Abstract:
:To determine whether lipopolysaccharide (LPS) structures of Campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the LPS of these strains with immune rabbit serum raised against six Campylobacter jejuni strains and two Campylobacter fetus strains. The LPS studied were from Salmonella minnesota wild type and Ra to Re mutants, Salmonella typhi, Escherichia coli, Yersinia enterocolitica, Vibrio cholerae, and Pseudomonas aeruginosa. None of the 11 LPS preparations was recognized by the eight antisera, but antisera to each of the Campylobacter strains recognized core determinants of some LPS preparations. Antiserum directed against the most serum-sensitive C. jejuni strain, 79-193, was the only antiserum sample that recognized core regions of the rough Salmonella mutants. In converse experiments, when LPS preparations from five Campylobacter strains were blotted with antiserum to Salmonella lipid A, recognition of core structures of each was shown; data from an enzyme-linked immunosorbent assay confirmed this result. In contrast, antiserum to Salmonella typhimurium Re LPS showed no reactivity. We conclude that LPS of Campylobacter strains share lipid A antigenic determinants with the core region of LPS of several other gram-negative organisms.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Perez-Perez GI,Hopkins JA,Blaser MJdoi
10.1128/IAI.51.1.204-208.1986subject
Has Abstractpub_date
1986-01-01 00:00:00pages
204-8issue
1eissn
0019-9567issn
1098-5522journal_volume
51pub_type
杂志文章abstract::Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.853-860.1979
更新日期:1979-12-01 00:00:00
abstract::Borrelia burgdorferi-induced arthritis in mice is characterized by tendonitis, synovitis, and inflammatory-cell infiltrate, predominantly of neutrophils. Because genetic deficiency in E and P selectins results in delayed recruitment of neutrophils to sites of inflammation, mice with this deficiency were tested for the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Whole blood and plasma from animals in the acute stage of leptospirosis contained a toxic factor which produced a cytopathic effect on fibroblastic L cell monolayers. Firm adsorption of cytotoxic factor (CTF) to L cells occurred within 1 h. The highest titer of CTF in plasma was reached at 24 h and declined after 48 h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.401-405.1973
更新日期:1973-09-01 00:00:00
abstract::A model of invasive Haemophilus influenzae type b disease in rats was used to compare the net in vivo and in vitro multiplication rates of this bacterium. In both the blood of rats and in vitro cultures (fresh rat blood or enriched broth) there was an exponential increase in the number of CFU per milliliter with calcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.911-913.1986
更新日期:1986-06-01 00:00:00
abstract::Outer surface protein A (OspA) of the Lyme disease spirochete is primarily produced in the tick vector. OspA, which is a receptor for attaching spirochetes to the tick gut, is down regulated as the spirochetes leave the tick and enter the mammalian host. Although OspA is not a major antigen produced in the mammal, the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01307-06
更新日期:2007-06-01 00:00:00
abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1055-1060.1992
更新日期:1992-03-01 00:00:00
abstract::Escherichia coli O45 isolates associated with swine postweaning diarrhea in Québec were characterized with respect to virulence determinants genetically and investigated for their attaching and effacing (A/E) activities by experimental inoculation of gnotobiotic piglets and by the HEp-2 cell adherence assay. All of 32...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4153-4159.1994
更新日期:1994-10-01 00:00:00
abstract::Endotoxin-resistant C3H/HeJ mice were used to test the hypothesis that a macrophage product, possibly interleukin-1, might mediate the depression of liver cytochrome P-450-dependent drug metabolism in endotoxin-treated mice. Depression of liver drug metabolism by endotoxin was observed in normal mice (C3H/HeN) but not...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.837-840.1986
更新日期:1986-12-01 00:00:00
abstract::Group B streptococci (GBS) adhere to surface receptors present on epithelial cells; these receptors include fibronectin and laminin. To identify other possible receptors, plasma membranes from A549 cells, a respiratory tract epithelial cell line, were prepared. These plasma membranes were tested in a protein blot anal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2129-2134.2000
更新日期:2000-04-01 00:00:00
abstract::We examined the effect of host age and health status on the adherence of mannose-sensitive piliated Escherichia coli K1 to human oral epithelial cells. Mannose-sensitive piliated bacteria adhered in comparable numbers to newborn, older infant, and adult cells (125 +/- 61, 198 +/- 54, and 139 +/- 69 bacteria per cell, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.299-301.1984
更新日期:1984-07-01 00:00:00
abstract::In this study, we report on a novel approach based on modification of the intestinal surface to prevent diarrhea caused by enterotoxigenic Escherichia coli (ETEC). The removable intestinal tie adult rabbit diarrhea (RITARD) model was used to test the efficacy of an enteric-coated protease preparation (Detach; Enzacor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3708-3714.1991
更新日期:1991-10-01 00:00:00
abstract::Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies, but was more pronou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.84-90.1977
更新日期:1977-01-01 00:00:00
abstract::An established cell line (Vero) defective in interferon production was used to evaluate the role of interferon in chronic rubella virus infections of cell cultures. Inoculation of Vero cells with a low multiplicity of virus resulted in the development of carrier cultures which had the characteristics of a regulated in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.2.132-138.1970
更新日期:1970-08-01 00:00:00
abstract::The level of type-specific antigen (that covalently associated with the cell wall peptidoglycan and that released extracellularly) synthesized by virulent and avirulent strains of type III group B streptococci was quantitated and compared. Additionally, the effect of the physiological age of the cells and the influenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.217-221.1984
更新日期:1984-05-01 00:00:00
abstract::Guinea pigs sensitized by repeated skin testing with potent, merthiolated coccidioidin, equal to Smith standard, exhibited erythema, edema, and an induration-like reaction to intradermal injection of the preparation. No statistically significant migration inhibition of peritoneal exudate macrophages from these animals...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.2.226-230.1973
更新日期:1973-02-01 00:00:00
abstract::Treatment with nontoxic monophosphoryl lipid A (MPL), which was derived from a polysaccharide-deficient, heptoseless Re mutant of Salmonella typhimurium, was found to inactivate suppressor T-cell activity, as evidenced by a decrease in the degree of low-dose immunological paralysis expressed and an increase in the mag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1076-1083.1988
更新日期:1988-05-01 00:00:00
abstract::While investigating the requirement for phagosomal alkalinization in the host defense against pulmonary aspergillosis, we observed high morbidity of p47(phox)(-/-) mice infected with pH-insensitive Aspergillus nidulans mutants despite a paucity of fungal growth. Fatal infection also resulted from a normally avirulent ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5204-5207.2005
更新日期:2005-08-01 00:00:00
abstract::Monoclonal antibodies against 12 of the 17 IATS serotype strains of Pseudomonas aeruginosa were produced. Eighty-seven hybridoma clones were isolated, and the antibodies secreted were found to be reactive with both Formalin-fixed whole cells and purified lipopolysaccharide of homologous strains in enzyme-linked immuno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.5.1051-1057.1987
更新日期:1987-05-01 00:00:00
abstract::One- to three-day-old guinea pigs were inoculated intranasally with the chlamydial agent of guinea pig inclusion conjunctivitis. Physical signs of infection included a marked increase in respiration rate on days 5 to 10 of infection and radiographic evidence of pneumonia on day 6. When animals were killed at various t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.153-158.1985
更新日期:1985-04-01 00:00:00
abstract::Fifty Borellia burgdorferi strains isolated from humans and ticks in Europe and the United States were analyzed by multilocus enzyme electrophoresis. Eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. Ten loci were polymorphic. The average number of alleles per loc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1677-1683.1992
更新日期:1992-04-01 00:00:00
abstract::The precise role of Leishmania glycoconjugate molecules including phosphoglycans (PGs) and lipophosphoglycan (LPG) on host cellular responses is still poorly defined. Here, we investigated the interaction of Leishmania major LPG2 null mutant (lpg2(-)), which lacks both PGs and LPG, with dendritic cells (DCs) and the s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01447-08
更新日期:2009-08-01 00:00:00
abstract::Salmonella infection can cause gastroenteritis in healthy individuals or a serious, systemic infection in immunocompromised patients and has a global impact. CD4 Th1 cells represent the main lymphocyte population that participates in bacterial clearance during both primary and secondary infections in mice of the H-2b ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00588-19
更新日期:2019-10-18 00:00:00
abstract::Fimbrial adhesins that mediate attachment to host cells are produced by most virulent Escherichia coli isolates. These virulence factors play an important role in the initial stages of bacterial colonization and also in determination of the host and tissue specificity. Isolates belonging to serotype O78 are known to c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5901-5907.2000
更新日期:2000-10-01 00:00:00
abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3272-3279.1988
更新日期:1988-12-01 00:00:00
abstract::The adherence and aggregation properties of 46 human oral Streptococcus salivarius isolates were examined. A total of 41% of the isolates aggregated with whole human saliva, 50% aggregated with human erythrocytes, and 85% adhered to human buccal epithelial cells. Strains that aggregated with saliva and erythrocytes us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1391-1396.1974
更新日期:1974-12-01 00:00:00
abstract::Shigella flexneri 2a is an invasive enteric pathogen that may produce diarrhea when ingested by human beings and subhuman primates. We have previously shown that shigella diarrhea correlates with water and electrolyte transport abnormalities in the jejunum and colon. Dysentery alone is associated only with colonic tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.368-371.1976
更新日期:1976-08-01 00:00:00
abstract::Chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. By stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. However, in patient this condition is most typically found in lungs damaged by other factors, such ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2936-2942.1992
更新日期:1992-07-01 00:00:00
abstract::The fibronectin-binding proteins (FnBPs) of Staphylococcus aureus are believed to be implicated in the pathogen's adherence to and colonization of bovine mammary glands, thus leading to infectious mastitis. In vitro studies have shown that FnBPs help the adhesion of the pathogen to bovine mammary epithelial cells. How...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2292-2295.2003
更新日期:2003-04-01 00:00:00
abstract::Each of the serotypes of Haemophilus influenzae (types a to f) may colonize the respiratory tract of humans, but only type b strains commonly cause invasive systemic infections. To investigate the role of complement in immunity to encapsulated non-type b strains, rats were depleted of C3 with cobra venom factor and ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.759-763.1982
更新日期:1982-03-01 00:00:00