate that Candida vaginitis can occur in the presence of Candida-specific Th1-type cell-mediated immunity expre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2403-2408.1995
更新日期:1995-07-01 00:00:00
abstract::Gardnerella vaginalis, the bacterial species most frequently isolated from women with bacterial vaginosis (BV), produces a cholesterol-dependent cytolysin (CDC), vaginolysin (VLY). At sublytic concentrations, CDCs may initiate complex signaling cascades crucial to target cell survival. Using live-cell imaging, we obse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00627-13
更新日期:2013-12-01 00:00:00
abstract::Campylobacter jejuni is a microaerobic bacterium that produces an acute, self-limiting, watery or bloody diarrhea in humans. Little is known about how C. jejuni causes disease or even what specific capabilities it requires for survival in vivo. The enzyme, superoxide dismutase (SOD), which catalyzes the breakdown of s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2687-2694.1994
更新日期:1994-07-01 00:00:00
abstract::Pneumonia due to Gram-negative bacteria is associated with high mortality. Acinetobacter baumannii is a Gram-negative bacterium that is associated with hospital-acquired and ventilator-associated pneumonia. Bacteria have been described to release outer membrane vesicles (OMVs) that are capable of mediating systemic in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00243-19
更新日期:2019-08-21 00:00:00
abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3272-3279.1988
更新日期:1988-12-01 00:00:00
abstract::Thrombospondin-related anonymous protein (TRAP) of the malaria parasite Plasmodium falciparum shares two sequence motifs with other proteins which possess adhesive properties. Recently, findings indicate that TRAP is an antigen which contributes to antisporozoite immunity. We have cloned and expressed the TRAP coding ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3490-3495.1993
更新日期:1993-08-01 00:00:00
abstract::A ruthenium red-staining capsule was observed on two pathogenic strains, but not on one nonpathogenic strain of Mycoplasma gallisepticum. The capsule appeared to mediate cytadsorption of mycoplasmas to the chicken tracheal epithelium without evidence of membrane fusion. No relationship was seen between the presence of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.2.830-833.1982
更新日期:1982-05-01 00:00:00
abstract::Ehrlichia chaffeensis, an obligatory intracellular bacterium of monocytes or macrophages, is the etiologic agent of human monocytic ehrlichiosis. Our previous study showed that gamma interferon (IFN-gamma) added prior to or at early stage of infection inhibited infection of human monocytes with E. chaffeensis; however...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2514-2520.1998
更新日期:1998-06-01 00:00:00
abstract::Mycobacterium bovis BCG is widely used as a vaccine against tuberculosis (TB), despite its variable protective efficacy. Relatively little is known about the immune response profiles following BCG vaccination in relation to protection against TB. Here we tested whether BCG vaccination results in immune responses to Do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01999-06
更新日期:2007-07-01 00:00:00
abstract::Rocky Mountain spotted fever, a systemic tick-borne illness caused by the obligate intracellular bacterium Rickettsia rickettsii, is associated with widespread infection of the vascular endothelium. R. rickettsii infection induces a biphasic pattern of the nuclear factor-kappaB (NF-kappaB) activation in cultured human...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.155-165.2005
更新日期:2005-01-01 00:00:00
abstract::A main feature in the pathogenesis of bacterial endocarditis is the activation of the coagulation system via the extrinsic pathway, resulting in the formation of infected endocardial vegetations. Earlier studies gave indirect evidence that monocytes play an important role in the procoagulant response during the course...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.448-451.1996
更新日期:1996-02-01 00:00:00
abstract::To identify potential vaccine candidates for the prevention of infection with the filarial nematode Onchocerca volvulus, we screened an O. volvulus L3 stage cDNA library with sera from putatively immune (PI) subjects, and a prominent immunogenic clone of 1,184 nucleotides was identified. It contained an open reading f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.851-858.2002
更新日期:2002-02-01 00:00:00
abstract::The involvement of tumor necrosis factor (TNF) in resistance to Toxoplasma gondii infection was examined by means of experiments in which mice were treated with anti-TNF antibodies prior to infection with ME49, a low-virulence Toxoplasma strain. In (BALB/cBy x C57BL/6J)F1 (CB6F1) mice, which are highly resistant to in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1979-1983.1992
更新日期:1992-05-01 00:00:00
abstract::Pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. P. multocida strains simultaneously express two lipopolysaccharide (LPS) glycoforms (glycoforms A and B) that differ only in their inner core structure. Glycoform A contains a single 3-deoxy-d-manno-octulosonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00457-10
更新日期:2010-09-01 00:00:00
abstract::Phase 1 and 2 clinical trials of group B streptococcal (GBS) capsular polysaccharide (CPS)-protein conjugate vaccines in healthy adults have demonstrated their safety and improved immunogenicity compared with uncoupled CPSs. Two recent trials sought to determine (i) whether adsorption of conjugate vaccine to aluminum ...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.69.11.6696-6701.2001
更新日期:2001-11-01 00:00:00
abstract::During the infection of mice with Listeria monocytogenes, there is a profound depletion of T (Thy-1+ Ig-) lymphocytes between days 1 and 4, followed by an increase in T cells to three times normal levels by day 9. The recovery of T cell numbers required cell proliferation, being sensitive to vinblastin and cyclophosph...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.694-698.1982
更新日期:1982-11-01 00:00:00
abstract::The appearance of lymphotoxin in the culture fluid of phytohemagglutinin (PHA)-stimulated mouse spleen cells required two cell fractions that were separated by adherence to plastic. Upon stimulation with PHA, neither cell fraction alone produced significant amounts of lymphotoxin; however, when the cell fractions were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.1028-1035.1982
更新日期:1982-06-01 00:00:00