Abstract:
:Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes the disease human monocytic ehrlichiosis. The pathogen also causes disease in several other vertebrates, including dogs and deer. In this study, we assessed two clonally purified E. chaffeensis mutants with insertions within the genes Ech_0379 and Ech_0660 as vaccine candidates in deer and dogs. Infection with the Ech_0379 mutant and challenge with wild-type E. chaffeensis 1 month following inoculation with the mutant resulted in the reduced presence of the organism in blood compared to the presence of wild-type infection in both deer and dogs. The Ech_0660 mutant infection resulted in its rapid clearance from the bloodstream. The wild-type infection challenge following Ech_0660 mutant inoculation also caused the pathogen's clearance from blood and tissue samples as assessed at the end of the study. The Ech_0379 mutant-infected and -challenged animals also remained positive for the organism in tissue samples in deer but not in dogs. This is the first study that documents that insertion mutations in E. chaffeensis that cause attenuated growth confer protection against wild-type infection challenge. This study is important in developing vaccines to protect animals and people against Ehrlichia species infections.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nair AD,Cheng C,Jaworski DC,Ganta S,Sanderson MW,Ganta RRdoi
10.1128/IAI.00487-15subject
Has Abstractpub_date
2015-07-01 00:00:00pages
2827-35issue
7eissn
0019-9567issn
1098-5522pii
IAI.00487-15journal_volume
83pub_type
杂志文章abstract::In vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal B-cell activators (PBA). We reasoned that if PBA were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.823-830.1988
更新日期:1988-04-01 00:00:00
abstract::In order to ascertain if Cryptococcus neoformans components can induce interleukin-6 (IL-6) production, we stimulated human whole blood with purified capsular products. Their potencies in stimulating IL-6 release were mannoproteins > galactoxylomannan = glucuronoxylomannan > alpha(1-3)glucan. IL-6 production was tumor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2454-2456.1997
更新日期:1997-06-01 00:00:00
abstract::The capacity of the intracellular pathogen Listeria monocytogenes to activate the alternative pathway of human complement was examined. Incubation of L. monocytogenes with human serum in optimal conditions (20% Mg2+EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid]-chelated serum) consumed (3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5134-5139.1993
更新日期:1993-12-01 00:00:00
abstract::The binding of (125)I-labeled S component to rabbit polymorphonuclear leukocytes was found to be concentration dependent and saturable at 37 degrees C. Scatchard analysis of the binding curve gave a straight line, indicating that S component binds to a single population of sites. The dissociation constant, K(D), deriv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.362-367.1981
更新日期:1981-11-01 00:00:00
abstract::Sublethal infection of mice with recombinant Listeria monocytogenes expressing a model epitope in either secreted or nonsecreted form results in similar CD8(+) T-cell priming. Since nonsecreted bacterial proteins have no obvious access to the endogenous major histocompatibility complex (MHC) class I presentation pathw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2196-2204.2000
更新日期:2000-04-01 00:00:00
abstract::Monoclonal antibody WM-25 inhibited the in vitro growth of 13 F38 isolates from goats with contagious caprine pleuropneumonia but not 7 heterologous mycoplasma isolates representing four different species. In contrast to results with polyclonal antisera, growth inhibition by monoclonal antibody WM-25 was specific for ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.3219-3220.1987
更新日期:1987-12-01 00:00:00
abstract::Borrelia burgdorferi produces a mitogen for murine B lymphocytes which can be measured in vitro by polyclonal stimulation of proliferation and immunoglobulin production (R. Schoenfeld, B. Araneo, Y. Ma, L. Yang, and J. J. Weis, Infect. Immun. 60:455-464, 1992). Sonicated B. burgdorferi cells also stimulated IL-6 produ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3033-3041.1992
更新日期:1992-08-01 00:00:00
abstract::Yersinia pestis, the agent of plague, has arisen from a less virulent pathogen, Yersinia pseudotuberculosis, by a rapid evolutionary process. Although Y. pestis displays a large number of virulence phenotypes, it is not yet clear which of these phenotypes descended from Y. pseudotuberculosis and which were acquired in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5892-5899.2003
更新日期:2003-10-01 00:00:00
abstract::A common concern with many autoimmune diseases of unknown etiology is the extent to which tissue T-lymphocyte infiltrates, versus a nonspecific infiltrate, reflect a response to the causative agent. Lyme arthritis can histologically resemble rheumatoid synovitis, particularly the prominent infiltration by T lymphocyte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.3.1092-1099.1998
更新日期:1998-03-01 00:00:00
abstract::The complement system plays a key role in resistance to cryptococcosis. In the present study, we examined several factors that influence the binding of C3 cleavage fragments to Cryptococcus neoformans. Binding of C3 was determined by using normal human serum supplemented with 125I-labeled C3. Incubation of encapsulate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.1-5.1986
更新日期:1986-04-01 00:00:00
abstract::Vibrio cholerae is the causative bacteria of the diarrheal disease cholera, but it also persists in aquatic environments, where it displays an expression profile that is distinct from that during infection. Upon entry into the host, a tightly regulated circuit coordinates the induction of two major virulence factors: ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00707-16
更新日期:2016-12-29 00:00:00
abstract::Tox-+ staphylococcal strains, as opposed to Tox-minus strains, produce epidermal exfoliation within 18 h after direct subcutaneous or intraperitoneal injection into newborn mice. The extracellular product responsible for exfoliation is termed exfoliative toxin (ET). When culture supernatant fluid from the plasmid-cure...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.1.193-197.1975
更新日期:1975-07-01 00:00:00
abstract::The yields of exotoxin A in Pseudomonas aeruginosa cultures were influenced by the concentration of iron in the culture media. When the iron concentration of the culture media was increased from 0.05 to 1.5 microgram/ml, there was at least a 90% decrease in exotoxin A (measured both by enzymatic activity and by mouse ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.785-791.1978
更新日期:1978-03-01 00:00:00
abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2042-2047.1990
更新日期:1990-07-01 00:00:00
abstract::Antigen-specific T-cell responses and histopathological changes were studied in mice experimentally inoculated with Borrelia burgdorferi B31. Inbred mice with different H-2 haplotypes and/or different genetic backgrounds were inoculated with B. burgdorferi organisms and tested for antigen-specific T-cell responses in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.41-47.1989
更新日期:1989-01-01 00:00:00
abstract::Monoclonal antibodies (MAbs) specific for Plasmodium falciparum rhoptry-associated protein 1 (RAP-1) were generated and tested for inhibition of parasite growth in vitro. The majority of indirect immunofluorescence assay (IFA)-positive MAbs raised against recombinant RAP-1 positions 23 to 711 (rRAP-1(23-711)) recogniz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2558-2568.2001
更新日期:2001-04-01 00:00:00
abstract::Since melanin is considered a virulence factor in Cryptococcus neoformans, its suppression at 37 degrees C has been perplexing. We now show an opposite thermal regulation of superoxide dismutase (SOD), consistent with a compensatory mechanism. Moreover, we demonstrate normal SOD and catalase levels in albino, oxygen-s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4085-4086.1994
更新日期:1994-09-01 00:00:00
abstract::Pseudomonas aeruginosa can invade corneal epithelial cells and translocates multilayered corneal epithelia in vitro, but it does not penetrate the intact corneal epithelium in vivo. In healthy corneas, the epithelium is separated from the underlying stroma by a basement membrane containing extracellular matrix protein...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00111-09
更新日期:2009-08-01 00:00:00
abstract::ZnCl2 over a very narrow concentration range was found to be mitogenic for hamster lymph node cells but not for thymocytes or splenocytes. Maximal leucine, [3H]uridine, and [3H]thymidine incorporation. Addition of 10 micron ZnCl2 was found to greatly enhance the stimulation observed with the B-lymphocyte mitogen lipop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.457-461.1978
更新日期:1978-02-01 00:00:00
abstract::Virulent Treponema pallidum organisms, extracted from infected rabbit testes, were subjected to velocity sedimentation in discontinuous gradients of Hypaque, a high density, low viscosity material. After centrifugation of extracts at 20 C for 45 min at 100,000 x g, treponemes separated into two distinct bands based up...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1062-1067.1974
更新日期:1974-11-01 00:00:00
abstract::FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00854-12
更新日期:2012-12-01 00:00:00
abstract::A set of four monoclonal antibodies was produced against a highly purified pepsin extract of type 5 streptococcal M protein. Three of the four antibodies cross-reacted with purified M proteins from heterologous serotypes and opsonized the respective heterologous organisms. Our studies suggest that monoclonal antibodie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.267-269.1984
更新日期:1984-10-01 00:00:00
abstract::A clone expressing a 58-kDa protein reactive with human convalescent-phase serum was isolated from a recombinant library of Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Sequencing identified two open reading frames, one encoding a 10.3-kDa polypeptide consisting of 94 amino acids and another encod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3536-3539.1993
更新日期:1993-08-01 00:00:00
abstract::Of 202 captive hares studied, many of which had lesions on their external genital organs or testicular atrophy or both, 27% had positive serological tests for syphilis although dark-field examination of extracts of atrophic testes was negative. A total of 12 hares that were nonreactive for the serological test for syp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.231-234.1980
更新日期:1980-01-01 00:00:00
abstract::The agent of Lyme disease, Borrelia burgdorferi, has a number of outer membrane proteins that are differentially regulated during its life cycle. In addition to their physiological functions in the organism, these proteins also likely serve different functions in invasiveness and immune evasion. In borreliae, as well ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05836-11
更新日期:2012-01-01 00:00:00
abstract::Tuberculosis remains the leading cause of death among infectious diseases, accounting for more than two million deaths annually. The incidence of the disease is increasing globally, partially because of the resurgence of drug-resistant strains of Mycobacterium tuberculosis. Calixarenes are macrocyclic oligomers, some ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6318-6323.2004
更新日期:2004-11-01 00:00:00
abstract::Growth in colonies with type 1 morphology and the presence of pili are characteristics that have been associated with virulence of gonococci for humans. To determine whether the presence of pili per se might be responsible for colony type 1 morphology, the relationship of pili to colony type was examined in various sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.594-600.1977
更新日期:1977-02-01 00:00:00
abstract::Dialyzable Lawrence-type transfer factor was prepared from the spleen cells of CF1 mice inoculated with Coccidioides immitis- and Candida albicans-killed vaccines and with live Mycobacterium tuberculosis vaccine (BCG). These preparations were shown to transfer antigen-specific cell-mediated immunity to naive mice, as ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.258-262.1977
更新日期:1977-04-01 00:00:00
abstract::Neuronal dysfunction can occur in the course of sepsis without meningitis. Sepsis-associated neuronal damage (SAND) was observed in the hippocampus within hours in experimental pneumococcal bacteremia. Intravascular challenge with purified bacterial cell wall recapitulated SAND. SAND persisted in PAFr(-/-) mice but wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00022-06
更新日期:2006-07-01 00:00:00
abstract::Sixty-one Burkholderia cepacia isolates from patients with cystic fibrosis (CF) and four plant isolates were screened for production of the siderophores salicylic acid (SA), pyochelin, cepabactin, and ornibactins and fingerprinted by a PCR-based randomly amplified polymorphic DNA (RAPD) method. Of the 24 RAPD types de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.874-877.1998
更新日期:1998-02-01 00:00:00